Application of phytoaccumulation perspective of Monochoria hastate L. on fluoride contaminated water in hydroponic treatment: its statistical design and characterization studies.

The present research work approaches the accumulation of fluoride ions from contaminated water using an aquatic plant Monochoria hastate L. in hydroponic culture. A design of experiment (DOE) has been adopted and an analysis of variance has been conducted to establish the statistical significance of various process parameters. The different experimental factors are root and shoot (Factor A), fluoride concentration (Factor B), and experimental days (Factor C) largely influence the output response. Plants treated with 5 mg/L of fluoride solutions accumulated the highest concentration in root biomass 1.23 mg/gm, and shoot biomass 0.820 mg/gm, dry weight after 21 days' experimentation. The accumulation mechanism and potentiality of treated plants depend on root cells of the plasma membrane and energy-capturing molecules of adenosine triphosphate. Monochoria hastate L. root biomass was characterized to confirm the accumulation of fluoride ions in the experimented plants using scanning electron micrographs-energy dispersive spectrum (SEM-EDS), and Fourier transforms infrared analysis (FTIR) analysis.

The novelty of this study is the high fluoride accumulation efficiency in hydroponic treatment by Monochoria hastate L an excellent choice for phytoremediation technique. The Design of Experiment (DOE) has a good approach for the optimization of fluoride in the accumulation process. The maximum absorption of fluoride ions in root biomass is 1.23 mg/gm, and shoot biomass is 0.820 mg/gm, dry weight after 21 days of treatment. To know the fluoride ions in shoot and root biomass are characterized using scanning electron micrographs-energy dispersive spectrum (SEM-EDS), and Fourier transforms infrared analysis (FTIR).

Effectiveness of constructed wetland technology-treated industrial wastewater on the spinach (Spinacia oleracea) health risks and biochar efficiency.

In peri-urban areas, use of industrial wastewater for irrigation is a common practice. Industrial wastewater contains cadmium, chromium, lead, nickel, and other elements that deteriorate food quality and affect human health. Biochar has been proven to remediate heavy metal contaminated soil by reducing their mobility and bioavailability. A pot experiment was conducted to evaluate the efficiency of different levels of biochar on spinach growth with low heavy metal concentration and to minimize associated health issues. The experiment lasted two months and the treatments: Control (tap water), untreated and treated industrial wastewater and both in combination with biochar (0.5% and 1%) were applied in completely randomized design. Findings suggested that treated industrial wastewater with 1% biochar resulted in maximum plant height, shoot weight, chlorophyll contents (SPAD value), photosynthetic and transpiration rate. Biochar significantly reduced heavy metal mobility in soil due to its porous structure, high pH, higher CEC, and variety of surface functional groups. The cumulative hazard index (HI), hazard quotient, cancer risk, and total cancer risk (TCR) were calculated using method provided by US-EPA for each metal. All treatments had HI values of < 1, however applying 1% biochar significantly reduced the HI values to 2.00E-01 and 2.88E-01 in adults and children, respectively. TCR for all treatments was < 1, while treated industrial wastewater and biochar (1%) has significantly reduced to 1.55E-02 and 1.91E-03 for adults and children, respectively. Thus, it was determined that irrigation with industrial effluents caused toxicity in vegetables, which had a negative impact on human health. Biochar effectively mitigated metal toxicity in both soil and spinach plants that resulted in reduced health/cancer risk.

Effects of urban wastewater application on growth, biomass, nutrition, and heavy-metal accumulation of Populus nigra L. "62/154," P. alba L. "20/45," P. euramericana (Dode) Guinier "92/40," and Salix excelsa S.G. Gmel grown in heavy-metal contaminated soil.

