Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.

Alternative 3' untranslated regions (3' UTRs) are widespread, but their functional roles are largely unknown. We investigated the function of the long BIRC3 3' UTR, which is upregulated in leukemia. The 3' UTR does not regulate BIRC3 protein localization or abundance but is required for CXCR4-mediated B cell migration. We established an experimental pipeline to study the mechanism of regulation and used mass spectrometry to identify BIRC3 protein interactors. In addition to 3'-UTR-independent interactors involved in known BIRC3 functions, we detected interactors that bind only to BIRC3 protein encoded from the mRNA with the long 3' UTR. They regulate several functions, including CXCR4 trafficking. We further identified RNA-binding proteins differentially bound to the alternative 3' UTRs and found that cooperative binding of Staufen and HuR mediates 3'-UTR-dependent complex formation. We show that the long 3' UTR is required for the formation of specific protein complexes that enable additional functions of BIRC3 protein beyond its 3'-UTR-independent functions.

Core microbiota drive multi-functionality of the soil microbiome in the Cinnamomum camphora coppice planting.

BACKGROUND: Cinnamomum camphora (L.) Presl (C. camphora) is an evergreen broad-leaved tree cultivated in subtropical China. The use of C. camphora as clonal cuttings for coppice management has become popular recently. However, little is known about the relationship between soil core microbiota and ecosystem multi-functionality under tree planting. Particularly, the effects of soil core microbiota on maintaining ecosystem multi-functionality under C. camphora coppice planting remained unclear. MATERIALS AND METHODS: In this study, we collected soil samples from three points (i.e., the abandoned land, the root zone, and the transition zone) in the C. camphora coppice planting to investigate whether core microbiota influences ecosystem multi-functions. RESULTS: The result showed a significant difference in soil core microbiota community between the abandoned land (AL), root zone (RZ), and transition zone (TZ), and soil ecosystem multi-functionality of core microbiota in RZ had increased significantly (by 230.8%) compared to the AL. Soil core microbiota played a more significant influence on ecosystem multi-functionality than the non-core microbiota. Moreover, the co-occurrence network demonstrated that the soil ecosystem network consisted of five major ecological clusters. Soil core microbiota within cluster 1 were significantly higher than in cluster 4, and there is also a higher Copiotrophs/Oligotrophs ratio in cluster 1. Our results corroborated that soil core microbiota is crucial for maintaining ecosystem multi-functionality. Especially, the core taxa within the clusters of networks under tree planting, with the same ecological preferences, had a significant contribution to ecosystem multi-functionality. CONCLUSION: Overall, our results provide further insight into the linkage between core taxa and ecosystem multi-functionality. This enables us to predict how ecosystem functions respond to the environmental changes in areas under the C. camphora coppice planting. Thus, conserving the soil microbiota, especially the core taxa, is essential to maintaining the multiple ecosystem functions under the C. camphora coppice planting.

Nitrate Radical Induced "Two in One" Interface Engineering toward High Reversibility of Zn Metal Anode.

Advanced interfacial engineering performs a forceful modulation effect on Zn2+ plating/stripping with simultaneous inhibition of hydrogen evolution reaction, chemical corrosion, and dendrite growth, which is responsible for high reversibility of Zn anode. Herein, a "two in one" interface engineering is developed to improve the reversibility of Zn anode, in which multi-functional Zn5 (NO3 )2 (OH)8 ·2H2 O layer and preferential Zn (002) texture are constructed simultaneously. Due to nucleophilicity to Zn2+ arising from electronegativity, the layer can accelerate the desolvation process of [Zn (H2 O)6 ]2+ and transfer kinetics of Zn2+ ions, leading to uniform nucleation and effective inhibition of water-induced side reactions. Meanwhile, the latter is beneficial to guiding   Zn (002)-preferred orientation deposition with compact structure. Consequently, the Zn electrodes with such complementary interface modulation exhibit prominent reversibility. With an area capacity of 1 mAh cm-2 at 1 mA cm-2 , the symmetric cell operates steadily for 4000 h. Highly reversible Zn anode is maintained even at 50 mA cm-2 . For full cells coupled with MnO2 cathode, impressive rate capability and cycling stability with a high capacity beyond 100 mAh g-1 at 1 A g-1 after 2000 cycles are achieved. The results provide new insights into Zn anodes with high reversibility for next-generation aqueous zinc ion batteries.

