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1.
Plant J ; 119(1): 266-282, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38605581

RESUMO

Brassica crops are susceptible to diseases which can be mitigated by breeding for resistance. MAMPs (microbe-associated molecular patterns) are conserved molecules of pathogens that elicit host defences known as pattern-triggered immunity (PTI). Necrosis and Ethylene-inducing peptide 1-like proteins (NLPs) are MAMPs found in a wide range of phytopathogens. We studied the response to BcNEP2, a representative NLP from Botrytis cinerea, and showed that it contributes to disease resistance in Brassica napus. To map regions conferring NLP response, we used the production of reactive oxygen species (ROS) induced during PTI across a population of diverse B. napus accessions for associative transcriptomics (AT), and bulk segregant analysis (BSA) on DNA pools created from a cross of NLP-responsive and non-responsive lines. In silico mapping with AT identified two peaks for NLP responsiveness on chromosomes A04 and C05 whereas the BSA identified one peak on A04. BSA delimited the region for NLP-responsiveness to 3 Mbp, containing ~245 genes on the Darmor-bzh reference genome and four co-segregating KASP markers were identified. The same pipeline with the ZS11 genome confirmed the highest-associated region on chromosome A04. Comparative BLAST analysis revealed unannotated clusters of receptor-like protein (RLP) homologues on ZS11 chromosome A04. However, no specific RLP homologue conferring NLP response could be identified. Our results also suggest that BR-SIGNALLING KINASE1 may be involved with modulating the NLP response. Overall, we demonstrate that responsiveness to NLP contributes to disease resistance in B. napus and define the associated genomic location. These results can have practical application in crop improvement.


Assuntos
Brassica napus , Resistência à Doença , Doenças das Plantas , Proteínas de Plantas , Brassica napus/genética , Brassica napus/microbiologia , Brassica napus/metabolismo , Doenças das Plantas/microbiologia , Doenças das Plantas/genética , Doenças das Plantas/imunologia , Resistência à Doença/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Botrytis/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Peptídeos/metabolismo , Peptídeos/genética , Regulação da Expressão Gênica de Plantas , Mapeamento Cromossômico , Etilenos/metabolismo
2.
Proc Natl Acad Sci U S A ; 119(44): e2204698119, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36306329

RESUMO

War and crises affect mental health, social attitudes, and cultural norms, which can exacerbate the state of long-term insecurity. With decades of armed conflict, the Democratic Republic of Congo is one example, and violence has become normalized in civilian settings. In this study, we tested the effectiveness of the NETfacts health system, an integrated model of evidence-based individual trauma treatment (Narrative Exposure Therapy [NET]) and a trauma-informed community-based intervention (NETfacts). Alongside changes in mental health outcomes (posttraumatic stress disorder, depression, social disapproval, and shame) we also investigated change in attitudes, including rape myth acceptance, stigmatization of survivors of sexual violence, and skepticism about the reintegration of former combatants. To test whether the additional community intervention is superior to individual NET alone, we implemented a randomized controlled design with six villages and interviewed a sample of 1,066 community members. Our results demonstrate that the NETfacts health system in comparison with NET alone more effectively reduced rape myth acceptance and with it ongoing victimization and perpetration. Community members of the NETfacts group also presented with less stigmatizing attitudes against survivors of sexual violence. Skepticism about the reintegration of former combatants declined in both groups. NETfacts appears to have increased motivation to engage in individual treatment. Synergizing the healing effects of individual and collective trauma exposure, the NETfacts health system appears to be an effective and scalable approach to correct degrading or ignominious norms and restore functioning and mental health in postconflict communities.


