NIR-responsive electrospun nanofiber dressing promotes diabetic-infected wound healing with programmed combined temperature-coordinated photothermal therapy.

BACKGROUND: Diabetic wounds present significant challenges, specifically in terms of bacterial infection and delayed healing. Therefore, it is crucial to address local bacterial issues and promote accelerated wound healing. In this investigation, we utilized electrospinning to fabricate microgel/nanofiber membranes encapsulating MXene-encapsulated microgels and chitosan/gelatin polymers. RESULTS: The film dressing facilitates programmed photothermal therapy (PPT) and mild photothermal therapy (MPTT) under near-infrared (NIR), showcasing swift and extensive antibacterial and biofilm-disrupting capabilities. The PPT effect achieves prompt sterilization within 5 min at 52 °C and disperses mature biofilm within 10 min. Concurrently, by adjusting the NIR power to induce local mild heating (42 °C), the dressing stimulates fibroblast proliferation and migration, significantly enhancing vascularization. Moreover, in vivo experimentation successfully validates the film dressing, underscoring its immense potential in addressing the intricacies of diabetic wounds. CONCLUSIONS: The MXene microgel-loaded nanofiber dressing employs temperature-coordinated photothermal therapy, effectively amalgamating the advantageous features of high-temperature sterilization and low-temperature promotion of wound healing. It exhibits rapid, broad-spectrum antibacterial and biofilm-disrupting capabilities, exceptional biocompatibility, and noteworthy effects on promoting cell proliferation and vascularization. These results affirm the efficacy of our nanofiber dressing, highlighting its significant potential in addressing the challenge of diabetic wounds struggling to heal due to infection.

A magnetic nanozyme platform for bacterial colorimetric detection and chemodynamic/photothermal synergistic antibacterial therapy.

Rapid, convenient, and sensitive detection of bacteria and development of novel antibacterial materials are conducive to accurate treatment of bacterial infection and reducing the generation of drug-resistant bacteria caused by overuse of antibiotics. A dual-function magnetic nanozyme, Fc-MBL@rGO@Fe3O4, has been constructed with broad-spectrum bacterial affinity and good peroxidase-like activity. Detection signal amplification was realized in the presence of 3,3',5,5'-tetramethylbenzidine (TMB) with a detection limit of 26 CFU/mL. In addition, the excellent photothermal properties of Fc-MBL@rGO@Fe3O4 could realize synergistic chemodynamic/photothermal antibacterial therapy. Furthermore, the good bacterial affinity of Fc-MBL@rGO@Fe3O4 enhances the accurate and rapid attack of hydroxyl radical (·OH) on the bacterial membrane and achieves efficient sterilization (100%) at low concentration (40 µg/mL) and mild temperature (47℃). Notably, Fc-MBL@rGO@Fe3O4 has a broad spectrum of antibacterial activity against Gram-negative, Gram-positive, and drug-resistant bacteria. The magnetic nanoplatform integrating detection-sterilization not only meets the need for highly sensitive and accurate detection in different scenarios, but can realize low power density NIR-II light-responsive chemodynamic/photothermal antibacterial therapy, which has broad application prospects.

A Processible and Ultrahigh-temperature Organic Photothermal Material through Spontaneous and Quantitative [2+2] Cycloaddition-Cycloreversion.

Energy conversion, particularly light to heat conversion, has garnered significant attention owing to its prospect in renewable energy exploitation and utilization. Most previous efforts have focused on developing organic photothermal materials for low-temperature applications, whereas the importance of simplifying the preparation methods of photothermal materials and enhancing their maximum photothermal temperature have been less taken. Herein, we prepare an organic near-infrared (NIR) photothermal material namely ATT by a spontaneous [2+2] cycloaddition-cycloreversion reaction. In addition to the solution-based method, ATT could also be readily preapred by ball milling in a high yield of 90 % in just 15 min. ATT powder exhibits a broad absorption extending beyond 2000 nm, excellent processability, and thermal stability. Remarkably, ATT powder can reach an unprecedently temperature as high as 450 °C while maintaining excellent photostability upon photoirradiation. Leveraging its extraordinary photothermal and processable properties, ATT was used in the high-temperature applications, such as photo-ignition, photo-controlled metal processing and high-temperature shape memory, all of which offer spatiotemporal control capabilities. This work provides a new approach to prepare organic photothermal materials with high temperatures, and pave the way for their applications in extreme environments.

New Insight into Assembled Fe3O4@PEI@Ag Structure as Acceptable Agent with Enzymatic and Photothermal Properties.

