Fast backward steps and detachment of single kinesin molecules measured under a wide range of loads.

To understand force generation under a wide range of loads, the stepping of single kinesin molecules was measured at loads from -20 to 42 pN by optical tweezers with high temporal resolution. The optical trap has been improved to halve positional noise and increase bandwidth by using 200-nm beads. The step size of the forward and backward steps was 8.2 nm even over a wide range of loads. Histograms of the dwell times of backward steps and detachment fit well to two independent exponential equations with fast (~0.4 ms) and slow (>3 ms) time constants, indicating the existence of a fast step in addition to the conventional slow step. The dwell times of the fast steps were almost independent of the load and ATP concentration, while those of the slow backward steps and detachment depended on those. We constructed the kinetic model to explain the fast and slow steps under a wide range of loads.

In vivo axial load-share ratio measurement using a novel hexapod system for safe external fixator removal.

BACKGROUND: External fixation is widely used in the treatment of traumatic fractures; however, orthopedic surgeons encounter challenges in deciding the optimal time for fixator removal. The axial load-share ratio (LS) of the fixator is a quantitative index to evaluate the stiffness of callus healing. This paper introduces an innovative method for measuring the LS and assesses the method's feasibility and efficacy. Based on a novel hexapod LS-measurement system, the proposed method is to improve the convenience and precision of measuring LS in vivo, hence facilitating the safe removal of external fixators. METHODS: A novel hexapod system is introduced, including its composition, theoretical model, and method for LS measurement. We conducted a retrospective study on 82 patients with tibial fractures treated by the Taylor Spatial Frame in our hospital from September 2018 to June 2020, of which 35 took LS measurements with our novel method (Group I), and 47 were with the traditional method (Group II). The external fixator was removed when the measurement outcome (LS < 10%) was consistent with the surgeon's diagnosis based on the clinical and radiological assessment (bone union achieved). RESULTS: No significant difference was found in the fracture healing time (mean 25.3 weeks vs. 24.9 weeks, P > 0.05), frame-wearing duration (mean 25.5 weeks vs. 25.8 weeks, P > 0.05), or LS measurement frequency (mean 1.1 times vs. 1.2 times, P > 0.05). The measurement system installation time in Group I was significantly shorter compared to Group II (mean 14.8 min vs. 81.3 min, P < 0.001). The LS value of the first measurement in Group I was lower than that of Group II (mean 5.1% vs. 6.9%, P = 0.011). In Group I, the refracture rate was 0, but in Group II it was 4.3% (2/47, P > 0.05). CONCLUSION: The novel hexapod LS-measurement system and involved method demonstrated enhanced convenience and precision in measuring the LS of the external fixator in vivo. The LS measurement indicates the callus stiffness of fracture healing, and is applicable to evaluate the safety of removing the fixator. Consequently, it is highly recommended for widespread adoption in clinical practice.

A Prospective Study on a Suture Force Feedback Device for Training and Evaluating Junior Surgeons in Anastomotic Surgical Closure.

BACKGROUND: Surgical reconstruction is a crucial stage in various surgeries, including pancreaticoduodenectomy, as it can significantly affect the surgical results. The objective was to design a suture force feedback (SFF) device that can precisely measure the suture force during surgical closures. Afterward, the device was used to train junior surgeons in surgical closure techniques. METHODS: The SFF was used to capture the suture force data of experienced surgeons. This data was utilized to train and assess junior surgeons. The SFF device had 2 tactile-based force sensors that measured the applied force. Whenever the applied force was not within the optimal force range, the device provided feedback to the surgeon. A workshop was conducted to train junior surgeons in surgical closure techniques to improve their suturing skills. RESULTS: Thirty-seven junior surgeons were enrolled in this training, of whom only 24 completed the 30-day training program. The pre-assessment results revealed that the force exerted by junior surgeons during suture knot-tying was uneven compared with that of the experienced surgeons, with a significant difference in the force exerted per knot throw (P = 0.005. Before the training program, junior surgeons applied a force of 3.89 ± 0.43 N, which was more than twice the force applied by experienced surgeons (1.75 ± 0.12 N). However, after completing the 30-day training program, their force improved to 2.35 ± 0.13 N. CONCLUSIONS: The SFF device was shown to be an encouraging training tool for improving the surgical closure dexterity and technique of the participating junior surgeons.