3D Printed Graphene-Based Metamaterials: Guesting Multi-Functionality in One Gain.

Advanced functional materials with fascinating properties and extended structural design have greatly broadened their applications. Metamaterials, exhibiting unprecedented physical properties (mechanical, electromagnetic, acoustic, etc.), are considered frontiers of physics, material science, and engineering. With the emerging 3D printing technology, the manufacturing of metamaterials becomes much more convenient. Graphene, due to its superior properties such as large surface area, superior electrical/thermal conductivity, and outstanding mechanical properties, shows promising applications to add multi-functionality into existing metamaterials for various applications. In this review, the aim is to outline the latest developments and applications of 3D printed graphene-based metamaterials. The structure design of different types of metamaterials and the fabrication strategies for 3D printed graphene-based materials are first reviewed. Then the representative explorations of 3D printed graphene-based metamaterials and multi-functionality that can be introduced with such a combination are further discussed. Subsequently, challenges and opportunities are provided, seeking to point out future directions of 3D printed graphene-based metamaterials.

Why are biodiversity-ecosystem functioning relationships so elusive? Trophic interactions may amplify ecosystem function variability.

The relationship between biodiversity and ecosystem functions (BEFs) has attracted great interest. Studies on BEF have so far focused on the average trend of ecosystem function as species diversity increases. A tantalizing but rarely addressed question is why large variations in ecosystem functions are often observed across systems with similar species diversity, likely obscuring observed BEFs. Here we use a multi-trophic food web model in combination with empirical data to examine the relationships between species richness and the variation in ecosystem functions (VEFs) including biomass, metabolism, decomposition, and primary and secondary production. We then probe the mechanisms underlying these relationships, focusing on the role of trophic interactions. While our results reinforce the previously documented positive BEF relationships, we found that ecosystem functions exhibit significant variation within each level of species richness and the magnitude of this variation displays a hump-shaped relationship with species richness. Our analyses demonstrate that VEFs is reduced when consumer diversity increases through elevated nonlinearity in trophic interactions, and/or when the diversity of basal species such as producers and decomposers decreases. This explanation is supported by a 34-year empirical food web time series from the Gulf of Riga ecosystem. Our work suggests that biodiversity loss may not only result in ecosystem function decline, but also reduce the predictability of functions by generating greater function variability among ecosystems. It thus helps to reconcile the debate on the generality of positive BEF relationships and to disentangle the drivers of ecosystem stability. The role of trophic interactions and the variation in their strengths mediated by functional responses in shaping ecosystem function variation warrants further investigations and better incorporation into biodiversity-ecosystem functioning research.

A large share of climate impacts of beef and dairy can be attributed to ecosystem services other than food production.

Domesticated ruminants supply nutrient-dense foods but at a large environmental cost. However, many ruminant production systems are multi-functional, providing ecosystem services (ES) other than direct provision of food. When quantifying the climate impact of ruminant products using life cycle assessment (LCA), provisioning ES (i.e. beef and milk) are generally considered the only valuable outputs and other ES provided are ignored, which risks overlooking positive contributions associated with ruminant production. Non-provisioning ES can be included in LCA by economic allocation, using compensatory payments (through agri-environmental schemes) as a proxy for the economic value of ES. For example, farmers can receive payments for maintenance of pastures, which supports e.g. pollination. However, the association between different payment schemes, the ES provided, and livestock production is not always straightforward and it can be difficult to determine which payment schemes to include in the allocation. This study examined how accounting for ES in quantification of climate impact for beef and milk production on Swedish farms was affected by different ways of coupling ES to livestock production through payment schemes. Quantification was done using LCA, attributing the climate impact to beef, milk, and other ES by economic allocation. This resulted in <1-48% and 11-31% of climate impacts being allocated to other ES, instead of beef and milk, respectively, affecting suckler farms most. The results were influenced by which payment schemes, representing different ES, that were included; when only payments directly related to livestock rearing were included, the difference in the climate impact was still large between farm types, while the difference decreased considerably when all environmental schemes were included. While emissions do not disappear, ES-corrected climate impact can potentially be useful as part of consumer communication or in decision-making, reducing the risk of overlooking ES provided by ruminant production in a simpler way than using separate indicators.

