Fitness benefits of alternated chick provisioning in cooperatively breeding carrion crows.

In most bird species, parents raise offspring cooperatively. In some cases, this cooperation extends to helpers-at-the-nest who assist the breeders with a range of tasks. While cooperative food provisioning might merely arise incidentally, as a result of the efforts of carers that act independently from each other, recent studies suggest that birds may coordinate by taking turns in visiting the nest. However, evidence that such coordination emerges because individuals actively respond to each other's behaviour is controversial, and the potential benefits of carers' alternation remain unknown. We addressed this knowledge gap by analysing a multiyear dataset for cooperatively breeding carrion crows Corvus corone, comprising 8693 nest visits across 50 groups. Our results reveal that turn-taking does occur in this species and that all group members, regardless of their sex and social role (breeder/helper), tend to alternate at the nest with other carers rather than to make repeat visits. Importantly, we found that the body mass of nestlings increased significantly with the degree of carers' alternation, possibly because well-coordinated groups provided food at more regular intervals. Using earlier monitoring data, the observed increase in body mass is predicted to substantially boost postfledging survival rates. Our analyses demonstrate that alternation in nestling provisioning has measurable fitness benefits in this study system. This raises the possibility that cooperatively breeding carrion crows, as well as other bird species with similarly coordinated brood provisioning, exhibit specialized behavioural strategies that enable effective alternation.

En la mayoría de las especies de aves, los padres cooperan entre sí en el cuidado de sus crías. En algunos casos, la cooperación se extiende a individuos ayudantes que asisten a los reproductores en diferentes tareas. El aprovisionamiento cooperativo de las crías puede darse simplemente como resultado del esfuerzo de cuidadores que actúan de forma independiente, pero estudios recientes sugieren que las aves pueden coordinarse, tomando turnos a la hora de visitar el nido. Sin embargo, las evidencias de que dicha coordinación ocurre porque los individuos realmente responden al comportamiento de los demás es controvertida, y los potenciales beneficios de la alternancia al nido son desconocidos. Nosotros abordamos estos aspectos analizando una base de datos plurianual en la corneja negra Corvus corone, que incluye 8693 visitas al nido en 50 grupos. Nuestros resultados revelan que se produce toma de turnos en esta especie y que todos los miembros del grupo, independientemente del sexo y del estatus social (reproductor / ayudante), tienden a alternarse al nido con otros cuidadores, en vez de repetir visitas. Más importante aún, encontramos que la masa corporal de los polluelos aumentaba de forma significativa al aumentar el grado de coordinación de los cuidadores, posiblemente porque los grupos mejor coordinados aprovisionaban las crías a intervalos más regulares. Basándonos en datos anteriores, pudimos calcular que el incremento observado en masa corporal predice un aumento sustancial de la tasa de supervivencia de los volanderos. Nuestros análisis demuestran, por lo tanto, que la alternancia al nido tiene beneficios medibles en eficacia biológica. Esto conlleva la posibilidad de que las cornejas negras cooperativas, al igual que otras especies de aves con aprovisionamiento coordinado de las crías, exhiban estrategias comportamentales especializadas que permitan una eficiente alternancia al nido.

Effects of cooling on thyroid hormone secretion and growth of eastern bluebird (Sialia sialis) nestlings.

Achieving endothermic homeothermy is a critical aspect of avian development. In pre-homeothermic altricial nestlings, variation in parental brooding behavior results in variable exposure of nestlings to cooling, with consequences for the developing endocrine system. Nestlings facing repeated cooling challenges may benefit from upregulation of thyroid hormone secretion, allowing for earlier onset of thermoregulatory capability to mitigate the potentially negative effects of exposure to non-optimal temperatures during development. We examined the effects of (1) a single cooling challenge on thyroid hormone secretion in pre-homeothermic nestlings, and (2) repeated cooling challenges prior to the onset of homeothermy on nestling growth and thyroid hormone secretion prior to fledging. We found that pre-homeothermic eastern bluebird nestlings exposed to a single cooling challenge increased circulating triiodothyronine (T3), demonstrating that the thyroid system can be activated by cooling early in life. However, we found no consequences of repeated cooling during the first week of life on nestling growth or baseline T3 levels prior to fledging. This work addresses how the nestling hypothalamic-pituitary-thyroid axis responds to acute cooling challenges prior to the development of endothermic homeothermy; future work will confirm whether such responses allow nestlings to hasten the onset of physiological thermoregulation when conditions demand it.

