RESUMO
BACKGROUND: CD163 is the monocyte/macrophage receptor for haptoglobin-haemoglobin complexes. The aim of this study was to assess the kinetics in the expression of CD163 on monocytes and the concentration of soluble sCD163 in serum of psoriatic patients in order to examine the effect of Goeckerman therapy. METHODS: sCD163 was measured in 71 patients before and after therapy, and in 57 healthy donors. A subgroup of 40 patients and 25 controls was used to assess the expression of membrane CD163. sCD163 was evaluated by ELISA. Flow cytometry method was used to determine the expression of membrane CD163 on monocytes, expressed as mean fluorescence index (MFI). RESULTS: Before therapy, the serum level of sCD163 was significantly higher in our patients than in controls (P=0.0154). However, we observed a profound decrease in sCD163 in our patients after therapy (P=0.0037). Similar to sCD163, pre-treatment expression of CD163 on monocytes was significantly more enhanced in patients than that in controls (P=0.0078). There was a trend towards down-regulation of the expression after therapy, nonetheless, the change was not statistically significant compared to the values before therapy (P=0.8666). This was also confirmed by comparison with controls which displayed lower expression of CD163 than patients after therapy (P=0.0019). The disease activity, expressed as PASI score, was significantly decreased in our patients by GT (P=0.0001). CONCLUSIONS: While sCD163 level in psoriatic patients was diminished after GT therapy, CD163 expression on monocytes was altered only to a minor extent.
Assuntos
Antígenos CD/sangue , Antígenos de Diferenciação Mielomonocítica/sangue , Alcatrão/uso terapêutico , Monócitos/metabolismo , Fotoquimioterapia , Psoríase/tratamento farmacológico , Receptores de Superfície Celular/sangue , Administração Cutânea , Adulto , Antígenos CD/fisiologia , Antígenos de Diferenciação Mielomonocítica/fisiologia , Antígenos de Superfície/análise , Biomarcadores , Alcatrão/administração & dosagem , Alcatrão/efeitos da radiação , Dano ao DNA , Ensaio de Imunoadsorção Enzimática , Feminino , Citometria de Fluxo , Humanos , Ativação de Macrófagos , Masculino , Pessoa de Meia-Idade , Psoríase/sangue , Psoríase/imunologia , Receptores de Superfície Celular/fisiologia , Índice de Gravidade de Doença , Solubilidade , Raios Ultravioleta , Adulto JovemRESUMO
Twenty kinds of coal-tar dyes were subjected to median tolerance limit (TLm) test by use of Himedaka (Oryzias latipes) for the comparision of their acute toxicities. It became clear that 4 kinds of halogens substituted xanthene compounds dyes showed strong acute toxicities. From the fact that uranine had the lower acute toxicity than halogens substituted compounds and the toxicities of these 4 dyes increased through irradiation, it was assumed that halogen atoms in dyes might be responsible for these strong acute toxicities to fish.