Application of atomic force microscopy in the characterization of fruits and vegetables and associated substances toward improvement in quality, preservation, and processing: nanoscale structure and mechanics perspectives.

Fruits and vegetables are essential horticultural crops for humans. The quality of fruits and vegetables is critical in determining their nutritional value and edibility, which are decisive to their commercial value. Besides, it is also important to understand the changes in key substances involved in the preservation and processing of fruits and vegetables. Atomic force microscopy (AFM), a powerful technique for investigating biological surfaces, has been widely used to characterize the quality of fruits and vegetables and the substances involved in their preservation and processing from the perspective of nanoscale structure and mechanics. This review summarizes the applications of AFM to investigate the texture, appearance, and nutrients of fruits and vegetables based on structural imaging and force measurements. Additionally, the review highlights the application of AFM in characterizing the morphological and mechanical properties of nanomaterials involved in preserving and processing fruits and vegetables, including films and coatings for preservation, bioactive compounds for processing purposes, nanofiltration membrane for concentration, and nanoencapsulation for delivery of bioactive compounds. Furthermore, the strengths and weaknesses of AFM for characterizing the quality of fruits and vegetables and the substances involved in their preservation and processing are examined, followed by a discussion on the prospects of AFM in this field.

Molecular Insights into the Interaction Mechanism Underlying the Aggregation of Humic Acid and Its Adsorption on Clay Minerals.

Humic acid (HA) is ubiquitous in both terrestrial and aquatic environments, and understanding the molecular interaction mechanisms underlying its aggregation and adsorption is of vital significance. However, the intermolecular interactions of HA-HA and HA-clay mineral systems in complex aqueous environments remain elusive. Herein, the interactions of HA with various model surfaces (i.e., HA, mica, and talc) were quantitatively measured in aqueous media at the nanoscale using an atomic force microscope. The HA-HA interaction was found to be purely repulsive during surface approach, consistent with free energy calculation; during retraction, pH-dependent adhesion was observed due to the protonation/deprotonation of HA that influences the formation of hydrogen bonds. Different from the mica case, hydrophobic interaction was detected for the HA-talc system at pH 5.8, contributing to the stronger HA-talc adhesion, as also evidenced by adsorption results. Notably, HA-mica adhesion strongly depended on the loading force and contact time, most likely because of the short-range and time-dependent interfacial hydrogen bonding interaction under confinement, as compared to the dominant hydrophobic interaction for the HA-talc case. This study provides quantitative insights into the fundamental molecular interaction mechanisms underlying the aggregation of HA and its adsorption on clay minerals of varying hydrophobicity in environmental processes.

Novel Straightening-Machine Design with Integrated Force Measurement for Straightening of High-Strength Flat Wire.

In a punch-bending machine, wire products are manufactured for a wide range of industrial sectors, such as the electronics industry. The raw material for this process is flat wire made of high-strength steel. During the manufacturing process of the flat wire, residual stresses and plastic deformations are induced into the wire. These residual stresses and deformations fluctuate over the length of the semi-finished product and have a negative effect on the final product quality. Straightening machines are used to reduce this influence to a minimum. So far, the adjustment of a straightening machine has been performed manually, which is a lengthy and complex task even for an experienced worker. This inevitably leads to the use of inefficient straightening strategies and causes high rejection rates in the entire production process. Due to a lack of sensor information from the straightening operation, application of modern feedback control methods has not been practicable. This paper presents a novel design for a straightening machine with an integrated, precise straightening force measurement. By simultaneously monitoring the position of the straightening rollers, state variables of the straightening operation can be derived. Additionally, a tension control for feeding the flat wire is introduced. This is implemented to mitigate the disturbing effects caused by irregularities in the wire-feeding process. In the results of this article, the high precision of the developed force measurement design and its possible applications are shown.