Metal-based enzyme mimics are considered to be acceptable agents in terms of their biomedical and biological properties; among them, iron oxides (Fe3O4) are treated as basement in fabricating heterogeneous composites through variable valency integrations. In this work, we have established a facile approach for constructing Fe3O4@Ag composite through assembling Fe3O4 and Ag together via polyethyleneimine ethylenediamine (PEI) linkages. The obtained Fe3O4@PEI@Ag structure conveys several hundred nanometers (~150 nm). The absorption peak at 652 nm is utilized for confirming the peroxidase-like activity of Fe3O4@PEI@Ag structure by catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. The Michaelis-Menten parameters (Km) of 1.192 mM and 0.302 mM show the higher catalytic activity and strong affinity toward H2O2 and TMB, respectively. The maximum velocity (Vmax) value of 1.299 × 10-7 M·s-1 and 1.163 × 10-7 M·s-1 confirm the efficiency of Fe3O4@PEI@Ag structure. The biocompatibility illustrates almost 100% cell viability. Being treated as one simple colorimetric sensor, it shows relative selectivity and sensitivity toward the detection of glucose based on glucose oxidase. By using indocyanine green (ICG) molecule as an additional factor, a remarkable temperature elevation is observed in Fe3O4@PEI@Ag@ICG with increments of 21.6 ∘C, and the absorption peak is nearby 870 nm. This implies that the multifunctional Fe3O4@PEI@Ag structure could be an alternative substrate for formatting acceptable agents in biomedicine and biotechnology with enzymatic and photothermal properties.

Metal-polyphenol coordination nanosheets with synergistic peroxidase-like and photothermal properties for efficient antibacterial treatment.

Bacterial infections pose a serious threat to human health and socioeconomics worldwide. In the post-antibiotic era, the development of novel antimicrobial agents remains a challenge. Polyphenols are natural compounds with a variety of biological activities such as intrinsic antimicrobial activity and antioxidant properties. Metal-polyphenol obtained by chelation of polyphenol ligands with metal ions not only possesses efficient antimicrobial activity but also excellent biocompatibility, which has great potential for application in biomedical and food packaging fields. Herein, we developed metal-polyphenol coordination nanosheets named copper oxidized tannic acid quinone (CuTAQ) possessing efficient antibacterial and anti-biofilm effects, which was synthesized by a facile one-pot method. The synthesis was achieved by chelation of partially oxidized tannic acid (TA) with Cu2+ under mild conditions, which supports low-cost and large-scale production. It was demonstrated that CuTAQ exhibited high antibacterial activity via disrupting the integrity of bacterial cell membranes, inducing oxidative stress, and interfering with metabolism. In addition, CuTAQ exhibits excellent peroxidase catalytic activity and photothermal conversion properties, which play a significant role in enhancing its bactericidal and biofilm scavenging abilities. This study provides insights for rational design of innovative metal-polyphenol nanomaterials with efficient antimicrobial properties.

Three-dimensional porous manganese oxide/nickel/carbon microspheres as high-performance electromagnetic wave absorbers with superb photothermal property.

Regulating electromagnetic parameters and thus improving impedance matching characteristics by multi-component design is regarded as a prospective approach to obtain highly efficient electromagnetic wave absorption materials. Whereas, it is still challenging to fabricate microwave absorbers with strong absorption capacity and durability in harsh conditions. Based on the above considerations, three-dimensional porous multi-functional manganese oxide/nickel/carbon microspheres had been designed and prepared through a combined approach of facile solvothermal reactions and subsequent carbonization processes. The textural characteristic examinations demonstrated that, numerous manganese oxide and Ni nanoparticles of 15-20 nm in diameter were well dispersed in the carbon-based microspheres of approximately 0.8-1 µm in size. Microwave absorption property evaluation indicated that the minimum reflection loss reached up to -53.6 dB at 9.5 GHz, and effective absorption bandwidth of 3.7 GHz was achieved at matching thickness of merely 2.0 mm. The electromagnetic wave attenuation mechanisms analysis displayed that excellent impedance matching and various dissipation pathways, including magnetic loss, interfacial and dipole polarization relaxation synergistically contributed to the high microwave absorption performances of the porous composites. Radar cross-sectional simulation and photothermal measurements verified that the materials were supposed to have promising foregrounds in complicated circumstances.

Doxorubicin-Loaded Fungal-Carboxymethyl Chitosan Functionalized Polydopamine Nanoparticles for Photothermal Cancer Therapy.

