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1.
Sensors (Basel) ; 23(9)2023 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-37177551

RESUMO

This paper proposes a neural-network-based framework using Convolutional Neural Network and Long-Short Term Memory (CNN-LSTM) for detecting faults and recovering signals from Hall sensors in brushless DC motors. Hall sensors are critical components in determining the position and speed of motors, and faults in these sensors can disrupt their normal operation. Traditional fault-diagnosis methods, such as state-sensitive and transition-sensitive approaches, and fault-recovery methods, such as vector tracking observer, have been widely used in the industry but can be inflexible when applied to different models. The proposed fault diagnosis using the CNN-LSTM model was trained on the signal sequences of Hall sensors and can effectively distinguish between normal and faulty signals, achieving an accuracy of the fault-diagnosis system of around 99.3% for identifying the type of fault. Additionally, the proposed fault recovery using the CNN-LSTM model was trained on the signal sequences of Hall sensors and the output of the fault-detection system, achieving an efficiency of determining the position of the phase in the sequence of the Hall sensor signal at around 97%. This work has three main contributions: (1) a CNN-LSTM neural network structure is proposed to be implemented in both the fault-diagnosis and fault-recovery systems for efficient learning and feature extraction from the Hall sensor data. (2) The proposed fault-diagnosis system is equipped with a sensitive and accurate fault-diagnosis system that can achieve an accuracy exceeding 98%. (3) The proposed fault-recovery system is capable of recovering the position in the sequence states of the Hall sensors, achieving an accuracy of 95% or higher.

2.
Sensors (Basel) ; 22(24)2022 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-36559964

RESUMO

Energy management strategies are vitally important to give full play to the energy-saving of the four-wheel drive electric vehicle (4WD EV). The cooperative output of multi-power components is involved in the process of driving and braking energy recovery of 4WD EV. This paper proposes a novel energy management strategy of dual equivalent consumption minimization strategy (D-ECMS) to improve the economy of the vehicle. According to the different driving and braking states of the vehicle, D-ECMS can realize the proportional control of the energy cooperative output among the multi-power components. Under the premise of satisfying the dynamic performance of the vehicle, the operating points of the power components are distributed more in the high-efficiency range, and the economy and driving range of the vehicle are optimized. In order to achieve the effectiveness of D-ECMS, MATLAB/Simulink is used to realize the simulation of the vehicle. Compared with the rule-based strategy, the economy of D-ECMS increased by 4.35%.

3.
Sensors (Basel) ; 21(10)2021 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-34067507

RESUMO

Recently, as Internet of Things systems have been introduced to facilitate diagnosis and treatment in healthcare and medical environments, there are many issues concerning threats to these systems' security. For instance, if a key used for encryption is lost or corrupted, then ciphertexts produced with this key cannot be decrypted any more. Hence, this paper presents two schemes for key recovery systems that can recover the lost or the corrupted keys of an Internet of Medical Things. In our proposal, when the key used for the ciphertext is needed, this key is obtained from a Key Recovery Field present in the cyphertext. Thus, the recovered key will allow decrypting the ciphertext. However, there are threats to this proposal, including the case of the Key Recovery Field being forged or altered by a malicious user and the possibility of collusion among participating entities (Medical Institution, Key Recovery Auditor, and Key Recovery Center) which can interpret the Key Recovery Field and abuse their authority to gain access to the data. To prevent these threats, two schemes are proposed. The first one enhances the security of a multi-agent key recovery system by providing the Key Recovery Field with efficient integrity and non-repudiation functions, and the second one provides a proxy re-encryption function resistant to collusion attacks against the key recovery system.

4.
Plant Mol Biol ; 103(1-2): 9-32, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32124177

RESUMO

KEY MESSAGE: In light of the available discoveries in the field, this review manuscript discusses on plant reproduction mechanism and molecular players involved in the process. Sperm cells in angiosperms are immotile and are physically distant to the female gametophytes (FG). To secure the production of the next generation, plants have devised a clever approach by which the two sperm cells in each pollen are safely delivered to the female gametophyte where two fertilization events occur (by each sperm cell fertilizing an egg cell and central cell) to give rise to embryo and endosperm. Each of the successfully fertilized ovules later develops into a seed. Sets of macromolecules play roles in pollen tube (PT) guidance, from the stigma, through the transmitting tract and funiculus to the micropylar end of the ovule. Other sets of genetic players are involved in PT reception and in its rupture after it enters the ovule, and yet other sets of genes function in gametic fusion. Angiosperms have come long way from primitive reproductive structure development to today's sophisticated, diverse, and in most cases flamboyant organ. In this review, we will be discussing on the intricate yet complex molecular mechanism of double fertilization and how it might have been shaped by the evolutionary forces focusing particularly on the model plant Arabidopsis.


