Short-term acclimation dynamics in a coldwater fish.

Critical thermal maximum (CTmax) is widely used for measuring thermal tolerance but the strong effect of acclimation on CTmax is a likely source of variation within and among studies/species that makes comparisons more difficult. There have been surprisingly few studies focused on quantifying how quickly acclimation occurs or that combine temperature and duration effects. We studied the effects of absolute temperature difference and duration of acclimation on CTmax of brook trout (Salvelinus fontinalis), a well-studied species in the thermal biology literature, under laboratory conditions to determine how each of the two factors and their combined effects influence critical thermal maximum. Using an ecologically-relevant range of temperatures and testing CTmax multiple times between one and 30 days, we found that both temperature and duration of acclimation had strong effects on CTmax. As predicted, fish that were exposed to warmer temperatures longer had increased CTmax, but full acclimation (i.e., a plateau in CTmax) did not occur by day 30. Therefore, our study provides useful context for thermal biologists by demonstrating that the CTmax of fish can continue to acclimate to a new temperature for at least 30 days. We recommend that this be considered in future studies measuring thermal tolerance that intend to have their organisms fully acclimated to a given temperature. Our results also support using detailed thermal acclimation information to reduce uncertainty caused by local or seasonal acclimation effects and to improve the use of CTmax data for fundamental research and conservation planning.

Rapid cardiac thermal acclimation in wild anadromous Arctic char (Salvelinus alpinus).

Migratory fishes commonly encounter large and rapid thermal variation, which has the potential to disrupt essential physiological functions. Thus, we acclimated wild, migratory Arctic char to 13°C (∼7°C above a summer average) for an ecologically relevant period (3 days) and measured maximum heart rate (ƒH,max) during acute warming to determine their ability to rapidly improve cardiac function at high temperatures. Arctic char exhibited rapid compensatory cardiac plasticity similar to past observations following prolonged warm acclimation: they reduced ƒH,max over intermediate temperatures (-8%), improved their ability to increase ƒH,max during warming (+10%), and increased (+1.3°C) the temperature at the onset of an arrhythmic heartbeat, a sign of cardiac failure. This rapid cardiac plasticity may help migrating fishes such as Arctic char mitigate short-term thermal challenges. Furthermore, by using mobile Arctic research infrastructure in a remote field location, the present study illustrates the potential for field-based, experimental physiology in such locations.

Impact of low temperatures on the immune system of honeybees.

Changes in temperature resulting from climate change can impact the distribution and survival of species, including bees, where temperature may also affect their immune system. Evaluation of immune system activity is often performed by the total count of circulating hemocytes in the hemolymph. However, there are few studies on bees examining the relationship between the amount of circulating hemocytes and temperature. This study evaluated changes of circulating hemocytes in Apis mellifera hemolymph at different temperatures and development stages. Total hemocytes of bees were determined at - 8, 16, 24, and 32 °C - and at different development stages - in vivo larvae, in vitro larvae, newly emerged, and forager bees. A. mellifera larvae had a greater number of circulating hemocytes compared to the other development stages (newly emerged and foragers). Additionally, temperature was an important factor explaining variation of circulating hemocytes in the hemolymph, according to principal component analyses (PCA), as the number of circulating hemocytes was greater at higher temperatures. Therefore, extreme events arising from climate change, such as variation in temperature, can directly impact the immune system of bees, both individually and at the colony level, threatening the distribution and survival of several species.

The temperature dependence of gradient system response characteristics.

PURPOSE: The gradient system transfer function (GSTF) characterizes the frequency transfer behavior of a dynamic gradient system and can be used to correct non-Cartesian k-space trajectories. This study analyzes the impact of the gradient coil temperature of a 3T scanner on the GSTF. METHODS: GSTF self- and B0 -cross-terms were acquired for a 3T Siemens scanner (Siemens Healthcare, Erlangen, Germany) using a phantom-based measurement technique. The GSTF terms were measured for various temperature states up to 45°C. The gradient coil temperatures were measured continuously utilizing 12 temperature sensors which are integrated by the vendor. Different modeling approaches were applied and compared. RESULTS: The self-terms depend linearly on temperature, whereas the B0 -cross-term does not. Effects induced by thermal variation are negligible for the phase response. The self-terms are best represented by a linear model including the three gradient coil sensors that showed the maximum temperature dependence for the three axes. The use of time derivatives of the temperature did not lead to an improvement of the model. The B0 -cross-terms can be modeled by a convolution model which considers coil-specific heat transportation. CONCLUSION: The temperature dependency of the GSTF was analyzed for a 3T Siemens scanner. The self- and B0 -cross-terms can be modeled using a linear and convolution modeling approach based on the three main temperature sensor elements.

