Spatial, temporal, and biological factors influencing plant responses to deicing salt in roadside bioinfiltration basins.

Plants are arguably the most visible components of stormwater bioretention basins and play key roles in stabilizing soils and removing water through transpiration. In regions with cold winters, bioretention basins along roadways can receive considerable quantities of deicing salt, much of which migrates out of the systems prior to the onset of plant growth but the rest remains in the soil. The resulting effects on plants presumably vary with time (due to annual weather patterns), space (because stormwater exposure is location-dependent), and biology (because plant taxa differ in their salt tolerance). The goal of this study was to investigate the magnitude of deicing salt's effects on bioretention plants and how it varies with spatial, temporal, and biological factors. The study took place in a set of five bioretention basins in Philadelphia, USA that receive runoff from a major highway. Over a five-year period, the electrical conductivity (EC) of influent stormwater frequently exceeded 1 mS cm-1 in winter, and occasionally surpassed that of seawater (∼50 mS cm-1). In both of the years when soil EC was measured as well, it remained elevated through all spring months, especially near basin inlets and centers. Mortality of nine plant taxa ranged widely after three years (0-90%), with rankings largely corresponding to salt tolerances. Moreover, leaf areas and/or crown volumes were strongly reduced in proportion to stormwater exposure in seven of these taxa. In the three taxa evaluated for tissue concentrations of 14 potentially toxic elements (Hemerocallis 'Happy Returns', Iris 'Caesar's Brother', and Cornus sericea 'Cardinal'), only sodium consistently exceeded the toxicity limit for salt intolerant plants (500 mg kg-1). However, exceedance of the sodium toxicity limit was associated with plants' topographic positions, with median concentrations greatest in the bottom of basins and least on basin rims. This study demonstrates that deicing salts can have detrimental effects on plants in bioretention basins, with the strongest effects likely to occur in years with the greatest snowfall (and therefore deicing salt use), in portions of basins with greatest stormwater exposure (typically around inlets and centers), and in plants with minimal salinity tolerance. Our results therefore underscore the value of installing salt-tolerant taxa in basins likely to experience any frequency of deicing salt exposure.

Effects of Regional Climate and BMP Type on Stormwater Nutrient Concentrations in BMPs: A Meta-Analysis.

Nutrient treatment performance of stormwater best management practices (BMPs) is highly variable. Improved nutrient management with BMPs requires a better understanding of factors that influence stormwater BMP treatment processes. We conducted a meta-analysis of vegetated BMPs in the International Stormwater BMP Database and compared influent and effluent nitrogen and phosphorus concentrations to quantify the BMP effect on nutrient management across climates. BMP effect on nutrient concentration change was compared between vegetated BMPs in wet and dry climates. We examined paired dissolved inorganic nitrogen (DIN), total nitrogen (TN), dissolved inorganic phosphorus (DIP), total phosphorus (TP), and combinations of these analytes as dissolved inorganic ratios and N:P ratios. Meta-analysis with subgroup analysis was used to determine differences between wet and dry climates and among vegetated BMP types. We found that across both wet and dry climates, BMPs leach DIP and TP, increase the fraction of dissolved inorganic P (DIP:TP), and decrease dissolved N:P ratios. Dry-climate BMPs leach DIP and TP more consistently and at a higher magnitude than wet-climate BMPs, and bioretention leaches more DIP than grass strips and swales. These findings generally align with biogeochemical cycling, differences in influent chemistry, and BMP design types and goals.

Opportunities and challenges in green stormwater infrastructure (GSI): A comprehensive and bibliometric review of ecosystem services from 2000 to 2021.