Iran is located in a dry climate zone, and climate change has substantially reduced its precipitation and water resources. Reusing wastewaters from urban communities can meet some requirements for irrigation and fertilization of tree plantations in arid environments, leading to sustainable wastewater recycling, enhanced biomass production, and reduced land degradation. The objective of this study was to test the growth, biomass, nutrition, and heavy-metal accumulation of poplars [Populus nigra L. "62/154," P. alba L. "20/45," P. euramericana (Dode) Guinier "92/40"], and willow (Salix excelsa S.G. Gmel) in a pot experiment at four and eight months after planting when grown in soils irrigated with tap water (SITW) and wastewater (SIWW). After four months, SIWW treatment had no significant effect on growth, biomass, nor absorption of macronutrients. After eight months, SIWW treatment of poplars and willow significantly (p = 0.000) increased: (1) height, (2) leaf area, (3) root, stem, leaf, and total biomass, and (5) phytoextraction and phytoaccumulation of macro-/micro-nutrients and heavy metals in tree tissues, over trees receiving the SITW treatment. There were significant differences in growth, biomass, and accumulation of micronutrients and heavy metals in poplar versus willow tissues, with the highest biomass production and tissue-specific content of heavy metals in P. nigra trees, and the greatest total concentrations of heavy metals in P. alba and S. excelsa trees. In contrast, uptake of Fe, Cu, Ni, Cr and Pb were similar between poplar and willow, and phytoaccumulation of these elements was primarily in the roots. Leaf concentrations were highest for Zn and Mn. While P. nigra outperformed all other species overall, tolerance index (TI; defined as the tolerance to the heavy metals as calculated by the ratio of the biomass of SIWW trees relative to SITW trees) values exceeding 100% for all one-year-old poplar and willow trees demonstrated that they can be considered for planting in soil affected by urban wastewaters with similar contaminant profiles as in the current study.

Since the species would differ in their growth, biomass, and phytoremediation responses to the nutrient and heavy metal concentrations of the wastewater over time, this research is important for the development of silvicultural prescriptions of these fast-growing trees that support effective wastewater reuse strategies throughout heterogeneous landscapes and across variable human community resources and needs.

Potentially toxic elements contamination and its removal by aquatic weeds in the riverine system: A comparative approach.

Thamirabarani river acquires large untreated sewage effluents from the Tirunelveli and Thoothukudi districts of South Tamil Nadu. This study examined the concentration of trace elements in water, sediment, and phytoaccumulation potential of aquatic weeds viz., A. cristata, E. crassipes, S. natans, and P. stratiotes, growing along Srivaikundam dam of Thamirabarani river. The Pb, As, Hg, Cd, and Ni concentrations in water were slightly higher than the US Food and Drug Administration (USFDA) drinking water guidelines; however, their accumulation in sediment was below WHO's sediment quality guideline. This study concludes that the phytoaccumulation factor (PAF) and translocation factor (TF) was >1 in E. crassipes and A. cristata, representing them as hyperaccumulators, suitable for phytoremediation in polluted localities. E. crassipes, A. cristata, and S. natans accumulated (100-500 fold) higher trace elements concentrations than that present in the water. Also, the concentrations of trace elements found in the aquatic weeds were below the recommended levels for the critical plant range (CRP). These selected aquatic weeds are more suitable for plant hybridization to be modified as superbug plants.

Phosphorus fertilization and mycorrhizal colonization change silver nanoparticle impacts on maize.

Environmental risks of silver (Ag) nanoparticles (NPs) have aroused considerable concern, however, their ecotoxicity in soil-plant systems has yet not been well elaborated, particularly in agroecosystems with various fertility levels and soil biota. The aims of the present study were to determine AgNPs impacts on maize as influenced by mycorrhizal inoculation and P fertilization. A greenhouse pot experiment was conducted determine the effects of mycorrhizal inoculation with Rhizophagus intraradices and P fertilization (0, 20, and 50 P mg/kg soil, as Ca(H2PO4)2) on plant growth, Ag accumulation and physiological responses of maize exposed to AgNPs (1 mg/kg), or an equivalent Ag+. Overall, AgNPs and Ag+ did not significantly affect plant biomass and acquisition of mineral nutrients, activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), chlorophyll contents and photosystem (PS) II photochemical efficiency. In most cases, AgNPs and Ag+ caused similar Ag accumulation in plant tissues. P fertilization significantly increased Ag bioavailability and plant Ag accumulation, but only promoted the growth and P uptake of nonmycorrhizal plants. AM inoculation produced positive impacts on plant biomass, nutritional and physiological responses, but slightly affected extractable Ag in soil and Ag accumulation in plants. Mycorrhizal responses in plant growth and P uptake were more pronounced in the treatments without P but with Ag. By and large, AgNPs exhibited similar phytoavailability, phytoaccumulation and low phytotoxicity compared to Ag+, but higher fungitoxicity (i.e., lower root colonization). In conclusion, both AM inoculation and P fertilization can improve plant performance in the soil exposed to Ag, but P increases environmental risk of Ag. Our results indicate a beneficial role of arbuscular mycorrhizal fungi but a dual role of P in soil-plant systems exposed to AgNPs or Ag+.