Assuntos
Estupro , Delitos Sexuais , Transtornos de Estresse Pós-Traumáticos , Humanos , Estupro/psicologia , Transtornos de Estresse Pós-Traumáticos/terapia , Transtornos de Estresse Pós-Traumáticos/psicologia , Violência
3.
Planta ; 259(5): 122, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38619628

RESUMO

MAIN CONCLUSION: Overexpression of BnaC02.TPS8 increased low N and high sucrose-induced anthocyanin accumulation. Anthocyanin plays a crucial role in safeguarding photosynthetic tissues against high light, UV radiation, and oxidative stress. Their accumulation is triggered by low nitrogen (N) stress and elevated sucrose levels in Arabidopsis. Trehalose-6-phosphate (T6P) serves as a pivotal signaling molecule, sensing sucrose availability, and carbon (C) metabolism. However, the mechanisms governing the regulation of T6P synthase (TPS) genes responsible for anthocyanin accumulation under conditions of low N and high sucrose remain elusive. In a previous study, we demonstrated the positive impact of a cytoplasm-localized class II TPS protein 'BnaC02.TPS8' on photosynthesis and seed yield improvement in Brassica napus. The present research delves into the biological role of BnaC02.TPS8 in response to low N and high sucrose. Ectopic overexpression of BnaC02.TPS8 in Arabidopsis seedlings resulted in elevated shoot T6P levels under N-sufficient conditions, as well as an increased carbon-to-nitrogen (C/N) ratio, sucrose accumulation, and starch storage under low N conditions. Overexpression of BnaC02.TPS8 in Arabidopsis heightened sensitivity to low N stress and high sucrose levels, accompanied by increased anthocyanin accumulation and upregulation of genes involved in flavonoid biosynthesis and regulation. Metabolic profiling revealed increased levels of intermediate products of carbon metabolism, as well as anthocyanin and flavonoid derivatives in BnaC02.TPS8-overexpressing Arabidopsis plants under low N conditions. Furthermore, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) analyses demonstrated that BnaC02.TPS8 interacts with both BnaC08.TPS9 and BnaA01.TPS10. These findings contribute to our understanding of how TPS8-mediated anthocyanin accumulation is modulated under low N and high sucrose conditions.


Assuntos
Arabidopsis , Brassica napus , Fosfatos Açúcares , Trealose , Antocianinas , Arabidopsis/genética , Brassica napus/genética , Carbono , Flavonoides , Nitrogênio , Trealose/análogos & derivados , Técnicas do Sistema de Duplo-Híbrido
4.
J Exp Bot ; 2024 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-38809805

RESUMO

Plant can recruit beneficial microbes to enhance their ability to resist disease. Selenium is well established as a beneficial element in plant growth, but its role in mediating microbial disease resistance remained poorly understood. Here, we investigated the correlation between selenium, oilseed rape rhizosphere microbes and Sclerotinia sclerotiorum. Soil application of 0.5 and 1.0 mg/kg selenium significantly increased the resistance of oilseed rape to Sclerotinia sclerotiorum compared with no selenium application, and the disease inhibition rate was higher than 20%. The disease resistance of oilseed rape was related to rhizosphere microorganisms, and beneficial bacteria isolated from the rhizosphere inhibited Sclerotinia stem rot. Burkholderia cepacia, and synthetic community enhanced plant disease resistance through transcriptional regulation and activated plant-induced systemic resistance to protect plants. Besides, inoculation of isolated bacteria optimized the bacterial community structure of leaves and enriched beneficial microorganisms such as Bacillus, Pseudomonas and Sphingomonas. Bacillus isolated from the leaves were sprayed on the detached leaves, and it also performed a significant inhibition effect on Sclerotinia sclerotiorum. Overall, our results suggested that selenium drive plant rhizosphere microorganisms to increase resistance to Sclerotinia sclerotiorum in oilseed rape.