Assuntos
Flores/fisiologia , Magnoliopsida/fisiologia , Polinização/fisiologia , Evolução Biológica , Gametogênese Vegetal , Óvulo Vegetal/fisiologia , Pólen/fisiologia , Sementes
5.
Sensors (Basel) ; 20(19)2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33023031

RESUMO

The development of launch and recovery technology is key for the application to the unmanned surface vehicle (USV). Also, a launch and recovery system (L&RS) based on a pneumatic ejection mechanism has been developed in our previous study. To improve the launch accuracy and reduce the influence of the sea waves, we propose a stacking model of one-dimensional convolutional neural network and long short-term memory neural network predicting the attitude of the USV. The data from experiments by "Jinghai VII" USV developed by Shanghai University, China, under levels 1-4 sea conditions are used to train and test the network. The results show that the stabilized platform with the proposed prediction method can keep the launching angle of the launching mechanism constant by regulating the pitching joint and rotation joint under the random influence from the wave. Finally, the efficiency and effectiveness of the L&RS are demonstrated by the successful application in actual environments.

6.
J Clean Prod ; 162: 1118-1134, 2017 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-29263586

RESUMO

The emergence and diffusion of green and sustainable technologies is full of obstacles and has therefore become an important area of research. We are interested in further understanding the dynamics between entrepreneurial experimentation, market formation, and institutional contexts, together playing a decisive role for successful diffusion of such technologies. Accordingly, we study these processes by adopting a technological innovation system perspective focusing on actors, networks, and institutions as well as the functions provided by them. Using a qualitative case study research design, we focus on the high-speed flywheel energy storage technology. As flywheels are based on a rotating mass allowing short-term storage of energy in kinetic form, they represent an environmentally-friendly alternative to electrochemical batteries and therefore can play an important role in sustainable energy transitions. Our contribution is threefold: First, regarding the flywheel energy storage technology, our findings reveal two subsystems and related markets in which development took different courses. In the automotive sector, flywheels are developing well as a braking energy recovery technology under the influence of two motors of innovation. In the electricity sector, they are stagnating at the stage of demonstration projects because of two important system weaknesses that counteract demand for storage. Second, we contribute to the theory of technological innovation systems by better understanding the internal dynamics between different functions of an innovation system as well as between the innovation system and its (external) contextual structures. Our third contribution is methodological. According to our best knowledge, we are the first to use system dynamics to (qualitatively) analyze and visualize dynamics between the diverse functions of innovation systems with the aim of enabling a better understanding of complex and iterative system processes. The paper also derives important implications for energy scholars, flywheel practitioners, and policymakers.

7.
Sci Justice ; 54(1): 89-94, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24438783

RESUMO

Sexual offences are under-reported and ascertaining accurate offence numbers is difficult. Any methods which could increase the ability to obtain biological evidence or reduce the additional distress associated with reporting a sexual offence may result in an increase in reporting this crime type. The Evidence Recovery System (ERS) is designed to collect trace evidence, including hairs, fibres and biological evidence, from bath or shower water in a non-invasive manner. Initially, samples of semen were placed in baths filled with water, and washing was simulated using a range of body wash products. The water was then drained through the ERS before its filters were subjected to acid phosphatase testing and haematoxylin and eosin staining of spermatozoa. Recovered spermatozoa were then graded accordingly. Following this, the experiment was repeated with the addition of dirt/dust particulates during the washing stage, to simulate recovery of biological evidence in a more realistic environment. The results showed that spermatozoa considered 'easy to find' could regularly be obtained from bathwater using the ERS. It appeared that this recovery was not affected by the presence of different body wash products. When dust/dirt particles were added, the number of spermatozoa recovered increased at two of the evidence collection stages. The difference in recovery was considered to be statistically significant. This study provides evidence to suggest the feasibility of use of the ERS as a method to collect semen evidence from individuals subjected to sexual offences. The recovery of spermatozoa does not appear to be affected by the presence of a body wash, but does appear to be improved when skin cells, hair and other debris are transferred into the water, as would be likely during a bath/shower. Further to this, the possibility of obtaining spermatozoa from the home bath or shower of a victim following a post-offence bathing experience is implied.