The effect of insect food availability on songbird reproductive success and chick body condition: Evidence from a systematic review and meta-analysis.

Reports of declines in abundance and biomass of insects and other invertebrates from around the world have raised concerns about food limitation that could have profound impacts for insectivorous species. Food availability can clearly affect species; however, there is considerable variation among studies in whether this effect is evident, and thus a lack of clarity over the generality of the relationship. To understand how decreased food availability due to invertebrate declines will affect bird populations, we conducted a systematic review and used meta-analytic structural equation modelling, which allowed us to treat our core variables of interest as latent variables estimated by the diverse ways in which researchers measure fecundity and chick body condition. We found a moderate positive effect of food availability on chick body condition and a strong positive effect on reproductive success. We also found a negative relationship between chick body condition and reproductive success. Our results demonstrate that food is generally a limiting factor for breeding songbirds. Our analysis also provides evidence for a consistent trade-off between chick body condition and reproductive success, demonstrating the complexity of trophic dynamics important for these vital rates.

Are brood sex ratios adaptive?-The effect of experimentally altered brood sex ratio on nestling growth, mortality and recruitment.

Brood sex ratios (BSRs) have often been found to be nonrandom in respect of parental and environmental quality, and many hypotheses suggest that nonrandom sex ratios can be adaptive. To specifically test the adaptive value of biased BSRs, it is crucial to disentangle the consequences of BSR and maternal effects. In multiparous species, this requires cross-fostering experiments where foster parents rear offspring originating from multiple broods, and where the interactive effect of original and manipulated BSR on fitness components is tested. To our knowledge, our study on collared flycatchers (Ficedula albicollis) is the first that meets these requirements. In this species, where BSRs had previously been shown to be related to parental characteristics, we altered the original BSR of the parents shortly after hatching by cross-fostering nestlings among trios of broods and examined the effects on growth, mortality and recruitment of the nestlings. We found that original and experimental BSR, as well as the interaction of the two, were unrelated to the fitness components considered. Nestling growth was related only to background variables, namely brood size and hatching rank. Nestling mortality was related only to hatching asynchrony. Our results therefore do not support that the observed BSRs are adaptive in our study population. However, we cannot exclude the possibility of direct effects of experimentally altered BSRs on parental fitness, which should be evaluated in the future. In addition, studies similar to ours are required on various species to get a clearer picture of the adaptive value of nonrandom BSRs.

Polymorphism at the nestling stage and host-specific mimicry in an Australasian cuckoo-host arms race.

Decades of research have shown that the coevolutionary arms race between avian brood parasites and their hosts can promote phenotypic diversification in hosts and brood parasites. However, relatively little is known about the role of brood parasitism in promoting phenotypic diversification of nestlings. We review field data collected over four decades in Australia, New Caledonia and New Zealand to assess potential for coevolutionary interactions between the shining bronze-cuckoo (Chalcites lucidus) and its hosts, and how diversification at the nestling stage may be generating different subspecies. The shining bronze-cuckoo is a specialist parasite of a few hosts in the family Acanthizidae. It has diversified into subspecies, of which the nestlings closely mimic the respective host nestlings in each region. Additionally, some cuckoo subspecies have polymorphic nestlings. The Acanthizidae hosts have similar breeding and nesting habits and only moderately effective frontline defences against parasitism at cuckoo egg laying or at the egg stages. However, some hosts have developed highly effective defences at the nestling stage by recognising and ejecting cuckoo nestlings from the nest. As with the cuckoo nestlings, some hosts have polymorphic nestlings. The coevolutionary interactions in each region suggest different evolutionary stages of the arms race in which either the parasite or the host is currently in the lead. The presence of moderately effective defences at the egg laying and egg stages might explain why some hosts do not have defences at the nestling stage. The south-Pacific cuckoo - host systems are excellent models to explore the evolutionary mechanisms driving the diversification at the nestling stage in the coevolutionary arms race between avian brood parasites and their hosts.

