Effect of the pelleting process on diet formulations with varying levels of crystalline amino acids and reducing sugars on digestibility in growing pigs.

The objective of this experiment was to determine the effects of pelleting on the standardized ileal digestibility (SID) of amino acids (AA) and crude protein (CP) in diets with or without increased concentrations of free AA and reducing sugars (RS). Eight individually housed, ileal cannulated barrows (initially 31.4 kg) were allotted to an 8 × 8 Latin square with eight diets and eight 7-d periods with ileal digesta collected on days 6 and 7. Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of diet form (mash or pellet), crystalline AA (low or high), or RS (low or high), provided by distillers dried grains with solubles and bakery meal. Diets were pelleted to achieve a hot pellet temperature of 85 to 88 °C. Data were analyzed as a Latin square design using the GLIMMIX procedure of SAS 9.4. A feed form × RS interaction (P < 0.026) for SID of tryptophan was observed. Feeding pelleted low RS diets increased SID of tryptophan compared with mash high and low RS diets, and pelleted high RS diets. For the main effects of feed form, the SID of total AA, CP, and indispensable AA was greater (P < 0.042) in pelleted diets compared with mash diets. For the main effects of crystalline AA, pigs fed high crystalline AA had increased (P = 0.007) SID of tryptophan and decreased (P = 0.050) SID of histidine compared with those fed low crystalline AA diets. For the main effects of RS, high RS diets had decreased (P < 0.05) SID of total AA, CP, and indispensable AA compared with low RS diets. In conclusion, pelleting diets increased AA digestibility, and pelleting diets with increased crystalline AA or RS did not affect the improvement in AA digestibility from pelleting. Diets formulated with high crystalline AA had increased SID of tryptophan. Formulating diets with high RS resulted in decreased AA digestibility compared with corn-soybean meal-based diets.

The objective of this study was to determine the effects of pelleting on the standardized ileal digestibility (SID) of amino acids (AA) in diets with or without increased concentrations of free AA and reducing sugars (RS). Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of diet form (mash or pellet), crystalline AA (low or high), or RS (low or high), provided by dried distillers grains with solubles and bakery meal. A total of 8 illeal cannulated barrows were fed treatments in an 8 × 8 Latin square design. Results indicated that there was no evidence of interactions between diet types and diet form, indicating that increasing amounts of crystalline AA and RS did not reduce amino acid digestibility when pelleting diets. Additionally, pelleting diets resulted in increased amino acid digestibility compared to mash diets. Diets formulated with 20% dried distillers grains with solubles and 15% bakery resulted in decreased amino acid digestibility compared with the corn­soybean meal-based diets. Crystalline amino acid concentration did not influence amino acid digestibility of indispensable AA, except for SID of tryptophan which was increased in diets with higher concentrations of crystalline AA.

An Overview of Parenteral Nutrition from Birth to Adolescence Based on a Composite Fish Oil Containing Lipid Emulsion and a Pediatric Amino Acid Solution.

Intestinal failure (IF) is characterized by a critical reduction in functional gut mass below the minimum needed for optimal growth in children. It requires parenteral nutrition (PN) and home-PN (HPN), which is challenging in terms of meeting nutritional needs according to age, growth velocity, clinical situation, and rapid changes in fluid and electrolyte requirements. Due to these complex requirements, age-adapted multi-chamber bags (MCBs) are important additions to the nutrition armamentarium. The launch of composite fish oil (FO)-containing intravenous lipid emulsions (ILEs) heralded the development of MCBs containing these ILEs in combination with a crystalline amino acid solution adapted for pediatric use. The safety and efficacy of lipid and amino acid components in this context have been widely documented in numerous published studies. This narrative manuscript includes a review of the articles published in PudMed, Embase, and Google Scholar up to June 2023 for the age groups of term infants to children and adolescents. Preterm infants with their highly specific demands are not included. It aims to offer an overview of the clinical experience regarding the use of a composite FO-based ILE and a developed specific amino acid solution.

Effects of dietary protein content and crystalline amino acid supplementation patterns on growth performance, intestinal histomorphology, and immune response in weaned pigs raised under different sanitary conditions.

