Tuna trimmings (Thunnas albacares) hydrolysate alleviates immune stress and intestinal mucosal injury during chemotherapy on mice and identification of potentially active peptides.

In this study, Tuna trimmings (Thunnas albacares) protein hydrolysate (TPA) was produced by alcalase. The anti-tumor synergistic effect and intestinal mucosa protective effect of TPA on S180 tumor-bearing mice treated with 5-fluorouracil (5-FU) chemotherapy were investigated. The results showed that TPA can enhance the anti-tumor effect of 5-FU chemotherapy, as evident by a significant reduction in tumor volume observed in the medium and high dose TPA+5-FU groups compared to the 5-FU group (p < 0.001). Moreover, TPA significantly elevated the content of total protein and albumin in all TPA dose groups (p < 0.01, p < 0.001), indicating its ability to regulate the nutritional status of the mice. Furthermore, histopathological studies revealed a significant increase in the height of small intestinal villi, crypt depth, mucosal thickness, and villi area in the TPA+5-FU groups compared to the 5-FU group (p < 0.05), suggesting that TPA has a protective effect on the intestinal mucosa. Amino acid analysis revealed that TPA had a total amino acid content of 66.30 g/100 g, with essential amino acids accounting for 30.36 g/100 g. Peptide molecular weight distribution analysis of TPA indicated that peptides ranging from 0.25 to 1 kDa constituted 64.54%. LC-MS/MS analysis identified 109 peptide sequences, which were predicted to possess anti-cancer and anti-inflammatory activities through database prediction. Therefore, TPA has the potential to enhance the antitumor effects of 5-FU, mitigate immune depression and intestinal mucosal damage induced by 5-FU. Thus, TPA could be serve as an adjuvant nutritional support for malnourished patients undergoing chemotherapy.

Comparison and Evaluation of GHG Emissions during Simulated Thermophilic Composting of Different Municipal and Agricultural Feedstocks.

Composting is widely used to recycle a variety of different organic wastes. In this study, dairy manure, chicken litter, biosolids, yard trimmings and food waste were selected as representative municipal and agricultural feedstocks and composted in simulated thermophilic composting reactors to compare and evaluate the GHG emissions. The results showed that the highest cumulative emissions of CO2, CH4 and N2O were observed during yard trimmings composting (659.14 g CO2 kg-1 DM), food waste composting (3308.85 mg CH4 kg-1 DM) and chicken litter composting (1203.92 mg N2O kg-1 DM), respectively. The majority of the carbon was lost in the form of CO2. The highest carbon loss by CO2 and CH4 emissions and the highest nitrogen loss by N2O emission occurred in dairy manure (41.41%), food waste (0.55%) and chicken litter composting (3.13%), respectively. The total GHG emission equivalent was highest during food waste composting (365.28 kg CO2-eq ton-1 DM) which generated the highest CH4 emission and second highest N2O emissions, followed by chicken litter composting (341.27 kg CO2-eq ton-1 DM), which had the highest N2O emissions. The results indicated that accounting for GHG emissions from composting processes when it is being considered as a sustainable waste management practice was of great importance.

Investigation on pyrolysis and incineration of chrome-tanned solid waste from tanneries for effective treatment and disposal: an experimental study.