5.
J Exp Bot ; 2024 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-38776394

RESUMO

Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve the efficiency of agriculture and plant tissue culture-based practices. Here, we report a synthesis and structure-activity relationship study of novel urea derivatives concerning their CKX inhibitory activity. The best compounds showed sub-nanomolar IC50 values with maize ZmCKX1, the lowest value yet documented. Other CKX isoforms of maize (Zea mays) and Arabidopsis were also inhibited very effectively. The binding mode of four compounds was characterized based on high-resolution crystal complex structures. Using the soil nematode Caenorhabditis elegans, and human skin fibroblasts, key CKX inhibitors with low toxicity were identified. These compounds enhanced the shoot regeneration of Lobelia, Drosera, and Plectranthus, as well as the growth of Arabidopsis and Brassica napus. At the same time, a key compound (namely 82), activated a cytokinin primary response gene ARR5:GUS and cytokinin sensor TCSv2:GUS, without activating the Arabidopsis cytokinin receptors AHK3 and AHK4. This strongly implies that the effect of compound 82 is due to the upregulation of cytokinin signalling. Overall, this work presents highly effective and easily prepared CKX inhibitors with a low risk of environmental toxicity for further investigation of their potential in agriculture and biotechnology.

6.
Int J Legal Med ; 138(3): 1157-1164, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38092893

RESUMO

BACKGROUND: Female perpetrated sexual assault is under-represented in sexual assault research, and indeed possibly an underreported crime. The aim of this study is to address the lack of comprehensive data in relation to female perpetrated sexual assault attendances to the national sexual assault treatment unit network in the Republic of Ireland. METHODS: This is a cross-sectional study analysing the attendances of female perpetrated sexual assault attendances at the six sexual assault treatment units in the Republic of Ireland between 1 January 2017 and 31 December 2022. RESULTS: There were 95 attendances where the assailant (or one of the assailants) was identified as female. 62% (n=59) of these attendances involved a solo female assailant, 3.2% (n=3) where it was a multiple assailant assault with only female assailants and 34.7% (n=33) cases with male and female assailants. 74.7% (n=71) of victims identified as female, 24.2% (n=23) as male and 1.1% (n=1) as 'other'. The average age of attenders was 27.0 ± 10.7 years old. 54.7% (n=52) of attendances were within 72 hours of the assault. 52.6% (n=50) of these underwent a forensic examination. 30.5% (n=29) of incidents occurred in the assailant's home. 23% of assailants were described as a friend of the victim/survivor. 34.7% (n=33) sustained bodily injuries (genital and/or extra-genital). CONCLUSION: Female perpetrated sexual assault is a distinct entity when analysing attendances to the national sexual assault treatment unit network, representing just under 2% of all attendances. We have shown that those who experience these assaults are likely to be female, be assaulted by a single female perpetrator who is known to them and attend a sexual assault treatment unit within 72 hours of the assault. Awareness of the characteristics of these attendances will ultimately allow us to develop appropriate supports for these victims/survivors and to raise awareness of this type of crime.


Assuntos
Vítimas de Crime , Estupro , Delitos Sexuais , Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Irlanda/epidemiologia , Estudos Transversais
7.
Int J Legal Med ; 138(2): 571-581, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-37804334

RESUMO

Sexual violence is a pervasive global issue that affects individuals of all genders. However, the experiences of male survivors have often been marginalized and inadequately represented. Male rape, which encompasses several forms of sexual violence against men, remains a sensitive and under-discussed topic in academic literature and public discourse. This study presents a descriptive cross-sectional analysis based on data collected from the Legal Medicine Institute (IML-São Paulo, Brazil) between 2014 and 2017. The analysis includes 7386 reports of sexological examinations performed on male victims of alleged rape. The analysis reveals that a significant majority of rape reports involved victims under the age of 12 or 14, which is considered vulnerable rape by the Brazilian legislation. Regarding the examination of reported cases of abuse against men, it was observed that only the minority of these cases exhibited visible injuries consistent with rape or tested positive for the presence of spermatozoa in the perianal region. Since the absence of visible injuries or spermatozoa does not negate the possibility of rape, this work highlights the challenges in obtaining conclusive evidence, necessitating a comprehensive approach to investigate and prosecute these crimes, creating a more inclusive and supportive environment for all survivors of rape, irrespective of their gender.