The great challenges induced by global climate change coupled with rapid urbanization underline the growing urgency for a change in stormwater management with a novel integrated approach. This study conducted a comprehensive review on state-of-the-art knowledge in the research field of green storm infrastructure (GSI) using bibliometric analysis. A corpus of 3988 GSI-related publications (2000-2021) extracted from the Web of Science database was used to evaluate the scientific output in GSI research through the "Bibliometrix" R package and "CiteSpace". Ever since 2010, the number of publications per year exhibited an exponential increase, with the annual publication growth rate of 28.61%. Notably, the United States (23.55%) and China (19.58%) contributed most in GSI publications. "Water" (306) was identified as the most relevant journal in GIS research field, followed by "Sustainability" (252) and "Science of the Total Environment" (200). Cluster analysis unveiled the predominant research themes, i.e., "Conceptual development of GSI" (69.25%), "Adaptation of GSI" (46.89%), and "Performance evaluation of GSI practices" (18.28%). Research foci have generally shifted from conventional engineering-based frameworks (e.g., reduce stormwater runoff and enhance water quality) to ecological-based multi-elements (e.g., preserve natural resources, augment urban biodiversity and optimize land-use patterns). This systematic review concludes trends, challenges and future research prospects of GSI, and aims to provide reference and guidance for decision-makers on the development of a more dynamic, resilient, and robust integrated GSI approach for sustainable urban stormwater management.

Addressing the social barriers to green stormwater infrastructure in residential areas from a socio-ecological perspective.

Despite the well-recognized financial limitations, social aspects can also impact the adoption of green stormwater infrastructure (GSI) as it is ingrained in the socio-ecological system we live in. Thus, this work focuses on gaining an understanding of the public's perceptions of GSI by considering cognitive biases that hinder its adoption. This work is composed of two forms of human-subject studies, including an online-based survey and a series of semi-structured interviews. The survey (n = 510) was conducted to gauge public opinions toward GSI, whereas the interviews with representatives of major local regulatory agencies were to learn about the logistics for GSI implementation in Mecklenburg County, NC. The results were interpreted using the theory of planned behavior of rational actors. Statistical results showed a weak interpretation through this theory to explain the survey participants' intention to adopt GSI measures. This could suggest that the incorporation of irrationality, such as cognitive biases, could further enhance the predictability of the theory. At the same time, an inconsistency between the findings from the survey and the interviews was identified: most survey participants showed an overall uniform positive attitude, intention, and behavior regarding GSI practice adoption, whereas the interviewed experts all suggested a wide diversity on such terms. Suggestions were made based on the findings for better policy-making on public engagement for local regulatory agencies. This research aims to help local stormwater management authorities explore shortcomings in current stakeholder engagement plans to gain sustainable support for GSI implementation in urbanized areas.

Ecosystem Benefits Provision of Green Stormwater Infrastructure in Chinese Sponge Cities.

The Sponge City Development (SCD) concept was initiated in 2012 to address severe urban flooding and water quality challenges in China. Green stormwater infrastructure (GSI) such as rain gardens have been adopted as critical stormwater management tools. Existing GSI research has focused primarily on their environmental performance, overlooking the human dimensions. The co-benefits of GSI have been particularly underinvestigated. We used social surveys (n = 607) and expert interviews (n = 11) to explore public perception of SCD and GSI in four pilot sponge cities, examining flood experience, stormwater concerns, GSI familiarity, institutional trust, and GSI benefit perception. The survey found high exposure to flooding, medium GSI familiarity, and strong institutional trust. The public showed greater concern on stormwater impacts on their quality-of-life than the water environment, rating the less-intended aesthetic and health values as the best-perceived benefits. Experience, familiarity, concern, trust, age, and city significantly affected GSI benefit perception. In contrast, the experts spoke more positively about the environmental benefits while indicating the inadequacy of public participation. The case of GSI in SCD offers broad implications for environmental governance and expert-public relationships in an era of rapid social, technological, and environmental change. Refining policies and regulations to incorporate social goals, bringing the public into the SCD process, and building up the GSI industry's capacity in planning, design, construction, and maintenance are critical to enhancing GSI benefits provision. Adopting the co-benefits approach will be essential to utilizing GSI as a place-making tool to create more sustainable and livable communities.