Pseudo wastewater treatment by combining adsorption and phytoaccumulation on the Acrostichum aureum Linn. plant/activated carbon system.

In this study, the pseudo wastewater containing Zn, Fe, Cu ions was clean-up by a combination of physical adsorption onto activated carbon medium and phytoaccumulation using Acrostichum aureum Linn. plants. The adsorption capability of the activated carbon for the Fe, Cu, and Zn ions was 3.05, 3.72, and 2.85 mg·g - 1, respectively, at the saturation. The phytoaccumulation performance was proved by analyzing the individual residual ash collected after pyrolysis up to 1000 °C of the leaf, stem, and root of the plants. Thermal analyses of thermogravimetry data showed that the weight of the residual ash of the phytoremediated leaf, stem, and root of the plants was 37.0, 19.0, and 65.7 wt.%, respectively. Energy-dispersive X - ray spectroscopy determined the amount of Fe element in the residual ash of phytoremediated root is 7.05 wt.%, while that of the initial root is 1.18 wt.%. Conclusively, it can be proved that combining physical and biological processes is feasible to treat wastewater containing metal ions.

Increased antioxidative enzyme activity mediates the phytoaccumulation potential of Pb in four agroforestry tree species: a case study under municipal and industrial wastewater irrigation.

Wastewater used as irrigation water is causing heavy metal accumulation in the agro-ecosystems. A greenhouse study was conducted to compare the phytoaccumulation ability of four agroforestry tree species under different wastewater treatments. Three-month-old potted seedlings of Morus alba, Acacia nilotica, Acacia ampliceps, and Azadirachta indica were irrigation with tap water (C), municipal wastewater (MWW), and industrial wastewater (IWW). Results showed that MWW had a positive and IWW had a negative impact on biomass production in all the species. Acacia ampliceps showed the highest increment (65%) and showed the lowest decrease (5%) in total biomass under both MWW and IWW treatment. Pb concentration was also found highest in the leaves, stem and roots of Azadirachta indica (108.5, 46.2, 180.5 mg kg-1, respectively) under IWW. Production of H2O2 was highest in IWW treatment with almost 148% increase observed in Azadirachta indica. Similarly, the production of antioxidative enzymes (Superoxide dismutase, Catalase and Peroxidase) was also highest in Azadirachta indica under IWW. Therefore, results suggest that along with high increment in total biomass, both Acacia ampliceps and Azadirachta indica showed high Pb concentration and an effective antioxidative defense mechanism and thus, can be used for planting in soils irrigated with MWW and IWW.

Potential of Panicum aquanticum Poir. (Poaceae) for the phytoremediation of aquatic environments contaminated by lead.

Aquatic environments contaminated by lead (Pb) are a problem in many regions of world. Since Pb has high toxicity, the identification of species for phytoremediation is important for the recovery of these areas. Thus, the phytoremediation potential of Panicum aquaticum Poir. (Poaceae) was evaluated. The anatomical and physiological responses of P. aquaticum were assessed under different concentrations of Pb [0.0, 0.5, 1.0, 2.0, 4.0, and 8.0 mM of Pb(NO3)2]. Plant growth, anatomy of roots and leaves, root uptake, root to shoot translocation, and the concentration and accumulation of Pb in organs were analyzed. Regarding leaf anatomy, Pb treatment led to changes in epidermis thickness, stomatal density, stomatal diameter, and sclerenchymal area. Endoderm thickness was increased at the highest concentrations of Pb, which may be related to reduced translocation and shoot accumulation. The roots of P. aquaticum presented increased absorption (2279 µg g-1 DW-1 of Pb). In conclusion, P. aquaticum was found to have potential for the phytoremediation of areas contaminated with Pb.