Assuntos
Vítimas de Crime , Estupro , Delitos Sexuais , Humanos , Masculino , Feminino , Brasil/epidemiologia , Estudos Transversais , Estudos Epidemiológicos
8.
Arch Sex Behav ; 53(7): 2629-2652, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38886249

RESUMO

Rape myths-false but widely held beliefs that serve to deny and justify sexual aggression-present a major barrier to reporting and prevention of sexual violence in Vietnam and globally. Based on a parent study aimed at reducing sexual violence at two universities in Hanoi, we developed and assessed a contextualized measure of rape myths among young people in Vietnam. Items from previously validated rape myth acceptance (RMA) scales and data from qualitative research informed the development of 50 items, which were administered to Vietnamese 18-24-year-olds (n = 2,756 total, n = 1,798 cisgender women) via an anonymous link in February 2021. We used factor analysis to explore and test factor structure and multi-group factor analysis to assess measurement equivalence across gender. We calculated item-level discrimination and difficulty parameters and visualized information curves using item response theory analysis, informing the development of a short form. Four hypothesized subconstructs identified in the qualitative data emerged as factors: (1) "He didn't mean to"; (2) "She asked for it"; (3) "It wasn't really rape"; and (4) "Rape is a deviant event." A fifth factor, "She didn't protect herself," included four items from formative data. Confirming formative findings and prior literature, cisgender women had lower RMA than cisgender men, particularly on items related to victim-blaming. The Vietnamese Rape Myths Acceptance Scales were internally consistent and equivalent between cisgender men and women, capturing elements specific to the Vietnamese context and providing a tool for campus climate surveys and evaluations of sexual violence prevention programs.


Assuntos
Estupro , Humanos , Vietnã , Feminino , Masculino , Estupro/psicologia , Adulto Jovem , Adolescente , Inquéritos e Questionários , População do Sudeste Asiático
9.
Arch Sex Behav ; 53(3): 1001-1013, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38267597

RESUMO

Some people believe rape is just as serious as homicide, or more serious, contrary to law. We examined the prevalence of this belief and whether it reflects an individual's political ideology and moral foundations. Analyses were based on a national YouGov survey of 1,125 US adults gathered in 2021. We found that only 26% of respondents believed rape was less serious than homicide. Most (61%) believed rape and homicide were equally serious, while 13% believed rape was more serious. Social progressives (particularly progressive women) were more likely than social conservatives to view rape as more serious or just as serious as homicide. However, this tendency was partially offset by the tendency of social progressives to view harm as a key factor in judging the morality of a behavior. We suggest that social progressives view rape more seriously than social conservatives because of their concern for gender inequality, but this concern is partially offset by their concern with harm.


Assuntos
Estupro , Adulto , Humanos , Feminino , Homicídio , Princípios Morais , Política
10.
Bioethics ; 2024 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-38771718

RESUMO

This study examines girl child abuse in an internally displaced people's camp in north-central Nigeria and the response of community health workers. The conflict in Benue State is caused by religious differences between the natives (Tiv people) and the invading Fulani herdsmen. During these conflicts, women and girls were displaced, and they were kept in internally displaced persons (IDPs) located in different parts of the state. Literature has been extensively written on internal displacement in Nigeria, but none has been able to elucidate the health needs of girls and the various abuses girls and women are suffering in IDP camps. In this study, literature was extended (1) to explore the environment of the girl child in IDP camps, (2) to identify reasons for the abuse of the girl child in IDP camps, (3) to investigate the impacts of the abuse on the girl child in IDP camps and (4) to investigate how healthcare workers could be used to alleviate the plight of girl children in IDP camps. This is a qualitative case study, with data obtained from relevant academic literature and personal observation. The data were analysed using content analysis. Findings reveal that the girl child is suffering from psychological, economic and health challenges due to the various forms of abuse they are going through. Following the devastation in Benue State, healthcare workers from the community relocated to neighbouring states. There is a need to recall those healthcare workers and provide them with the necessary materials to assist girls in IDP camps. Recommendations are discussed.