Internal causality in agri-food Life Cycle Assessments: Solving allocation problems based on feed energy utilization in egg production.

Physical allocation in Life Cycle Assessment (LCA) should, ideally, be based on underlying causal relationships. While both cause-oriented and effect-oriented causality referred to in LCA literature are forms of external causality, internal causality addresses the actual flow of materials and inputs in a system - in other words, the real behaviour of the system under study. While a number or examples of allocation based on physical causality have been used in poultry LCAs, none of these represent the internal causality (the actual biological processes) in egg production. The current study remedies that gap by proposing such a method. Agri-food LCAs, in particular LCAs of livestock production, were used to identify existing physical allocation approaches consistent with internal causality. The most commonly used approach was found to be based on the allocation of feed energy to support the various physiological functions of the livestock species. A feed energy - Metabolizable Energy (ME) - utilization model for allocation in egg production LCAs is hence similarly proposed. Using the inventory of a previous LCA study of egg production in Canada, allocation ratios for eggs and spent hens were developed. Feed utilization models specific to each unit process were identified. The overall differences between ME utilization (∼95% eggs, 5% spent hens) and gross chemical energy content (92% eggs, 8% spent hens) for allocation were relatively small. Scenario analysis, however, showed that the allocation ratios can be considerably different if the causal relationship is interpreted differently. Differences over ∼20% was seen in a scenario which did not allocate between the co-products of each unit process in the system, but rather to the products at the end of a biological causal chain straddling multiple unit processes. The proposed approach is consistent with the interpretation of LCA as a natural sciences framework, and with the ISO 14044 multi-functionality hierarchy, because it reflects actual biological causality in egg production systems. The study results also underscore that practitioners should not only clearly justify their choice of allocation strategy, but also describe its application in detail, since small differences in methods can result in divergent outcomes.

Agroforestry trade-offs between biomass provision and aboveground carbon sequestration in the alpine Eisenwurzen region, Austria.

Mountain agroecosystems deliver essential ecosystem services to society but are prone to climate change as well as socio-economic pressures, making multi-functional land systems increasingly central to sustainable mountain land use policy. Agroforestry, the combination of woody vegetation with crops and/or livestock, is expected to simultaneously increase provisioning and regulating ecosystem services, but knowledge gaps concerning trade-offs exist especially in temperate industrialized and alpine regions. Here, we quantify the aboveground carbon (C) dynamics of a hypothetical agroforestry implementation in the Austrian long-term socio-ecological research region Eisenwurzen from 2020 to 2050. We develop three land use scenarios to differentiate conventional agriculture from an immediate and a gradual agroforestry implementation, integrate data from three distinct models (Yield-SAFE, SECLAND, MIAMI), and advance the socio-ecological indicator framework Human Appropriation of Net Primary Production (HANPP) to assess trade-offs between biomass provision and carbon sequestration. Results indicate that agroforestry strongly decreases HANPP because of a reduction in biomass harvest by up to - 47% and a simultaneous increase in actual net primary production by up to 31%, with a large amount of carbon sequestered in perennial biomass by up to 3.4 t C ha-1 yr-1. This shows that a hypothetical transition to agroforestry in the Eisenwurzen relieves the agroecosystem from human-induced pressure but results in significant trade-offs between biomass provision and carbon sequestration. We thus conclude that while harvest losses inhibit large-scale implementation in intensively used agricultural regions, agroforestry constitutes a valuable addition to sustainable land use policy, in particular when affecting extensive pastures and meadows in alpine landscapes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10113-021-01794-y.

Managing flooding: from a problem to an opportunity.

The paper argues that the concept and practice of sustainability have proved too difficult to achieve within traditional water management, and there is a lack of political will to move towards truly sustainable water services. Instead, compromised concepts, including resistance, resilience, ecosystem services, natural capital and adaptation are defining approaches; each of which may contribute partially to sustainability. Pressures due to the changing climate, ecological degradation, human demands, urbanization and deteriorating assets are challenging sustainability and compelling changes to water management. Water is now seen less as a problem to be managed than as an opportunity, as wherever situated, water brings many opportunities to contribute to anthropogenic needs. New ideas are helping to frame the way in which water management is being approached: (i) waste is no longer waste, but a potential resource within a circular economy; (ii) the interconnectedness of infrastructure systems and services and circularity of the water cycle mean there is a need to integrate approaches; (iii) nature-based systems should be preferenced for water infrastructure. These issues and ideas are considered here, together with examples of schemes showing that managing flooding can lead to wider benefits, and potential longer-term sustainability. This article is part of the theme issue 'Urban flood resilience'.

