RESUMO
Brassinosteroids (BRs) are plant hormones that are essential in plant growth and development. BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1 ASSOCIATED RECEPTOR KINASE 1 (BAK1), which are located on the plasma membrane, function as co-receptors that accept and transmit BR signals. PROHIBITIN 3 (PHB3) was identified in both BRI1 and BAK1 complexes by affinity purification and LC-MS/MS analysis. Biochemical data showed that BRI1/BAK1 interacted with PHB3 in vitro and in vivo. BRI1/BAK1 phosphorylated PHB3 in vitro. When the Thr-80 amino acid in PHB3 was mutated to Ala, the mutant protein was not phosphorylated by BRI1 and the mutant protein interaction with BRI1 was abolished in the yeast two-hybrid assay. BAK1 did not phosphorylate the mutant protein PHB3T54A . The loss-of-function phb3 mutant showed a weaker BR signal than the wild-type. Genetic analyses revealed that PHB3 is a BRI1/BAK1 downstream substrate that participates in BR signalling. PHB3 has five homozygous in tomato, and we named the closest to AtPHB3 as SlPHB3.1. Biochemical data showed that SlBRI1/SlSERK3A/SlSERK3B interacted with SlPHB3.1 and SlPHB3.3. The CRISPR-Cas9 method generated slphb3.1 mutant led to a BR signal stunted relatively in tomatoes. PHB3 is a new component of the BR signal pathway in both Arabidopsis and tomato.
Assuntos
Proteínas de Arabidopsis , Arabidopsis , Solanum lycopersicum , Arabidopsis/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Brassinosteroides/metabolismo , Solanum lycopersicum/genética , Proteínas Quinases/metabolismo , Fosforilação , Proteínas de Arabidopsis/metabolismo , Cromatografia Líquida , Proibitinas , Espectrometria de Massas em Tandem , Transdução de Sinais/fisiologia , Proteínas MutantesRESUMO
Prohibitins (PHBs) are composed of an obviously conserved protein family in eukaryotic cells. Despite the extensive and in-depth research of mammalian PHB1 and PHB2, the plant prohibitins functions are not completely elucidated and little is known about their mechanism of action. This review focuses on the current knowledge about the protein subcellular localization, interaction proteins and target genes of PHB3. Furthermore, we discussed the roles of PHB3 protein in plant growth and development, plant responses to abiotic or biotic stresses and its participation in phytohormonal signaling.
Assuntos
Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Desenvolvimento Vegetal , Proteínas Repressoras/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis , Retículo Endoplasmático/metabolismo , Genes de Plantas , Complexo de Golgi/metabolismo , Proibitinas , Ligação Proteica , Transporte ProteicoRESUMO
The root stem cell niche, which is composed of four mitotically inactive quiescent center (QC) cells and the surrounding actively divided stem cells in Arabidopsis, is critical for growth and root development. Here, we demonstrate that the Arabidopsis prohibitin protein PHB3 is required for the maintenance of root stem cell niche identity by both inhibiting proliferative processes in the QC and stimulating cell division in the proximal meristem (PM). PHB3 coordinates cell division and differentiation in the root apical meristem by restricting the spatial expression of ethylene response factor (ERF) transcription factors 115, 114, and 109. ERF115, ERF114, and ERF109 mediate ROS signaling, in a PLT-independent manner, to control root stem cell niche maintenance and root growth through phytosulfokine (PSK) peptide hormones in Arabidopsis.