A Laboratory Device Designed to Detect and Measure the Resistance Force of a Diagonal Conveyor Belt Plough.

This paper presents a laboratory device simulating a section of a conveyor belt on which a diagonal plough is installed. Experimental measurements were carried out in a laboratory belonging to the Department of Machine and Industrial Design at the VSB-Technical University of Ostrava. During the measurements, a plastic storage box, representing a piece load, transported on the surface of a conveyor belt at a constant speed was brought into contact with the front surface of a diagonal conveyor belt plough. The aim of this paper is to determine the amount of resistance generated by the diagonal conveyor belt plough when it is placed at different angles of inclination ß [deg] in relation to the longitudinal axis, based on the experimental measurements performed using a laboratory measuring device. Based on the measured values of tensile force required to keep the conveyor belt moving at a constant speed, the resistance to the conveyor belt movement is expressed, with a value of 20.8 ± 0.3 N being attained. Based on the ratio of the measured value of the arithmetic average of the resistance force and the weight of the used length of the conveyor belt, a mean value of the specific movement resistance of the size 0.33 [N·N - 1] is calculated. This paper presents the time records obtained by measuring the tensile forces, on the basis of which it is possible to determine the magnitude of the force. The resistance during the ploughing operation of the diagonal plough when acting on a piece load placed on the working surface of the conveyor belt is presented. From the measured values of tensile forces presented in the tables, this paper reports the calculated values of the friction coefficient obtained during the movement of the diagonal plough when moving a piece of load with the defined weight from the working surface of the relevant conveyor belt. The maximum value of the arithmetic mean for the friction coefficient in motion µ = 0.86 was measured at an inclination angle of the diagonal plough of ß = 30 deg.

A Novel Ultrasound Robot with Force/torque Measurement and Control for Safe and Efficient Scanning.

Medical ultrasound is of increasing importance in medical diagnosis and intraoperative assistance and possesses great potential advantages when integrated with robotics. However, some concerns, including the operation efficiency, operation safety, image quality, and comfort of patients, remain after introducing robotics into medical ultrasound. In this paper, an ultrasound robot integrating a force control mechanism, force/torque measurement mechanism, and online adjustment method, is proposed to overcome the current limitations. The ultrasound robot can measure operating forces and torques, provide adjustable constant operating forces, eliminate great operating forces introduced by accidental operations, and achieve various scanning depths based on clinical requirements. The proposed ultrasound robot would potentially facilitate sonographers to find the targets quickly, improve operation safety and efficiency, and decrease patients' discomfort. Simulations and experiments were carried out to evaluate the performance of the ultrasound robot. Experimental results show that the proposed ultrasound robot is able to detect operating force in the z-direction and torques around the x- and y- directions with errors of 3.53% F.S., 6.68% F.S., and 6.11% F.S., respectively, maintain the constant operating force with errors of less than 0.57N, and achieve various scanning depths for target searching and imaging. This proposed ultrasound robot has good performance and would potentially be used in medical ultrasound.

Comprehensive Indicators for Evaluating and Seeking Elasto-Magnetic Parameters for High-Performance Cable Force Monitoring.