11.
BMC Health Serv Res ; 24(1): 443, 2024 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-38594666

RESUMO

BACKGROUND: Rape, as an adverse incidence, leads to irreparable complications and consequences in women. Provision of health services to women survivors of rape requires catering for their real needs and identifying current deficits as well as barriers. The present study aimed to explore health system-related needs in women survivors of rape. METHODS: In the present qualitative study, the participants consisted of 39 individuals, including 19 women survivors of rape and 20 individuals with work experience in providing services to women survivors of rape. The participants were selected using the purposive sampling method with a maximum variation in Isfahan, Iran. Data were collected through in-depth interviews as well as field notes and were concurrently analyzed via conventional qualitative content analysis method. RESULTS: After analyzing the interviews, the health system-related needs of women survivors of rape were classified into two main categories: 1- The need for efficient medical care services with three sub-categories, namely "receiving services with respect for privacy and confidentiality", "non-judgmental behavior and approach", and "the need to receive empathy and the feeling of not being alone", and 2- The need for desirable conditions and structure to provide services with two sub-categories, namely "the need to receive comprehensive and integrated services", and "establishing specialized centers for providing services to survivors". CONCLUSIONS: Overall, explaining and highlighting the health system-related needs of women survivors of rape could provide a suitable basis for policy-making and planning according to their real needs. Receiving continuous services in separate centers with confidentiality and empathy could reduce the worries and concerns of women survivors of rape and help improve their health.


Assuntos
Estupro , Humanos , Feminino , Pesquisa Qualitativa , Projetos de Pesquisa , Programas Governamentais , Sobreviventes
12.
Pestic Biochem Physiol ; 200: 105785, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38582570

RESUMO

This study investigates the effects of chlorantraniliprole (CAP) pesticide stress on oilseed rape through comprehensive pot experiments. Assessing CAP residue variations in soil and oilseed rape (Brassia campestris L.), enzyme activities (POD, CPR, GST), and differential metabolites, we unveil significant findings. The average CAP residue levels were 18.38-13.70 mg/kg in unplanted soil, 9.94-6.30 mg/kg in planted soil, and 0-4.18 mg/kg in oilseed rape samples, respectively. Soil microbial influences and systemic pesticide translocation into oilseed rape contribute to CAP residue variations. Under the influence of CAP stress, oilseed rape displays escalated enzyme activities (POD, CPR, GST) and manifests 57 differential metabolites. Among these, 32 demonstrate considerable downregulation, mainly impacting amino acids and phenolic compounds, while 25 exhibit noteworthy overexpression, primarily affecting flavonoid compounds. This impact extends to 24 metabolic pathways, notably influencing amide biosynthesis, as well as arginine and proline metabolism. These findings underscore the discernible effects of CAP pesticide stress on oilseed rape.


Assuntos
Brassica napus , Praguicidas , ortoaminobenzoatos , Praguicidas/metabolismo , Brassica napus/metabolismo , Solo
13.
Plant Dis ; 108(6): 1688-1694, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38170446

RESUMO

Cover crops and flower strips are used in agricultural fields as part of integrated pest management strategies. However, their potential as secondary hosts of soilborne pathogens such as Sclerotinia sclerotiorum in oilseed rape cultivation is not fully comprehended. In the current study, we evaluated the effect of pathogen virulence on the development of Sclerotinia stem/leaf rot and sclerotia production in 33 plant species from 11 botanical families using two S. sclerotiorum isolates. Furthermore, the effect of sclerotial size on carpogenic germination was studied. Results showed that the pathogen's virulence significantly affected the occurrence and development of Sclerotinia stem/leaf rot and the subsequent production of sclerotia. Among all plant species tested, 26 were more susceptible to the highly aggressive S. sclerotiorum isolate, which produced more and bigger sclerotia in 17 species than the less aggressive isolate. Moreover, a stronger positive correlation was found between the relative lesion length of plants inoculated with the highly aggressive isolate and the number of sclerotia produced by this isolate (Spearman's rank correlation coefficient [rs] = 0.572; P = 0.004). Additionally, we found that larger and heavier sclerotia produced stipes and apothecia earlier and at a greater rate than smaller ones. The heavyweight class had the highest carpogenic germination rate (82.4%), followed by the average (67.2%) and lightweight classes (59.5%). Our findings highlight the need for further investigation into the potential risks associated with cover crops, weeds, and flower strips as secondary hosts of soilborne pathogens in agricultural fields.


