Preparation of a new resource food-arabinogalactan and its protective effect against enterotoxicity in IEC-6 cells by inhibiting endoplasmic reticulum stress.

Plant polysaccharides can be used as bioactive natural polymers that provide health benefits, however high molecular weight neutral polysaccharides have not shown good bioactivity. In this study, high molecular weight neutral arabinogalactan was isolated and structurally characterized to investigate it antioxidant activity against IEC-6 cells. In this study, a neutral polysaccharide (AG-40-I-II) was obtained from the roots of Larix gmelinii (Rupr.) Kuzen. and purified using ethanol fractional precipitation and purification on a DEAE-52 cellulose column and a Superose 12 gel filtration column. The structural characteristics of AG-40-I-II was detected by chemical and spectroscopic methods. The results showed that the average molecular weight of AG-40-I-II was 18.6 kDa, the main chain was composed of →4)-ß-D-Gal-(1, â†’ 4, 6)-ß-D-Gal-(1 and →4)-ß- D-Glc-(1, the side chain is composed of T-ß-L-Araf(1 â†’ 6). The effect of AG-40-I-II on H2O2-induced IEC-6 cell injury was determined by MTT method. Besides, AG-40-I-II could reduce the level of MDA and increase SOD activity on IEC-6 cells, which could significantly inhibit the production of ROS. Importantly, AG-40-I-II inhibited the splicing of XBP1 by IRE1α through the ERS pathway and reduced the cell apoptosis induced by H2O2. In summary, the results of this study indicate that AG-40-I-II, as a natural source of plant polysaccharides, has good antioxidant activity, and is expected to become a safe plant source of natural antioxidants, which has great potential in biomedicine potential.

Could peony seeds oil become a high-quality edible vegetable oil? The nutritional and phytochemistry profiles, extraction, health benefits, safety and value-added-products.

Edible oil provides essential nutrients for human body and plays a very important role in human health. Looking for edible oil resource plants with high content and high-quality oil plays an important role in ensuring the adequate supply of edible oil. Peony seeds oil (PSO) was approved as a new resource food and became an edible vegetable oil for its rich content of fatty acids, total unsaturated fatty acids, linoleic acid and linolenic acid. In addition, PSO also contains high contents of squalene, tocopherol, phytosterol and plant polyphenol. PSO has been reported to present various health benefits including antioxidation, blood lipid reduction, hepatoprotection, immunity regulation, blood sugar control, neuroprotection, anti-inflammatory, cytotoxic activity, therapeutic effects on scald, uvioresistant effects and so on. Toxicity studies showed PSO had great safety without any toxic and side effects, and could be processed to produce PSO microcapsule, microemulsion, nanoemulsion gel, biscuit, daily chemical products, soft capsule and other high value-added-products. Therefore, the present article aims to summarize the research findings regarding to the nutritional and phytochemistry profiles, the extraction methods, health benefits, safety and the high value-added-products of PSO. These informations laid a good foundation for the in-depth research and development of PSO, and also proved that PSO was a high-quality edible vegetable oil with health value. With the deepening of research, the studies on PSO will be more and more comprehensive. It believes that PSO will become an important functional edible oil, and will be more widely used as an important functional food and linolenic acid supplement.