The elasto-magnetic method is a promising pathway for cable force monitoring in cable-stayed bridges. Under the action of an externally applied pulsed magnetic field, both the variation in the main flux recorded by the induction coil and the localized surface magnetic field measured by the packaged magnetic sensor are typical signals for observing the elasto-magnetic effect in tensioned cables. However, the performances of the parameters extracted from the two types of elasto-magnetic signals are never strictly compared in the experiment. Meanwhile, comprehensive indicators for evaluating the ability of elasto-magnetic parameters on cable force characterization are seldom discussed. As a result, it is difficult to compare the performances of elasto-magnetic devices developed by different teams, and the pathway of seeking new parameters for cable force monitoring is obstructed. In this study, elasto-magnetic calibration experiments were performed on a cable of seven-wire steel strands to simultaneously measure the variation in the main flux and the localized surface magnetic field. Comprehensive indicators considering sensitivity, hysteresis error, and cable force resolution are proposed to examine the performances of classic elasto-magnetic parameters and new candidate ones. Through comparative study, two new parameters demonstrated outstanding ability for cable force measurement, and they are the minimum amplitude of the induced voltage and the area under the curve between two points of 3 dB height of the voltage measured by a Hall sensor. The latter is recommended for high-performance cable force monitoring from the perspective of simplicity in sensor configuration.

HiVTac: A High-Speed Vision-Based Tactile Sensor for Precise and Real-Time Force Reconstruction with Fewer Markers.

Although they have been under development for years and are attracting a lot of attention, vision-based tactile sensors still have common defects-the use of such devices to infer the direction of external forces is poorly investigated, and the operating frequency is too low for them to be applied in practical scenarios. Moreover, discussion of the deformation of elastomers used in vision-based tactile sensors remains insufficient. This research focuses on analyzing the deformation of a thin elastic layer on a vision-based tactile sensor by establishing a simplified deformation model, which is cross-validated using the finite element method. Further, this model suggests a reduction in the number of markers required by a vision-based tactile sensor. In subsequent testing, a prototype HiVTac is fabricated, and it demonstrates superior accuracy to its vision-based tactile sensor counterparts in reconstructing an external force. The average error of inferring the direction of external force is 0.32∘, and the root mean squared error of inferring the magnitude of the external force is 0.0098 N. The prototype was capable of working at a sampling rate of 100 Hz and a processing frequency of 1.3 kHz, even on a general PC, allowing for real-time reconstructions of not only the direction but also the magnitude of an external force.

Design and Development of a Tri-Axial Turning Dynamometer Utilizing Cross-Beam Type Force Transducer for Fine-Turning Cutting Force Measurement.

The main focus of this work was the design and development of a cross-beam force transducer for use in the construction of a tri-axial dynamometer. This dynamometer would be able to measure the cutting force along all three axes simultaneously during turning operations. The force transducer was built on the concept of the Maltese cross-beam, but it had been modified and improved so that it had a higher sensitivity and reduced the amount of interference error or cross-talk error that it produced. An investigation into the distribution of strain, as well as the determination of sensor locations within the transducer construction was carried out by means of finite element analysis. In order to develop a prototype of a turning dynamometer, a number of piezoresistive strain gauges were utilized in the transducer. In order to determine sensitivity, linearity, hysteresis, and repeatability, calibration tests were performed in three directions that were perpendicular to one another. To investigate the dynamic properties and capabilities of the dynamometer for use in turning applications, both modal analysis and actual turning tests were performed. The results of the experiments demonstrated that the newly developed turning dynamometer is a realistic approach for measuring cutting force in machining without reliability and accuracy.

Silicon Cantilever for Micro/Nanoforce and Stiffness Calibration.

The paper deals with cantilevers made from monocrystalline silicon by processes of microtechnology. The cantilevers are passive structures and have no transducers. The application as a material measure for the inspection of stylus forces is in the center of investigations. A simple method is the measurement of the deflection of the cantilever at the position of load by the force if the stiffness of the cantilever at this position is known. Measurements of force-deflection characteristics are described and discussed in context with the classical theory of elastic bending. The methods of determining the stiffness are discussed together with results. Finally, other methods based on tactile measurements along the cantilever are described and tested. The paper discusses comprehensively the properties of concrete silicon chips with cantilevers to underpin its applicability in industrial metrology. The progress consists of the estimation of the accuracy of the proposed method of stylus force measurement and the extraction of information from a tactile measured profile along the silicon cantilever. Furthermore, improvements are proposed for approaches to an ideal cantilever.