Assuntos
Ascomicetos , Produtos Agrícolas , Flores , Doenças das Plantas , Plantas Daninhas , Ascomicetos/fisiologia , Ascomicetos/patogenicidade , Doenças das Plantas/microbiologia , Plantas Daninhas/microbiologia , Produtos Agrícolas/microbiologia , Flores/microbiologia , Virulência
14.
Int J Phytoremediation ; 26(4): 524-534, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-37641540

RESUMO

Mercury (Hg) pollution has seriously threatened the crop productivity and food security. In the present research, experiments were conducted to assess the influence of nanoscale sulfur/sulfur nanoparticles and the corresponding bulk and ionic sulfur forms on the growth and Hg accumulation of oilseed rape seedlings grown on Hg-contaminated soil, as well as the transformation of soil Hg fractions. The results showed a significant reduction in fresh biomass for seedlings grown on 80-200 mg/kg Hg-polluted soil after 30 days. At 120 mg/kg Hg treatment, 100-300 mg/kg sulfur nanoparticles (SNPs) application counteracted Hg toxicity more effectively compared to the corresponding bulk sulfur particles (BSPs) and ionic sulfur (sulfate) treatments. The seedlings treated with 120 mg/kg Hg + 300 mg/kg SNPs gained 54.2 and 56.9% more shoot and root biomass, respectively, compared to those treated with Hg alone. Meanwhile, 300 mg/kg SNPs application decreased Hg accumulation by 18.9 and 76.5% in shoots and roots, respectively, relative to Hg alone treatment.SNPs treatment caused more Hg to be blocked in the soil and accumulating significantly less Hg in plants as compared to other S forms. The chemical fractions of Hg in the soil were subsequently investigated, and the solubility of Hg was significantly decreased by applying SNPs to the soil. Especially 200-300 mg/kg SNPs treatments caused the ratio of the soluble/exchangeable and the specifically absorbed fraction to be the lowest, accounting for 1.95-4.13% of the total Hg of soil. These findings suggest that adding SNPs to Hg-contaminated soils could be an effective measure for immobilizing soluble Hg and decreasing the Hg concentration in the edible parts of crops. The results of the current study hold promise for the practical application of SNPs to Hg-contaminated farmland for better yields and simultaneously increasing the food safety.


The novelty of this study is the selection of oilseed rape and nanoscale sulfur (NS) or sulfur nanoparticles (SNPs) as nontoxic nanomaterial to counteract the Hg toxicity and accumulation. Oilseed rape was selected due to its wide adaptability to various environmental conditions and the high-value oil for human consumption and biofuels production. These advantages make oilseed rape a highly valuable crop for various applications. NS was selected due to its reported ability to limit the uptake of heavy metals in oilseed rape, rice, and wheat along with other crops and subsequently restrict the toxicity of heavy metals in these plants and improve food safety. In this study, we evaluated the growth, Hg accumulation, and the resulting toxicity in oilseed rape grown on Hg-contaminated soil, with or without amendments with NS. The outcomes from this study provided evidence of the significant potential of NS in preventing Hg bioaccumulation and improving crop yields in oilseed rape. This provides opportunity to use NS as an ideal non-GMO approach to limit toxic metals in crops.