Valuing stakeholder preferences for environmental benefits of stormwater ponds: Evidence from choice experiment.

With population growth driving urban expansion in many cities in the United States, there is a need for a sustainable way to manage stormwater. Green stormwater infrastructure (GSI) is considered an innovative way to handle stormwater because of its potential to provide multiple ecosystem services (ES) beyond flooding reduction. However, there is limited research regarding the society's perceived value for GSI practices' co-benefits. This study utilized stated-preference data obtained from a choice experiment in an online survey of 1159 South Carolina (SC) residents to estimate a monetary value for the ES provided by wet detention ponds- the most widely adopted stormwater practice in coastal counties of SC. The benefits examined are flooding reduction, water quality, wildlife habitat, recreation, and scenic beauty. The data were analyzed using a Mixed logit formulation. Considering the differences across the state, the model was estimated separately for five counties. Findings indicate that residents are willing to pay $13.8 to $37.8 annually for a 50% improvement in pollutant removal efficiency of ponds in addition to their current stormwater fee. Also, they are willing to pay $12.5 to $42.9 per year for the nearest pond to have buffer vegetation and wildlife. They are also likely to pay $5 to $22.5 for ponds to contribute to their neighborhood's scenic beauty. Furthermore, the results indicate that respondents from three counties are willing to pay $5.4 to $13.2 for a 50% improvement in flooding reduction, while those from two counties are likely to pay $3.9 to $4.9 for ponds to have recreational benefits. The findings of the study could help stormwater managers in designing their stormwater management programs, especially for better evaluation of stormwater utility fees.

Resident Knowledge of and Engagement with Green Infrastructure in Toronto and Philadelphia.

Green infrastructure (GI) initiatives, including programs to plant trees and install bioswales, have been adopted by a growing number of local government and non-governmental organizations. While the details of these programs vary, a common characteristic of most Canadian and US GI initiatives is a distributed approach that includes both public and private land. To date, little research has explored residents' knowledge of GI or their engagement with related initiatives even though residents' installation of GI is often key to creating distributed GI networks. In this study, we (1) assess residents' knowledge of the term GI, (2) identify residents' level of engagement with GI initiatives, and (3) examine whether factors like level of concern about local environmental issues can predict GI knowledge or level of engagement with GI initiatives. We explored these objectives through a survey of residents in Toronto (Ontario, Canada) and Philadelphia (Pennsylvania, US). We found that about a quarter of survey respondents in both cities had previously heard the term "green infrastructure". Neither knowledge of GI nor level of engagement with GI initiatives could be predicted by the level of concern about local environmental issues, but residents' interest in using their outdoor space for nature activities (e.g., gardening) predicted GI knowledge in both cities and level of initiative engagement in Philadelphia. Our results suggest the need for widespread education campaigns that clearly define GI so that residents can be participants in policy discussions, link it with their needs, and identify ways to manage GI to create desired benefits.

Role of community participation for green stormwater infrastructure development.

The primary objective of this research is to understand the role of community participation in green stormwater infrastructure (GSI) development. There is a lack of understanding about the interactions of technical and non-technical factors that promote or hinder GSI implementation and development. This work uses the qualitative case study methodology to fulfil the objective and answer the research questions. The case study is based on the Proctor Creek Watershed, Atlanta Georgia, a rapidly growing urban area located in the southeastern United States. Data sources include participant interviews, documents, and field notes. Findings reveal that community participation in this case is embedded in collaborative partnership efforts. Also, social conditions highly influence the participation processes by dictating the priorities the community develops during participation processes. Factors such as funding and political support, promote green stormwater infrastructure implementation along with community participation. Additionally, community education addresses the challenge of resistance to change, hence community education plays a role in its development and implementation.

Integral stormwater management master plan and design in an ecological community.