Protein multi-functionality: introduction.

The five articles in this multi-author review in CMLS provide examples of multi-functionality of proteins belonging to several families. Distinct structural features of proteins suggesting multi-functionality are emphasized: intrinsically disordered elements that can "mold" themselves to fit various binding partners, as well as short linear motifs within larger proteins that perform particular functions. Although only a few protein families are discussed in detail, the conclusions apply to numerous, if not all, proteins. Multi-functionality of virtually every protein implies that the manipulation of its expression levels by over-expression, knockdown, or knockout affects every one of its functions, known and unknown, so that the results of these experiments must be interpreted with this complexity in mind. Particular functions in a multi-functional protein are often fulfilled by identifiable smaller elements that can be expressed separately. Identification of mono-functional elements of a multi-functional protein paves the way to the construction of novel precisely targeted molecular tools for selective manipulation of cellular signaling that can be used for mechanistic studies in cell biology, as well as for therapeutic purposes.

Versatile Pt NCs-based chemotherapeutic agents significantly induce the apoptosis of cisplatin-resistant non-small cell lung cancer.

Recently, the incidence of lung cancer is generally rising along with air pollution and smoking, and non-small cell lung cancer (NSCLC) accounts for nearly 85% among all lung cancer diagnoses. With the development of chemotherapy, the drug resistance rate of common platinum-based chemotherapeutic drugs (like cisplatin) is gradually increased, which seriously affects the chemotherapy efficiency and survival rate of patients. In this study, polyethylenimine caged platinum nanoclusters (PEI-caged Pt NCs) were proposed as a new chemotherapeutic agent to apply in the treatment of NSCLC, choosing the classical cisplatin-resistant A549/DDP cells and normal A549 cells as targets. It was found that our Pt NCs-based chemotherapeutic drugs showed its preferable therapeutic effect in cisplatin-resistant NSCLC through the results of confocal microscopic images, cell counting kit-8 test, cell apoptosis assay and western blot. Most importantly, in the cisplatin-resistance A549/DDP cells, this kind of agents could enter the nucleus obviously, and emerged a superior inhibitory and apoptotic effects than A549 via activating p53 protein and the related signaling pathways. Comparing with the traditional chemotherapy drugs, these Pt NCs-based chemotherapeutic agents exhibit great potential and advantages in the treatment and diagnosis of NSCLC regardless of the therapeutic effect or toxic side effects, especially the drug resistance.

Natural Fibre-Reinforced Polymer Composites (NFRP) Fabricated from Lignocellulosic Fibres for Future Sustainable Architectural Applications, Case Studies: Segmented-Shell Construction, Acoustic Panels, and Furniture.

Due to the high amounts of waste generated from the building industry field, it has become essential to search for renewable building materials to be applied in wider and more innovative methods in architecture. One of the materials with the highest potential in this area is natural fibre-reinforced polymers (NFRP), which are also called biocomposites, and are filled or reinforced with annually renewable lignocellulosic fibres. This would permit variable closed material cycles' scenarios and should decrease the amounts of waste generated in the building industry. Throughout this paper, this discussion will be illustrated through a number of developments and 1:1 mockups fabricated from newly developed lignocellulosic-based biocomposites from both bio-based and non-bio-based thermoplastic and thermoset polymers. Recyclability, closed materials cycles, and design variations with diverse digital fabrication technologies will be discussed in each case. The mock-ups' concepts, materials' compositions, and fabrication methods are illustrated. In the first case study, a structural segmented shell construction is developed and constructed. In the second case study, acoustic panels were developed. The final case studies are two types of furniture, where each is developed from a different lignocellulosic-based biocomposite. All of the presented case studies show diverse architectural design possibilities, structural abilities, and physical building characteristics.

Evolutionary Perspectives of Genotype-Phenotype Factors in Leishmania Metabolism.

