Glandular odontogenic cyst: a case report in an asymptomatic patient.

OBJECTIVE: Glandular odontogenic cyst is an extremely rare lesion of the jaw. Its aggressive behavior and high recurrence rate makes it important while excising. Both clinical and radiographic features were nonspecific due to the similarities with other lesions therefore it can be misdiagnosed very easily. This article presents another case of Glandular odontogenic cyst in 17 year of male patient who was unaware of the lesion which was detected on routine examination.

First case of disseminated cryptococcosis in a Gorilla gorilla.

In humans, Cryptococcus mainly infects individuals with HIV infection or other types of immunosuppression. Here, we report the first case of disseminated cryptococcosis in a simian immunodeficiency virus-negative 27-year-old female Gorilla gorilla presenting with lethargy, progressive weight loss and productive cough. The diagnosis was confirmed by positive lung biopsy culture, serum cryptococcal antigen, and cerebral histopathology demonstrating encapsulated yeasts. Molecular characterisation of lung culture isolate yielded Cryptococcus neoformans var. grubii. An immune-deficiency could not be demonstrated.

Mucoepidermoid carcinoma of the lower lip: A case report and review of literature.

Mucoepidermoid carcinoma (MEC) is a rare salivary gland malignancy characterized by diverse cellular components. This case report presents a unique instance of low-grade MEC in a 28-year-old female, emphasizing the importance of accurate diagnosis and tailored management. The patient exhibited a painless, enlarging lower lip swelling over a year, with imaging suggestive of a fibrolipomatous lesion. Surgical excision was successfully performed, with subsequent histopathological analysis displaying mucinous cystic spaces, varied epithelial cell types and other characteristic features consistent with MEC. The case findings aligned with hallmark MEC features are seen in established literature, highlighting the significance of precise diagnosis and grading for appropriate management. This report contributes to the understanding of MEC's varied presentations and underscores the importance of thorough: histopathological examination, lower lip, mucoepidermoid carcinoma clinical evaluation, accurate histopathological analysis and interdisciplinary collaboration. The case highlights the value of considering MEC in younger patients, even with atypical presentations, and encourages ongoing exploration of diagnostic and therapeutic strategies to improve patient outcomes.

Agreement Between Mucicarmine-Stained Bronchial Brushing and Bronchial Biopsy in Subtyping Non-small Cell Lung Carcinoma.

Background Bronchial brushing and biopsy are used for the diagnosis of lung carcinoma as most of these tumors are unresectable. Recently, the subclassification of non-small cell lung carcinoma (NSCLC) into adenocarcinoma (ADC) and squamous cell carcinoma (SCC) has become mandatory due to the emergence of targeted therapies. Due to the inherent limitations of small samples, subcategorization of a tumor is not always possible. Immunohistochemical and mucin stains are being used for this purpose, especially for tumors with poorly differentiated features. In our study, we utilized mucicarmine mucin stain to refine the differentiation of SCC and ADC on bronchial brushing and determine its agreement with bronchial biopsy. This study aimed to measure the degree of agreement between mucicarmine-stained bronchial brushing and bronchial biopsy for subtyping NSCLC into SCC and ADC. Methodology This descriptive, cross-sectional study was conducted in the pathology department of Allama Iqbal Medical College. Samples were collected by the pulmonology department of Jinnah Hospital Lahore. The study was conducted for 10 months from June 2020 to April 2021. A total of 60 cases diagnosed as NSCLC, aged between 35 and 80 years, were included in this study. After cytohistological evaluation of bronchial brushing and bronchial biopsy specimens, the agreement was deduced using kappa statistics. Results The strength of agreement between mucicarmine-stained bronchial brushing and bronchial biopsy for subtyping NSCLC into SCC and ADC was found to be substantial. Conclusions As significant agreement exists between the two modalities, mucicarmine-stained bronchial brushing can be used for a reliable and rapid categorization of NSCLC.

Evaluation of Efficacy of Microwave Staining over Conventional Staining in Replicating Tissue Architecture: A Prospective Study.