Effects of aerial hypoxia and temperature on pulmonary breathing pattern and gas exchange in the South American lungfish, Lepidosiren paradoxa.

The South American lungfish Lepidosiren paradoxa is an obligatory air-breathing fish possessing well-developed bilateral lungs, and undergoing seasonal changes in its habitat, including temperature changes. In the present study we aimed to evaluate gas exchange and pulmonary breathing pattern in L. paradoxa at different temperatures (25 and 30°C) and different inspired O2 levels (21, 12, 10, and 7%). Normoxic breathing pattern consisted of isolated ventilatory cycles composed of an expiration followed by 2.4±0.2 buccal inspirations. Both expiratory and inspiratory tidal volumes reached a maximum of about 35mlkg-1, indicating that L. paradoxa is able to exchange nearly all of its lung air in a single ventilatory cycle. At both temperatures, hypoxia caused a significant increase in pulmonary ventilation (V̇E), mainly due to an increase in respiratory frequency. Durations of the ventilatory cycle and expiratory and inspiratory tidal volumes were not significantly affected by hypoxia. Expiratory time (but not inspiratory) was significantly shorter at 30°C and at all O2 levels. While a small change in oxygen consumption (V̇O2) could be noticed, the carbon dioxide release (V̇CO2, P=0.0003) and air convection requirement (V̇E/V̇O2, P=0.0001) were significantly affected by hypoxia (7% O2) at both temperatures, when compared to normoxia, and pulmonary diffusion capacity increased about four-fold due to hypoxic exposure. These data highlight important features of the respiratory system of L. paradoxa, capable of matching O2 demand and supply under different environmental change, as well as help to understand the evolution of air breathing in lungfish.

Ecological differences influence the thermal sensitivity of swimming performance in two co-occurring mysid shrimp species with climate change implications.

Temperature strongly affects performance in ectotherms. As ocean warming continues, performance of marine species will be impacted. Many studies have focused on how warming will impact physiology, life history, and behavior, but few studies have investigated how ecological and behavioral traits of organisms will affect their response to changing thermal environments. Here, we assessed the thermal tolerances and thermal sensitivity of swimming performance of two sympatric mysid shrimp species of the Northwest Atlantic. Neomysis americana and Heteromysis formosa overlap in habitat and many aspects of their ecological niche, but only N. americana exhibits vertical migration. In temperate coastal ecosystems, temperature stratification of the water column exposes vertical migrators to a wider range of temperatures on a daily basis. We found that N. americana had a significantly lower critical thermal minimum (CTmin) and critical thermal maximum (CTmax). However, both mysid species had a buffer of at least 4°C between their CTmax and the 100-year projection for mean summer water temperatures of 28°C. Swimming performance of the vertically migrating species was more sensitive to temperature variation, and this species exhibited faster burst swimming speeds. The generalist performance curve of H. formosa and specialist curve of N. americana are consistent with predictions based on the exposure of each species to temperature variation such that higher within-generation variability promotes specialization. However, these species violate the assumption of the specialist-generalist tradeoff in that the area under their performance curves is not constant. Our results highlight the importance of incorporating species-specific responses to temperature based on the ecology and behavior of organisms into climate change prediction models.

Thermal variation and factors influencing vertical migration behavior in Daphnia populations.