Chrome-tanned leather solid wastes (leather finished trimmings (LFT) and chrome shavings (CS)) from tanneries were studied using pyrolysis and incineration. Detailed characterization of CS and LFT indicated higher calorific value of 15.77 MJ/kg and 19.97 MJ/kg respectively, which makes it suitable for thermal treatment. Thermal Gravimetric Analysis (TGA) of CS and LFT recorded a weight loss of 79.82% and 68.22% at 800 °C respectively. Energy-dispersive X-ray spectroscopy and scanning electron microscopy analysis for CS and LFT were also carried out. Pyrolysis of CS and LFT was carried out using a fixed bed-type pyrolysis unit at a temperature of 500 ± 10 °C for a reaction time of 30 min and three different by-products (bio-oil, biochar and pyrolytic gas) were obtained as a result of pyrolysis. From pyrolysis process, higher bio-oil yields of 52 wt.% and 49 wt.% from LFT and CS with calorific value of 28.0 and 27.8 MJ/kg respectively were obtained. The calorific values of the biochar obtained from LFT and CS were found to be 20.5 and 23.0 MJ/kg respectively. Incineration was carried out in the existing incineration facility of 150 kg/h capacity at a temperature of 1200 °C. The results of incineration process showed a higher weight reduction (93.0 wt.%) and higher concentration of gaseous emissions, revealing the need for off-gas treatment. Further, FT-IR spectra of residual ash from the incineration process revealed the occurrence of oxidation of trivalent chromium to its hexavalent form, which could be a potential raw material in the metallurgical/chemical industry for the synthesis of sodium chromate or ferrochrome alloy. Comparative experimental investigations of pyrolysis and incineration revealed that incineration could be a potential treatment and disposal option, in developing countries like India, for chrome-tanned leather solid wastes from tanneries, for producing heat energy and the residue with potential utilization viability in another industry paving a way towards circular economy.

High-pressure processing of usually discarded dry aged beef trimmings for subsequent processing.

In this study, we investigated if the usually discarded trimmings from dry aged beef can be incorporated into raw fermented sausages as a substitute for fresh beef without altering any major characteristics. Dry aged trimmings were subjected to high-pressure processing (600 MPa, 3 min hold) to reduce the bacterial load, achieving a 3-log reduction. HPP-treated dry aged beef trimmings were then incorporated into raw fermented sausages (60% pork and 40% beef). Beef was substituted with trimmings in different concentrations (7.5, 12.5, 25, 50, 100%). Due to the substitution, the water content of the sausages was reduced depending on the amount of beef substituted. Consequently, the sausages with substituted beef, for example, 50 and 100%, achieved the same water content after 5.4 and 3.7 days, respectively, than control sausage at day 9. However, the substitution (100%) affected the fat content, which contributes to significant differences (p < .05) in firmness during ripening.

Leather solid waste: An eco-benign raw material for leather chemical preparation - A circular economy example.

One of the long lasting problems associated with leather industry is to meet environmental standards for both liquid and solid wastes. Statistics show that one tonne of wet-salted hides/skins yields around 650 kg of solid waste. Among various wastes generated, trimmings for the most part have been underutilized. Collagen presents in trimmings waste are effectively used but hair goes unutilized or at the most as feed for boilers during gelatin manufacturing. Hence, newer technology is needed for complete and effective utilization of raw trimmings. In leather manufacture, formaldehyde condensates polymers are used as re-tanning agent to enhance the compaction of leather. However, these products are hard for biodegradation and also cause the release of free formaldehyde in leather, which is a known carcinogen. Here, there is a need for development of formaldehyde free re-tanning agent for eco-benign leather processing. In this work an attempt had been made to develop formaldehyde free biodegradable eco-benign re-tanning agent from raw trimming of tannery solid waste as a circular economy model. Alkaline (7.5%w/w NaOH) - hydrogen peroxide (10%w/w) pre-treatment followed by thermal hydrolysis at 100 °C for 5 h was an optimized method for effective hydrolysis of trimmings and the process of preparation of product results in the holistic utilization of raw trimmings. The developed product was characterized using Dynamic light scattering and FTIR techniques. The product prepared was further used in leather manufacture as a re-tanning agent and was found to impart multifunctional properties to leathers such as fullness, grain tightness and shade of dye brilliance. Product improves the mechanical strength characteristics of leather and also the exhaustion of post-tanning chemicals. SEM analysis shows that the experimental leather is more compact and flat than control. This novel strategy had not only solved the issue of solid waste but also resulted in a greener leather auxiliary leading to greener environment.

Techno-economic analyses of solid-state anaerobic digestion and composting of yard trimmings.