Urban stormwater runoff nearly discharges directly into bodies of water through gray infrastructure in China, such as sewers, impermeable ditches, and pump stations. As urban flooding, water shortage, and other environment problems become serious, integrated water environment management is becoming increasingly complex and challenging. At more than 200ha, the Oriental Sun City community is a large retirement community located in the eastern side of Beijing. During the beginning of its construction, the project faced a series of serious water environment crises such as eutrophication, flood risk, water shortage, and high maintenance costs. To address these issues, an integral stormwater management master plan was developed based on the concept of low impact development (LID). A large number of LID and green stormwater infrastructure (GSI) approaches were designed and applied in the community to replace traditional stormwater drainage systems completely. These approaches mainly included bioretention (which captured nearly 85th percentile volume of the annual runoff in the site, nearly 5.4×10(5)m(3) annually), swales (which functioned as a substitute for traditional stormwater pipes), waterscapes, and stormwater wetlands. Finally, a stormwater system plan was proposed by integrating with the gray water system, landscape planning, an architectural master plan, and related consultations that supported the entire construction period. After more than 10 years of planning, designing, construction, and operation, Oriental Sun City has become one of the earliest modern large-scale LID communities in China. Moreover, the project not only addressed the crisis efficiently and effectively, but also yielded economic and ecological benefits.

Vertical distribution and migration of heavy metals in soil of green stormwater infrastructure receiving roof runoff.

In order to achieve effective management of urban stormwater runoff, green stormwater infrastructure (GSI) has been widely used worldwide. However, the problem of heavy metal contamination in GSI soils has gradually become a limiting factor for their development. In this paper, concentrations of 6 heavy metals were detected in soils from 0 to 80 cm depth in the GSI receiving roof runoff. The total concentration of the 6 heavy metals in topsoil of the GSI was 395 mg·kg-1, which was significantly higher than that of the control site (p < 0.01). Vertically, heavy metal concentrations in GSI soils decreased with depth. The concentrations of As, Cr, Cu, Pb and Zn in the soils at different depths (0-80 cm) of GSI were higher than those in the soils at the corresponding depths at the control site. This suggests that heavy metals may have migrated below 80 cm. The decrease in Zn/Cu ratios with depth indicated that Zn has a higher initial concentration and more of Zn accumulated in upper layer soil than Cu. Finally, we simulated the vertical migration of dissolved heavy metals in GSI soils by the Hydrus-1D model. Heavy metal concentrations in runoff and soil particle size distributions were examined, while adsorption isotherm experiments were conducted to determine input parameters for the model. The simulation results showed that only dissolved Zn would accumulate in the top soil layer. A short time of infiltration (<60 min) may have little effect on the profile shape of dissolved heavy metal levels in the soil. Substantial increases in infiltration may result in the migration of dissolved heavy metals to deeper depths in the soil.

Environmental drivers impact the accumulation and diversity of antibiotic resistance in green stormwater infrastructure.

Antibiotic resistance poses an urgent public health concern, with the environment playing a crucial role in the development and dissemination of resistant bacteria. There is a growing body of research indicating that stormwater is a significant source and transport vector of resistance elements. This research sought to characterize the role of green stormwater infrastructure (GSI), designed for stormwater infiltration, in accumulating and propagating antibiotic resistance in the urban water cycle. Sampling included 24 full-scale GSI systems representing three distinct types of GSI - bioswales, bioretention cells, and constructed wetlands. The results indicated that GSI soils accumulate antibiotic resistance genes (ARGs) at elevated concentrations compared to nonengineered soils. Bioretention cells specifically harbored higher abundances of ARGs, suggesting that the type of GSI influences ARG accumulation. Interestingly, ARG diversity in GSI soils was not impacted by the type of GSI design or the diversity of the microbial community and mobile genetic elements. Instead, environmental factors (catchment imperviousness, metals, nutrients, and salts) were identified as significant drivers of ARG diversity. These findings highlight how environmental selective pressures in GSI promote ARG persistence and proliferation independently of the microbial community. Therefore, GSI systems have the potential to be a substantial contributor of abundant and diverse ARGs to the urban water cycle.