Responses of the gut microbiota to environmental heavy metal pollution in tree sparrow (Passer montanus) nestlings.

Gut microbiota plays a critical role in regulating the health and adaptation of wildlife. However, our understanding of how exposure to environmental heavy metals influences the gut microbiota of wild birds, particularly during the vulnerable and sensitive nestling stage, remains limited. In order to investigate the relationship between heavy metals and the gut microbiota, we analyzed the characteristics of gut microbiota and heavy metals levels in tree sparrow nestlings at different ages (6, 9 and 12-day-old). The study was conducted in two distinct areas: Baiyin (BY), which is heavily contaminated with heavy metals, and Liujiaxia (LJX), a relatively unpolluted area. Our result reveled a decrease in gut microbiota diversity and increased inter-individual variation among nestlings in BY. However, we also observed an increase in the abundance of bacterial groups and an up-regulation of bacterial metabolic functions associated with resistance to heavy metals toxicity in BY. Furthermore, we identified a metal-associated shift in the relative abundance of microbial taxa in 12-day-old tree sparrow nestlings in BY, particularly involving Aeromonadaceae, Ruminococcaceae and Pseudomonadaceae. Moreover, a significant positive correlation was found between the body condition of tree sparrow nestlings and the abundance of Bifidobacteriaceae in BY. Collectively, our findings indicate that the gut microbiota of tree sparrow nestlings is susceptible to heavy metals during early development. However, the results also highlight the presence of adaptive responses that enable them to effectively cope with environmental heavy metal pollution.

Adapting conservation breeding techniques using a data-driven approach to restore the 'Alala (Hawaiian crow, Corvus hawaiiensis).

For some critically endangered species, conservation breeding is a vital steppingstone toward re-establishing wild populations. The 'Alala (Hawaiian crow, Corvus hawaiiensis), currently extinct in the wild, exists today only in a conservation breeding program, which, for many years, utilized successful hands-on husbandry approaches such as separating and resocializing pairs, providing partially manmade nests, artificially incubating eggs, and puppet rearing nestlings. Yet, a top priority of any conservation breeding program is to retain natural behaviors essential to postrelease survival and reproduction, to achieve successful reintroduction and restoration to the wild. We describe how we are adapting 'Alala husbandry techniques to strengthen pair bonds through full-time socialization, enable pairs to build robust nests, encourage females to incubate eggs to hatch, and provide pairs and their offspring with vital parental rearing experiences. We discuss the use of standardized, data-driven methods to objectively track our progress towards successful parental breeding and to select release candidates based on their likelihood to survive and breed in the wild. The information shared in this report can be applied to other conservation breeding programs, particularly those implementing or transitioning to husbandry techniques geared towards preparing species to thrive in the wild.

The relative influence of cross-seasonal and local weather effects on the breeding success of a migratory songbird.