The sandfly midgut and the human macrophage phagolysosome provide antagonistic metabolic niches for the endoparasite Leishmania to survive and populate. Although these environments fluctuate across developmental stages, the relative changes in both these environments across parasite generations might remain gradual. Such environmental restrictions might endow parasite metabolism with a choice of specific genotypic and phenotypic factors that can constrain enzyme evolution for successful adaptation to the host. With respect to the available cellular information for Leishmania species, for the first time, we measure the relative contribution of eight inter-correlated predictors related to codon usage, GC content, gene expression, gene length, multi-functionality, and flux-coupling potential of an enzyme on the evolutionary rates of singleton metabolic genes and further compare their effects across three Leishmania species. Our analysis reveals that codon adaptation, multi-functionality, and flux-coupling potential of an enzyme are independent contributors of enzyme evolutionary rates, which can together explain a large variation in enzyme evolutionary rates across species. We also hypothesize that a species-specific occurrence of duplicated genes in novel subcellular locations can create new flux routes through certain singleton flux-coupled enzymes, thereby constraining their evolution. A cross-species comparison revealed both common and species-specific genes whose evolutionary divergence was constrained by multiple independent factors. Out of these, previously known pharmacological targets and virulence factors in Leishmania were identified, suggesting their evolutionary reasons for being important survival factors to the parasite. All these results provide a fundamental understanding of the factors underlying adaptive strategies of the parasite, which can be further targeted.

A spectrum of modularity in multi-functional gene circuits.

A major challenge in systems biology is to understand the relationship between a circuit's structure and its function, but how is this relationship affected if the circuit must perform multiple distinct functions within the same organism? In particular, to what extent do multi-functional circuits contain modules which reflect the different functions? Here, we computationally survey a range of bi-functional circuits which show no simple structural modularity: They can switch between two qualitatively distinct functions, while both functions depend on all genes of the circuit. Our analysis reveals two distinct classes: hybrid circuits which overlay two simpler mono-functional sub-circuits within their circuitry, and emergent circuits, which do not. In this second class, the bi-functionality emerges from more complex designs which are not fully decomposable into distinct modules and are consequently less intuitive to predict or understand. These non-intuitive emergent circuits are just as robust as their hybrid counterparts, and we therefore suggest that the common bias toward studying modular systems may hinder our understanding of real biological circuits.

Measuring the sustainability of a natural system by using multi-criteria distance function methods: Some critical issues.

There is an important body of literature using multi-criteria distance function methods for the aggregation of a battery of sustainability indicators in order to obtain a composite index. This index is considered to be a proxy of the sustainability goodness of a natural system. Although this approach has been profusely used in the literature, it is not exempt from difficulties and potential pitfalls. Thus, in this paper, a significant number of critical issues have been identified showing different procedures capable of avoiding, or at least of mitigating, the inherent potential pitfalls associated with each one. The recommendations made in the paper could increase the theoretical soundness of the multi-criteria distance function methods when this type of approach is applied in the sustainability field, thus increasing the accuracy and realism of the sustainability measurements obtained.

A Multi-Functional Microelectrode Array Featuring 59760 Electrodes, 2048 Electrophysiology Channels, Stimulation, Impedance Measurement and Neurotransmitter Detection Channels.

Biological cells are characterized by highly complex phenomena and processes that are, to a great extent, interdependent. To gain detailed insights, devices designed to study cellular phenomena need to enable tracking and manipulation of multiple cell parameters in parallel; they have to provide high signal quality and high spatiotemporal resolution. To this end, we have developed a CMOS-based microelectrode array system that integrates six measurement and stimulation functions, the largest number to date. Moreover, the system features the largest active electrode array area to date (4.48×2.43 mm2) to accommodate 59,760 electrodes, while its power consumption, noise characteristics, and spatial resolution (13.5 µm electrode pitch) are comparable to the best state-of-the-art devices. The system includes: 2,048 action-potential (AP, bandwidth: 300 Hz to 10 kHz) recording units, 32 local-field-potential (LFP, bandwidth: 1 Hz to 300 Hz) recording units, 32 current recording units, 32 impedance measurement units, and 28 neurotransmitter detection units, in addition to the 16 dual-mode voltage-only or current/voltage-controlled stimulation units. The electrode array architecture is based on a switch matrix, which allows for connecting any measurement/stimulation unit to any electrode in the array and for performing different measurement/stimulation functions in parallel.