The aim of this experiment was to investigate the effects of dietary crude protein (CP) contents and crystalline amino acids (CAA) supplementation patterns on growth performance, intestinal histomorphology, and immune response in weaned pigs under clean (CSC) or unclean sanitary conditions (USC). A total of 144 weaned pigs (6.35 ± 0.63 kg body weight) were assigned to 6 treatments in a 3 × 2 factorial arrangement based on CP content and sanitary conditions using a randomized complete block design, giving 8 replicates per treatment with 3 pigs per pen. Pigs were fed one of three diets for 21 d: one high CP (HCP; 22%) and two low CP (LCP; 19%) diets supplemented with 9 indispensable AA or only 6 AA (Lys, Met, Thr, Trp, Val, and Ile) as CAA. The CSC room was washed weekly, whereas the USC room had sow manure spread in the pens and was not washed throughout the experiment. Body weight and feed disappearance were recorded weekly. Blood was sampled from 1 pig per pen weekly, and the same pig was euthanized for jejunal tissues sampling on day 21. Pigs raised under USC had reduced (P < 0.05) average daily gain (ADG) and gain to feed ratio (G:F) in week 2, but contrary results that greater (P < 0.05) ADG and G:F were found in pigs under USC in week 3. Overall, there was an interaction where G:F did not differ between HCP and LCP under CSC, however, LCP decreased (P < 0.05) G:F compared to HCP under USC. Pigs fed the HCP diet had higher (P < 0.05) fecal scores than those fed the LCP diets throughout the experiment. Pigs fed the LCP had higher (P < 0.05) villus height to crypt depth ratio than those fed the HCP. An interaction was observed where goblet cell density in the jejunum was higher (P < 0.05) in pigs fed LCP than HCP under CSC, but no difference was found between HCP and LCP under USC. Different CAA supplementation patterns did not influence both growth performance and histomorphology. Pigs raised under USC had greater (P < 0.05) plasma interleukin (IL)-10 and IL-6 concentrations and reduced (P < 0.05) plasma tumor necrosis factor-alpha concentration. Also, the LCP diets resulted in a greater (P < 0.05) plasma IL-10 concentration. In conclusion, overall growth performance did not differ between HCP and LCP under CSC, but LCP diets reduced G:F under USC. Feeding LCP diets to weaned pigs improved gut morphology under USC and ameliorated systemic inflammation induced by USC, whereas CAA supplementation patterns did not affect growth performance and gut morphology.

Reducing dietary crude protein (CP) content reduces nitrogen excretion to the environment and undigested protein availability to enteric pathogens in the hind gut resulting in reduced postweaning diarrhea and improved gut health. To meet the amino acid requirements in low CP diets, supplementation of essential amino acids in nursery diets is necessary, and the availability of more feed-grade crystalline amino acids allows low CP diets to be practically formulated. The sanitation challenge model mimics commercial raising conditions and stimulates a low-grade inflammatory and immune response. Although low CP diets decreased the feed efficiency in pigs raised under unclean conditions in this study, improved gut histomorphology and ameliorated inflammatory response were obtained in pigs fed low CP diets. However, no effect was found in crystalline amino acids supplementation patterns. The outcomes of this study confirmed that low CP diets could be used to improve gut health and ameliorate immune response in weaned pigs.

New perspectives for evaluating relative risks of African swine fever virus contamination in global feed ingredient supply chains.

There are no published reports indicating that the African swine fever virus (ASFV) has been detected in feed ingredients or complete feed. This is primarily because there are only a few laboratories in the world that have the biosecurity and analytical capabilities of detecting ASFV in feed. Several in vitro studies have been conducted to evaluate ASFV concentration, viability and inactivation when ASFV was added to various feed ingredients and complete feed. These inoculation studies have shown that some feed matrices support virus survival longer than others and the reasons for this are unknown. Current analytical methodologies have significant limitations in sensitivity, repeatability, ability to detect viable virus particles and association with infectivity. As a result, interpretation of findings using various measures may lead to misleading conclusions. Because of analytical and technical challenges, as well as the lack of ASFV contamination data in feed supply chains, quantitative risk assessments have not been conducted. A few qualitative risk assessments have been conducted, but they have not considered differences in potential scenarios for ASFV contamination between various types of feed ingredient supply chains. Therefore, the purpose of this review is to provide a more holistic understanding of the relative potential risks of ASFV contamination in various global feed ingredient supply chains and provide recommendations for addressing the challenges identified.