In seasonal environments, fluctuating early-season weather conditions and short breeding windows limit reproductive opportunities such that breeding earlier or later than the optimum may be particularly costly. Given the risk of early-season energy limitations, time- and energy-based carry-over effects stemming from environmental conditions across the annual cycle may have pronounced consequences for breeding phenology and fitness. Generally, when and where environmental conditions are most influential are poorly understood, limiting our ability to predict the future of climate-sensitive populations. For an alpine-breeding, migratory population of horned lark Eremophila alpestris in northern British Columbia, Canada (54.8°N), we assessed how weather conditions across the annual cycle influenced clutch initiation date and offspring development. We also addressed how cross-seasonal effects on breeding parameters combine to influence reproductive fitness. With 12 years of breeding data and 3 years of migration data, we used a sliding window approach to identify points during the annual cycle when weather events most influenced breeding phenology and offspring development. Consequences for breeding success were assessed using nest survival simulations. Average clutch initiation date varied up to 11 days among years but did not advance from 2003 to 2019. Warmer temperatures at stopover and breeding sites advanced clutch initiation, but winter conditions had no effect. Sub-zero stopover temperatures carried over to prolong offspring development independent of clutch initiation date, potentially indicating energy-based carry-over effects acting on parental investment. Nest survival decreased with both later clutch initiation and prolonged offspring development such that females nesting earlier and fledging offspring at a younger age were up to 45% more likely to reproduce successfully. We demonstrate that stronger carry-over effects originated from environmental conditions closer to the breeding site in time and space, as well as the potential for energy-based mechanisms to link pre-breeding conditions to reproductive fitness. We also highlight the importance of extended stopovers for songbirds breeding in seasonal environments, particularly given that climatic conditions are becoming increasingly decoupled across stages of the annual cycle. Understanding the cross-seasonal mechanisms shaping breeding decisions in stochastic environments allows for more accurate predictions of population-level responses to climate change.

Les variations saisonnières de l'environnement, notamment due aux conditions climatiques changeantes en début de saison ainsi que la réduction de la période propice à l'accouplement contraint fortement les possibilités de reproduction. Dans ces conditions, s'accoupler avant ou après le moment optimal peut s'avérer particulièrement coûteux. Les effets de report (temporels et énergétiques) causés par ces variations environnementales peuvent avoir des conséquences notables sur la phénologie de la reproduction, et ultimement sur la valeur sélective des individus. Où et quand les effets des conditions environnementales sont le plus critiques reste encore méconnu, limitant notre capacité à prédire le futur des populations sensibles aux variations climatiques. Ici, nous avons évalué comment des conditions climatiques au cours de l'année influencent la date de ponte et le développement des oisillons dans une population migratrice d'Alouette hausse-col Eremophila alpestris se reproduisant en milieux alpin. Nous tirons profit de données issues de 12 ans de suivit de la reproduction et de 3 ans de suivit migratoire, et utilisons une approche dite de 'sliding window' pour identifier les moments du cycle annuel pour lequel le climat a eu le plus d'influence sur la phénologie de la reproduction et le développement des oisillons. La date moyenne de ponte s'avère variable d'une année à l'autre (certaine différence allant jusqu'à 11 jours), mais ne se décale pas sur la période de 2003 à 2019. Nos résultats montrent que des conditions climatiques plus chaudes lors des haltes migratoires ainsi que sur les sites de reproductions rendent la date de ponte plus précoces. Des températures négatives lors des haltes migratoires aussi ont pour conséquences un temps de développement des oisillons plus long, et ceci indépendamment de la date de ponte. Cela suggère des effets de report, notamment énergétique, affectant l'investissement des parents. Nos résultats montrent que la survie au nid diminue lorsque la date de ponte est plus tardive ou que le temps de développement des oisillons est rallongé. De cette manière, les femelles commençants la nidification plus tôt et pour qui les oisillons quittent le nid plus tôt ont 45% plus de chance de se reproduire avec succès. Nous démontrons que des conditions environnementales proche du site de reproduction (que ce soit dans le temps ou dans l'espace) cause un fort effet de report, et suggérons un possible mécanisme reliant les conditions climatiques pré-reproductives au succès reproducteur. De plus, nous mettons en lumière l'importance des haltes migratoires prolongées pour la reproduction des passereaux en environnement saisonnier, particulièrement du fait que les conditions climatiques sont de plus en plus découplées au cours des les étapes du cycle annuel. Une meilleure compréhension des mécanismes inter-saisonniers influençant les décisions de reproduction en environnement stochastique permettrait de mieux prédire les réponses des populations aux changements climatiques.

Weather and food availability additively affect reproductive output in an expanding raptor population.

