Integrated Metabolome and Transcriptome Analysis Provides New Insights into the Glossy Graft Cucumber Fruit (Cucumis sativus L.).

Cucumber is an important vegetable crop, and grafts often affect the quality and wax loss in cucumber fruit and affect its value. However, their metabolites and molecular mechanisms of action remain unclear. Metabolome and transcriptome analyses were conducted on the fruit peels of self-rooted plants (SR) grafted with white seed pumpkin (WG). The results showed that there were 352 differential metabolites in the fruit peels of the SR and WG. The transcriptome analysis showed 1371 differentially expressed genes (DEGs) between the WG and SR. These differentially expressed genes were significantly enriched in plant hormone signal transduction, cutin, suberin, wax biosynthesis, phenylpropanoid biosynthesis, and zeatin biosynthesis. By analyzing the correlation between differential metabolites and differentially expressed genes, six candidate genes related to the synthesis of glycitein, kaempferol, and homoeriodictyol were identified as being potentially important. Key transcription factors belonging to the TCP and WRKY families may be the main drivers of transcriptional changes in the peel between the SR and WG. The results of this study have provided a basis for the biosynthesis and regulation of wax loss and quality in grafted cucumbers and represents an important step toward identifying the molecular mechanisms of grafting onto cucumber fruit.

Preparation and clinical application of a nose prosthesis by means of selective laser simtering and wax powder / 中华医学美学美容杂志

Objective To explore the clinical application of reconstructing silicone elastomer nose prosthesis by means of selected laser sintering and wax powder PCPI. Methods Laser scanning was used to get the 3-D data of a nose model. Surfacere 10.0 etc softwares was used to reconstruct the nose by mirroring the digitalized model of absent nose. Selective laser sintering and wax powder was chosen to fabricate a wax nose model and the nose prosthesis made by silicone clastomer. Results Perfect silicone clastomer nose prosthesis was made for 2 patients. Conclusion This study suggests that the wax nose model and the new wax powder can meet the requirement of clinical expectation for maxillofacial prosthesis.

Fabrication of a nose prosthesis by means of selective laser sintering and wax powder / 实用口腔医学杂志

Objective:To reconstruct a 3-D wax nose model and make a s ilicone elastomer nose prosthesis. Methods:Laser scanning was us ed to get the data of a nose model, selective laser sintering and wax powder wer e used to fabricate a wax nose model,the nose prosthesis was made by silicone el astomer. The differences in length(L),width(W),deepth(D),height(H) and tip-angl e(TA) among the nose models made with plaster, wax powder and silicone elastomer were compared. Results:L(mm) in plaster, wax and silicon e models was 36.61,36.60 and 36.60 respectively.W(mm),D(mm),H(mm) and TA(?) in the three kinds of models were the same:36.23,18.45,43.14 and 74.57 respectively .Conclusion:Nose model made of the wax powder is precise and can meet the requirements for maxillofacial prosthesis.