Assuntos
Brassica napus , Mercúrio , Poluentes do Solo , Plântula/química , Biodegradação Ambiental , Solo , Enxofre , Poluentes do Solo/análise , Cádmio
15.
Int J Phytoremediation ; : 1-11, 2024 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-38597454

RESUMO

In the present study, experiments were conducted to assess the influence of nanoscale sulfur in the microbial community structure of metallophytes in Hg-contaminated rhizosphere soil for planting rapeseed. The results showed that the richness and diversity of the rhizobacteria community decreased significantly under Hg stress, but increased slightly after SNPs addition, with a reduction in the loss of Hg-sensitive microorganisms. Moreover, all changes in the relative abundances of the top ten phyla influenced by Hg treatment were reverted when subjected to Hg + SNPs treatment, except for Myxococcota and Bacteroidota. Similarly, the top five genera, whose relative abundance decreased the most under Hg alone compared to CK, increased by 19.05%-54.66% under Hg + SNPs treatment compared with Hg alone. Furthermore, the relative abundance of Sphingomonas, as one of the dominant genera for both CK and Hg + SNPs treatment, was actively correlated with plant growth. Rhizobacteria, like Pedobacter and Massilia, were significantly decreased under Hg + SNPs and were positively linked to Hg accumulation in plants. This study suggested that SNPs could create a healthier soil microecological environment by reversing the effect of Hg on the relative abundance of microorganisms, thereby assisting microorganisms to remediate heavy metal-contaminated soil and reduce the stress of heavy metals on plants.


In this manuscript, we first comprehensively investigated the changes in the rhizosphere microbial community structure of metallophytes in Hg-contaminated soil with SNPs addition, as well as the relationship between soil microbiology and plant resistance to Hg stress. Our results demonstrated that SNPs exhibit a significant advantage in improving rhizosphere microecology by increasing the abundance of beneficial rhizobacteria, thereby alleviating heavy metal toxicity, and promoting plant growth. This study is the first study describing the response of soil microorganisms coexposed to heavy metals and SNPs, providing valuable information for the potential use of SNPs to assist phytoremediation of toxic metal pollution and its impact on soil microbial communities.

16.
Sex Abuse ; 36(4): 486-506, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37455145

RESUMO

OBJECTIVE: Prior research has rarely focused on sexual motives (e.g., motives for having sex) when studying sexual violence perpetration prevention. The current study examined the role of sexual motives alongside other risk factors like alcohol expectancies in predicting sexual violence. METHOD: We analyzed data from 205 male college students; 36% reported sexual perpetration of some type. Participants completed a series of questionnaires in a randomized order, including: measures of prior sexual perpetration, sexual motives, rape myth acceptance, alcohol expectancies, and a measure of social desirability. Data were analyzed using a series of T-tests and logistic regressions. RESULTS: With one exception (coping motives), all sexual motives (intimacy, enhancement, self-affirmation, peer approval, and partner approval) were endorsed at higher levels by individuals who perpetrated sexual violence than those who did not (p < .05, Cohen's d = .25-.56). The partner approval motive significantly predicted sexual violence perpetration on its own. The enhancement motive, both independently and in interaction with alcohol expectancies for aggression, predicted sexual violence perpetration. Two other motives, intimacy and self-affirmation, were only significant in interaction with alcohol expectancies for aggression. CONCLUSION: All sexual motives were endorsed more frequently by those who perpetrated sexual violence than those who did not. Sexual motives had a complex interaction with alcohol expectancies in predicting sexual violence perpetration. The results suggest that intervention programs should emphasize healthy, consensual sexual relationships that do not involve alcohol.


Assuntos
Estupro , Delitos Sexuais , Humanos , Masculino , Comportamento Sexual , Violência , Agressão , Etanol
17.
Am J Community Psychol ; 73(3-4): 337-359, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38303610

RESUMO

Adolescents are sexually assaulted at remarkably high rates. Adolescents are also unique given the specific dynamics of adolescent sexual assault, their current stage in human development, their limited legal standing and rights, and their experiences navigating postassault services and resources. While literature exists within each of these domains, it is somewhat disconnected and overlooks how adolescents are often relegated to the margins in research and practice. The purpose of this integrative review is to (1) provide a more complete understanding of adolescent sexual assault and survivors' nuanced needs; (2) frame adolescent survivors as a too-often-overlooked oppressed group that researchers and responders must consider and center in their work, lest contribute to their further marginalization; and (3) inspire and orient social justice-minded scholar activists to various action steps to take to center and prioritize adolescents in research and practice. Through our intersectional feminist approach, we offer specific action steps for social justice-minded scholar activists to recenter adolescents in their research and practice.