Strain Gauge Measuring System for Subsensory Micromotions Analysis as an Element of a Hybrid Human-Machine Interface.

The human central nervous system is the integrative basis for the functioning of the organism. The basis of such integration is provided by the fact that the same neurons are involved in various sets of sensory, cognitive, and motor functions. Therefore, the analysis of one set of integrative system components makes it possible to draw conclusions about the state and efficiency of the other components. Thus, to evaluate a person's cognitive properties, we can assess their involuntary motor acts, i.e., a person's subsensory reactions. To measure the parameters of involuntary motor acts, we have developed a strain gauge measuring system. This system provides measurement and estimation of the parameters of involuntary movements against the background of voluntary isometric efforts. The article presents the architecture of the system and shows the organization of the primary signal processing in analog form, in particular the separation of the signal taken from the strain-gauge sensor into frequency and smoothly varying components by averaging and subtracting the analog signals. This transfer to analog form simplifies the implementation of the digital part of the measuring system and allowed for minimizing the response time of the system while displaying the isometric forces in the visual feedback channel. The article describes the realization of the system elements and shows the results of its experimental research.

Design and Shape Optimization of Strain Gauge Load Cell for Axial Force Measurement for Test Benches.

The load cell is an indispensable component of many engineering machinery and industrial automation for measuring and sensing force and torque. This paper describes the design and analysis of the strain gauge load cell, from the conceptional design stage to shape optimization (based on the finite element method (FEM) technique) and calibration, providing ample load capacity with low-cost material (aluminum 6061) and highly accurate force measurement. The amplifier circuit of the half Wheatstone bridge configuration with two strain gauges was implemented experimentally with an actual load cell prototype. The calibration test was conducted to evaluate the load cell characteristics and derive the governing equation for sensing the unknown load depending on the measured output voltage. The measured sensitivity of the load cell is approximately 15 mV/N and 446.8 µV/V at a maximum applied load of 30 kg. The findings are supported by FEM results and experiments with an acceptable percentage of errors, which revealed an overall error of 6% in the worst situation. Therefore, the proposed load cell meets the design considerations for axial force measurement for the laboratory test bench, which has a light weight of 20 g and a maximum axial force capacity of 300 N with good sensor characteristics.

Interfacial Adhesion between Fatty Acid Collectors and Hydrophilic Surfaces: Implications for Low-Rank Coal Flotation.

Fatty acids, which are enriched in vegetable oil, have attracted much attention in low-rank coal flotation because of their unique chemical structure. In this study, density functional theory calculations, molecular dynamics simulations, and atomic force microscopy were employed to investigate the adsorption structure and forces between collectors and hydrophilic surfaces. The results show that fatty acids can be easily adsorbed onto surfaces through hydrogen bonds, and can cover the oxygen sites. The existence of hydration film on hydrophilic surfaces prevented nonpolar molecules from being able to adsorb, while polar fatty acids could adsorb and expel water molecules. The adhesion force between the RCOOH-terminated probe and the surface appeared in the retraction process, which differed significantly from that of the RCH3-terminated probe, indicating that polar fatty acids are more suitable as flotation collectors for low-rank coal than nonpolar hydrocarbon oil. The simulation and AFM test revealed the mechanisms of polar fatty acids, and can provide guidance for low-rank coal flotation applications.

Establishment, FEM analysis and experimental validation of tooth movement prediction model of orthodontic archwire T-loop.