Solid-state anaerobic digestion (SS-AD) and composting are two potential alternatives to divert yard trimmings from landfills. This study aimed to evaluate the techno-economic feasibility of commercial-scale SS-AD and composting systems (20,000 metric tons (MT)/year) that received both yard trimmings and liquid AD effluent using a modeling software, SuperPro Designer. Both the SS-AD and composting systems were shown to be economically feasible. While their revenues were comparable ($48/MT), SS-AD with digestate drying showed a higher capital cost ($256/MT vs. $84/MT) but a lower non-facility-dependent operating cost ($11/MT vs. $21/MT) than composting. The payback time, internal rate of return (IRR), and net present value (NPV) were estimated to be ∼10 years, 8%, and $0.2 million, respectively, for SS-AD, and ∼4.9 years, 33%, and $1.8 million, respectively, for composting. Digestate drying was necessary to make SS-AD profitable via the sale of byproduct, but it was also the most energy intensive step, relying on heat recovery to reduce costs. Moreover, the economics of SS-AD were highly improved (NPV $2 million) with financial incentives (i.e. investment tax credits), indicating that incentives were critical to the economic feasibility of current SS-AD systems that utilize lignocellulosic biomass. However, renewable identification numbers (RINs) and renewable energy certificates (RECs) had minor effects. Furthermore, the economics of both systems were most sensitive to plant size, tipping fees, and byproduct/compost price. The results suggest SS-AD may be favored for centralized management while composting for de-centralized management of yard trimmings. Alternative ways to valorize digestate should be evaluated in future studies.

Screening of a microbial consortium for selective degradation of lignin from tree trimmings.

To acquire microbial consortia with effectively precedent degradation of lignin, samples obtained from rotten trunks, rotten stumps and soil near it were screened and isolated after generations of subculture. The dynamic change illustrated that their community structures were affected by pH and tended to be stable after 6 days' cultivation. The desired one, named DM-1, was gained through successive cultivation for over 5 generations, whose high selectivity in lignin degradation was observed within 16 days' cultivation (SV = 2.78). Meanwhile, a remarkable reduction in the fiber crystallinity of tree trimmings (10.35%) resulted from the bio-degradation of DM-1, displayed a greater exposure of cellulose by selective removal of lignin. The diversity analysis of DM-1 was investigated by PCR amplification and 16S rDNA sequencing, indicated that mesorhizobium, cellulosimicrobium, pandoraea, achromobacter and stenotrophomones were the predominant genera. Furthermore, fungi (3 strains), bacteria (4 strains) and actinomycetes (5 strains) constituted 12 strains in total were gained by plate isolation from DM-1.

Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides.

Large quantities of low-value protein rich co-products, such as salmon skin and trimmings, are generated annually. These co-products can be upgraded to high-value functional ingredients. The aim of this study was to assess the antidiabetic potential of salmon skin gelatin and trimmings-derived protein hydrolysates in vitro. The gelatin hydrolysate generated with Alcalase 2.4L and Flavourzyme 500L exhibited significantly higher (p < 0.001) insulin and GLP-1 secretory activity from pancreatic BRIN-BD11 and enteroendocrine GLUTag cells, respectively, when tested at 2.5 mg/mL compared to hydrolysates generated with Alcalase 2.4L or Promod 144MG. The gelatin hydrolysate generated with Alcalase 2.4L and Flavourzyme 500L showed significantly more potent (p < 0.01) DPP-IV inhibitory activity than those generated with Alcalase 2.4L or Promod 144MG. No significant difference was observed in the insulinotropic activity mediated by any of the trimmings-derived hydrolysates when tested at 2.5 mg/mL. However, the trimmings hydrolysate generated with Alcalase 2.4L and Flavourzyme 500L exhibited significantly higher DPP-IV inhibitory (p < 0.05:Alcalase 2.4L and p < 0.01:Promod 144MG) and GLP-1 (p < 0.001, 2.5 mg/mL) secretory activity than those generated with Alcalase 2.4L or Promod 144MG. The salmon trimmings hydrolysate generated with Alcalase 2.4L and Flavourzyme 500L when subjected to simulated gastrointestinal digestion (SGID) was shown to retain its GLP-1 secretory and DPP-IV inhibitory activities, in addition to improving its insulin secretory activity. However, the gelatin hydrolysate generated with Alcalase 2.4L and Flavourzyme 500L was shown to lose GLP-1 secretory activity following SGID. A significant increase in membrane potential (p < 0.001) and intracellular calcium (p < 0.001) by both co-product hydrolysates generated with Alcalase 2.4L and Flavourzyme 500L suggest that both hydrolysates mediate their insulinotropic activity through the KATP channel-dependent pathway. Additionally, by stimulating a significant increase in intracellular cAMP release (p < 0.05) it is likely that the trimmings-derived hydrolysate may also mediate insulin secretion through the protein kinase A pathway. The results presented herein demonstrate that salmon co-product hydrolysates exhibit promising in vitro antidiabetic activity.

