1.
Chembiochem
; 17(15): 1416-20, 2016 08 03.
Artigo
em Inglês
| MEDLINE
| ID: mdl-27194395
RESUMO
We report a ß-hairpin dual stabilizing strategy: a d-proline-l-proline (d-Pro-l-Pro) dipeptide as the nucleating turn, and a thioether tether as a side-chain linkage at a precisely designed position to stabilize the ß-hairpin. This method was used to modify the C-terminal ß-hairpin moiety of the plant defensin, pv-defensin, in order to obtain a stabilized peptide with enhanced anti-Candida albicans activity (MIC 84-3.0â µm), high serum stability (50 % remaining after 48â h) and low hemolysis (<10 % at 152â µm). This modified peptide penetrated the C.â albicans cell membrane within 5â min and showed high activity against clinically isolated antibiotic-resistant C.â albicans and Candida glabrata strains.