Molecularly imprinted polymers with multi-functionality.

Molecular imprinting is a very powerful synthetic method for preparation of robust materials with pre-designed molecular selectivity. Molecularly imprinted polymers (MIPs) are attractive substitutes for antibodies in many analytical and bioanalytical applications, e.g. for development of biosensors and for drug assays. In addition to selective molecular binding, new functions are being added to MIPs to make the synthetic materials responsive to different environmental conditions, making it possible to modulate the binding and release of different molecular targets and to simplify affinity separation. Introduction of signal-transduction functionality into MIPs also brings in new, more easily operated chemical sensors for detection and quantification of important analytical targets.

Order, Disorder, and Everything in Between.

In addition to the "traditional" proteins characterized by the unique crystal-like structures needed for unique functions, it is increasingly recognized that many proteins or protein regions (collectively known as intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs)), being biologically active, do not have a specific 3D-structure in their unbound states under physiological conditions. There are also subtler categories of disorder, such as conditional (or dormant) disorder and partial disorder. Both the ability of a protein/region to fold into a well-ordered functional unit or to stay intrinsically disordered but functional are encoded in the amino acid sequence. Structurally, IDPs/IDPRs are characterized by high spatiotemporal heterogeneity and exist as dynamic structural ensembles. It is important to remember, however, that although structure and disorder are often treated as binary states, they actually sit on a structural continuum.

'I am an Intensive Guy': The Possibility and Conditions of Reconciliation Through the Ecological Intensification Framework.

The need for better conciliation between food production and environmental protection calls for new conceptual approaches in agronomy. Ecological intensification (EI) is one of the most encouraging and successful conceptual frameworks for designing more sustainable agricultural systems, though relying upon semantic ambivalences and epistemic tensions. This article discusses abilities and limits of the EI framework in the context of strong social and environmental pressure for agricultural transition. The purpose is thus to put EI at stake in the light of the results of an interdisciplinary and participatory research project that explicitly adopted EI goals in livestock semi-industrialized farming systems. Is it possible to maintain livestock production systems that are simultaneously productive, sustainable, and viable and have low nitrate emissions in vulnerable coastal areas? If so, how do local stakeholders use these approaches? The main steps of the innovation process are described. The effects of political and social dynamics on the continuity of the transition process are analyzed, with a reflexive approach. This experiment invites one to consider that making EI operational in a context of socio-technical transition toward agroecology represents system innovation, requiring on-going dialogue, reflexivity, and long-term involvement by researchers.

Conversion of farmland to forest or grassland improves soil carbon, nitrogen, and ecosystem multi-functionality in a subtropical karst region of southwest China.

The conversion of farmland to forest in China has been recognized for its positive impact on above-ground vegetation and carbon sequestration. However, the impact on soil quality during land conversion, particularly in vulnerable karst areas, has received less attention. In this study conducted in a karst area of southwest China, eight different farmland conversion strategies were investigated to assess improvements in surface soil carbon, nitrogen, and ecosystem multi-functionality (EMF). Our results showed that farmland converted to afforestation areas or farmland that was abandoned contained higher amounts of carbon (total, organic, active) and ammonium nitrogen (NH4+-N) in the soil compared to farmland converted to grassland or maize crop. Soluble organic carbon levels were higher in afforestation and grassland areas compared to maize crop controls. By contrast, soil from grassland and abandoned land exhibited higher levels of nitrate nitrogen (NO3--N) compared to afforestation land or maize crop controls. There were no differences in NH4+-N content between any condition, except for afforestation land that specifically contained the Zenia insignis plant species. Afforestation land consistently exhibited higher EMF values than grassland. Pearson correlation analysis revealed positive relationships between soil indices and EMF scores, except for NO3--N.Random forest analysis explained 95% of the variation in soil EMF and identified specific soil factors: total carbon, organic carbon, active labile organic carbon, total nitrogen, and ammonium nitrogen, as the main drivers of soil multi-functionality. Our studies show how various reforestation strategies can enhance soil nutrient sequestration and improve soil multi-functionality of farmland in the karst areas.These findings provide insight into sustainable soil management practices for converting farmland into natural areas.