Applying rhizobacteria consortium for the enhancement of Scirpus grossus growth and phytoaccumulation of Fe and Al in pilot constructed wetlands.

Pilot-scale constructed wetlands planted with Scirpus grossus, were used to investigate the effects of applying a three-rhizobacterial consortium (Bacillus cereus strain NII, Bacillus subtilis strain NII and Brevibacterium sp. strain NII) on the growth of S. grossus and also on the accumulation of iron (Fe) and aluminium (Al) in S. grossus. The experiment includes constructed wetlands with the addition of 2% of the consortium rhizobacteria and without the consortium rhizobacteria addition (acting as control). During each sampling day (0, 5, 10, 15, 20, 25, 30, 42, 72 and 102), plant height, concentration of Fe and Al and sand microbial community were investigated. The results for the constructed wetland with the addition of consortium rhizobacteria showed the growth of S. grossus increased significantly at 26% and 29% for plant height and dry weight, respectively. While the accumulation of Fe and Al in S. grossus were enhanced about 48% and 19% respectively. To conclude, the addition of the rhizobacteria consortium has enhanced both the growth of S. grossus and the metal accumulation. These results suggesting that rhizobacteria has good potential to restore Fe and Al contaminated water in general and particularly for mining wastewater.

Feasible Green Strategy for the Quantitative Bioaccumulation of Heavy Metals by Lemna minor: Application of the Self-Thinning Law.

This study involved the development of mathematical linear regression models to describe the relationships between mean plant biomass (M) and population density (D), M and frond diameter (L), frond numbers (N) and L of Lemna minor under different initial population densities (3200, 4450, and 6400 plants/m2), respectively, from the perspective of the self-thinning law. Our results revealed that the value of the allometric exponents for M and D were - 3/2. Further, the concentrations of Zn, Pb, Cu, Fe, and Ni accumulated in L. minor plants were 0.86, 0.32, 0.36, 0.62, and 0.39 mg/kg, respectively. Based on these developed equations and the heavy metal accumulations by L. minor, the phytoremediation capacity of L. minor was quantified via its frond diameters. Overall, the present study provides a cost-effective green method for managing the phytoremediation of heavy metal-contaminated aquatic environments.

Heavy metal phyto-accumulation in leafy vegetables irrigated with municipal wastewater and human health risk repercussions.

In this study, heavy metal phyto-accumulation potential of selected cultivars of two leafy vegetables on irrigation with municipal wastewater and human health risks were investigated. Municipal wastewater chemistry was recorded significantly different from groundwater control and led to the two-fold high enrichment of soil heavy metal contents (Ni, 19.46; Pb, 23.94; Co, 4.68; Cd, 1.4 in mg/kg, respectively). Interactive effects for phyto-accumulation of most heavy metals were also recorded significant at p < 0.001 in four vegetable cultivars. Heatmap revealed higher accumulation of heavy metals (Fe, Zn, Mn, Cu, Pb, Cr, Co) in spinach cultivars than lettuce cultivars creating elevated health risk index (HRI) and hazard index (HI) values for adults and children. Highest HI was recorded for Lahori palak (adults, 1.42; children, 2.58) and lowest for iceberg (adults, 0.04; children, 0.07). The NPK supplementation improved mineral composition of leafy vegetables within safer human health limits in control treatments. However, in municipal wastewater treatments, NPK fertilization decreased heavy metal uptake and phyto-accumulation in S2 (Lahori palak) than remaining vegetable cultivars leading to reduced health risk values. Because of higher heavy metal phyto-accumulation and health risks, cultivation of spinach cultivars must be discouraged in agro-ecologies receiving municipal wastes, whereas lettuce cultivars should be promoted.

Effective plant-endophyte interplay can improve the cadmium hyperaccumulation in Brachiaria mutica.