The joint effects of interacting environmental factors on key demographic parameters can exacerbate or mitigate the separate factors' effects on population dynamics. Given ongoing changes in climate and land use, assessing interactions between weather and food availability on reproductive performance is crucial to understand and forecast population dynamics. By conducting a feeding experiment in 4 years with different weather conditions, we were able to disentangle the effects of weather, food availability and their interactions on reproductive parameters in an expanding population of the red kite (Milvus milvus), a conservation-relevant raptor known to be supported by anthropogenic feeding. Brood loss occurred mainly during the incubation phase, and was associated with rainfall and low food availability. In contrast, brood loss during the nestling phase occurred mostly due to low temperatures. Survival of last-hatched nestlings and nestling development was enhanced by food supplementation and reduced by adverse weather conditions. However, we found no support for interactive effects of weather and food availability, suggesting that these factors affect reproduction of red kites additively. The results not only suggest that food-weather interactions are prevented by parental life-history trade-offs, but that food availability and weather conditions are crucial separate determinants of reproductive output, and thus population productivity. Overall, our results suggest that the observed increase in spring temperatures and enhanced anthropogenic food resources have contributed to the elevational expansion and the growth of the study population during the last decades.

Chill out: Environmentally relevant cooling challenge does not increase telomere loss during early life.

In vertebrates, exposure to diverse stressors during early life activates a stress response that can initiate compensatory mechanisms or promote cellular damage with long-term fitness consequences. A growing number of studies associate exposure to stressors during early life with increased damage to telomeres (i.e., promoting the shortening of these highly conserved, repeating sequences of non-coding DNA at chromosome ends). However, some studies show no such relationship, suggesting that the nature, timing, and context of these challenges may determine the degree to which physiological mediators of the stress response act in a damage-mitigating or damage promoting way in relation to telomere dynamics. In free-living eastern bluebirds (Sialia sialis), we have previously demonstrated that bouts of offspring cooling that occur when brooding females leave the nest increase at least one such physiological mediator of the stress response (circulating glucocorticoids), suggesting that variation in patterns of maternal brooding may result in different impacts on telomere dynamics at a young age. Here we experimentally tested whether repeated bouts of ecologically relevant offspring cooling affected telomere dynamics during post-natal development. Rates of telomere shortening during the nestling stage were not affected by experimental cooling, but they were affected by brood size and the rate of growth during the nestling stage. Our data suggest that the effects of developmental stress exposure on offspring telomeres are often context-dependent and that not all challenges that increase physiological mediators of stress result in damage to telomeres. Under some conditions, physiological mediators of stress may instead act as protective regulators, allowing for optimization of fitness outcomes in the face of environmental challenges.

Cooling increases corticosterone deposition in feathers of eastern bluebird chicks.

Exposure to noxious stimuli early in life can both activate and shape the development of the hypothalamic-pituitary-adrenal (HPA) axis in birds and other vertebrates, with the potential for lifelong consequences. Studies assessing early HPA axis activation often rely on collection of blood samples to evaluate circulating glucocorticoid levels. However, blood sampling in small altricial young is invasive, limited by animal size, and not sufficient to provide detailed information about hormone exposure over protracted periods of time. We tested the use of feather corticosterone as an alternative method to assess HPA axis activity early in life in free-living, altricial chicks, for whom timing of growth of first feathers coincides with a period of rapid growth, development of the HPA axis, and reliance on parental care. We investigated (1) whether ecologically relevant bouts of experimental cooling prior to the onset of homeothermy-conditions known to elevate circulating corticosterone-are reflected in changes of feather corticosterone deposition in Eastern bluebird (Sialia sialis) chicks, and (2) whether such changes occurred in a sex-dependent manner. We found that cooling during the first week of life resulted in elevated feather corticosterone in first-grown feathers of experimentally cooled chicks relative to controls. The timing of deposition of corticosterone in feathers in response to temperature treatments was delayed in females compared to males. Results indicate that the hormone deposition in feather tissues of altricial nestlings reflects exposure to environmental stimuli, and can thus provide a minimally invasive tool for assessing HPA activity in early life. The development of the HPA axis, or its activation in response to environmental stimuli early in life, may also occur in a sex-dependent manner in altricial birds.