Crystalline amino acids do not influence calculated values for standardized ileal digestibility of amino acids in feed ingredients included in diets for pigs.

An experiment was conducted to test the hypothesis that addition of crystalline amino acids (AA) to diets during the adaptation or collection periods will not influence calculated values for apparent ileal digestibility (AID) or standardized ileal digestibility (SID) of AA in corn and soybean meal (SBM). Seven ileal-cannulated barrows (initial body weight: 77.9 ± 2.6 kg) were allotted to a 7 × 7 Latin Square design with 7 dietary treatments and 7 periods. Dietary treatments included feeding diets based on corn or SBM without or with crystalline AA for the entire 7-d period or with crystalline AA during the adaptation period, but without crystalline AA during the collection period. An N-free diet was also used. Each experimental period consisted of 5 d of adaptation to the diets followed by 2 d of ileal digesta collection, with digesta being collected for 9 hr/d starting after feeding the morning meal. Thus, the entire experiment lasted 49 d. The AID and SID of crude protein (CP) and AA were calculated using values determined in corn or in SBM without or with crystalline AA to determine if crystalline AA influenced calculated values for AID or SID of CP and AA. Results indicated that addition of crystalline AA to diets fed during the entire 7-d period increased (P < 0.05) AID of some AA in corn and SBM. However, no differences in SID of CP and AA were observed between pigs fed the diets without crystalline AA and pigs fed the diets with crystalline AA if dietary crystalline AA were ignored in calculations of SID values. This indicates that crystalline AA were 100% absorbed before the distal ileum and did not affect calculated values for SID of AA if calculations were based only on the AA in corn or SBM. Therefore, it is concluded that crystalline AA may be added to experimental diets in digestibility experiments before and during collection periods without affecting results, if crystalline AA are disregarded in the calculation of AID or SID of AA in ingredients.

Adición de glucomananos a dietas con baja proteína a base de sorgo-pasta de soya para cerdos en crecimiento y finalización / Addition of glucomannans to low-protein diets based on sorghum-soybean meal for growing and finishing pigs

Los cerdos en engorde alimentados con dietas sorgo-pasta de soya con baja proteína (DBP) presentan resultados productivos inconsistentes; en ocasiones, las variables productivas se mantienen, y en otras se deterioran. Además, siendo el sorgo el ingrediente en mayor cantidad, es probable que la dieta contenga mayor concentración de micotoxinas que en dietas estándar o en dietas formuladas con maíz. Por ello, se realizaron dos experimentos con cerdos en crecimiento y finalización, adicionando un secuestrante de micotoxinas (SECM) a dietas estándar y DBP para determinar su efecto en la respuesta productiva, características de la canal y urea en plasma. En el experimento de crecimiento se utilizaron 40 cerdos (machos castrados; 22,18 ± 0,82 kg) en un diseño de bloques completos al azar con arreglo factorial (2×4): dos niveles de proteína cruda (PC: 16; y 11,5%) y cuatro del SECM (0; 0,5; 1,0; 1,5 kg t-1), con cinco repeticiones por tratamiento. La reducción de PC disminuyó (P£0,05) la ganancia diaria de peso (GDP) y de carne magra (GCM), e incrementó (P£0,05) la conversión alimenticia (CA). En el experimento de finalización también se utilizaron 40 cerdos (machos castrados; 49,48 ± 1,10 kg). El diseño experimental fue similar al del experimento de crecimiento (14 y 9,5% PC; 0; 0,5; 1,0 y 1,5 kg t-1 SECM) y cinco repeticiones por tratamiento. La reducción de PC disminuyó (P£0,05) GDP, consumo de alimento (CAL) y GCM. En ambos experimentos, el SECM no mejoró las variables de respuesta en cerdos alimentados con DBP. Se concluye que la adición de SECM en DBP no mejora las variables productivas, pero mantiene las características de la canal, aunque reduce la GCM.