Bioretention filtration prevents acute mortality and reduces chronic toxicity for early life stage coho salmon (Oncorhynchus kisutch) episodically exposed to urban stormwater runoff.

As the human population of western North America continues to expand, widespread patterns of urban growth pose increasingly existential threats to certain wild stocks of Pacific salmon and steelhead (Oncorhynchus sp.). Rainfall previously absorbed into the soils of forests and grasslands falls instead on pavement and other hardened surfaces. This creates stormwater runoff that carries toxic metals, oil, and many other contaminants into salmon-bearing habitats. These include freshwater streams where coho salmon (O. kisutch) spawn in gravel beds. Coho salmon embryos develop within a thick eggshell (chorion) for weeks to months before hatching as alevins and ultimately emerging from the gravel as fry. Untreated urban runoff is highly toxic to older coho salmon (freshwater-resident juveniles and adult spawners), but the vulnerability of the earliest life stages remains poorly understood. To address this uncertainty, we fertilized eggs and raised them under an episodic stormwater exposure regimen, using runoff collected from a high-traffic arterial roadway from 15 discrete storm events. We monitored survival and morphological development, as well as molecular markers for contaminant exposure and cardiovascular stress. We also evaluated the benefit of treating runoff with green infrastructure (bioretention filtration) on coho salmon health and survival. Untreated runoff caused subtle sublethal toxicity in pre-hatch embryos with no mortality, followed by high rates of mortality from exposure at hatch. Bioretention filtration removed most measured contaminants (bacteria, dissolved metals, and polycyclic aromatic hydrocarbons), and the treated effluent was considerably less toxic - notably preventing mortality at the alevin stage. Our findings indicate that untreated urban runoff poses an important threat to early life stage coho salmon, in terms of both acute and delayed-in-time mortality. Moreover, while inexpensive management strategies involving bioinfiltration are promising, future green infrastructure effectiveness research should emphasize sublethal metrics for contaminant exposure and adverse health outcomes in salmonids.

How to simulate future scenarios of urban stormwater management? A novel framework coupling climate change, urbanization, and green stormwater infrastructure development.

Climate change, urbanization, and green stormwater infrastructure (GSI) planning policies lead to uncertainties in future urban sustainability. Coupling multiple influencing factors such as climate change, urbanization, and GSI development, this study proposes a novel framework for simulating future scenarios of urban stormwater. Subsequently, the changes in annual surface runoff and runoff pollutants in Shanghai's new and old urban areas were compared and analyzed based on 35 typical future and seven baseline scenarios. The following results were obtained: 1) The runoff control rate of the new urban area was significantly higher than that of the old urban area before GSI construction. After GSI construction, both areas could control stormwater runoff and pollutants, while the decline in efficiency in GSI facilities enormously impacted the old area. 2) Surface runoff in the new urban area was mainly affected by urbanization, while climate change was a major factor in the old urban area; runoff pollutants in new and old urban areas were mainly affected by urbanization, and the change in pollutants in new areas was more pronounced. 3) GSI facilities were unlikely to guarantee the quantity and quality of water resources, especially in scenarios where the efficiency of GSI facilities decreases. In old urban areas, the more extreme climate change and urbanization were, the more significant the effect of improving stormwater management facilities. Our findings showed that future studies on stormwater management should specifically consider the different characteristics of new and old urban regions, pay attention to the maintenance and management of GSI facilities, and build adaptive strategies to cope with climate change, urbanization, and GSI facility destruction.

Supply-Demand Evaluation of Green Stormwater Infrastructure (GSI) Based on the Model of Coupling Coordination.