Assuntos
Banhos , Ciências Forenses/instrumentação , Sêmen/citologia , Espermatozoides/citologia , Água/química , Fosfatase Ácida , Humanos , Masculino , Coloração e Rotulagem
8.
Artigo em Inglês | MEDLINE | ID: mdl-39240429

RESUMO

This review paper provides a comprehensive examination of energy harvesting technologies tailored for electric vehicles (EVs). Against the backdrop of the automotive industry's rapid evolution towards electrification and sustainability, the paper explores a diverse range of techniques. The analysis encompasses the strengths, weaknesses, applicability in various scenarios, and potential implications for the future of EVs. A key finding of the review highlights regenerative braking as a pivotal and highly efficient method for energy recovery, particularly in urban settings. In addition to extending battery life, regenerative braking significantly boosts energy efficiency of EVs. The paper also delves into the challenges associated with integrated solar energy systems, emphasizing issues related to efficiency and weather dependency. Kinetic energy recovery systems (KERS) are discussed for their substantial power boost during acceleration in both motorsports and road cars. Additionally, the review explores regenerative shock absorbers, which capture energy from suspension movement, enhancing ride comfort and increasing vehicle energy economy, especially on uneven terrain. The piezoelectric system, though intriguing, is found to have low power output from mechanical vibration, prompting further exploration for integration into EVs. However, complexities and cost considerations arise in their integration with the vehicle's suspension system.

9.
Environ Sci Pollut Res Int ; 31(37): 49780-49799, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39083178

RESUMO

The paper aims to investigate the fuel and system options for a floating power plant (FPP) considering economic performance and the decarbonization goals of the International Maritime Organization. Various case studies have been assessed using a reference FPP, encompassing the instant and future retrofitting scenarios. The ready-to-use scenarios involve alternative fuel and organic Rankine cycle-based waste heat recovery system usage. Nuclear energy systems have been evaluated within the reference FPP since they are suitable candidates for achieving zero-carbon objectives and providing low-cost electricity. A simulation framework created in Python has calculated the fuel consumption regarding the power requirement and organized the approaches used in the study. An environmental model comparing the systems has been built to calculate upstream and operational emissions. The cost projection model for 2030 and 2050 has assessed the economic performance. Technique for Order of Preference by Similarity (TOPSIS) one of the multi-criteria decision-making approaches has ranked the systems considering the outcomes of economic and environmental models over the years. Findings demonstrate that the current fuel usage scenario of the FPP is not suitable both environmentally and economically. The other emissions can be near zero and greenhouse gases can be decreased by up to 15.95% using alternative fuels. Nuclear energy is a strong candidate to meet the 2050 targets, but its viability is largely based on economic performance.


Assuntos
Centrais Elétricas , Energia Nuclear
10.
Environ Technol ; : 1-9, 2023 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-37970915

RESUMO

Polyhydroxyalkanoates(PHAs) are considered a good alternative to petroleum-based plastics because of their good biodegradability and biocompatibility. The synthesis of PHAs using activated sludge can not only solve the problem of the high cost of pure cultures but also improve the utilization value of activated sludge. In this study, sludge activity recovery experiments were firstly conducted and the effects of different initial sludge concentrations on the activated sludge PHAs synthesis system were further investigated. the initial sludge concentrations were 1#SBR (2800 ± 50) mg/L, 2#SBR (4200 ± 50) mg/L, and 3#SBR (5500 ± 50) mg/L. The results showed that the activity, sedimentation performance and PHAs synthesis capacity of activated sludge were enhanced after the sludge activity recovery experiment. At the initial sludge concentration of 4200 mg/L, the activated sludge PHAs synthesis system was operated stably and the synthesis efficiency of PHAs was enhanced. In contrast, at the initial sludge concentration of 2800 and 5500 mg/L, the steady state of the activated sludge PHAs synthesis system was damaged to different degrees at different times, and the synthesis efficiency of PHAs was greatly reduced.