INTRODUCTION: The use of microwave in the field of diagnostic pathology has gained a huge response in recent times. Use of domestic microwave ovens in the same is being widely studied. Unveiling the use of the microwave in improving the staining quality of tissue sections in the field of pathology can aid in precise diagnosis of complex conditions. AIM: The main aim of this study was to study the efficiency of microwave staining to reproduce the tissue architecture compared to that of conventional staining techniques. MATERIALS AND METHODS: Thirty different tissue blocks (including 10 mucocele tissue blocks) were used to prepare 30 pairs of slides for three different stains, namely hematoxylin and eosin, Van Gieson's, 0.1% toluidine blue and periodic acid-Schiff and 10 pairs of slides for mucicarmine stain. From each pair of slides, one slide was stained routinely, and the other was stained inside a microwave. Two pathologists evaluated the stained slides, and the results so obtained were analyzed statistically. RESULTS: Microwave staining considerably cut down the staining time from hours to seconds. Microwave staining showed no loss of cellular and nuclear details, uniform staining characteristics, and was of excellent quality. CONCLUSION: The microwave stained slide showed no significant difference in terms of cell outline clarity, cytoplasmic staining, nuclear outline, nuclear staining, nuclear chromatin, and staining intensity compared to that of routine staining method, and a significant difference was observed in the total staining time consumed by all the stains that were used.

Documentation of postmortem changes in salivary gland architecture and staining characteristics.

CONTEXT: Estimation of time passed since death continues to be a major problem for the forensic pathologist and its determination plays an important and vital role in medico-legal cases. The histological studies on various tissues after death have been mostly confined to single organ or tissue by individual workers at different atmospheric conditions. AIMS: The aim of this study is to determine the best rehydrating solution for dehydrated tissues in postmortem examination. SETTINGS AND DESIGN: This study was specific to salivary gland tissues and certain pattern of changes were determined during postmortem time intervals using hematoxylin and eosin stain and special stains like mucicarmine and alcian blue. MATERIALS AND METHODS: The study was divided into two groups. (1) Group A: Normal tissue samples (twenty normal salivary gland tissue samples left without fixation for varying periods of time). (2) Group B: Control group (twenty normal salivary gland tissue samples immediately fixed in formalin). The three different rehydrating agents used in this study were glycerol, normal saline and modified Ruffer solution. STATISTICAL ANALYSIS USED: Not required. RESULTS: Modified Ruffer solution is the best when compared to glycerol and normal saline for rehydration of dehydrated tissues. CONCLUSIONS: Thus in our study we conclude that the tissue which had been dehydrated at the crime scene for a fairly long period showed better rehydration with modified Ruffer solution and yield good cellular and nuclear details.

Invasive lobular carcinoma of the breast with extracellular mucin- a new rare variant.

Historically, extracellular mucin secretion is by default a feature of ductal carcinoma. Lobular carcinoma is known to be associated with only intracytoplasmic mucin production and signet ring cells. As ductal carcinoma and lobular carcinoma have different therapeutic and prognostic implications, it is important to classify them into respective groups. Here, we present a case of invasive lobular carcinoma with abundant extracellular and intracellular mucin in a 38-year-old female patient. Invasive lobular carcinoma with extracellular mucin is an extremely rare and new variant. Only 3 cases are reported in the literature so far.

Rhinosporidiosis in the parotid duct: a rare case report.

Endemic to South India and Sri Lanka, Rhinosporidiosis is a chronic granulomatous infection caused by an agent of uncertain taxonomy: Rhinosporidium seeberi. Although it commonly manifests as a proliferative nasal lesion, many cases of Rhinosporidiosis have been reported where it has appeared as an extranasal lesion. The reported extranasal sites include the eye, ear, trachea, and parotid duct. However, the involvement of the parotid duct is quite rare, even among extranasal sites. The case presented is an adult female from the non-endemic zone of East India with a proliferative mass in the parotid duct. Although Rhinosporidiosis was not taken into consideration in the clinical differential diagnosis, eventual histopathological diagnosis confirmed Rhinosporidiosis. As this appears to be the second case of Rhinosporidiosis in the parotid duct in East India in 4 years, we encourage clinicians to be flexible in the differential diagnosis of proliferative growth in the parotid duct, even in those from non-endemic areas.