The rational spatial allocation of Green Stormwater Infrastructure (GSI), which is an alternative land development approach for managing stormwater close to the source, exerts a crucial effect on coordinating urban development and hydrological sustainability. The balance between the supply and demand of urban facilities has been an influential standard for determining the rationality of this allocation. However, at this stage, research on evaluating planning from the perspective of supply-demand in GSI is still limited. This study proposed an evaluation method for assessing supply-demand levels in GSIs in Guangzhou, China, using the coupling coordination model consisting of Coupling Degree (CD) and Coupling Coordination Degree (CCD). Furthermore, the spatial distributions of supply-demand balance and resource mismatch were identified. The results indicated that the supply and demand levels of GSI exhibited significant spatial differences in distribution, with most streets being in short supply. The GSI exhibited a high CD value of 0.575 and a poor CCD value of 0.328, implying a significant imbalance in facility allocation. A lot of newly planned facilities failed to effectively cover the streets in need of improvement, so it became essential to adjust the planning scheme. The findings of this study can facilitate the decision-makers in assessing the supply-demand levels in GSI and provide a reference of facility allocation for the sustainable construction of Sponge City.

Assessing the influence of urban greenness and green stormwater infrastructure on hydrology from satellite remote sensing.

Green stormwater infrastructure (GSI), which includes features like rain gardens, constructed wetlands, or urban tree canopy, is now widely recognized as a means to reduce urban runoff impacts and meet municipal water quality permit requirements. Many co-benefits of GSI are related to increased vegetative cover, which can be measured with satellite imagery via spectral indices such as the Normalized Difference Vegetation Index (NDVI). In urban landscapes, there remain critical gaps in understanding how urban greenness and GSI influence hydrology. Here, we quantify these relationships to assess the feasibility of tracking the effectiveness of urban greening for improving downstream hydrologic conditions. We combined hydrologic data from the United States Geological Survey (USGS) gauges with an NDVI time series (1985-2019) derived from Landsat satellite imagery, and synthesis of GSI implementation data from a set of 372 urbanized watersheds across the United States. We used a multivariate panel modeling approach to account for spatial and time varying factors (rainfall, temperature, urban cover expansion) in an effort to isolate the relationships of interest. After accounting for expansion of urban boundaries, only 32 watersheds (9%) showed significant greenness trends, a majority of which were reductions. Urban greenness had significant influences on downstream flow responses, so that on average, a 10% greenness increase showed a corresponding reduction of total flow (-3.8%), flow variance (-7.7%), peak flows (-4.7%), high flows (-7.6%), flashiness (-2.2%), and high flow frequency (-1.5%); and a corresponding increase in baseflow (4.3%). For a subset of these watersheds for which GSI data were available (n = 48), the level of GSI implementation showed a significant, but weak influence on urban greenness with a 20% increase in BMP density corresponding to a greenness increase of 0.9%. The study results may support valuation and verification of GSI co-benefits in urbanized landscapes at the watershed scale.

Greening Up For Mosquitoes: A Comparison of Green Stormwater Infrastructure in a Semiarid Region.

Green stormwater infrastructure provides environmental, economic, and health benefits as a strategy for building resilience against climate change impacts. However, it may inadvertently increase vulnerability due to improper design and construction or lack of maintenance. We engaged city stakeholders and a diverse student group to investigate possible maladaptation. After rain events, student interns collected data at green stormwater infrastructure, identified in partnership with city stakeholders, for both water retention and mosquito larvae, if present. During the sampling period in 2018, 24 rain events occurred, with 28 sites visited 212 times including visits to basins (63%), curb cuts (34%), and a bioswale (2%). The largest basin consistently retained water (mean: 3.3 days, SD: 2.3 days) and was a positive site for Culex quinquefasciatus, a West Nile virus vector. We found that while basins can become mosquito breeding habitat, there was no evidence that curb cuts were collecting and retaining water long enough. As cities turn to green stormwater infrastructure to address climate change impacts of increasing drought, flooding, and extreme heat, these findings can help in the selection of appropriate infrastructure design typologies.

"Green Enough Ain't Good Enough:" Public Perceptions and Emotions Related to Green Infrastructure in Environmental Justice Communities.