BACKGROUND: The T-loop has been used clinically to close gap between teeth. And it is a typical orthodontic archwire bending method. However, the design of the T-loop parameters for different patients is based on the clinical experience of the dentists. The variation in dentists' clinical experience is the main reason for inadequate orthodontic treatment, even high incidence of postoperative complications. METHODS: Firstly, the tooth movement prediction model is established based on the analysis of the T-loop structure and the waxy model dynamic resistance. As well as the reverse reconstruction of the complete maxillary 3D model based on the patient CBCT images, the oral biomechanical FEM analysis is completed. A maxillary waxy dental model is manufactured to realize the water-bath measurement experiment in vitro mimicking the oral bio-environment. Thus, the calculated, simulation and experimental data are obtained, as well as obtaining a cloud of total deformation from the simulation analysis. RESULTS: The growth trend of the 11 sets of simulation data is the same as that of the experimental data. And all of them show that the tooth displacement is positively correlated with the cross-sectional size of the archwire, and the clearance distance. As well as the higher Young's modulus of the archwire material, the greater the tooth displacement. And the effect of archwire parameters on tooth displacement derived from simulation and experimental data is consistent with the prediction model. The experimental and calculated data are also compared and analyzed, and the two kinds of data are basically consistent in terms of growth trends and fluctuations, with deviation rates ranging from 2.17 to 10.00%. CONCLUSIONS: This study shows that the accuracy and reliability of the tooth movement prediction model can be verified through the comparative analysis and deviation calculation of the obtained calculated, simulation and experimental data, which can assist dentists to safely and efficiently perform orthodontic treatment on patients. And the FEM analysis can achieve predictability of orthodontic treatment results.

New device with force sensors for laparoscopic liver resection - investigation of grip force and histological damage.

INTRODUCTION: As the benefits of minimally invasive surgery are recognized, the rate of laparoscopic liver resection (LLR) is rapidly increasing. Liver tissue is fragile compared to tissue of the stomach and colon. In endoscopic and robotic surgery, sufficient tactile sensation is yet to be obtained. Therefore, it is necessary to measure and indicate the grip force of forceps during surgery. We developed a new device consisting of force sensors and investigated its grip force and the resulting histological damage to liver tissue. MATERIAL AND METHODS: We measured the grip force generated during laparoscopic surgery in pigs using the forceps with pressure sensors developed by us. Throughout the hepatectomy, we measured the grip force generated by the forceps in real time. We investigated the histological damage to the liver caused by using the forceps with different grip forces. RESULTS: The subject produced a mean grip force of 1.75 N during the procedures. The maximum grip force was 3.38 N. By grasping the tissues of the liver with forceps, bleeding and destruction of the hepatic lobules were observed in a manner dependent on increasing grip force. CONCLUSION: The new device is necessary for preventing liver damage in laparoscopic hepatic resection.

Whole-body vibration training versus conventional balance training in patients with severe COPD-a randomized, controlled trial.

BACKGROUND: Whole-body vibration training (WBV) performed on a vibration platform can significantly improve physical performance in patients with chronic obstructive pulmonary disease. It has been suggested that an important mechanism of this improvement is based on an improvement in balance. Therefore, the aim of this study was to investigate the effects of WBV compared to conventional balance training. METHODS: 48 patients with severe COPD (FEV1: 37 ± 7%predicted) and low exercise performance (6 min walk distance (6MWD): 55 ± 10%predicted) were included in this randomized controlled trial during a 3 week inpatient pulmonary rehabilitation. All patients completed a standardized endurance and strength training program. Additionally, patients performed 4 different balance exercises 3x/week for 2 sets of 1 min each, either on a vibration platform (Galileo) at varying frequencies (5-26 Hz) (WBV) or on a conventional balance board (BAL). The primary outcome parameter was the change in balance performance during a semi tandem stance with closed eyes assessed on a force measurement platform. Muscular power during a countermovement jump, the 6MWD, and 4 m gait speed test (4MGST) were secondary outcomes. Non-parametric tests were used for statistical analyses. RESULTS: Static balance performance improved significantly more (p = 0.032) in favor of WBV (path length during semi-tandem stand: - 168 ± 231 mm vs. + 1 ± 234 mm). Muscular power also increased significantly more (p = 0.001) in the WBV group (+ 2.3 ± 2.5 W/kg vs. - 0.1 ± 2.0 W/kg). 6MWD improved to a similar extent in both groups (WBV: 48 ± 46 m, p < 0.001 vs. BAL: 38 ± 32 m; p < 0.001) whereas the 4MGST increased significantly only in the WBV-group (0.08 ± 0.14 m/s2, p = 0.018 vs. 0.01 ± 0.11 m/s2, p = 0.71). CONCLUSIONS: WBV can improve balance performance and muscular power significantly more compared to conventional balance training. TRIAL REGISTRATION: Clinical-Trials registration number: NCT03157986; date of registration: May 17, 2017. https://clinicaltrials.gov/ct2/results?cond=&term=NCT03157986&cntry=&state=&city=&dist = .