The growth performance of fattening pigs fed sorghum-soybean meal, low-protein diets (LPD) has been inconsistent; some times the productive variables do not change, but other times they are deteriorated. In addition, because the sorghum grain represents the main ingredient, it is likely that the diet has higher amounts of mycotoxins than standard diets or diets formulated with corn grain. So, two experiments were conducted with growing-finishing pigs to determine the effect of a mycotoxin sequestrant (MSEQ) added to standard or LPD on growth performance, carcass characteristics, and plasma urea nitrogen concentration. In the growing experiment, 40 growing (22.18 ± 0.82 kg) barrows were used in a randomized complete block design in a factorial (2×4) arrangement: two levels of crude protein (CP: 16 and 11.5%) and four levels of micotixins sequestrant (glucomannans; 0, 0.5, 1.0, 1.5 kg t-1), with five replicates per treatment. Lowering CP reduced (P£0.05) average daily gain (ADG) and fat free lean gain (FFLG), and increased (P£0,05) feed:gain ratio. In the finishing experiment, 40 finishing (49.48 ± 1.10 kg) barrows were used in a randomized complete block design in a factorial (2×4) arrangement: two levels of CP (14 and 9.5%) and four levels of micotoxins sequestrant (glucomannans; 0, 0.5, 1.0, 1.5 kg t-1), with five replicates per treatment. Diminishing CP reduced (P£0.05) ADG, average daily feed intake, and FFLG. The addition of glucomannans to low-protein diets did not affect growth performance in both experiments. It was concluded that adding glucomannans as mycotoxins sequestrant does not improve productive variables in pigs fed low-protein diets, but it produces similar carcass characteristics as in pigs fed standard diet, except the lower FFLG.

Respuesta productiva y concentración de urea en plasma de cerdos en crecimiento alimentados con dietas sorgo-pasta de soya con baja proteína / Growth performance and plasma urea concentration of growing pigs fed sorghum-soybean meal, low-protein diets

The dietary crude protein (CP) can be reduced by four percentage units when corn-soybean meal (SBM) and crystalline amino acids (AA) are used to formulate diets for growing pigs. With sorghum the results have not been conclusive. Therefore, two experiments were conducted to determine the lowest CP value in sorghum-SBM, AA supplemented diets, using plasma urea nitrogen (PUN), growth performance, and carcass characteristics as the response criteria. In Experiment 1, the percentage of CP in the treatments was as follows: T1) 16.0, control diet; T2) 14.5; T3) 13.0; and T4) 11.5. Eight gilts were used in a cross-over design with four periods of 7 days each. Blood samples were collected the last day of the period to determine PUN. Several regression models were used to obtain the best prediction of PUN. The lowest PUN indicated that CP can be reduced from 16 to 11.5%. The best regression model was the nonlineal exponential, which can predict that the minimum plasma urea concentration is obtained with 10.48% of CP. In Experiment 2, the percentage of CP and metabolizable energy Mcal kg-1) were as follows: T1) 16, 3.265, control; T2) 16, 3.165; T3) 14.5, 3.265; T4) 14.5, 3.165; T5) 11.5, 3.265; and T6) 11.5, 3.165. Thirty barrows were assigned in a completely randomized design with a 3 × 2 factorial arrangement, six treatments and five replicates of one barrow (individually penned) for each treatment. The lowest CP reduced the average daily gain, feed gain ratio, and PUN. The lowest ME reduced the feed gain ratio. These results indicate that reducing CP diminishes PUN, although some productive variables are affected.

La proteína cruda (PC) puede reducirse cuatro unidades porcentuales cuando se utiliza maíz-pasta de soya y aminoácidos (AA) para cerdos en crecimiento. Con sorgo no se han obtenido resultados concluyentes. Por ello se realizaron dos experimentos para determinar el porcentaje mínimo de PC en dietas sorgo-pasta de soya adicionadas con AA, con base en la concentración de urea en plasma de cerdos en crecimiento, y su respuesta productiva. En el Experimento 1, el porcentaje de PC en los tratamientos fue: T1) testigo, 16.0; T2) 14.5; T3) 13.0; y T4) 11.5. Se utilizaron ocho cerdas en un diseño cruzado (cross-over), con cuatro periodos de siete días. El último día de cada periodo se obtuvo sangre para determinar la concentración de urea. Se utilizaron varios modelos de regresión para obtener el que mejor predijera la concentración de urea. El menor nivel de urea indicó que la proteína puede reducirse de 16% hasta 11.5%. El mejor modelo de regresión fue el no lineal exponencial, con el que se puede predecir que la mínima concentración de urea se obtiene con 10.48% de PC. En el Experimento 2, los porcentajes de PC y las Mcal EM kg-1 fueron: T1) testigo, 16, 3.265; T2) 16, 3.165; T3) 14.5, 3.265; T4) 14.5, 3.165; T5) 11.5, 3.265; y T6) 11.5, 3.165, que se proporcionaron a 30 cerdos machos castrados (cinco repeticiones por tratamiento), alojados individualmente, en un diseño completamente al azar con arreglo factorial 3 × 2, con seis tratamientos y cinco repeticiones por tratamiento. La menor PC redujo la GDP, la conversión alimenticia y la urea en plasma. La menor EM redujo la conversión alimenticia. Lo anterior indica que disminuir la proteína reduce la urea en plasma, aunque se afectan algunas variables productivas.