Novel Continuous and Manual Sampling Methods for Beef Trim Microbiological Testing.

A sampling method that represents a greater proportion of the beef trimmings in a 900-kg combo bin should improve the current pathogen sampling and detection programs used by fresh beef processors. This study compared two novel, nondestructive sampling methodologies (a continuous sampling device [CSD] and a manual sampling device [MSD]) with the current industry methodologies, the N60 Excision (the "gold standard") and N60 Plus, for collection of trim samples. Depending on the experiment, samples were analyzed for naturally occurring Escherichia coli O157:H7 or Salmonella, inoculated surrogates, or indicator organisms in multiple plants, on multiple days, across multiple lean percentage mixtures. Experiments 1A and 1B with natural contamination found no E. coli O157:H7 but similar ( P > 0.05) prevalence of Salmonella (CSD 9.2% versus N60 Excision 6.0%) and similar ( P > 0.05) levels of indicator organisms for CSD compared with both N60 methodologies. In experiments 2 and 3, CSD cloth sampling had the same or higher prevalence of naturally occurring E. coli O157:H7 and E. coli O157:H7 surrogate organisms, as well as similar levels of indicator organisms compared with the N60 methodologies. In experiment 4, MSD cloth sampling detected similar ( P > 0.05) prevalence of E. coli O157:H7 surrogate organisms, as well as slightly lower ( P < 0.05) levels of indicator organisms compared with N60 Plus. In experiment 5, the MSD found similar ( P > 0.05) prevalence of naturally occurring E. coli O157:H7 and the same or slightly higher ( P < 0.05) levels of naturally occurring indicator organisms compared with N60 Plus. In experiment 6, the MSD detected the same ( P > 0.05) prevalence of naturally occurring Salmonella as did N60 Excision. The results of these experiments collectively demonstrate that sampling beef trim using either the CSD or MSD provides organism recovery that is similar to or better than the N60 Excision or the N60 Plus methodologies.

Bioactive peptides from Atlantic salmon (Salmo salar) with angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory, and antioxidant activities.

The pH shift method was utilised for the recovery of proteins from salmon trimmings (ST), yielding 93% (w/w) protein. ST protein (STP) hydrolysates were generated with different enzyme preparations. STP incubated with Corolase PP for 1h (STP-C1) had the most potent angiotensin converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) inhibitory and oxygen radical absorbance capacity (ORAC) activities. Analysis of fractions of STP-C1 using UPLC-MS/MS identified sixteen peptides/amino acids. Tyr-Pro had the highest ACE inhibitory activity (ACE IC50=5.21±0.94µM). The highest DPP-IV inhibitory activity was found with the amino acid Tyr (DPP-IV IC50=75.15±0.84µM). Val-Pro had the highest ORAC activity (19.45±2.15µmol of TEg-1). To our knowledge, the peptides Gly-Pro-Ala-Val, Val-Cys, and Phe-Phe have not been previously identified to have the activities tested in this study. These results indicate that STP hydrolysates are potential sources of bioactive peptides.

New insights into meat by-product utilization.