11.
F1000Res ; 12: 947, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-39464511

RESUMO

Past research aimed at service recovery has focused on actions that are taken to retain customers, and the possibility of overcoming the mistakes of service delivery, though the multidimensional nature of the service recovery process has begun to move beyond the complaint handling process. In this paper, we identify the most important issues addressed in service recovery and present a framework for addressing them. We used an extensive integrative review methodology. Between 1988 and 2017 the number of articles in these journals was kept between 26 and 30. The majority took a business perspective to study services recovery, while a minority took an inter-disciplinarity perspective. The study's findings are expected to provide insights into the antecedents and consequences of service recovery performance, particularly how job resources and demands influence employee burnout and work engagement, which in turn affect service recovery performance and customer perceptions of justice. The research aims to contribute to the discourse on service recovery by building a multi-level model that explains the service recovery performance of frontline employees and the impact of the service recovery system on customer justice perceptions. Thus, this research is expected to contribute to robust conclusions on the antecedents and consequences of employee service recovery performance nested within the unit-level construct, i.e., the service recovery system.


Assuntos
Justiça Social , Humanos , Comportamento do Consumidor , Satisfação no Emprego
12.
Membranes (Basel) ; 13(6)2023 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-37367782

RESUMO

The interest of the fusion community in Pd-Ag membranes has grown in the last decades due to the high value of hydrogen permeability and the possibility of continuous operation, making it a promising technology when a gaseous stream of hydrogen isotopes must be recovered and separated from other impurities. This is the case of the Tritium Conditioning System (TCS) of the European fusion power plant demonstrator, called DEMO. This paper presents an experimental and numerical activity aimed at (i) assessing the Pd-Ag permeator performance under TCS-relevant conditions, (ii) validating a numerical tool for scale-up purposes, and (iii) carrying out a preliminary design of a TCS based on Pd-Ag membranes. Experiments were performed by feeding the membrane with a He-H2 gas mixture in a specific feed flow rate ranging from 85.4 to 427.2 mol h-1 m-2. A satisfactory agreement between experiments and simulations was obtained over a wide range of compositions, showing a root mean squared relative error of 2.3%. The experiments also recognized the Pd-Ag permeator as a promising technology for the DEMO TCS under the identified conditions. The scale-up procedure ended with a preliminary sizing of the system, relying on multi-tube permeators with an overall number ranging between 150 and 80 membranes in lengths of 500 and 1000 mm each.

13.
ISA Trans ; 128(Pt B): 391-401, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34865843

RESUMO

The mathematical modeling and the control problem of the paraglider recovery system (PRS) are investigated in this paper. A 9-degree-of-freedom dynamic model describing the paraglider recovery control system is modeled including the inside relative rotation, the apparent mass, and the payload's time-varying inertia. On the basis of the attitude tracking error system, a novel practical fixed-time attitude tracking control approach is then proposed. The attitude tracking error is governed to converge into the small regions of the origin with a fixed-time convergence rate. Moreover, this rate is independent of any initial states. Simulation results are finally presented to illustrate the PRS performance obtained from the proposed control scheme.

14.
Ann Oper Res ; : 1-45, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-35075317

RESUMO

Product Recovery System (PRS) transfers products from their typical final place to their source to arrest some value on the product. There are obstructions, such as costs, associated with the modification of accounts and assessment of products and refunds associated with the implementation of PRS. Blockchain Technology (BCT) emerged as an innovative approach to constructing trust in a trust less environment and assures the availability, traceability, and security in data management. It also presents a valuable solution to PRS. This study aims to analyze the Blockchain Readiness Challenges (BRCs) to PRS in the context of manufacturing industries. The study observes 20 readiness challenges linked with the implementation of BCT in PRS. The BRCs are identified from the literature survey and confirmed after consequent examinations with industry experts and researchers. The study employed a Multi-Criteria Decision-Making (MCDM) i.e., the Decision-Making Trial And Evaluation Laboratory (Fuzzy DEMATEL) approach to find the cause-and-effect interactions to prioritize BRCs. The Maximum Mean De-Entropy (MMDE) algorithm was adopted to establish the threshold value based on the information entropy of the interactions among the BRCs for PRS. The fuzzy set theory was adopted to tackle the uncertainty and vagueness of personnel biases and data deficiency problems. The findings from this study reveal that inadequate financing for PRS exercises, lack of governance and standards, and security challenges to BCT implementation are the most influential readiness challenges for the adoption of blockchain in PRS. The study is useful to manufacturing organizations for identifying the potential BRCs to implement PRS among all existing readiness challenges so that they can take suitable measures before proceeding to adopt blockchain in PRS. The managers are suggested to eliminate the readiness challenges and widen the blockchain technology adoption in PRS.

