RESUMO
Bluetongue (BT), caused by bluetongue virus (BTV), is an arthropod-borne viral disease in ruminants. However, information about BTV infection in yaks in China is limited. Moreover, no such data concerning BTV in Tibetan sheep is available. Therefore, 3771 serum samples were collected from 2187 Tibetan sheep and 1584 yaks between April 2013 and March 2014 from Tibetan Plateau, western China, and tested for BTV antibodies using a commercially available ELISA kit. The overall seroprevalence of BTV was 17.34% (654/3771), with 20.3% (443/2187) in Tibetan sheep and 13.3% (211/1584) in yaks. In the Tibetan sheep group, the seroprevalence of BTV in Luqu, Maqu, Tianzhu, and Nyingchi Prefecture was 20.3%, 20.8%, 20.5%, and 19.1%, respectively. The seroprevalence of BTV in different season groups varied from 16.5% to 23.4%. In the yak group, BTV seroprevalence was 12.6%, 15.5%, and 11.0% in Tianzhu, Maqu, and Luqu counties, respectively. The seroprevalence in different seasons was 12.6%, 15.5%, 15.4%, and 9.0% in spring, summer, autumn, and winter, respectively. The season was the major risk factor concerning BTV infection in yaks (P < 0.05). The date of the BTV seroprevalence in Tibetan sheep and yaks provides baseline information for controlling BT in ruminants in western China.
Assuntos
Vírus Bluetongue/imunologia , Bluetongue/epidemiologia , Bluetongue/etiologia , Ovinos/virologia , Animais , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Bluetongue/sangue , Bluetongue/virologia , Bovinos , China/epidemiologia , Fatores de Risco , Estudos Soroepidemiológicos , Ovinos/sangue , Tibet/epidemiologiaRESUMO
The cellular immune response of cattle to virulent and avirulent (inactivated) bluetongue virus (BTV) was studied. Each of three calves received three vaccinations (sensitizations) with binary ethyleneimine (BEI)-inactivated BTV, 3 weeks apart. The sensitized animals were challenged with BTV-11 strain UC8 3 weeks after the last vaccination. BTV-seropositive and BTV-seronegative calves were used as controls. The animals were bled weekly for virus isolation and for evidence of cell-mediated immunity (CMI) as determined by the lymphocyte stimulation test (LST). Peripheral blood mononuclear leukocytes (PBM L) cultures were induced with purified BTV antigen; the phytomitogens phytohemagglutinin (PHA), Concanavalin A (ConA) and pokeweed (PKW) mitogen, and combinations of phytomitogens and BTV antigen. LST data were analysed by ANOVA and reported as counts per minute (CPM) and stimulation index (SI). Following BTV challenge exposure, significant SI to mitogens were found in PBML cultures for all animals. BTV antigen induced a weak CMI response. There was evidence of perturbations in lymphocyte response as characterised by a sharp decrease in lymphocyte response to mitogens following combined BTV-antigen and mitogen PBML induction. The SI diminished in PBML cultures after a 4 day incubation period, except for ConA. These results provide evidence that the cell-mediated immune response could be affected by BTV and that inhibitory mediators might play an important role in the pathogenesis of BTV in cattle.
Assuntos
Vírus Bluetongue/classificação , Vírus Bluetongue/imunologia , Bluetongue/etiologia , Bluetongue/imunologia , Doenças dos Bovinos/etiologia , Doenças dos Bovinos/imunologia , Imunidade Celular , Animais , Anticorpos Antivirais/biossíntese , Antígenos Virais/administração & dosagem , Vírus Bluetongue/patogenicidade , Bovinos , Tolerância Imunológica , Técnicas In Vitro , Ativação Linfocitária , Linfócitos/imunologia , Linfócitos/virologia , Mitógenos/farmacologia , Testes de Neutralização , Sorotipagem , Vacinação , Vacinas de Produtos Inativados/farmacologia , Vacinas Virais/farmacologiaRESUMO
Strategies designed to minimize the probability of bluetongue virus (BTV) introduction to new areas should be based on a quantitative assessment of the probability of actually establishing the virus once it is introduced. The risk of introducing a new strain of bluetongue virus into a region depends on the number of viremic animals that enter and the competency of local vectors to transmit the virus. We used Monte Carlo simulation to model the probability of introducing BTV into California, USA, and the US through importation of cattle. Records of cattle and calf imports into California and the US were obtained, as was seroprevalence information from the exporting countries. A simulation model was constructed to evaluate the probability of importing either a viremic PCR-negative animal after 14-day quarantine, a c-ELISA BTV-antibody-negative animal after 28-day quarantine, or an untested viremic animal after 100-day quarantine into California and into the US. We found that for animals imported to the US, the simulated (best to worst scenarios) median percentage that tested positive for BTV-antibody ranged from 5.4 to 7.2%, while for the subset imported to California, the simulated median percentage that tested positive for BTV-antibody ranged from 20.9 to 78.9%. Using PCR, for animals imported to the US these values were 71.8-85.3%, and for those imported to California, the simulated median that test positive ranged from 74.3 to 92.4%. The probability that an imported animal was BTV-viremic is very low regardless of the scenario selected (median probability=0.0%). The probability of introducing an exotic strain of BTV into California or the US by importing infected cattle was remote, and the current Office International des Epizooties (OIE) recommendation of either a final PCR test performed 14 days after entry into quarantine, a c-ELISA performed 28 days after entry into quarantine or a 100-day quarantine with no testing requirement was adequate to protect cattle in the US and California from an exotic strain of BTV.
