RESUMO
Ozonated sunflower oil (OSO) is an established therapeutic agent and nutraceutical harboring various therapeutic values, including antiallergic, derma-protective, and broad-spectrum antimicrobial activity. Conversely, the medicinal aspects of OSO for wound healing, tissue regeneration, and treatment of inflammation in dyslipidemia have yet to be fully elucidated. Herein, a comparative effect of OSO and sunflower oil (SO) was investigated to heal cutaneous wound and tissue regeneration of zebrafish impediment by carboxymethyllysine (CML) toxicity, following impact on hepatic inflammation and blood lipid profile. After OSO (final 2%, 1 µL) and SO's (final 2%, 1 µL) treatment, substantial healing was elicited by OSO in the cutaneous wound of zebrafish impaired by CML (final 25 µg). As an important event of wound healing, OSO scavenges the reactive oxygen species (ROS), rescues the wound from oxidative injury, and triggers the essential molecular events for the wound closer. Furthermore, the intraperitoneal injection of OSO was noted to counter the CML-induced adversity and prompt tissue regeneration in the amputated tail fin of zebrafish. Additionally, OSO counters the CML-induced neurotoxicity and rescues the zebrafish from acute mortality and paralysis, along with meticulous recovery of hepatic inflammation, fatty liver changes, and diminished ROS and proinflammatory interleukin (IL)-6 production. Moreover, OSO efficiently ameliorated CML-induced dyslipidemia by alleviating the total blood cholesterol (TC), triglyceride (TG), and increasing high-density lipoproteins cholesterol (HDL-C). The outcome of multivariate assessment employing principal component analysis and hierarchical cluster analysis supports a superior therapeutic potential of OSO over SO against the clinical manifestation of CML. Conclusively, OSO owing to its antioxidant and anti-inflammatory potential, counters CML-induced toxicity and promotes wound healing, tissue regeneration, hepatoprotection, improved blood lipid profile, and survivability of zebrafish.
RESUMO
A periodontite é uma patologia multifatorial, que apresenta alta prevalência. Caracteriza-se pela destruição dos tecidos periodontais de suporte, por ocasionar a reabsorção óssea e culminar com a perda dentária. Modificadores da resposta cicatricial, tais como os fatores de crescimento, têm sido aplicados no tratamento das sequelas da doença, na tentativa de se obter a regeneração dos tecidos periodontais e peri-implantares perdidos. Pesquisas recentes, em animais e humanos, têm mostrado resultados promissores com o uso do fator de crescimento derivado de plaquetas e da proteína morfogenética óssea para o tratamento de defeitos ósseos, lesões de bifurcações e para auxiliar reabilitações com implantes dentários. Estes resultados permitiram o desenvolvimento e lançamento de dois produtos comerciais. Esta revisão da literatura tem o objetivo de comentar criticamente as possibilidades clínicas e os resultados do uso destes dois produtos
Periodontitis is a multifactorial disease, with high prevalence. It is characterized by destruction of periodontal tissues, leading to bone resorption and to tooth loss. Modifiers of the healing response such as growth factors, has been applied in the treatment of sequelae of the disease to achieve the regeneration of lost periodontal and peri-implant tissues. Recent research in animals and humans have shown promising results with the use of growth factor derived from platelets and bone morphogenetic protein for the treatment of bone defects, lesions in bifurcations and to assist rehabilitations with dental implants. These results enabled the development and launch of two commercial products. Therefore this review aims to critically comment on the scope and results of clinical use of these products.