15.
ISA Trans ; 130: 136-151, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35523606

RESUMO

This paper is concerned with the homing process in which a multi-underactuated AUV recovery system tracks the moving mother submarine in finite time in the presence of uncertain hydrodynamic parameters and unknown environmental disturbances. In the homing stage, underactuated AUVs and the moving mother submarine are treated as followers and the leader, respectively. The multi-underactuated AUV system finite time homing problem is converted to the leader-following finite-time formation control problem. The proposed leader-following formation strategy requires only the position information of the leader. The velocity of the leader can be designed as an additional degrees of freedom to stabilize position errors of the formation. A novel robust adaptive super-twisting sliding mode formation controller (ASTASMC) is proposed, specifically the super-twisting algorithm (STA) with adaptive uncertainty estimation. Wherein, the robust adaptive law can compensate for uncertainty with unknown upper bound in real time. Therefore, the proposed controller can not only enhance the tracking accuracy but also reduce the chattering. In addition, the finite-time convergence of estimation errors and tracking errors is rigorously proved. Finally, simulation results demonstrate the effectiveness of theoretical results.

16.
Membranes (Basel) ; 11(2)2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33671254

RESUMO

For providing advanced desalination the combination of the improvement of water recovery ratio in the reverse osmosis (RO) process and the No-Chlorine/No-Sodium Bisulfite (SBS) Dosing process was studied. In order to prevent membrane fouling even in high recovery water operations, an advanced two-stage design was implemented to (1) control the permeate flux through the RO membrane module, (2) optimize the system to reduce contaminant build-up and (3) eliminate the use of chlorine and SBS, which can accelerate membrane fouling. The system was evaluated by monitoring the biofouling and the microorganisms proliferation on the membrane surface based on membrane biofilm formation rate (mBFR). The pilot plant was operated in the condition of a water recovery rate of 55%. As a result, the system was operated for longer than four months without membrane cleaning (clean in place; CIP) and the possibility of operation for seven months without CIP was confirmed by the extrapolation of the pressure values. In addition, the mBFR is a reliable tool for water quality assessment, based on a comparison between the fouling tendency estimated from the mBFR and the actual membrane surface condition from autopsy study and the effectiveness No-Chlorine/No-SBS Dosing process was verified from mBFR of pretreated seawater.

17.
Bioresour Technol ; 332: 125077, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33823475

RESUMO

The microbial nutrient recovery cell i.e. modified microbial fuel cell containing a middle recovery chamber can be used to purify wastewater and remove valuable nutrients, while simultaneously generating electricity. The study investigated nutrient removal and microorganism interactions with carbon (CB- HT and CB- APTES) and stainless steel (SSB-HT) modified anodes used in microbial nutrient recovery cells. The removal efficiencies of ammonium ions were found higher in carbon-based CB-APTES (~98%) and CB-HT (~98.27%) systems in comparison to SSB-HT (~87.16%) system. On comparing further, the removal efficiencies of chemical oxygen demand (~99.5%) and total phosphorus (~99%) in CB- APTES system were superior to the cases of CB- HT, and SSB- HT systems. Besides, the CB-APTES based microbial fuel cell (MFC) displayed an average stable voltage of 0.5 V and a maximum power density of ~ 850 mW/m2.


Assuntos
Fontes de Energia Bioelétrica , Eletricidade , Eletrodos , Nutrientes , Águas Residuárias
18.
Appl Radiat Isot ; 147: 48-53, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30798205