Clear cell variant of intraosseous mucoepidermoid carcinoma: Report of a rare entity.

Intraosseous mucoepidermoid carcinoma of jaw bones is a rare lesion. Abundance of clear cells in an intraosseous mucoepidermoid carcinoma may complicate its histopathologic diagnosis. It becomes extremely important to distinguish this lesion from other clear cell lesions of jaw region. Here, we report a case of clear cell variant of intraosseous mucoepidermoid carcinoma in the mandible.

Histopathology, serology and cultures in the diagnosis of cryptococcosis / Histopatologia, sorologia e cultivo no diagnóstico da criptococose

Cryptococcosis is one of the most common opportunistic fungal infections in patients with acquired immunodeficiency syndrome (AIDS). We report 13 cases of cryptococcal infection based on histopathology, serology and cultures. Epidemiological analysis, histochemical techniques of hematoxilin and eosin (HE) and Grocot's silver (GMS), as well special histochemical techniques such as Mayer's mucicarmine (MM) and Fontana-Masson (FM), cryptococcal antigen test (CrAg) and isolation on fungal media: Sabouraud's (SAB), brain-heart infusion agar (BHI) and canavanine-glycine-bromothymol blue (CGB) agar were analyzed. Unsatisfactory staining results by MM stain associated to negative titers by CrAg test, which FM stain confirmed that capsule-deficient Cryptococcus infections were observed in four cases. Eight isolated cases were identified as follows: six cases were infection with Cryptococcus neoformans and two cases were Cryptococcus gattii.

A criptococose é a mais comum infecção fúngica oportunística observada em pacientes com síndrome da imunodeficiência adquirida (AIDS). Relatamos 13 casos da infecção baseados no diagnóstico histopatológico, sorológico e cultivo. Foram analisadas: a epidemiologia, as técnicas histoquímicas básicas de hematoxilina-eosina (HE) e coloração pela prata (GMS), bem como as técnicas histoquímicas especiais de mucicarmim de Mayer (MM) e Fontana-Masson (FM), o teste do antígeno criptocóccico (CrAg) e o isolamento em cultivos em ágar-Sabouraud (SAB), ágar infusão de cérebro-coração (BHI) e meio com canavanina azul de bromotimol (CGB). Em quatro casos, resultados tintoriais insatisfatórios pela coloração de MM associados a títulos negativos pelo teste do CrAg, a coloração de FM confirmou a infecção pelo Cryptococcus deficiente de cápsula. Oito isolados foram identificados: seis casos apresentaram a infecção por Cryptococcus neoformans e dois casos apresentaram a infecção por Cryptococcus gattii.

Técnicas histopatológicas no diagnóstico de criptococose por Cryptococcus deficiente de cápsula: relato de caso / Histopathological techniques for diagnosing cryptococcosis due to capsule-deficient Cryptococcus: case report

Caso de criptococose por Cryptococcus deficiente de cápsula, no qual cultivo do espécime clínico e pesquisa do antígeno capsular no líquor e soro foram negativos. As técnicas histopatológicas foram: Hematoxilina-eosina, Grocott, Mucicarmim de Mayer e Fontana-Masson. Confirmou-se o diagnóstico do Cryptococcus deficiente de cápsula pela técnica de Fontana-Masson e pela imunofluorescência direta. É discutida a potencialidade das técnicas histoquímicas.

A case of cryptococcosis due to capsule-deficient Cryptococcus is presented, in which culturing of the clinical specimen and tests for capsular antigen in cerebrospinal fluid and serum were negative. The histopathological techniques evaluated were hematoxylin-eosin, Grocott methenamine silver, Mayer’s mucicarmine and Fontana-Masson. The diagnosis of cryptococcosis due to capsule-deficient Cryptococcus was confirmed by means of the Fontana-Masson technique and by direct immunofluorescence. The potential of the histochemical techniques is discussed.