Assuntos
Vírus Bluetongue/imunologia , Bluetongue/epidemiologia , Bluetongue/virologia , Quarentena/veterinária , Animais , Anticorpos Antivirais/análise , Bluetongue/etiologia , Bluetongue/prevenção & controle , Vírus Bluetongue/classificação , Vírus Bluetongue/genética , California/epidemiologia , Bovinos , Ensaio de Imunoadsorção Enzimática/veterinária , Reação em Cadeia da Polimerase/veterinária , Medição de Risco , Estados Unidos/epidemiologiaRESUMO
Fifty-four Merino crossbred sheep were inoculated with bluetongue virus serotype 20 (BTV-20) by the intravenous, subcutaneous and intradermal routes. BTV-20 was successfully transmitted by Culicoides (Avaritia) spp. No. 5 to two additional sheep. Clinical and pathological effects were studied. In the artificially infected sheep, clinical signs were observed after an incubation period of 6 to 10 days and consisted of pyrexia, oral and subcutaneous hyperaemia mild oedema of the ears, face and lips, and coronitis. The major internal pathological changes were petechial and ecchymotic haemorrhages in the tunica media of the pulmonary artery near its junction with the heart and mild haemorrhage and mild oedema in the intestines, coronet, lips, cheeks and ears. Viraemia was detected between day 2 and day 14 post inoculation. The two sheep infected by insect transmission were mildly affected and became viraemic between 16 and 19 days after transmission. No deaths occurred and under experimental conditions BTV-20 caused only mild disease in housed sheep. To date there has been no reported outbreak of natural bluetongue infection in Australia. Compared to other serotypes BTV-20 appears to be of low pathogenicity in sheep.
Assuntos
Bluetongue/patologia , Animais , Anticorpos Antivirais/análise , Bluetongue/etiologia , Vírus Bluetongue/imunologia , Ceratopogonidae/microbiologia , Febre/veterinária , Linfopenia/veterinária , Boca/patologia , Músculos/patologia , Nariz/patologia , OvinosRESUMO
Bluetongue (BT) is a viral disease of domestic and wild ruminants. It is particularly damaging in sheep, where up to half of infected animals may die, showing inflammation and hemorrhages of the mucous membranes of the mouth, nose, and intestines. In cattle and goats, BT rarely causes disease, however it can affect the animal's reproductive ability, so that losses are not easily estimated. Bluetongue virus spreads from animal to animal by biting insects of the genus Culicoides; and this is the reason why the disease is more prevalent in geographic areas where climate conditions are favourable for their development. The disease was first recognized in South Africa in the late 1700's, but it was not until the early 1900's that it was described in detail, and at present, epizootiology and pathogenesis studies are still being carried on.