Variation in feather corticosterone levels in Alpine swift nestlings provides support for the hypo-responsive hypothesis.

In birds, feather corticosterone values (CORTf) are increasingly used as a retrospective and integrative proxy of an individual's physiological state during the period of feather growth. Relatively high CORTf values are usually interpreted as an indicator of exposure to energy-demanding or stressful conditions during feather growth. However, in nestlings this interpretation might not always hold true. The reasons are that, firstly nestlings (especially altricial ones) still develop their hypothalamo-pituitary-adrenal (HPA) reactivity during the growth of their feathers. Hence, at a young age, nestlings might be unable to mount a substantial adrenocortical stress response. Secondly, some species are able to down-regulate their metabolism during food scarcity and therewith probably also their CORT release. Consequently, CORTf values may not unambiguously reflect whether nestlings have suffered from energy-demanding or stress situations. Relatively high CORTf values might indicate either energy-demanding or stressful conditions ('stress responsive hypothesis'), or - conversely - favourable conditions during the period of feather growth ('hypo-responsive hypothesis'). In the altricial Alpine swift (Tachymarptis melba), we tested which factors help to distinguish between the two hypotheses by considering factors which affect CORT release (brood size, weather) and factors which are affected by high CORT levels (nestling size and condition). We measured CORTf in 205 nestlings over 7 years and collected data on brood size, body size, body condition and prevailing weather. Nestling CORTf values were positively correlated with body condition and negatively with adverse weather, supporting the hypo-responsive hypothesis. Results from the Alpine swift study, supplemented with a survey of the literature, show that relatively easily collected parameters on brood size, nestling size and condition, and environmental factors can help to distinguish between the two hypotheses. A meaningful interpretation of nestling CORTf should only be made in the context of species-specific traits.

Opportunistic myiasis of nestling common blackbirds by Lucilia silvarum.

Previously, the Marsh greenbottle blowfly, Lucilia silvarum Meigen (Diptera: Calliphoridae), has been implicated in myiasis of anurans but not of mammals or birds. This report describes an incident of opportunistic myiasis of nestling common blackbirds, Turdus merula (Passeriformes: Turdidae), by L. silvarum larvae in association with an infestation by the hematophagous maggot Neottiophilum praeustum (Diptera: Piophilidae).

A single GnRH challenge promotes paternal care, changing nestling growth for one day.

Decades of comparative and experimental work suggest that testosterone (T) promotes mating effort at the expense of parental effort in many vertebrates. There is abundant evidence that T-mediated trade-offs span both evolutionary and seasonal timescales, as T is often higher in species or breeding stages with greater mating competition and lower in association with parental effort. However, it is less clear whether transient elevations in T within a male's own reactive scope can affect parental effort in the same way, with effects that are visible to natural selection. Here, we injected free-living male tree swallows (Tachycineta bicolor) with gonadotropin-releasing hormone (GnRH), thus temporarily maximizing T production within an individual's own limit. Passive loggers at each nest showed that GnRH-injected males provisioned more frequently than saline males for the subsequent day, and their offspring gained more mass during that time. The degree of offspring growth was positively correlated with the father's degree of T elevation, but provisioning was not proportional to changes in T, and GnRH- and saline-injected males did not differ in corticosterone secretion. These results suggest that prior knowledge of T-mediated trade-offs garnered from seasonal, evolutionary, and experimental research cannot necessarily be generalized to the timescale of transient fluctuations in T secretion within an individual. Instead, we propose that GnRH-induced T fluctuations may not result in visible trade-offs if selection has already sculpted an individual male's reactive scope based on his ability to handle the competing demands of mating and parental care.

Pulses of anthropogenic food availability appear to benefit parents, but compromise nestling growth in urban red-winged starlings.