Soil contamination due to cadmium (Cd) is a ubiquitous environmental problem for which inexpensive remediation alternatives are required. Phytoaccumulation, the use of plants to extract and accumulate heavy metals from the contaminated environment, is such an alternative. In this study, we aimed at establishing effective plant-bacteria interplay between Brachiaria mutica and Cd-resistant endophytic bacteria eventually leading to improved phytoremediation. B. mutica was grown in a Cd-contaminated soil and inoculated with three Cd-tolerant endophytic bacteria individually as well as in combination. Plant physiological parameters, biomass production, bacterial colonization, and Cd-accumulation were observed at four different Cd exposures, i.e., 100, 200, 400 and 1000 mg kg-1 of soil. The combined application of endophytic bacteria was more effective as compared to their individual applications at all concentrations. Nevertheless, highest performance of consortium was seen at 100 mg Cd kg-1 of soil, i.e., root length was enhanced by 46%, shoot length by 62%, chlorophyll content by 40%, and dry biomass by 64%; which was reduced with the increase in Cd concentration. The bacterial population was highest in the root interior followed by rhizosphere and shoot interior. Concomitantly, plants inoculated with bacterial consortium displayed more Cd-accumulation in the roots (95%), shoots (55%), and leaves (44%). Higher values of BCFroot (> 1), and lower values for BCFshoot and TF (< 1) indicates capability of B. mutica to accumulate high amounts of Cd in the roots as compared to the aerial parts. The present study concludes that plant-endophyte interplay could be a sustainable and effective strategy for Cd removal from the contaminated soils.

Prospect of phytoaccumulation of arsenic by Brassica juncea (L.) in Bangladesh.

The phytoaccummulation of arsenic by Brassica juncea (L.) was investigated for varying concentrations selected within the range that is evident in Bangladeshi soil. B. juncea (Rai and BARI-11) was grown in the hydroponic media under greenhouse condition with different concentrations (0.5, 1.0, 15, 30, 50 and 100 ppm) of sodium arsenite. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used to analyze the data. Mapping of potential area of phytoaccumulation of arsenic by B. juncea was done using Geographic information system (GIS). Arsenic was detected at lower concentrations (0.5 and 1.0 ppm) only at root system of the plant. For higher concentrations (15, 30, and 50 ppm) arsenic was detected both in the root and shoot systems. The results suggested that at 15 and 50 ppm uptake was higher compared to 30 ppm. For 100 ppm of arsenic no plant growth was observed. In Bangladesh, where concentration of arsenic is at lower level and present only at rooting zone, B. juncea may be used for phytoaccumulation of arsenic keeping usual agronomic practices. However, for higher concentrations, B. juncea can be regarded as a good accumulator of arsenic where uptake of arsenic was up to 1% of total biomass of the plant.

Assessment of Ziziphus mauritiana grown on fly ash dumps: Prospects for phytoremediation but concerns with the use of edible fruit.

A field study was carried out on fly ash (FA) dumps of Panki Thermal Power Station to assess the phytoaccumulation of elements in various plant parts of edible fruit tree Ziziphus mauritiana. Of the twelve analyzed elements, the highest concentration was found for Fe followed by Mn > Se > Zn > Mo > Cu > Cr > Pb > Cd >Ni > As > Co in rhizospheric substrate of Z. mauritiana grown on FA dumps. Metal accumulation, bioconcentration factor, and translocation factor for each metal were calculated in various parts of the edible fruit tree. Significant variations of metal accumulations were observed among various plant parts. Accumulation of toxic elements was higher in roots, and it gradually declined toward the aerial parts of the plant corresponding to its distance from the ground. The concentration of some elements in fruit tree was found to be above prescribed limits in edible parts. Therefore, the present study suggested that additional care should be undertaken, if edible fruit trees are considered for phytoremediation or afforestation programs of FA dumps.

Vermiremediation of metal(loid)s via Eichornia crassipes phytomass extraction: A sustainable technique for plant amelioration.