Lysine requirement of starting barrows from two genetic groups fed on low crude protein diets

A trial was carried out to determine the lysine requirement for starting barrows fed on ideal protein concept-based diets. Thirty-two pigs from a commercial crossbred genetic group (CCGG, BW=15.9 ± 1.4 kg) and 32 pigs from a dam line one (DLGG, BW= 14.8 ± 1.0 kg) were used. Pigs were allotted to 4 treatments with diets containing increasing levels of total lysine (0.80, 1.00, 1.20 and 1.40 percent). Methionine+cystine, threonine and tryptophan were adjusted according to ideal protein profile. Data from performance, plasma urea nitrogen (PUN) and carcass composition were analyzed. CCGG showed higher daily feed intake, daily weight gain, PUN and protein:fat ratio in carcass, while DLGG showed higher fat carcass content and nitrogen retention. Fat content and protein:fat ratio in carcass for CCCGG and PUN and crude protein carcass content for DLGG showed quadratic response to increasing total lysine levels. Derivations of the quadratic equations indicated the total lysine requirement for CCGG starting barrows is 1.15 percent and for DLGG starting barrows is 1.09 percent.

Foi realizado um trabalho com o objetivo de determinar a exigência em lisina para suínos castrados em fase inicial, alimentados com dietas formuladas de acordo com o conceito de proteína ideal. Trinta e dois suínos provenientes de cruzamento comercial (CC, PV = 15,9 kg) e 32 suínos provenientes de linhagem materna (LM, PV= 14,8 kg) foram alimentados com quatro dietas contendo níveis crescentes de lisina total (0,80; 1,00; 1,20 e 1,40 por cento). Metionina + cistina, triptofano e treonina foram adicionados às dietas para manter constante o padrão de proteína ideal. Foram analisados dados de desempenho, nitrogênio da uréia plasmática (NUP) e carcaça. Suínos do grupo CC apresentaram maior consumo diário de ração, ganho diário de peso, NUP e relação proteína: gordura na carcaça, enquanto que os animais do grupo LM apresentaram maiores teores de gordura na carcaça e retenção de nitrogênio. Teor de gordura e relação proteína:gordura na carcaça para o grupo CC e NUP e teor de proteína bruta na carcaça para o grupo LM apresentaram resposta quadrática aos níveis de lisina. Derivações das equações indicaram a exigência de lisina total de suínos machos castrados do grupo cruzamento industrial de 1,15 por cento e do grupo linhagem materna de 1,09 por cento.

THE CLINICAL STUDY OF 17-L-CRYSTALLINE AMINO ACIDS SOLUTION IN PATIENTS WITH CARCINOMA OF ESOPHAGUS AND CARDIA AFTER RESECTION / 营养学报

Protein synthesis and nitrogen equilibrium have been investigated in the early postoperative period in 21 patients with carcinoma of esophagus and cardia after resection. They received 17-L-crystalline amino acid (AAM) and glucose infusion through parenteral route and were compared with a series' of 11 patients taking similar volume and calorie with protein hydrolysate (PRH) and glucose.In AAM series, the plasma protein could be maintained in preoperatiye level but not in PRH series. In AAM series a nitrogen equilibrium was reached while in PRH series was a negative equilibrium. The cellular immunity and serum amino acid spectrum were improved or replaced in both series, but they were better in AAM series. The improvement in nitrogen balance was probably mainly due to an increase in protein synthesis.