In this work, we synthesized doxorubicin-loaded fungal-carboxymethyl chitosan (FC) functionalized polydopamine (Dox@FCPDA) nanoparticles for improved anticancer activity via photothermal drug release. The photothermal properties revealed that the FCPDA nanoparticles with a concentration of 400 µg/mL produced a temperature of about 61.1 °C at 2 W/cm2 laser illumination, which is more beneficial for cancer cells. Due to the hydrophilic FC biopolymer, the Dox was successfully encapsulated into FCPDA nanoparticles via electrostatic interactions and pi-pi stacking. The maximum drug loading and encapsulation efficiency were calculated to be 19.3% and 80.2%, respectively. The Dox@FCPDA nanoparticles exhibited improved anticancer activity on HePG2 cancer cells when exposed to an NIR laser (800 nm, 2 W/cm2). Furthermore, the Dox@FCPDA nanoparticles also improved cellular uptake with HepG2 cells. Therefore, functionalizing FC biopolymer with PDA nanoparticles is more beneficial for drug and photothermal dual therapeutic properties for cancer therapy.

Facile Preparation of Hydrogel-Coated Surfaces with Antifouling and Salt Resistance for Efficient Solar-Driven Water Evaporation.

Hydrogel-based evaporators are a promising strategy to obtain freshwater from seawater and sewage. However, the time-consuming and energy-consuming methods used in hydrogel preparation, as well as their limited scalability, are major factors that hinder the development of a hydrogel-based evaporator. Herein, a facile and scalable strategy was designed to prepare a hydrogel-coated evaporator to realize efficient solar-driven water evaporation. The hydrogel coating layer is composed of a robust 3D network formed by tannic acid (TA) and poly(vinyl alcohol) (PVA) through a hydrogen bond. With the assistance of TA surface modifier, carbon black (CB) is uniformly distributed within the hydrogel matrix, endowing the coating with remarkable photothermal properties. In addition, Fe3+ is deposited on the surface of the hydrogel coating through metal coordination with TA, further improving the light absorption of the coating. Due to the synergistic effect of CB and Fe3+, the hydrogel-coated foam exhibited excellent photothermal properties. The water evaporation rate reached 3.64 kg m-2 h-1 under 1 sun irradiation. Because of the hydration ability of PVA hydrogel and the large porous structure of the foam, the hydrogel-coated foam demonstrated excellent antifouling performance and salt resistance. This study provides a facile method for designing and manufacturing high-performance solar-driven water evaporation materials.

Near-Infrared Remotely Controllable Shape Memory Biodegradable Occluder Based on Poly(l-lactide-co-ε-caprolactone)/Gold Nanorod Composite.

Biodegradable occluders, which can efficiently eliminate the complications caused by permanent foreign implants, are considered to be the next-generation devices for the interventional treatment of congenital heart disease. However, the controllability of the deployment process of degradable occluders remains a challenge. In this work, a near-infrared (NIR) remotely controllable biodegradable occluder is explored by integrating poly(l-lactide-co-ε-caprolactone) (PLCL) with poly(ethylene glycol)-modified gold nanorods (GNR/PEG). The caprolactone structural units can effectively increase the toughness of poly(l-lactide) and reduce the shape-memory transition temperature of the occluder to a more tissue-friendly temperature. Gold nanorods endow the PLCL-GNR/PEG composite with an excellent photothermal effect. The obtained occluder can be easily loaded into a catheter for transport and spatiotemporally expanded under irradiation with near-infrared light to block the defect site. Both in vitro and in vivo biological experiments showed that PLCL-GNR/PEG composites have good biocompatibility, and the PEGylated gold nanorods could improve the hemocompatibility of the composites to a certain extent by enhancing their hydrophilicity. As a thermoplastic shape-memory polymer, PLCL-GNR/PEG can be easily processed into various forms and structures for different patients and lesions. Therefore, PLCL-GNR/PEG has the potential to be considered as a competitive biodegradable material not only for occluders but also for other biodegradable implants.

Structure-controlled lignin complex for PLA composites with outstanding antibacterial, fluorescent and photothermal conversion properties.