Eichhornia crassipes (water hyacinth), imparts deficiency of soluble arsenic and other toxic metal (loid)s through rhizofiltration and phytoaccumulation. Without proper management strategy, this phytoremediation of metal (loid)s might fail and get reverted back to the environment, contaminating the nearby water bodies. This study, focused on bio-conversion of phytoremediating hyacinths, spiked with 100 times and greater arsenic, lead and cadmium concentrations than the average water contamination, ranging in 58.81 ±â€¯0.394, 16.74 ±â€¯0.367, 12.18 ±â€¯0.153 mg Kg-1arsenic, 18.95 ±â€¯0.212, 9.53 ±â€¯0.054, 6.83 ±â€¯0.306 mg kg-1 lead and 2.79 ±â€¯0.033, 1.39 ±â€¯0.025, 0.92 ±â€¯0.045 mg kg-1 cadmium, respectively in root, shoot and leaves, proving it's phytoaccumulation capacity. Next, these hyacinths has been used as a source of organic supplement for preparing vermicompost using Eisenia fetida following analysis of total metal content and sequential extraction. Control soil was having 134.69 ±â€¯2.47 mg kg-1 arsenic in compare to 44.6 ±â€¯0.91 mg kg-1 at premature stage of compost to 23.9 ±â€¯1.55 mg kg-1 at mature compost indicating sustainable fate of phytoremediated vermicompost. This vermiremediation of arsenic and other toxic elements, restricted the bioavailability of soil pollutants. Furthermore, processed compost amended as organic fertilizer, growing chickpea, coriander, tomato and chilli plant, resulted in negligible metal(loid)s in treated samples, enhancing also plant's growth and production.

Phytoremediation of organochlorine pesticides: Concept, method, and recent developments.

Rapid increase in industrialization of world economy in the past century has resulted in significantly high emission of anthropogenic chemicals in the ecosystem. The organochlorine pesticides (OCPs) are a great risk to the global environment and endanger the human health due to their affinity for dispersion, transportation over long distances, and bioaccumulation in the food chain. Phytoremediation is a promising technology that aims to make use of plants and associated bacteria for the treatment of groundwater and soil polluted by these contaminants. Processes known to be involved in phytoremediation of OCPs include phytoaccumulation, rhizoremediation, and phytotransformation. Vegetation has been accounted to considerably amplify OCP elimination from soil, in contrast to non-planted soil, attributable to both, uptake within plant tissues and high microbial degradation of OCP within the root zone. Developing transgenic plants is a promising approach to enhance phytoremediation capabilities. Recent advances in the application of phytoremediation technique for OCPs, including uptake by plants and plant-microbe association in the rhizosphere for the enhanced degradation and mineralization of these pollutants, is presented in this review. Additionally, some attempts to improve this technique using transgenesis and role of certain enzymes are also discussed.

Excessive sulphur accumulation and ionic storage behaviour identified in species of Acacia (Leguminosae: Mimosoideae).

BACKGROUND AND AIMS: Thiophores, which are typically desert gypsophytes, accumulate high (2-6 % S dry weight) sulphur concentrations and may possess unique tolerance to environmental stress factors, e.g. sulphate/metal toxicity, drought and salinity. Little is known of the prevalence of the behaviour or the associated physiological aspects. The aim of this study was to (a) determine the prevalence of thiophore behaviour in a group of Australian xerophytes; (b) identify elemental uptake/storage characteristics of these thiophores; and (c) determine whether the behaviour is constitutive or environmental. METHODS: The elemental composition of soils and the foliage of 11 species (seven genera) at a site in the Tanami Desert (NT, Australia) was compared and 13 additional Acacia species from other locations were examined for elevated calcium and sulphur concentrations and calcium-sulphur mineralization, thought to be particular to thiophores. KEY RESULTS: Acacia bivenosa DC. and 11 closely related species were identified as thiophores that can accumulate high levels of sulphur (up to 3·2 %) and calcium (up to 6.8 %), but no thiophores were identified in other genera occupying the same habitat. This behaviour was observed in several populations from diverse habitats, from samples collected over three decades. It was also observed that these thiophores featured gypsum (CaSO4·2H2O) crystal druses that completely filled cells and vascular systems in their dried phyllode tissues. CONCLUSIONS: The thiophores studied exhibit a tight coupling between sulphur and calcium uptake and storage, and apparently store these elements as inorganic salts within the cells of their foliage. Thiophore behaviour is a constitutive trait shared by closely related Acacia but is not highly prevalent within, nor exclusive to, xerophytes. Several of the newly identified thiophores occupy coastal or riparian habitats, suggesting that the evolutionary and ecophysiological explanations for this trait do not lie solely in adaptation to arid conditions or gypsiferous soils.