The concept of biophilic urban planning has inspired neighborhood greening projects in many older urban communities in the USA and beyond. The strengths (e.g., environmental management, biodiversity, heat island mitigation) and challenges (e.g., greenwashing, green gentrification) of such projects are well-documented. Additional research on the relationship between these projects and various social factors (e.g., public perceptions, feelings, and mental health and well-being) is necessary to better understand how people adapt to said projects while struggling to navigate other more pressing socioeconomic issues, especially in communities facing environmental injustice and health inequity. In this article, we focus on one aspect of biophilic urban planning-green stormwater infrastructure (GSI) (e.g., rain gardens, bio-swales, pervious pavements, and wildflower meadows)-in Waterfront South, a post-industrial neighborhood in Camden, NJ, USA, where residents have faced environmental injustices for decades. Our qualitative analysis of in-depth semi-structured interviews of sixteen residents offered a thorough insight into their perceptions and emotions regarding different types of urban GSI projects. Residents acknowledge the many benefits that GSI offers to combat the neighborhood's social and environmental injustices, but they are cautious about the possibility of some projects prompting new issues and concerns within the community. Our findings reveal potential implications in GSI planning, research, and practice in this neighborhood and similar urban places elsewhere that have yet to undergo gentrification.

Influence of soil characteristics and metal(loid)s on antibiotic resistance genes in green stormwater infrastructure in Southern California.

The synergetic effects of metal(loid)s and soil characteristics on bacterial antibiotic resistance genes (ARGs) in green stormwater infrastructure (GSI) has been relatively understudied. Surface soil samples from six GSIs in Southern California over three time periods were assessed for selected ARGs, class 1 integron-integrase genes (intI1), 16S rRNA genes, and bioavailable and total concentrations of nine metal(loid)s, to investigate the relationships among ARGs, soil characteristics, and co-occurring metal(loid)s. Significant correlations existed among relative gene abundances (sul1, sul2, tetW, and intI1), total metal(loid)s (arsenic, copper, lead, vanadium, and zinc), and bioavailable metal(loid) (arsenic) (r = 0.29-0.61, padj < 0.05). Additionally, soil texture, organic matter, and nutrients within GSI appeared to be significantly correlated with relative gene abundances of sul1, sul2, and tetW (r = -0.57 to 0.59, padj < 0.05). Multiple regression models significantly improved the estimation of ARGs in GSI when considering multiple effects of soil characteristics and metal(loid)s (r = 0.74, padj < 0.001) compared to correlation results. Total arsenic was a significant (positive) correlate in all the regression models of relative gene abundances. This work provides new insights into co-dependencies between GSI ARGs and co-occurring metal(loid)s, indicating the need for risk assessment of metal(loid)-influenced ARG proliferation.

Balancing Hydraulic Control and Phosphorus Removal in Bioretention Media Amended with Drinking Water Treatment Residuals.

Green stormwater infrastructure like bioretention can reduce stormwater runoff volumes and trap sediments and pollutants. However, bioretention soil media can be both a sink and source of phosphorus (P). We investigated the potential tradeoff between hydraulic conductivity and P sorption capacity in drinking water treatment residuals (DWTRs), with implications for bioretention media design. Batch isotherm and flow-through column experiments were used to quantify the maximum P sorption capacity (Smax) and rate of P sorption for three DWTR sources. Smax values varied greatly among DWTR sources and methodologies, which has implications for regulatory standards. We also conducted a large column experiment to determine the hydraulic and P removal effects of amending bioretention media with solid and mixed layers of DWTRs. When applied to bioretention media, the impact of DWTRs on hydraulic conductivity and P removal depended on layering strategy. Although DWTR addition in solid and mixed layer designs improved P removal, the solid layer restricted water flow and exhibited incomplete P removal, while the mixed layer had no effect on flow and removed ~100% of P inputs. We recommend that DWTRs be mixed with sand in bioretention media to simultaneously achieve stormwater drainage and P reduction goals in green stormwater infrastructure.