Polylactic acid (PLA) is increasingly being considered as an ideal biodegradable candidate to replace petroleum-based polymers. However, its practical applications are often hampered by the poor mechanical robustness and solo functionality. Herein, based on the mechanical property improvement of PLA we proposed a simple process of assembling lignin-hybridized modifier and PLA matrix, as opposed to the traditional trade-off between mechanical strength and functionality, while anchoring a biofluorescent moiety onto lignin surfaces. Specifically, the fluorophore group could act as interfacial compatibilizer of complex and facilitate the shape-tailored hybrids for promoting functionality flexibility. With the bimetallic hetero-particles, the preferable lignin-assembled complex could be controllably configured as an antibacterial, fluorophore and photothermal agent. Thus, mechanical enhancement, fluorescence introduction and favorable photothermal ability of the resulting PLA composites were successfully achieved for integrated unification of structural robustness, geometric integrity and functional multiplicity, which was never seen in the other reports. The results showed that PLA composites containing 5 wt% modified lignin, 10 wt% zinc oxide, and 5 wt% silver presented excellent mechanical, fluorescent, photothermal conversion properties. By controlling the ZnO content and morphology, strong inhibition of Escherichia coli (Gram-negative) than that of Staphylococcus Aureus (Gram-positive) was also observed. The flake-shaped ZnO /Ag hybrids contributed to better overall performance of PLA composites than the rod-shaped ZnO/Ag. In this work we developed a facile strategy to assemble a bioderived fluorophore with lignin particles for constructing a structure-controlled complex as a multitasking modifier, featuring mechanical unity and functional adaptability. Specifically, the lignin reinforcement and bimetallic hybrids with different morphologies were explored as an effective fluorophore, antibacterial and photothermal agent. Through multiple dehydration reactions, a conjugating fluorophore was successfully grafted on lignin surfaces to serve as an interface modifier without physical changing its structural robustness. And morphology-tailored hybrid was advantageously immobilized on predefined hetero-particle carrier of fluorescent lignin and endowed composites with desirable antimicrobial properties. The developed strategy would expand the functional applications of PLA materials in food packaging, biopharmaceuticals and simple fluorescent anti-counterfeiting.

NIR-regulated dual-functional silica nanoplatform for infected-wound therapy via synergistic sterilization and anti-oxidation.

Nature-derived bioactive components and photothermal synergistic therapy bring potential strategies for fighting bacterial infection and accelerating would healing by virtue of their excellent therapeutic efficiencies and ignorable side effects, where photothermal property not only acts as sterilization energy but also as a doorkeeper to control the natural component release. Herein, by integrating the excellent antibacterial property of cinnamaldehyde (CA) and the outstanding photothermal performance of copper sulfide nanoparticles (CuS NPs), a multifunctional nanoplatform of SiO2 @CA@CuS nanospheres (NSs) is constructed with silica nanosphere (SiO2 NSs) as carrier. SiO2 @CA@CuS NSs exhibit photothermal property, bacterial absorption capacity, extraordinary antibacterial activity and antioxidant property. Mechanism characteriazation and antibacterial experiment indicate that positive charged SiO2 @CA@CuS can adhere to the negative charged surface of bacteria, and quickly kill bacteria through the synergistic action of the released CA and heat produced under near infrared light (NIR) irradiation at 980 nm. The sterilization efficiencies for Escherichia coli (E. coli) and S. aureus reach 99.86% and 99.84%, respectively. Furthermore, NIR-regulated SiO2 @CA@CuS perform great biocompatibility, as well as effective effects for accelerating S. aureus-infected wound healing at a low photothermal temperature (45 °C) relying on synergistic sterilization and anti-oxidation.

A graphene assembled porous fiber-based Janus membrane for highly effective solar steam generation.

Owing to the shortage of clean water as the global problem, the exploration of photothermal substances with high performance solar steam generation for sustainable water purification is essential and urgent. Herein, we demonstrate the assembly of two-dimensional graphene into one-dimensional rough, loose, and porous fibers and further use the assembled fibers to fabricate Janus membrane evaporator. The specific configuration guarantees an enhanced light harvesting property through multiple reflections, and improves the vapor transport ability through the constructed interlaced network. As a result, the as-obtained evaporator exhibits high solar absorbance, superior photothermal property and energy conversion efficiency, which is much higher than those of other reported Janus membrane evaporators and also better than the fabricated carbon nanotube-, and graphene sheet-based Janus membrane evaporator. The water purification results indicate that the fabricated graphene fiber-based Janus membrane is highly effective in seawater desalination without obvious salt accumulation and heavy metal wastewater purification. This study proposes a neotype graphene assembly for the fabrication of Janus membrane evaporator, which has potential applications in desalination and wastewater decontamination.

Rough Carbon-Iron Oxide Nanohybrids for Near-Infrared-II Light-Responsive Synergistic Antibacterial Therapy.