The antipredator behavior diel vertical migration (DVM), common in aquatic keystone species Daphnia, involves daily migration from warmer surface waters before dawn to cooler deeper waters after dusk. Plasticity in Daphnia DVM behavior optimizes fitness via trade-offs between growth, reproduction, and predator avoidance. Migration behavior is affected by co-varying biotic and abiotic factors, including light, predator cues, and anthropogenic stressors making it difficult to determine each factor's individual contribution to the variation in this behavior. This study aims to better understand this ecologically significant behavior in Daphnia by: (1) determining how Daphnia pulicaria thermal preferences vary within and among natural populations; (2) distinguishing the role of temperature verses depth in Daphnia vertical migration; and (3) defining how two anthropogenic stressors (copper and nickel) impact Daphnia migratory behavior. Simulated natural lake stratification were constructed in 8L (0.5m tall, 14.5cm wide) water columns to monitor under controlled laboratory conditions the individual effects of temperature gradients, depth, and metal stressors on Daphnia vertical migration. Three major findings are reported. First, while no difference in thermal preference was found among the four populations studied, within lake populations variability among isolates was high. Second, decoupling temperature and depth revealed that depth was a better predictor of Daphnia migratory patterns over temperature. Third, exposure to environmentally relevant concentrations of copper or nickel inhibited classic DVM behavior. These findings revealed the high variability in thermal preference found within Daphnia populations, elucidated the individual roles that depth and temperature have on migratory behavior, and showed how copper and nickel can interfere with the natural response of Daphnia to fish predator cues. Thus contributing to the body of knowledge necessary to predict how natural populations of Daphnia will be affected by climate related changes in lake temperatures and increased presence of anthropogenic stressors.

Thermal variation in human temporal bone using rigid endoscope.

OBJECTIVE: Compare thermal variation in the region of the External Acoustic Canal (EAC) and the Round Window (RW) using different rigid endoscopes and light sources in human temporal bones. METHOD: This is an analytical experimental study using human temporal bones. Thermal variation was assessed during ten minutes, using a thermometer in the region of the EAC and the RW of two temporal bones, right and left. We used three different endoscopes (0° 4-mm, 0° 3-mm and 30° 4-mm) and five intensity/type light source (Halogen 100%, LED 50%, LED 100%, Xenon 50% and Xenon 100% with the same endoscope light fiber. RESULTS: We found temperature elevations in the EAC and RW in all measurements. Larger caliber endoscopes (4-mm) and light sources at 100% intensity generated higher temperatures, slightly higher in halogen and xenon. The 30° endoscopes tended to put more heat on structures, with little difference in most measurements. We identified greater temperature variations in the EAC of the right and left temporal bones compared to the RW overall. The highest temperature acquired in the present study was in the EAC of the temporal bone with a 4-mm and 30° endoscope, using xenon light source (intensity of 100%), with an increase of 4.51 °C. CONCLUSION: The type of endoscope and light source can influence the thermal variation and the risk of tissue injury during endoscopic ear surgery. Larger endoscopes with xenon and halogen light sources at maximum intensity generate more heat.

Warming and top-down control of stage-structured prey: Linking theory to patterns in natural systems.

Warming has broad and often nonlinear impacts on organismal physiology and traits, allowing it to impact species interactions like predation through a variety of pathways that may be difficult to predict. Predictions are commonly based on short-term experiments and models, and these studies often yield conflicting results depending on the environmental context, spatiotemporal scale, and the predator and prey species considered. Thus, the accuracy of predicted changes in interaction strength, and their importance to the broader ecosystems they take place in, remain unclear. Here, we attempted to link one such set of predictions generated using theory, modeling, and controlled experiments to patterns in the natural abundance of prey across a broad thermal gradient. To do so, we first predicted how warming would impact a stage-structured predator-prey interaction in riverine rock pools between Pantala spp. dragonfly nymph predators and Aedes atropalpus mosquito larval prey. We then described temperature variation across a set of hundreds of riverine rock pools (n = 775) and leveraged this natural gradient to look for evidence for or against our model's predictions. Our model's predictions suggested that warming should weaken predator control of mosquito larval prey by accelerating their development and shrinking the window of time during which aquatic dragonfly nymphs could consume them. This was consistent with data collected in rock pool ecosystems, where the negative effects of dragonfly nymph predators on mosquito larval abundance were weaker in warmer pools. Our findings provide additional evidence to substantiate our model-derived predictions while emphasizing the importance of assessing similar predictions using natural gradients of temperature whenever possible.

Thermal plasticity and sensitivity to insecticides in populations of an invasive beetle: Cyfluthrin increases vulnerability to extreme temperature.