Assuntos
Vírus Bluetongue , Bluetongue , Aborto Animal/etiologia , Aborto Animal/virologia , Animais , Antígenos Virais/imunologia , Argentina/epidemiologia , Bluetongue/diagnóstico , Bluetongue/etiologia , Bluetongue/história , Bluetongue/prevenção & controle , Vírus Bluetongue/classificação , Vírus Bluetongue/isolamento & purificação , Vírus Bluetongue/fisiologia , Ceratopogonidae/virologia , Feminino , Doenças Fetais/veterinária , Doenças Fetais/virologia , História do Século XVII , História do Século XIX , História do Século XX , Infertilidade Masculina/veterinária , Infertilidade Masculina/virologia , Insetos Vetores , Masculino , RNA Viral/análise , Ruminantes , Proteínas Virais/imunologia , Vacinas ViraisRESUMO
Objetivou-se com este estudo determinar os aspectos epidemiológicos da infecção pelo Vírus da Língua Azul (VLA) em bovinos leiteiros na microrregião de Garanhuns, Estado de Pernambuco, Brasil. Foram coletadas 384 amostras de soro de bovinos fêmeas em idade reprodutiva, procedentes de 20 propriedades dos 19 municípios que compõem a região. As amostras foram testadas com a prova de imunodifusão em gel de agarose (IDGA) para pesquisa de anticorpos anti-VLA. Observou-se ocorrência de 71,3% (274/384; IC 95% - 66,5% - 75,7%) de animais positivos. Em 100% das propriedades houve ao menos um animal soropositivo. Os fatores de risco identificados foram: presença de áreas alagadas (OR=11,8; p=0,001), não realizar controle de insetos (OR=2,1; p=0,033), rebanho aberto (OR=2,1; p=0,001) e utilização de inseminação artificial (OR=8,8; p=0,003). Este é o primeiro registro de detecção de anticorpos anti-VLA em bovinos no Estado de Pernambuco. Conclui-se que a infecção pelo VLA ocorre em bovinos na área estudada e sugere-se que medidas de controle baseadas no manejo higiênico-sanitário e biosseguridade sejam implantadas para evitar a propagação do vírus, tais como: eliminação de áreas alagadiças; controle de insetos; utilizar sêmen na inseminação artificial com atestado sanitário; realizar exames sorológicos ao adquirir animais.(AU)
The objective of this study was to determine epidemiological aspects of Bluetongue Virus (BTV) infection on dairy cattle in the Garanhuns microregion, Pernambuco state, Brazil. Three hundred eighty-four (384) serum samples of female bovines of reproductive age were collected from 20 farms of the 19 municipalities that make up the region. Samples were tested with the agarose gel immunodiffusion test (AGID) for anti-VLA antibody screening. There were 71.3% (274/384, 95% CI - 66.5% - 75.7%) positive animals. In 100% of the farms there was at least one seropositive animal. The risk factors identified were: presence of flooded areas (OR=11.8, p=0.001), absence of insect control (OR=2.1, p=0.033), open herd (OR=2.1; p=0.001) and use of artificial insemination (OR=8.8, p=0.003). This is the first record of detection of anti-BTV antibodies in cattle in Pernambuco state. It is concluded that BTV infection occurs in cattle in the studied area, and it is suggested that control measures based on hygienic-sanitary management and biosecurity are in place to prevent the spread of the virus, such as elimination of wetlands; Insect control; semen used in artificial insemination with health certificate; Serological tests when acquiring animals.(AU)
Assuntos
Animais , Bovinos , Vírus Bluetongue , Bluetongue/epidemiologia , Bluetongue/etiologia , Fatores de Risco , Brasil/epidemiologia , Imunodifusão/veterináriaAssuntos
Vírus Bluetongue/classificação , Bluetongue/epidemiologia , Bluetongue/virologia , Animais , Anticorpos Antivirais/sangue , Bluetongue/etiologia , Bluetongue/patologia , DNA Viral/análise , Ensaio de Imunoadsorção Enzimática/veterinária , Cabras , Países Baixos/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Sorotipagem , OvinosAssuntos
Vírus Bluetongue/patogenicidade , Bluetongue/epidemiologia , Bluetongue/transmissão , Surtos de Doenças/veterinária , Transmissão de Doença Infecciosa/veterinária , Animais , Bluetongue/sangue , Bluetongue/etiologia , Bluetongue/virologia , Europa (Continente)/epidemiologia , Estações do Ano , OvinosAssuntos
Vírus Bluetongue/isolamento & purificação , Bluetongue/epidemiologia , Doenças das Cabras/epidemiologia , Animais , Anticorpos Antivirais/sangue , Bluetongue/etiologia , DNA Viral/análise , Ensaio de Imunoadsorção Enzimática/veterinária , Doenças das Cabras/etiologia , Cabras , Sorotipagem/veterinária , Taiwan/epidemiologiaRESUMO
Bluetongue virus (BTV) is the cause of bluetongue, an emerging, arthropod-transmitted disease of ungulates. Bluetongue is characterized by vascular injury with hemorrhage, tissue infarction and widespread edema, lesions that are consistent with those of the so-called viral hemorrhagic fevers. To further investigate the pathogenesis of vascular injury in bluetongue, we utilized an electrical impedance assay and immunofluorescence staining to compare the effects of BTV infection on cultured bovine endothelial cells (bPAEC) with those of inducers of cell death (Triton X-100) and interendothelial gap formation (tissue necrosis factor [TNF]). The data confirm that the adherens junctions of BTV-infected bPAECs remained intact until 24h post-infection, and that loss of monolayer impedance precisely coincided with onset of virus-induced cell death. In contrast, recombinant bovine TNF-alpha caused rapid loss of bPAEC monolayer impedance that was associated with interendothelial gap formation and redistribution of VE-cadherin, but without early cell death. The data from these in vitro studies are consistent with a pathogenesis of bluetongue that involves virus-induced vascular injury leading to thrombosis, hemorrhage and tissue necrosis. However, the contribution of cytokine-induced interendothelial gap formation with subsequent edema and hypovolemic shock contributes to the pathogenesis of bluetongue remains to be fully characterized.