Meat industry generates large volumes of by-products like blood, bones, meat trimmings, skin, fatty tissues, horns, hoofs, feet, skull and viscera among others that are costly to be treated and disposed ecologically. These costs can be balanced through innovation to generate added value products that increase its profitability. Rendering results in feed ingredients for livestock, poultry and aquaculture as well as for pet foods. Energy valorization can be obtained through the thermochemical processing of meat and bone meal or the use of waste animal fats for the production of biodiesel. More recently, new applications have been reported like the production of polyhydroxyalkanoates as alternative to plastics produced from petroleum. Other interesting valorization strategies are based on the hydrolysis of by-products to obtain added value products like bioactive peptides with relevant physiological effects as antihypertensive, antioxidant, antidiabetic, antimicrobial, etc. with promising applications in the food, pharmaceutical and cosmetics industry. This paper reports and discusses the latest developments and trends in the use and valorisation of meat industry by-products.

Fungal pretreatment of unsterilized yard trimmings for enhanced methane production by solid-state anaerobic digestion.

Fungal pretreatment is an environmentally friendly process that has been widely studied to improve the digestibility of lignocellulosic biomass. However, sterilization of feedstocks, a costly process, is generally required prior to the fungal pretreatment. In this study, fungal pretreatment of unsterilized yard trimmings using yard trimmings pre-colonized with Ceriporiopsis subvermispora as an inoculum was investigated. Degradation of lignin, cellulose, hemicellulose, and dry matter in yard trimmings during 30 days of fungal pretreatment using different inoculum/substrate ratios (1:19, 1:9 and 1:4) was 14.8-20.2%, 8.1-15.4%, 20.7-27.8%, and 9.8-16.2%, respectively. Methane yields of 34.9-44.6L/kg volatile solids were achieved during solid-state anaerobic digestion (SS-AD) of the pretreated yard trimmings, which were comparable to those obtained by using the traditional method requiring feedstock sterilization. The technology developed in this study can save about 501-789 kJ/kg of dry yard trimmings processed, which is about half of the total biogas energy produced by SS-AD.

Use of mild irradiation doses to control pathogenic bacteria on meat trimmings for production of patties aiming at provoking minimal changes in quality attributes.

The objectives of the present work were to assess the use of moderate doses of gamma irradiation (2 to 5 kGy) and to reduce the risk of pathogen presence without altering the quality attributes of bovine trimmings and of patties made of irradiated trimmings. Microbiological indicators (coliforms, Pseudomonas spp and mesophilic aerobic counts), physicochemical indicators (pH, color and tiobarbituric acid) and sensory changes were evaluated during storage. 5 kGy irradiation doses slightly increased off flavors in patties. Two pathogenic markers (Listeria monocytogenes and Escherichia coli O157:H7) were inoculated at high or low loads to trimming samples which were subsequently irradiated and lethality curves were obtained. Provided that using irradiation doses ≤2.5 kGy are used, reductions of 2 log CFU/g of L. monocytogenes and 5 log CFU/g of E. coli O157:H7 are expected. It seems reasonable to suppose that irradiation can be successfully employed to improve the safety of frozen trimmings when initial pathogenic bacteria burdens are not extremely high.

Solid-state anaerobic co-digestion of spent mushroom substrate with yard trimmings and wheat straw for biogas production.

Spent mushroom substrate (SMS) is a biomass waste generated from mushroom production. About 5 kg of SMS is generated for every kg of mushroom produced. In this study, solid state anaerobic digestion (SS-AD) of SMS, wheat straw, yard trimmings, and their mixtures was investigated at different feedstock to effluent ratios. SMS was found to be highly degradable, which resulted in inhibition of SS-AD due to volatile fatty acid (VFA) accumulation and a decrease in pH. This issue was addressed by co-digestion of SMS with either yard trimmings or wheat straw. SS-AD of SMS/yard trimmings achieved a cumulative methane yield of 194 L/kg VS, which was 16 and 2 times higher than that from SMS and yard trimmings, respectively. SS-AD of SMS/wheat straw obtained a cumulative methane yield of 269 L/kg VS, which was 23 times as high as that from SMS and comparable to that from wheat straw.