Climate change increases average temperatures and the occurrence of extreme weather events, in turn accentuating the risk of organism exposure to temperature stress. When thermal conditions become stressful, the sensitivity of insects toward insecticides can be exacerbated. Likewise, exposure of insects to insecticides can subsequently influence their ability to handle stressful temperatures. Here, we investigated the effects of constant temperature and daily heat spikes, in presence/absence of insecticide treatment (cyfluthrin), on the condition (impairment of mobility) and thermal tolerance to cold (-6 °C) and heat (42.5 °C) of the terrestrial beetle Alphitobius diaperinus. The responses of insects from four populations (three farm-collected populations, one laboratory population) to different durations of extreme temperature exposure were compared. The results showed that the laboratory population was generally more sensitive to extreme cold and heat temperatures, with less than 50% of adults recovering after an exposure at -6 or +42.5 °C for 3h. Significant differences in the level of thermal tolerance were also found among insects from poultry farms. Cyfluthrin exposure incurred detrimental effects to insects' condition in all but one population. For two out of the four populations, mobility impairment was increased when adults were exposed to daily heat spikes (6 h per day at 38 °C) and cyfluthrin simultaneously, compared to cyfluthrin exposure at constant temperatures; yet, no significant interaction between the two stressors was found. Finally, using one farm collected population, effects of pre-exposure to cyfluthrin on extreme temperature tolerance provided another example of the toxicant-induced climate sensitivity in insects.

Thermal tolerance and routine oxygen consumption of convict cichlid, Archocentrus nigrofasciatus, acclimated to constant temperatures (20 °C and 30 °C) and a daily temperature cycle (20 °C → 30 °C).

Organismal temperature tolerance and metabolic responses are correlated to recent thermal history, but responses to thermal variability are less frequently assessed. There is great interest in whether organisms that experience greater thermal variability can gain metabolic or tolerance advantages through phenotypic plasticity. We compared thermal tolerance and routine aerobic metabolism of Convict cichlid acclimated for 2 weeks to constant 20 °C, constant 30 °C, or a daily cycle of 20 → 30 °C (1.7 °C/h). Acute routine mass-specific oxygen consumption ([Formula: see text]O2) and critical thermal maxima/minima (CTMax/CTMin) were compared between groups, with cycle-acclimated fish sampled from the daily minimum (20 °C, 0900 h) and maximum (30 °C, 1600 h). Cycle-acclimated fish demonstrated statistically similar CTMax at the daily minimum and maximum (39.0 °C, 38.6 °C) but distinct CTMin values, with CTMin 2.4 °C higher for fish sampled from the daily 30 °C maximum (14.8 °C) compared to the daily 20 °C minimum (12.4 °C). Measured acutely at 30 °C, [Formula: see text]O2 decreased with increasing acclimation temperature; 20 °C acclimated fish had an 85% higher average [Formula: see text]O2 than 30 °C acclimated fish. Similarly, acute [Formula: see text]O2 at 20 °C was 139% higher in 20 °C acclimated fish compared to 30 °C acclimated fish. Chronic [Formula: see text]O2 was measured in separate fish continually across the 20 → 30 °C daily cycle for all 3 acclimation groups. Chronic [Formula: see text]O2 responses were very similar between groups between average individual hourly values, as temperatures increased or decreased (1.7 °C/h). Acute [Formula: see text]O2 and thermal tolerance responses highlight "classic" trends, but dynamic, chronic trials suggest acclimation history has little effect on the relative change in oxygen consumption during a thermal cycle. Our results strongly suggest that the minimum and maximum temperatures experienced more strongly influence fish physiology, rather than the thermal cycle itself. This research highlights the importance of collecting data in both cycling and static (constant) thermal conditions, and further research should seek to understand whether ectotherm metabolism does respond uniquely to fluctuating temperatures.

Short-Term Cold Stress Affects Parasitism on the Asian Chestnut Gall Wasp Dryocosmus kuriphilus.

Temperature variation affects interactions involving plants, herbivores, and parasitoids, causing a mismatch between their phenological cycles. In the context of climate change, climatic factors can undergo profound and sudden changes, such as sudden hot or cold snaps. Herein, we show that the number of episodes of short but sustained low temperatures has increased, mainly during May, over the last two decades. We subjected galls induced by the Asian chestnut gall wasp (ACGW) Dryocosmus kuriphilus to cold stress to assess whether and, if so, how it affected the pest and its parasitoids. Over the course of two years, we measured seasonal parasitism, parasitism rates, the relative abundance of each parasitoid species, and ACGW mortality. We found that the cold treatment affected both the pest and the parasitoids, resulting in a reduction in the emergence of ACGWs and differing ratios of species within the parasitoid community. The most striking example was the change in the relative frequency of three species of Eupelmus spp. and Mesopolobus tibialis, which doubled in cold-stressed galls in all chestnut fields. The effects of temperature on the development of the host and the direct effects of cold temperatures on the surface of galls (in terms of the humidity or hardness of the galls) warrant further research in this direction.

