RESUMO
Public databases contain a planetary collection of nucleic acid sequences, but their systematic exploration has been inhibited by a lack of efficient methods for searching this corpus, which (at the time of writing) exceeds 20 petabases and is growing exponentially1. Here we developed a cloud computing infrastructure, Serratus, to enable ultra-high-throughput sequence alignment at the petabase scale. We searched 5.7 million biologically diverse samples (10.2 petabases) for the hallmark gene RNA-dependent RNA polymerase and identified well over 105 novel RNA viruses, thereby expanding the number of known species by roughly an order of magnitude. We characterized novel viruses related to coronaviruses, hepatitis delta virus and huge phages, respectively, and analysed their environmental reservoirs. To catalyse the ongoing revolution of viral discovery, we established a free and comprehensive database of these data and tools. Expanding the known sequence diversity of viruses can reveal the evolutionary origins of emerging pathogens and improve pathogen surveillance for the anticipation and mitigation of future pandemics.
Assuntos
Computação em Nuvem , Bases de Dados Genéticas , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , Alinhamento de Sequência/métodos , Virologia/métodos , Viroma/genética , Animais , Arquivos , Bacteriófagos/enzimologia , Bacteriófagos/genética , Biodiversidade , Coronavirus/classificação , Coronavirus/enzimologia , Coronavirus/genética , Evolução Molecular , Vírus Delta da Hepatite/enzimologia , Vírus Delta da Hepatite/genética , Humanos , Modelos Moleculares , Vírus de RNA/classificação , Vírus de RNA/enzimologia , RNA Polimerase Dependente de RNA/química , RNA Polimerase Dependente de RNA/genética , SoftwareRESUMO
Bipedal trackways discovered in 1978 at Laetoli site G, Tanzania and dated to 3.66 million years ago are widely accepted as the oldest unequivocal evidence of obligate bipedalism in the human lineage1-3. Another trackway discovered two years earlier at nearby site A was partially excavated and attributed to a hominin, but curious affinities with bears (ursids) marginalized its importance to the paleoanthropological community, and the location of these footprints fell into obscurity3-5. In 2019, we located, excavated and cleaned the site A trackway, producing a digital archive using 3D photogrammetry and laser scanning. Here we compare the footprints at this site with those of American black bears, chimpanzees and humans, and we show that they resemble those of hominins more than ursids. In fact, the narrow step width corroborates the original interpretation of a small, cross-stepping bipedal hominin. However, the inferred foot proportions, gait parameters and 3D morphologies of footprints at site A are readily distinguished from those at site G, indicating that a minimum of two hominin taxa with different feet and gaits coexisted at Laetoli.
Assuntos
Pé/anatomia & histologia , Pé/fisiologia , Fósseis , Marcha/fisiologia , Hominidae/classificação , Hominidae/fisiologia , Animais , Arquivos , Feminino , Hominidae/anatomia & histologia , Humanos , Imageamento Tridimensional , Lasers , Masculino , Modelos Biológicos , Pan troglodytes/anatomia & histologia , Pan troglodytes/fisiologia , Fotogrametria , Filogenia , Tanzânia , Ursidae/anatomia & histologia , Ursidae/fisiologiaRESUMO
Tropospheric ozone (O3) is a key component of air pollution and an important anthropogenic greenhouse gas1. During the twentieth century, the proliferation of the internal combustion engine, rapid industrialization and land-use change led to a global-scale increase in O3 concentrations2,3; however, the magnitude of this increase is uncertain. Atmospheric chemistry models typically predict4-7 an increase in the tropospheric O3 burden of between 25 and 50 per cent since 1900, whereas direct measurements made in the late nineteenth century indicate that surface O3 mixing ratios increased by up to 300 per cent8-10 over that time period. However, the accuracy and diagnostic power of these measurements remains controversial2. Here we use a record of the clumped-isotope composition of molecular oxygen (18O18O in O2) trapped in polar firn and ice from 1590 to 2016 AD, as well as atmospheric chemistry model simulations, to constrain changes in tropospheric O3 concentrations. We find that during the second half of the twentieth century, the proportion of 18O18O in O2 decreased by 0.03 ± 0.02 parts per thousand (95 per cent confidence interval) below its 1590-1958 AD mean, which implies that tropospheric O3 increased by less than 40 per cent during that time. These results corroborate model predictions of global-scale increases in surface pollution and vegetative stress caused by increasing anthropogenic emissions of O3 precursors4,5,11. We also estimate that the radiative forcing of tropospheric O3 since 1850 AD is probably less than +0.4 watts per square metre, consistent with results from recent climate modelling studies12.