RESUMO

Cd isotopes (107Cd and 109Cd) are generated from the silver target body during the bombardment of [18O]water in the routine production of 2-[18F]fluoro-2-deoxy-d-glucose ([18F]FDG) for PET (Positron Emission Tomography) diagnosis. Cadmium isotopes contribute significantly to the total activity of generated impurities and, due to being potentially radiotoxic to living organisms, they should be effectively reduced from FDG to prevent accidental injection of even small concentrations into patients. Purification of the final [18F]FDG can be based on a set of columns, fulfilling various functions in the cleaning process. To assess cadmium impurities and the efficiency of the purification process, a low background gamma spectrometry system with high resolution has been applied. Even activity of 3.5 kBq and 290 kBq has been measured on QMA (Sep-Pak Light Accell Plus QMA) columns for 109Cd and 107Cd isotopes, respectively. 107Cd activity in the five column set was higher than that of 109Cd. The rate of 18F production process was about 1 GBq/min, while that of 107Cd and 109Cd radionuclides was about 4.2 kBq/min and 50 Bq/min respectively. The same purification efficiency of both isotopes has been obtained at each step of the process. The production rate of 107Cd and 109Cd radionuclides was insignificant compared to the 18F production rate. Therefore [18F]FDG final product for use in injections before PET diagnostics was efficiently purified from cadmium radionuclide impurities.


Assuntos
Radioisótopos de Cádmio/química , Radioisótopos de Flúor/química , Humanos , Tomografia por Emissão de Pósitrons/métodos , Padrões de Referência
19.
East Mediterr Health J ; 25(2): 104-110, 2019 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-30942474

RESUMO

BACKGROUND: As blood is a scarce and expensive resource, irrational blood usage places huge burden on health expenditures. In response to this challenge, governments and health care providers are developing different strategies to optimize blood utilization. Among these strategies is trying to raise the public awareness on the actual costs of the blood production and changing the cost recovery systems of blood and blood components. AIMS: This study aims to compare cost recovery and financing systems of blood and blood products in different countries. METHODS: This research was an email-based survey of 30 countries from four HDI categories. All related literature was reviewed. RESULTS: Out of 28 countries, 19 have blood and blood products that are provided totally free of charge to the patients. In nine countries blood and blood products are totally or partially chargeable to the patients. CONCLUSIONS: In countries with low and lower-middle income economies, total or partial costs of blood and blood products are recovered directly from the patients. While countries in which blood and blood products are 'free of charge' for patients are mostly categorized in upper-middle- or high-income economies with well-developed healthcare and insurance systems. There is no clear relation between blood usage and the type of cost recovery system. However, having an efficient cost recovery system will help blood establishments to sustain their service delivery.


Assuntos
Transfusão de Sangue/economia , Sangue , Honorários Médicos , Financiamento da Assistência à Saúde , Análise Custo-Benefício , Gastos em Saúde/estatística & dados numéricos , Humanos , Cobertura do Seguro/economia , Cobertura do Seguro/estatística & dados numéricos , Inquéritos e Questionários
20.
F1000Res ; 8: 348, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31031972

RESUMO

Plant seeds are essential for human beings, constituting 70% of carbohydrate resources worldwide; examples include rice, wheat, and corn. In angiosperms, fertilization of the egg by a sperm cell is required for seed formation; therefore, fertilization failure results in no seed formation, except in the special case of apomixis. Initially, plants produce many pollen grains inside the anthers; once the pollen grain is deposited onto the top of the pistil, the pollen tube elongates until it reaches the ovule. Generally, only one pollen tube is inserted into the ovule; however, we previously found that if fertilization by the first pollen tube fails, a second pollen tube could rescue fertilization via the so-called fertilization recovery system (FRS). Our previous reports also demonstrated that failed fertilization results in pollen tube-dependent ovule enlargement morphology (POEM), enlarged seeds, and partial seed coat formation if the pollen tube releases the pollen tube contents into the ovule. However, we have not determined whether all the ovules enlarge or produce seed coats if an ovule accepts the pollen tube contents. Therefore, we conducted a partial seed coat formation experiment taking into account both the FRS and POEM phenomena. Notably, the ratios of failed fertilization and the ovules with partial seed coats matched, indicating that all ovules initiate seed coat formation if the fertilization fails but the pollen tube contents enter the ovule. In addition, we confirmed that the agl62 mutant , defective in early endosperm formation, showed seed coat initiation with and without fertilization, indicating that for a normal seed coat initiation, fertilization is not required; however, for the completion of normal seed coat formation, both normal fertilization and endosperm formation are required. Further molecular evidence is required to understand these phenomena because very few factors related to FRS and POEM have been identified.


Assuntos
Arabidopsis , Tubo Polínico , Sementes , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Mutação , Óvulo Vegetal
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