The ring-legged earwig Euborellia annulipes as a new model for oogenesis and development studies in insects.

Insects are the dominant group of animals on Earth. Despite this abundance, most of our knowledge about many aspects of their biology and development come from a unique model, the vinegar fly, Drosophila melanogaster. Nevertheless, in the last years, the advances in molecular tools and imaging techniques have allowed the emergence of new insect models, adding valuable information to decipher the morphogenetic bases behind the formation and evolution of the vast diversity of shapes, sizes, and patterns that characterize them. Earwigs belong to Dermaptera which is a small order clustered in the Polyneopteran group. They are hemimetabolous insects with a flattened body, characteristic abdominal pincers, and maternal care behavior. This last feature and their role in agroecosystems have been studied in cosmopolitan species such as Forficula auricularia and Euborellia annulipes; however, their reproduction and embryonic development have been poorly addressed in laboratory conditions. In response, here we describe the ring-legged earwig Euborellia annulipes embryogenesis and life cycle from nymphal to adult stages, its reproduction, and essential morphological and behavioral characters. Additionally, using confocal and transmission electron microscopy we analyzed in detail the morphogenesis of its peculiar meroistic polytrophic ovary. Our aim is to provide an emerging model system to perform comparative studies on insect oogenesis, development, and morphological evolution.

Cell behaviors that pattern developing tissues: the case of the vertebrate nervous system.

Morphogenesis from cells to tissue gives rise to the complex architectures that make our organs. How cells and their dynamic behavior are translated into functional spatial patterns is only starting to be understood. Recent advances in quantitative imaging revealed that, although highly heterogeneous, cellular behaviors make reproducible tissue patterns. Emerging evidence suggests that mechanisms of cellular coordination, intrinsic variability and plasticity are critical for robust pattern formation. While pattern development shows a high level of fidelity, tissue organization has undergone drastic changes throughout the course of evolution. In addition, alterations in cell behavior, if unregulated, can cause developmental malformations that disrupt function. Therefore, comparative studies of different species and of disease models offer a powerful approach for understanding how novel spatial configurations arise from variations in cell behavior and the fundamentals of successful pattern formation. In this chapter, I dive into the development of the vertebrate nervous system to explore efforts to dissect pattern formation beyond molecules, the emerging core principles and open questions.

Comparative Studies of Gene Expression Kinetics: Methodologies and Insights on Development and Evolution.

Across the animal kingdom, embryos of closely related species show high morphological similarity despite genetic and environmental distances. Deciphering the molecular mechanisms that underlie morphological conservation and those that support embryonic adaptation are keys to understand developmental robustness and evolution. Comparative studies of developmental gene regulatory networks can track the genetic changes that lead to evolutionary novelties. However, these studies are limited to a relatively small set of genes and demand extensive experimental efforts. An alternative approach enabled by next-generation sequencing, is to compare the expression kinetic of large sets of genes between different species. The advantages of these comparisons are that they can be done relatively easily, for any species and they provide information of all expressed genes. The challenge in these experiments is to compare the kinetic profiles of thousands of genes between species that develop in different rates. Here we review recent comparative studies that tackled the challenges of accurate staging and large-scale analyses using different computational approaches. These studies reveal how correct temporal scaling exposes the striking conservation of developmental gene expression between morphologically similar species. Different clustering approaches are used to address various comparative questions and identify the conservation and divergence of large gene sets. We discuss the unexpected contribution of housekeeping genes to the interspecies correlations and how this contribution distorts the hourglass pattern generated by developmental genes. Overall, we demonstrate how comparative studies of gene expression kinetics can provide novel insights into the developmental constraints and plasticity that shape animal body plans.

Corrigendum: Comparative Studies of Gene Expression Kinetics: Methodologies and Insights on Development and Evolution.

[This corrects the article DOI: 10.3389/fgene.2018.00339.].

Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution.

Developmental biology, as all experimental science, is empowered by technological advances. The availability of genetic tools in some species - designated as model organisms - has driven their use as major platforms for understanding development, physiology and behavior. Extending these tools to a wider range of species determines whether (and how) we can experimentally approach developmental diversity and evolution. During the last two decades, comparative developmental biology (evo-devo) was marked by the introduction of gene knockdown and deep sequencing technologies that are applicable to a wide range of species. These approaches allowed us to test the developmental role of specific genes in diverse species, to study biological processes that are not accessible in established models and, in some cases, to conduct genome-wide screens that overcome the limitations of the candidate gene approach. The recent discovery of CRISPR/Cas as a means of precise alterations into the genome promises to revolutionize developmental genetics. In this review we describe the development of gene editing tools, from zinc-finger nucleases to TALENs and CRISPR, and examine their application in gene targeting, their limitations and the opportunities they present for evo-devo. We outline their use in gene knock-out and knock-in approaches, and in manipulating gene functions by directing molecular effectors to specific sites in the genome. The ease-of-use and efficiency of CRISPR in diverse species provide an opportunity to close the technology gap that exists between established model organisms and emerging genetically-tractable species.