The provision of anthropogenic food undoubtedly influences urban bird fitness. However, the nature of the impact is unclear, with both benefits and costs of urban diets documented. Moreover, the influence of short-term fluctuations in food availability, linked to urban weekday/weekend cycles of human presence, is largely unknown. We explored whether breeding red-winged starlings Onychognathus morio in Cape Town, South Africa, altered foraging and provisioning behaviour between days with high human presence (HHP) and days with low human presence (LHP)-i.e. weekdays versus weekends and vacation days. We investigated the relationship between starling diet, adult body mass and nestling development. Breeding adults consumed and provisioned the same quantity of food, but a significantly greater proportion of anthropogenic food on HHP compared to LHP days. Adults apparently benefited from the anthropogenic diet, experiencing significantly greater mass gain on HHP days. However, nestlings experienced a cost, with the number of HHP days during the nestling period associated negatively with nestling size. Adults may, therefore, benefit from the high calorie content of anthropogenic food, while nestlings may be negatively affected by nutrient limitation. The quantity of food available in urban environments may, therefore, benefit adult survival, while its quality imposes a cost to nestling growth.

Chronic noise exposure has context-dependent effects on stress physiology in nestling Tree Swallows (Tachycineta bicolor).

Anthropogenic noise is increasing in intensity and scope, resulting in changes to acoustic landscapes and largely negative effects on a range of species. In birds, noise can mask acoustic signals used in a variety of communication systems, including parent-offspring communication. As a result, nestling birds raised in noise may have challenges soliciting food from parents and avoiding detection by predators. Given that passerine nestlings are confined to a nest and therefore cannot escape these challenges, noise may also act as a chronic stressor during their development. Here, we raised Tree Swallow (Tachycineta bicolor) nestlings with or without continuous, white noise to test whether noise exposure affected baseline and stress-induced plasma, integrated feather corticosterone levels, and immune function. Stress physiology and immune function may also vary with the competitive environment during development, so we also examined whether noise effects varied with brood size and nestling mass. We found that overall, exposure to noise did not alter nestling stress physiology or immune function. However, light nestlings raised in noise exhibited lower baseline plasma and integrated feather corticosterone than heavy nestlings, suggesting alternative physiological responses to anthropogenic stimuli. Furthermore, light nestlings in larger broods had reduced PHA-induced immune responses compared to heavy nestlings, and PHA-induced immune responses were associated with higher levels of baseline plasma and feather CORT. Overall, our findings suggest that noise can alter the stress physiology of developing birds; however, these effects may depend on developmental conditions and the presence of other environmental stressors, such as competition for resources. Our findings may help to explain why populations are not uniformly affected by noise.

Experimental Amelioration of Harsh Weather Speeds Growth and Development in a Tropical Montane Songbird.

AbstractOrganisms living at high elevations generally grow and develop more slowly than those at lower elevations. Slow montane ontogeny is thought to be an evolved adaptation to harsh environments that improves juvenile quality via physiological trade-offs. However, slower montane ontogeny may also reflect proximate influences of harsh weather on parental care and offspring development. We experimentally heated and protected nests from rain to ameliorate harsh montane weather conditions for mountain blackeyes (Chlorocharis emiliae), a montane songbird living at approximately 3,200 m asl in Malaysian Borneo. This experiment was designed to test whether cold and wet montane conditions contribute to parental care and postnatal growth and development rates at high elevations. We found that parents increased provisioning and reduced time spent warming offspring, which grew faster and departed the nest earlier compared with offspring from unmanipulated nests. Earlier departure reduces time-dependent predation risk, benefitting parents and offspring. These plastic responses highlight the importance of proximate weather contributions to broad patterns of montane ontogeny and parental care.

Food availability limits avian reproduction in the city: An experimental study on great tits Parus major.

