RESUMO
Threose nucleic acid (TNA) is refractory to nuclease digestion and capable of undergoing Darwinian evolution, which together make it a promising system for diagnostic and therapeutic applications that require high biological stability. Expanding the sequence/chemical diversity of TNA would enable the development of functional TNA molecules with enhanced physicochemical properties. Recently, we have reported the synthesis and polymerase activity of a fluorescent cytidine TNA triphosphate analogue (1,3-diaza-2-oxo-phenothiazine, tCfTP) that maintains Watson-Crick base pairing with guanine. It not only improves TNA synthesis by enabling an engineered TNA polymerase to read through sequential G-repeats in a DNA template but it also introduces new physicochemical properties, such as increased hydrophobic character and fluorescence. Here, we describe the synthesis protocol for tCfTP, which includes three silica gel purifications, two precipitations, and one HPLC purification. Starting from protected threofuranosyl sugar, desired TNA nucleoside was obtained in a Vorbrüggen glycosylation reaction. After deprotection, nucleoside was further converted to 3'-monophosphate, activated by 2-methylimidazole, and subsequently treated with pyrophosphate to afford the desired 3'-triphosphate (tCfTP).
Assuntos
DNA Polimerase Dirigida por DNA/metabolismo , DNA/química , Corantes Fluorescentes/química , Ácidos Nucleicos/química , Tetroses/síntese química , Tetroses/químicaRESUMO
This unit describes the application of "synthetic genetics," i.e., the replication of xeno nucleic acids (XNAs), artificial analogs of DNA and RNA bearing alternative backbone or sugar congeners, to the directed evolution of synthetic oligonucleotide ligands (XNA aptamers) specific for target proteins or nucleic acid motifs, using a cross-chemistry selective exponential enrichment (X-SELEX) approach. Protocols are described for synthesis of diverse-sequence XNA repertoires (typically 1014 molecules) using DNA templates, isolation and panning for functional XNA sequences using targets immobilized on solid phase or gel shift induced by target binding in solution, and XNA reverse transcription to allow cDNA amplification or sequencing. The method may be generally applied to select fully-modified XNA aptamers specific for a wide range of target molecules. © 2018 by John Wiley & Sons, Inc.