Assuntos
Delitos Sexuais , Humanos , Adolescente , Vítimas de Crime/psicologia , Feminino , Justiça Social , Sobreviventes/psicologia , Masculino , Feminismo
18.
Violence Vict ; 39(1): 3-20, 2024 03 07.
Artigo em Inglês | MEDLINE | ID: mdl-38453368

RESUMO

Rape-related cognitions (typically defined as encompassing any number of cognitive constructs) are thought to play a role in sexual aggression. However, rape-related cognition scales often assess these cognitive constructs as one. The purpose of this study is to explore the factor structure of these measures using a sample of 191 community men. We found that items from the Rape Myth Acceptance, RAPE, and Illinois Rape Myth Acceptance (IRMA) scales formed one factor, which was significantly related to sexual aggression. We further found that four and six IRMA subscales were significantly related to past and likelihood of sexual aggression, respectively. Additionally, one IRMA subscale was independently related to past and likelihood of sexual aggression. The results are discussed in terms of implications and direction for future research.


Assuntos
Estupro , Masculino , Humanos , Estupro/psicologia , Agressão/psicologia , Estudantes/psicologia , Cognição , Comportamento Sexual/psicologia
19.
Violence Vict ; 39(1): 38-52, 2024 03 07.
Artigo em Inglês | MEDLINE | ID: mdl-38453367

RESUMO

The current study documents the correlates associated with the severity of sexual victimization among women enrolled in a 2-year community college. Comparisons between women with a history of severe sexual victimization (i.e., rape and attempted rape), moderate sexual victimization (i.e., unwanted contact and sexual coercion), and no history of sexual victimization revealed that women with a history of severe sexual victimization endorsed more drinks per week, increased problem drinking behavior, and more use of drug before sex and higher levels of self-protective dating behaviors compared with women with no history of sexual victimization. These findings suggest that programs should target the intersection of alcohol and drug use as correlates of sexual victimization among community college women.


Assuntos
Vítimas de Crime , Estupro , Delitos Sexuais , Feminino , Humanos , Comportamento Sexual , Etanol
20.
Violence Vict ; 39(2): 168-188, 2024 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-38955473

RESUMO

To more effectively respond to sexual assault in the United States, some jurisdictions have created sexual assault response teams (SARTs). SARTs involve members of multiple agencies tasked with responding to sexual assault within a jurisdiction, such as law enforcement, advocates, prosecutors, and sexual assault nurse examiners (SANEs). Despite hundreds of jurisdictions utilizing SARTs, and the Department of Justice establishing guidelines, SARTs vary across jurisdictions in their form and function. To clarify this, the current research outlines two studies to better understand the logistics and functioning of one SART. In Study 1, for over nearly 2 years, SART meetings were observed and notes were taken on representation of agencies, time spent on cases, and possible challenges. Overall, the SART met consistently, with representation of major stakeholders at each meeting. Stakeholder agencies remained consistent and most time spent in the meetings was on case review. In Study 2, 10 members of SART were interviewed to assess perceptions of SART. These interviews were independently qualitatively coded for both factual and thematic codes. Areas of focus included perceived logistics of the SART and expertise of members that make up an effective SART. Overall, on average, members had been part of the SART for 3.5 years, felt required to attend the meetings as part of their role, and shared that all members (i.e., law enforcement, district attorney, victim advocates, and SANEs) had expertise on differing subjects that effectively made the SART work well. Importantly, qualitative perceptions from members of the SART (Study 2) lined up with the independent observation of SART meetings (Study 1). Ultimately, the results from this body of research could delineate specific actions that a jurisdiction implementing a SART could utilize.


Assuntos
Aplicação da Lei , Delitos Sexuais , Humanos , Estados Unidos , Feminino , Vítimas de Crime , Entrevistas como Assunto , Masculino
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