CARACTERIZACIÓN DE COPRODUCTOS DE LA INDUSTRIA DEL FILETEADO DE TILAPIA NILÓTICA (Oreochromis niloticus) Y TRUCHA ARCOÍRIS (Oncorhynchus mykiss) EN COLOMBIA / CHARACTERIZATION OF BYPRODUCTS FROM THE FILLETING INDUSTRY OF NILE TILAPIA (Oreochromis niloticus) AND RAINBOW TROUT (Oncorhynchus mykiss) IN COLOMBIA

La industria del fileteado de tilapia y trucha genera cantidades significativas de coproductos de los cuales no existe suficiente información disponible sobre su composición y calidad. Esta investigación determinó algunos componentes nutricionales y microbiológicos de coproductos obtenidos en plantas exportadoras de filetes de tilapia nilótica y trucha arcoíris en Colombia. El esqueleto mostró el mayor aporte de cenizas (tilapia: 14,12 ± 0,16%; trucha: 6,18 ± 0,06%), calcio (tilapia: 5,70 ± 0,04%; trucha: 1,72 ± 0,08%) y fósforo (tilapia: 1,89 ± 0,06%; trucha: 0,84 ± 0,03%), mientras que los recortes presentaron la mayor concentración de extracto etéreo (tilapia: 30,10 ± 0,58%; trucha: 17,50 ± 0,51%) y la carne mecánicamente separada, los mayores contenidos de humedad (tilapia: 70,38 ± 0,18%; trucha: 72,93 ± 0,3%) y proteína (tilapia: 14,32 ± 0,03%; trucha: 18,88 ± 0,05%). El mayor aporte de ácidos grasos polinsaturados (AGPI), como porcentaje del total de ácidos grasos identificados, se encontró en esqueleto de tilapia (17,27%) y en recortes de trucha (38,68%), con relaciones n6/n3 de 2,67 y 0,71, respectivamente. En general, los coproductos estudiados cumplieron con los estándares microbiológicos exigidos por la normatividad colombiana (Resolución 122 de 2012 del Ministerio de Salud y Protección Social) que establece los requisitos fisicoquímicos y microbiológicos que deben cumplir los productos de la pesca, en particular pescados, moluscos y crustáceos para consumo humano. Los resultados muestran que los coproductos estudiados contienen un importante aporte de proteína, cenizas, ácidos grasos poli-insaturados y adecuada calidad microbiológica, con potencial para alimentación humana o animal.

The filleting industry of tilapia and rainbow trout generates a great amount of byproducts; there is not information available about their composition and quality. The objective of this research was to determine the byproducts nutritional and microbiological components obtained from the fillets export industry of Nile tilapia and rainbow trout in Colombia. The skeleton showed a high amount of ashes (tilapia: 14.12 ± 0.16%; trout: 6.18 ± 0.06%), calcium (tilapia: 5.70 ± 0.04%; trout: 1.72 ± 0.08%) and phosphorus (tilapia: 1.89 ± 0.06%; trout: 0.84 ± 0.03%), mean while the trimmings had the highest concentration of ether extract (tilapia: 30.10 ± 0.58%; trout: 17.50 ± 0.51%) and the mechanically deboned meat, the higher moisture content (tilapia: 70.38 ± 0.18%; trout: 72.93 ± 0.3%) and protein (tilapia: 14.32 ± 0.03%; trout: 18.88 ± 0.05%). The highest contribution of polyunsaturated fatty acids (PUFA) as a percentage of total fatty acids, was found in the skeleton of the tilapia (17.27%) and trout trimmings (38.68%), with a ratio n6/n3 of 2.67 y 0.71 respectively. In general, the byproducts analyzed met the microbiological standards required by Colombian government, in resolution 122/2012 - Ministry of Social Welfare and Health, by which technical regulations on the phyponents obtained from the fillets export industry of Nile tilapia and rainbow trout in sicochemical and microbiological requirements for fishery products, particularly fish, mollusks and crustaceans for human consumption. The results show that byproducts studied could be an important source of protein, ash, polyunsaturated fatty acids with and appropriate microbiological quality, showing potential for human food or animal feed.