Assessment of metal toxicity and bioavailability in metallophyte leaf litters and metalliferous soils using Eisenia fetida in a microcosm study.

The leaf litters of tree species, Acacia pycnantha (Ap) and Eucalyptus camaldulensis (Ec), predominantly growing at an abandoned copper (Cu) mine and mine soils including controls, were assessed for determining the metal toxicity and bioavailability using earthworm species Eisenia fetida, in a microcosm. Significant reduction in body weight as well as mortality were observed when the worms were introduced into mine soil or its combination with mine Ap litter. Virtually, there were no juveniles when the worms were fed on substratum that contained mine soil or mine leaf litter. The extent of bioaccumulation was dependent on water-soluble fraction of a metal in soil. The accumulation of cadmium, lead and copper in worm tissue was significantly more in treatments that received mine soil with or without mine leaf litter. However, the tissue concentration of zinc did not differ much in earthworms irrespective of its exposure to control or contaminated samples. Mine leaf litter from Ec, a known Cu hyperaccumulator, was more hospitable to earthworm survival and juvenile than that of Ap litter. Validation of the data on bioaccumulation of metals indicated that the mine leaf litter significantly contributed to metal bioavailability. However, it was primarily the metal concentration in mine soil that was responsible for earthworm toxicity and bioavailability. Our data also indicate that detrivores like earthworm is greatly responsible for heavy metal transfer from mines into the ecosystem.

Agaricus bisporus compost improves the potential of Salix purpurea × viminalis hybrid for copper accumulation.

The aim of the study was to determine the ability of spent mushroom compost (SMC) from the production of Agaricus bisporus (A. bisporus) to stimulate the growth and efficiency of copper (Cu) accumulation by Salix purpurea × viminalis hybrid. Roots, shoots and leaves were analysed in terms of total Cu content and selected biometric parameters. Due to the absence of information regarding the physiological response of the studied plant, low molecular weight organic acids (LMWOAs), phenolic compounds and salicylic acid (SA) contents were investigated. The obtained results clearly demonstrate the effectiveness (usefulness) of SMC in promoting the growth and stimulation of Cu accumulation by the studied Salix taxon. The highest Cu content in roots and shoots was found at the 10% SMC addition (507±22 and 380±11 mg kg(-1) DW, respectively), while there was a reduction of the content in leaves and young shoots (109±8 and 124±7 mg kg(-1) DW, respectively). In terms of physiological response, lowered secretion of LMWOAs, biosynthesis of phenolic compounds and SA, as well as accumulation of soluble sugars in Salix leaves was observed with SMC addition. Simultaneously, an elevation of the total phenolic content in leaves of plants cultivated with SMC was observed, considered as antioxidant biomolecules.

From phytoaccumulation to post-harvest use of water fern for landfill management.

We examined the potential of Azolla japonica as a remediating plant for leachate channels and post-accumulation use as fertilizer for landfill slope. The harvested biomass of Azolla after one month grown in leachate was 254% that of the initial biomass and the predicted annual harvestable biomass of Azolla using a growth model was 32 times that of the initial biomass. Na, Fe, Mn, Mg, and P were accumulated in Azolla at very high concentrations. Such rapid increase of biomass and high accumulation rates suggest that this plant could be an excellent remediating plant. The post-harvest use of Azolla as compost was studied for the management and use of phytoaccumulating Azolla. Metal contents of Azolla compost were below permissible limits for co-composting material. Nitrogen, organic matter, P, and Mg content of the Azolla compost improved the soil condition of the landfill and enhanced ecophysiological responses of the plants. The application of Azolla compost can improve management of sanitary landfills, including the restoration of vegetation. Considering its ease of harvesting, high accumulation rates, harvestable biomass and suitability for composting, Azolla can provide a suitable solution for sustainable management of leachate channels and landfill slopes.