Assuntos
Vírus Bluetongue/patogenicidade , Bluetongue/patologia , Bluetongue/fisiopatologia , Junções Aderentes/patologia , Animais , Bluetongue/etiologia , Bovinos , Morte Celular/efeitos dos fármacos , Morte Celular/fisiologia , Células Cultivadas , Impedância Elétrica , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/patologia , Células Endoteliais/fisiologia , Células Endoteliais/virologia , Octoxinol/farmacologia , Proteínas Recombinantes/farmacologia , Fator de Necrose Tumoral alfa/farmacologia , Replicação ViralAssuntos
Insetos , Animais , Bluetongue/etiologia , Colorado , Feminino , Larva , Masculino , Estações do Ano , Razão de Masculinidade , OvinosAssuntos
Ceratopogonidae , Animais , Artiodáctilos , Bluetongue/etiologia , Bovinos , Comportamento Alimentar , Feminino , Cabras , Cavalos , Insetos Vetores , Israel , Mamíferos , Testes de Precipitina , Coelhos , Ovinos , Doenças dos Ovinos/etiologia , Vírus/isolamento & purificaçãoAssuntos
Anticorpos Antivirais/análise , Bluetongue/imunologia , Testes de Hemaglutinação , Reoviridae/imunologia , Animais , Antígenos Virais/isolamento & purificação , Bluetongue/etiologia , Bluetongue/microbiologia , Centrifugação com Gradiente de Concentração , Eritrócitos/imunologia , Testes de Inibição da Hemaglutinação , Cavalos/imunologia , Imunodifusão , Injeções Subcutâneas , Reoviridae/isolamento & purificação , Ovinos , TaninosAssuntos
Cabras , Vírus de RNA/isolamento & purificação , Doenças dos Ovinos/microbiologia , Viroses/veterinária , Animais , Bluetongue/sangue , Bluetongue/etiologia , Bluetongue/imunologia , Bluetongue/microbiologia , Temperatura Corporal , Embrião de Galinha , Feminino , Hematócrito , Imunodifusão , Contagem de Leucócitos , Masculino , Ovinos , Cultura de VírusAssuntos
Vírus Bluetongue/imunologia , Bluetongue/etiologia , Doenças do Cão/etiologia , Complicações Infecciosas na Gravidez/veterinária , Vacinas Virais/efeitos adversos , Animais , Cães , Contaminação de Medicamentos , Feminino , Gravidez , Complicações Infecciosas na Gravidez/etiologia , Vacinas Virais/normasAssuntos
Aborto Animal/etiologia , Bluetongue/etiologia , Doenças do Cão/etiologia , Complicações Infecciosas na Gravidez/veterinária , Vacinas Virais/efeitos adversos , Animais , Bluetongue/mortalidade , Vírus Bluetongue/isolamento & purificação , Doenças do Cão/mortalidade , Cães , Combinação de Medicamentos , Contaminação de Medicamentos , Feminino , Imunização Secundária/veterinária , Placenta/microbiologia , Gravidez , Complicações Infecciosas na Gravidez/etiologia , Complicações Infecciosas na Gravidez/mortalidade , Vacinação/efeitos adversos , Vacinação/veterináriaAssuntos
Anencefalia/veterinária , Doenças dos Bovinos/etiologia , Doenças dos Ovinos/etiologia , Viroses/veterinária , Animais , Anticorpos/análise , Bluetongue/etiologia , Bovinos , Doenças dos Bovinos/congênito , Doenças dos Bovinos/imunologia , Embrião de Galinha , Colostro , Técnicas de Cultura , Feminino , Hidranencefalia/etiologia , Hidranencefalia/imunologia , Hidranencefalia/veterinária , Gravidez , Ovinos , Cultura de Vírus , Viroses/congênito , Viroses/imunologiaRESUMO
In August 2000 bluetongue (BT) disease appeared amongst sheep on the island of Sardinia spreading later to Sicily and to mainland Italy. The majority of areas affected by BT were surveyed for Culicoides imicola, the only proven vector of the disease known to occur in the Mediterranean region. The data from 1456 light-trap collections, made in months with a mean temperature of 12.5 degrees C, were used to test the accuracy of current models predicting the prevalence and abundance of C. imicola across the region. For Italy, the distribution of C. imicola was found to be very irregular and did not fit the modelled predictions. The possible reasons for this are discussed, and suggestions made as to which variables may improve this fit in the development of future risk models. In Italy, past surveys failed to reveal the presence of C. imicola, and so could be construed as evidence of its recent invasion, and thus rampant spread northwards. Although equivocal, historical records indicate that C. imicola was overlooked in the past. Six recommendations are made as to the possible future course of Culicoides research in southern Europe.