Ganglioside GD3 Regulates Inflammation and Epithelial-to-Mesenchymal Transition in Human Nasal Epithelial Cells.

Chronic sinusitis with nasal polyps (CRSwNP) is one of the most common chronic inflammatory diseases, and involves tissue remodeling. One of the key mechanisms of tissue remodeling is the epithelial-mesenchymal transition (EMT), which also represents one of the pathophysiological processes of CRS observed in CRSwNP tissues. To date, many transcription factors and forms of extracellular stimulation have been found to regulate the EMT process. However, it is not known whether gangliosides, which are the central molecules of plasma membranes, involved in regulating signal transmission pathways, are involved in the EMT process. Therefore, we aimed to determine the role of gangliosides in the EMT process. First, we confirmed that N-cadherin, which is a known mesenchymal marker, and ganglioside GD3 were specifically expressed in CRSwNP_NP tissues. Subsequently, we investigated whether the administration of TNF-α to human nasal epithelial cells (hNECs) resulted in the upregulation of ganglioside GD3 and its synthesizing enzyme, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialytransferase 1 (ST8Sia1), and the consequently promoted inflammatory processes. Additionally, the expression of N-cadherin, Zinc finger protein SNAI2 (SLUG), and matrix metallopeptidase 9 (MMP-9) were elevated, but that of E-cadherin, which is known to be epithelial, was reduced. Moreover, the inhibition of ganglioside GD3 expression by the siRNA or exogenous treatment of neuraminidase 3 (NEU 3) led to the suppression of inflammation and EMT. These results suggest that gangliosides may play an important role in prevention and therapy for inflammation and EMT.

Effect of glucocorticoids infiltration on CRSwNP after endoscopic sinus surgery and the curative efficacy of nasal ventilation function and mucociliary clearance.

OBJECTIVES: This study investigated and analyzed the effects of glucocorticoid infiltration on chronic rhino-sinusitis with nasal polyps (CRSwNP) after endoscopic sinus surgery (ESS) and its curative efficacy on nasal ventilation function and mucociliary clearance (MCC). METHODS: 126 CRSwNP patients admitted to the hospital from March 2018 to May 2020 were enrolled and randomly divided into observation group (n=65) and control group (n=61) based on random number table. The control group received ESS, and the observation group was given glucocorticoids treatment after ESS. The changes of nasal ventilation function, MCC and quality of life between the two groups of patients before and after treatment were compared. RESULTS: The overall effective rate of clinical therapy was critically higher in observation group than in control group (P<0.05). In addition, NMCA and NCV in observation group were critically higher than those in control group (P<0.05), and nasal airway resistance (NAR) in observation group was notably lower than that in control group (P<0.05). In addition, the Saccharin removal time in observation group after treatment was remarkably lower than that in control group (P<0.05), while the speed and rate of MCC were critically higher than those in control group (P<0.05). Finally, the scores of each dimension of WHOQOL-100 scale in two groups of subjects after treatment were critically higher than those before treatment (P<0.05), and the scores in observation group were notably higher than those in control group (P<0.05). CONCLUSION: The treatment of glucocorticoid infiltration on CRSwNP after ESS can effectively improve the curative effect. It improves the patient's function of nasal ventilation and MCC, thus beneficial to promoting the sufferers' living quality and is worthy of clinical promotion.