A new lineage of non-photosynthetic green algae with extreme organellar genomes.

BACKGROUND: The plastid genomes of the green algal order Chlamydomonadales tend to expand their non-coding regions, but this phenomenon is poorly understood. Here we shed new light on organellar genome evolution in Chlamydomonadales by studying a previously unknown non-photosynthetic lineage. We established cultures of two new Polytoma-like flagellates, defined their basic characteristics and phylogenetic position, and obtained complete organellar genome sequences and a transcriptome assembly for one of them. RESULTS: We discovered a novel deeply diverged chlamydomonadalean lineage that has no close photosynthetic relatives and represents an independent case of photosynthesis loss. To accommodate these organisms, we establish the new genus Leontynka, with two species (L. pallida and L. elongata) distinguishable through both their morphological and molecular characteristics. Notable features of the colourless plastid of L. pallida deduced from the plastid genome (plastome) sequence and transcriptome assembly include the retention of ATP synthase, thylakoid-associated proteins, the carotenoid biosynthesis pathway, and a plastoquinone-based electron transport chain, the latter two modules having an obvious functional link to the eyespot present in Leontynka. Most strikingly, the ~362 kbp plastome of L. pallida is by far the largest among the non-photosynthetic eukaryotes investigated to date due to an extreme proliferation of sequence repeats. These repeats are also present in coding sequences, with one repeat type found in the exons of 11 out of 34 protein-coding genes, with up to 36 copies per gene, thus affecting the encoded proteins. The mitochondrial genome of L. pallida is likewise exceptionally large, with its >104 kbp surpassed only by the mitogenome of Haematococcus lacustris among all members of Chlamydomonadales hitherto studied. It is also bloated with repeats, though entirely different from those in the L. pallida plastome, which contrasts with the situation in H. lacustris where both the organellar genomes have accumulated related repeats. Furthermore, the L. pallida mitogenome exhibits an extremely high GC content in both coding and non-coding regions and, strikingly, a high number of predicted G-quadruplexes. CONCLUSIONS: With its unprecedented combination of plastid and mitochondrial genome characteristics, Leontynka pushes the frontiers of organellar genome diversity and is an interesting model for studying organellar genome evolution.

Biotechnological Advances in the Design of Algae-Based Biosensors.

In addition to their use in biomass production and bioremediation, algae have been extensively exploited in biosensing applications. Algae-based biosensors have demonstrated potential for sensitive, sustainable, and multiplexed detection of analytes of agroenvironmental and security interest. Their advantages include the availability of different algal bioreceptors including whole cells and their photosynthetic subcomponents, their potential to be integrated into dual transduction miniaturized devices, and the opportunity for continuous environmental monitoring. Despite obstacles including limited stability and selectivity, algae-based biosensing is a realistic prospect that has some recent effective applications. Strategic exploitation of cutting-edge technologies including materials science, nanotechnology, microfluidics, and genome editing will help to achieve the full potential of algae-based sensors.

Carbon dioxide sequestration accompanied by bioenergy generation using a bubbling-type photosynthetic algae microbial fuel cell.

This study developed a bubbling-type photosynthetic algae microbial fuel cell (B-PAMFC) to treat synthetic wastewater and capture CO2 using Chlorella vulgaris with simultaneous power production. The performance of B-PAMFC in CO2 fixation and bioenergy production was compared with the photosynthetic algae microbial fuel cell (PAMFC) and bubbling photobioreactor. Different nitrogen sources for C. vulgaris growth, namely sodium nitrate, urea, ammonium acetate and acetamide were studied. The maximum CO2 fixation rate in B-PAMFC with 2.8 g L-1 urea reached 605.3 mg L-1 d-1, 3.86-fold higher than that in PAMFC. Urea also enhanced the solution absorption of CO2. Furthermore, the B-PAMFC reached a high lipid productivity of 105.9 mg L-1 d-1. An energy balance analysis indicated that B-PAMFC had a maximum net energy of 1.824 kWh m-3, making it a lab-scale energy-positive system. The B-PAMFC with urea as nitrogen source would provide an attractive strategy for simultaneous CO2 sequestration and bioenergy production.