Thermal variation in human temporal bone using rigid endoscope

Abstract Objective Compare thermal variation in the region of the External Acoustic Canal (EAC) and the Round Window (RW) using different rigid endoscopes and light sources in human temporal bones. Method This is an analytical experimental study using human temporal bones. Thermal variation was assessed during ten minutes, using a thermometer in the region of the EAC and the RW of two temporal bones, right and left. We used three different endoscopes (0° 4-mm, 0° 3-mm and 30° 4-mm) and five intensity/type light source (Halogen 100%, LED 50%, LED 100%, Xenon 50% and Xenon 100% with the same endoscope light fiber. Results We found temperature elevations in the EAC and RW in all measurements. Larger caliber endoscopes (4-mm) and light sources at 100% intensity generated higher temperatures, slightly higher in halogen and xenon. The 30° endoscopes tended to put more heat on structures, with little difference in most measurements. We identified greater temperature variations in the EAC of the right and left temporal bones compared to the RW overall. The highest temperature acquired in the present study was in the EAC of the temporal bone with a 4-mm and 30° endoscope, using xenon light source (intensity of 100%), with an increase of 4.51 °C. Conclusion The type of endoscope and light source can influence the thermal variation and the risk of tissue injury during endoscopic ear surgery. Larger endoscopes with xenon and halogen light sources at maximum intensity generate more heat. Level of evidence: 5.

Influence of Temperature on the Longitudinal Cracking in Multipurpose Precast Concrete Sleepers Prior to Their Installation.

Prestressed monoblock railway sleepers are concrete elements with almost no reinforcement apart from the prestressing wires, which makes them very sensitive to any stress variation that can induce tensile stresses. In recent years, severe longitudinal cracking has been observed in a number of sleepers in hot regions of Spain, even before these elements were put in service. This work studies the problem while considering the thermal variation as the main factor affecting this cracking phenomenon. A non-linear static load-step analysis is applied on a non-linear finite element model to reproduce the problem and, after its experimental validation, the influence of three design parameters of the sleepers are studied: the nature of concrete aggregates, the dowel thickness, and the dowel material. The results show that all these three parameters may have significant influence on the problem, with the dowel material being the most important parameter. When the dowels are made of a material with a high elastic modulus and a high thermal expansion coefficient, the crack opening induced by a realistic thermal variation can reach significant values and result in longitudinal crack propagation. The changes of humidity are not considered in this study because they are beyond the scope of this work.

An intertidal fish shows thermal acclimation despite living in a rapidly fluctuating environment.

The co-evolution of acclimation capacity and thermal performance breadth has been a contentious issue for decades, and little is known regarding the extent to which acclimation alters the shape of acute thermal performance curves. Current acclimation theory suggests that when daily variation is large and unpredictable ectotherms should not acclimate but should evolve wide performance breadths, allowing maintenance of performance across a wide thermal range. The subtropical intertidal zone, however, experiences a large amount of daily thermal variation, but daily means and ranges shift in predictable ways with season, where daily and seasonal variation is roughly equal. We predicted that animals in this habitat would maintain their capacity to acclimate and that performance breadth would not be altered by acclimation to maintain function with rapidly fluctuating daily temperatures. We tested our prediction using a subtropical goby, Bathygobius cocosensis, which lives in tide pools that vary widely, over days and seasons. We exposed B. cocosensis to winter (12-17 °C) and summer (30-35 °C) thermal conditions for six weeks and then measured the thermal dependence of burst swimming speed, routine and maximum metabolic rate, and ventilation rate between 12 and 36 °C. B. cocosensis exhibited an acclimation response for burst swimming speed, maximum metabolic rate and metabolic scope, but acclimation did not alter the shape of acute thermal performance curves. These results indicate that thermal acclimation can occur when short-term thermal variability is large and equal to seasonal variation, and wide performance breadths can be maintained with acclimation in heterogeneous environments.

Distinct Responses of the Nitrogen-Fixing Marine Cyanobacterium Trichodesmium to a Thermally Variable Environment as a Function of Phosphorus Availability.