Infections caused by multidrug resistant bacteria are still a serious threat to human health. It is of great significance to explore effective alternative antibacterial strategies. Herein, carbon-iron oxide nanohybrids with rough surfaces (RCF) are developed for NIR-II light-responsive synergistic antibacterial therapy. RCF with excellent photothermal property and peroxidase-like activity could realize synergistic photothermal therapy (PTT)/chemodynamic therapy (CDT) in the NIR-II biowindow with improved penetration depth and low power density. More importantly, RCF with rough surfaces shows increased bacterial adhesion, thereby benefiting both CDT and PTT through effective interaction between RCF and bacteria. In vitro antibacterial experiments demonstrate a broad-spectrum synergistic antibacterial effect of RCF against Gram-negative Escherichia coli (E. coli), Gram-positive Staphylococcus aureus (S. aureus), and methicillin-resistant Staphylococcus aureus (MRSA). In addition, satisfactory biocompatibility makes RCF a promising antibacterial agent. Notably, the synergistic antibacterial performances in vivo could be achieved employing the rat wound model with MRSA infection. The current study proposes a facile strategy to construct antibacterial agents for practical antibacterial applications by the rational design of both composition and morphology. RCF with low power density NIR-II light responsive synergistic activity holds great potential in the effective treatment of drug-resistant bacterial infections.

Controlled Release and Photothermal Behavior of Multipurpose Nanocomposite Particles Containing Encapsulated Gold-Decorated Magnetite and 5-FU in Poly(lactide-co-glycolide).

Nowadays, many research studies have been conducted to prepare multidisciplinary probes in drug delivery systems and cancer therapy with high performance and minimum side effects. Here, poly(lactide-co-glycolide) (PLGA) nanocomposite particles containing 5-fluorouracil (5-FU) and gold-decorated magnetite nanoparticles with a raspberry-like morphology were designed and prepared as a novel and anticancer probe. For this reason, Fe3O4 nanoparticles were synthesized by a coprecipitation method and modified with (3-mercaptopropyl) trimethoxysilane for the deposition of gold nanoparticles. Then, they were embedded in the PLGA matrix alone and accompanied by 5-FU with 92 and 88% loading efficiencies, respectively, through a multiple emulsion solvent evaporation method. Chemical structure and composition of the prepared samples in each step were completely characterized by several techniques. The morphology of the nanocomposite particles was assessed by field emission scanning electron microscopy, high-resolution transmission electron microscopy, and selected area electron diffraction patterns, and their particle size and colloidal stability after 1 week were evaluated by dynamic light scattering. Because of the coexistence of gold and Fe3O4 nanoparticles, the final probe provided enhanced dual magneto and photothermal responses by increasing the temperature up to 42.7 °C under 5 min external alternating magnetic field and to 42.1 °C within just 1 min near-infrared irradiation at 808 nm. Trypan blue dye exclusion assays showed that they are biocompatible with reasonable toxicity (IC50 of 0.62 mg/mL) with respect to DU145 prostate cancer cells. Drug release profile of the 5-FU-loaded nanocomposite particles demonstrated their controlled release at 37 °C in phosphate-buffered saline solution. These indicate multidisciplinary characteristics of such particles in cancer therapy by photothermal, magnetic hyperthermia, and chemotherapy according to the presence of various active components.

Intracellular Enzyme-Triggered Assembly of Amino Acid-Modified Gold Nanoparticles for Accurate Cancer Therapy with Multimode.

Multiple amino acid (glutamine and lysine)-modified gold nanoparticles a with pH-switchable zwitterionic surface were fabricated through coordination bonds using ferrous iron (Fe2+) as bridge ions, which are able to spontaneously and selectively assemble in tumor cells for accurate tumor therapy combining enzyme-triggered photothermal therapy and H2O2-dependent catalytic medicine. These gold nanoparticles showed electric neutrality at pH 7.4 (hematological system) to prevent endocytosis of normal cells, which could be positively charged at pH 6.8 (tumor microenvironment) to promote the endocytosis of tumor cells to these nanoparticles, performing great tumor selectivity. After cell uptake, the specific enzyme (transglutaminase) in tumor cells would catalyze the polymerization of glutamine and lysine to cause the intracellular assembly of these gold nanoparticles, resulting in an excellent photothermal property for accurate tumor therapy. Moreover, the Fe2+ ion could decompose excess hydrogen peroxide (H2O2) in tumor cells via the Fenton reaction, resulting in a large amount of hydroxyl radicals (·OH). These radicals would also cause tumor cell damage. This synergetic therapy associating with high tumor selectivity generated an 8-fold in vitro cytotoxicity against tumor cells compared with normal cells under 48 h incubation with 10 min NIR irradiation. Moreover, in vivo data from tumor-bearing nude mice models showed that tumors can be completely inhibited and gradually eliminated after multimode treatment combining catalytic medicine and photothermal therapy for 3 weeks. This system takes advantage of three tumor microenvironment conditions (low pH, enzyme, and H2O2) to trigger the therapeutic actions, which is a promising platform for cancer therapy that achieved prolonged circulation time in the blood system, selective cellular uptake, and accurate tumor therapy in multiple models.