Comparison of bandaging techniques to prevent cochlear implant magnet displacement following MRI.

INTRODUCTION: For cochlear implants (CI) with removable magnets, a pressure bandage usually is recommended during MR imaging to avoid magnet dislocation. Nevertheless, this complication is regularly observed despite applying a pressure bandage. The aim of this study was to compare various bandaging techniques to avoid magnet displacement. MATERIALS AND METHODS: As an experimental model a force measuring stand was developed and validated, on which the process of magnet dislocation could be simulated on a cochlear implant. In a test series with six combinations of cohesive and elastic bandages with different counter pressure elements (CPE), the forces required to induce magnet dislocation against the resistance of a compression bandage was determined. In addition, the inter- and intraindividual variability of the compression bandages was measured for ten different users. RESULTS: The cohesive bandage had the lowest average holding force of 10.70 N. The elastic bandage developed more than four times the retention force of the cohesive bandage (44.88 N, p < 0.01). By adding a CPE, these values could be increased highly significantly up to factor 3. The optimum combination in terms of fixation force against magnet dislocation was an elastic bandage plus a cylindrical CPE (76.60 N). The data showed a high interindividual variability. CONCLUSION: Even though most CI manufacturers now offer 3T-conditional implants, a pressure bandage will have to be applied to thousands of patients with previous implant generations to prevent magnet dislocation. We examined for the first time force measurements to compare different bandaging techniques by detecting the holding force of the CI magnet. We were able to identify an optimized combination of a bandage and a CPE to immobilize the CI magnet. However, our data also demonstrated a significant scatter amongst different examiners. Although our data provide valuable data for potential clinical application, future development of the dressing technique is required for human use.

Module for Monitoring the Probe-Skin Contact Force in the Study of Vibration Perception on the Wrist.

This paper presents a module for monitoring the contact force between a probe for measuring vibration perception on the wrist and the skin. The module was designed for an original measuring stand for the automatic testing of the vibrotactile discrimination thresholds using the psychophysical adaptive method of 1 up-2 down with two or three interval forced choices (2IFC, 3IFC). Measurement methods were implemented in LabVIEW software. The inspiration for the project was the need to check the possibility of building a vibrating interface for transmitting information through vibrations delivered to the wrist via a bracelet. The test procedure on the wrist is not standardized; however, during its development, the recommendations of the Polish Norm-International Organization for Standardization PN-ISO 13091-1, 2006 were adopted. This standard contains methods for measuring vibration sensation thresholds on the fingertips for the assessment of neural dysfunction. The key to the repeatability of measurements seems to be the ability to continuously control the pressure of the measuring probe on the skin. This article compares two solutions for measuring the contact force along with an analysis of their accuracy and the impact of vibrations on the measured values. Moreover, the results of measurements of vibrotactile amplitude and frequency discrimination thresholds obtained on the ventral wrist at five frequencies (25, 32, 63, 125 and 250 Hz) are presented.