Surface temperature in the ocean is projected to be elevated and more variable in the future, which will interact with other environmental changes like reduced nutrient supplies. To explore these multiple stressor relationships, we tested the influence of thermal variation on the key marine diazotrophic cyanobacterium Trichodesmium erythraeum GBRTRLI101 as a function of the limiting nutrient phosphorus (P). Two constant temperature treatments represented current winter (22°C) and summer (30°C) mean values. Three variable temperature treatments fluctuated around the constant control values: Mean 22°C, either ± 2°C or ± 4°C; and mean 30°C ± 2°C. Each thermal treatment was grown under both P-replete (10 µmol/L) and P-limiting conditions (0.2 µmol/L). Effects of thermal variability on Trichodesmium were mainly found in the two winter variable temperature treatments (22°C ± 2°C or ± 4°C). P availability affected growth and physiology in all treatments and had significant interactions with temperature. P-replete cultures had higher growth and nitrogen and carbon fixation rates in the 22°C constant control, than in the corresponding variable treatments. However, physiological rates were not different in the P-replete constant and variable treatments at 30°C. In contrast, in P-limited cultures an advantage of constant temperature over variable temperature was not apparent. Phosphorus use efficiencies (PUE, mol N or C fixed h-1 mol cellular P-1) for nitrogen and carbon fixation were significantly elevated under P-limited conditions, and increased with temperature from 22 to 30°C, implying a potential advantage in a future warmer, P-limited environment. Taken together, these results imply that future increasing temperature and greater thermal variability could have significant feedback interactions with the projected intensification of P-limitation of marine N2-fixing cyanobacteria.

Indicadores de estabilidad aeróbica en ensilajes de la mezcla Tithonia diversifolia/Pennisetum purpureum enriquecidos con lactobacillus / Indicators of aerobic stability in silages of the mixture Tithonia diversifolia/Pennisetum purpureum enriched with lactobacillus

RESUMEN En épocas de escasez, los ganaderos recurren a conservar el alimento mediante procesos de fermentación anaeróbica, conocidos como ensilajes. Una vez que el silo es abierto, el sustrato resulta aeróbicamente estable, cuando, al momento de la apertura, conserva su integridad nutricional. Esto es el resultado de un ambiente ácido que restringe los efectos deletéreos de la acción de microrganismos patógenos, expresado en indicadores de estabilidad a la exposición aeróbica. Uno de los problemas empíricos que enfrenta el productor es resolver la pregunta de cómo evitar el deterioro del ensilaje, una vez es abierto, por lo cual, esta investigación apunta a la resistencia en el periodo de exposición aeróbica (PEA) de un ensilaje de Pennisetum purpureum (PP), con diferentes niveles de sustitución de Tithonia diversifolia (TD). El estudio, se adelantó en el Centro Internacional de Agricultura Tropical en Colombia. Con un ensilaje de 90 días de fermentación, se realizó una prueba de estabilidad aeróbica de siete días, usando ensilajes de un kilogramo, a diferentes proporciones (%) de TD/PP: 100/0, 67/33, 33/67 y 0/100, enriquecidos con dos aditivos basados en Lactobacillus. Se midió el cambio de la materia seca, temperatura y el pH, encontrándose una estabilidad a la exposición aeróbica. El papel de TD es notable, una vez que el ensilado entra en el PEA, debido a la capacidad tampón que amortigua el cambio de pH, una vez que se abre el silo, generando mayor estabilidad aeróbica.

ABSTRACT In times of scarcity, farmers resort to preserving food through anaerobic fermentation processes, known as silage. Once the silo is opened, the substrate is aerobically stable if it retains its nutritional integrity when opening. This is the result of an acidic environment that restricts the deleterious effects of the action of pathogenic microorganisms, expressed as indicators of stability to aerobic exposure. One of the empirical problems faced by the producer is to solve the question of how to avoid deterioration of the silage once it is opened, for which this research points to the resistance in the period of aerobic exposure (PEA) of a silage of Pennisetum purpureum (PP) with different levels of substitution of Tithonia diversifolia (TD). The study was conducted at the International Center for Tropical Agriculture in Colombia. With a 90-day fermentation silage, a seven-day aerobic stability test was carried out, using a kilogram silage at different proportions (%) of TD/PP: 100/0, 67/33, 33/67 and 0/100 enriched with two Lactobacillus-based additives. The change in dry matter, temperature and pH was measured, finding stability to aerobic exposure. The role of TD is remarkable once the silage enters the PEA due to the buffer capacity that buffers the change in pH once the silo is opened, generating greater aerobic stability.