The altered ecological and environmental conditions in towns and cities strongly affect demographic traits of urban animal populations, for example avian reproductive success is often reduced. Previous work suggests that this is partly driven by low insect availability during the breeding season, but robust experimental evidence that supports this food limitation hypothesis is not yet available. We tested core predictions of the food limitation hypothesis using a controlled experiment that provided supplementary insect food (nutritionally enhanced mealworms supplied daily to meet 40%-50% of each supplemented brood's food requirements) to great tit nestlings in urban and forest habitats. We measured parental provisioning rates and estimated the amount of supplementary food consumed by control and experimental nestlings, and assessed their body size and survival rates. Provisioning rates were similar across habitats and control and supplemented broods, but supplemented (and not control) broods consumed large quantities of supplementary food. As predicted by the food limitation hypothesis we found that nestlings in (a) urban control broods had smaller body size and nestling survival rates than those in forest control broods; (b) forest supplemented and control broods had similar body size and survival rates; (c) urban supplemented nestlings had larger body size and survival rates than those in urban control broods; and crucially (d) urban supplemented broods had similar body size and survival rates to nestlings in forest control broods. Our results provide rare experimental support for the strong negative effects of food limitation during the nestling rearing period on urban birds' breeding success. Furthermore, the fact that supplementary food almost completely eliminated habitat differences in survival rate and nestling body size suggest that urban stressors other than food shortage contributed relatively little to the reduced avian breeding success. Finally, given the impacts of the amount of supplementary food that we provided and taking clutch size differences into account, our results suggest that urban insect populations in our study system would need to be increased by a factor of at least 2.5 for urban and forest great tits to have similar reproductive success.

Family matters: skin microbiome reflects the social group and spatial proximity in wild zebra finches.

BACKGROUND: So far, large numbers of studies investigating the microbiome have focused on gut microbiota and less have addressed the microbiome of the skin. Especially in avian taxa our understanding of the ecology and function of these bacteria remains incomplete. The involvement of skin bacteria in intra-specific communication has recently received attention, and has highlighted the need to understand what information is potentially being encoded in bacterial communities. Using next generation sequencing techniques, we characterised the skin microbiome of wild zebra finches, aiming to understand the impact of sex, age and group composition on skin bacteria communities. For this purpose, we sampled skin swabs from both sexes and two age classes (adults and nestlings) of 12 different zebra finch families and analysed the bacterial communities. RESULTS: Using 16S rRNA sequencing we found no effect of age, sex and family on bacterial diversity (alpha diversity). However, when comparing the composition (beta diversity), we found that animals of social groups (families) harbour highly similar bacterial communities on their skin with respect to community composition. Within families, closely related individuals shared significantly more bacterial taxa than non-related animals. In addition, we found that age (adults vs. nestlings) affected bacterial composition. Finally, we found that spatial proximity of nest sites, and therefore individuals, correlated with the skin microbiota similarity. CONCLUSIONS: Birds harbour very diverse and complex bacterial assemblages on their skin. These bacterial communities are distinguishable and characteristic for intraspecific social groups. Our findings are indicative for a family-specific skin microbiome in wild zebra finches. Genetics and the (social) environment seem to be the influential factors shaping the complex bacterial communities. Bacterial communities associated with the skin have a potential to emit volatiles and therefore these communities may play a role in intraspecific social communication, e.g. via signalling social group membership.

Higher Nest Predation Favors Rapid Fledging at the Cost of Plumage Quality in Nestling Birds.

High predation risk can favor rapid offspring development at the expense of offspring quality. Impacts of rapid development on phenotypic quality should be most readily expressed in traits that minimize fitness costs. We hypothesize that ephemeral traits that are replaced or repaired after a short period of life might express trade-offs in quality as a result of rapid development more strongly than traits used throughout life. We explored this idea for plumage quality in nestling body feathers, an ephemeral trait. We found a strong trade-off whereby nestlings that spend less time in the nest produced lower-quality plumage with less dense barbs relative to adults across 123 temperate and tropical species. For a subset of these species ( n=67 ), we found that variation in the risk of nest predation explained additional variation in plumage quality beyond development time. Ultimately, the fitness costs of a poor-quality ephemeral trait, such as nestling body feathers, may be outweighed by the fitness benefits of shorter development times that reduce predation risk. At the same time, reduced resource allocation to traits with small fitness costs, such as ephemeral traits, may ameliorate resource constraints from rapid development on traits with larger fitness impacts.