RESUMO
We have previously identified a molecule (named cell adhesion molecule [CAM]) that is involved in the in vitro aggregation of neural cells from chick embryos. In the present report, specific anti-CAM antibodies have been used to demonstrated that CAM is localized in neural tissues, and is associated with the plasma membrane of retinal cells and neurites. Furthermore, it has been shown by antibody absorption techniques that the decreased adhesiveness of cultured retinal cells obtained originally from older embryos is correlated with a decrease in the density or accessibility of cell adhesion molecules on the surface of these cells. The central role of CAM in neural cell aggregation has been established by the observation that anti-CAM Fab' fragments inhibit adhesion between neural cells in a variety of assays. To investigate the function of CAM and cell adhesion in developing tissues, aggregates of retinal cells that are capable of forming histotypic patterns in vitro were cultured in the presence and absence of anti-CAM Fab'. The Fab' was found to inhibit sorting out of cell bodies and neurites and to decrease the number of membrane-membrane contacts, suggesting that CAM is associated with cell-cell, cell-neurite, and neurite-neurite interactions.
Assuntos
Adesão Celular , Retina/citologia , Animais , Agregação Celular , Diferenciação Celular , Membrana Celular/análise , Células Cultivadas , Embrião de Galinha , Gânglios/análise , Nervo Óptico/análise , Retina/análise , Medula Espinal/análiseRESUMO
Octopamine has been identified and measured in individual neurons from Aplysia californica. Neither dopamine nor norepinephrine was detected in these cells. Thus, in Aplysia there may be separate populations of catecholaminergic and monophenolaminergic cells. Octopamine may have functions of its own in the central nervous system of mollusks.
Assuntos
Moluscos/análise , Neurônios/análise , Neurotransmissores , Octopamina/análise , Transmissão Sináptica , Animais , Separação Celular , Dopamina/análise , Gânglios/análise , Neurônios/fisiologia , Norepinefrina/análise , Octopamina/fisiologiaRESUMO
An adenylate cyclase that is activated specifically by very low concentrations of octopamine has been identified both in homogenates and in intact cells of the thoracic ganglia of an insect nervous system. This enzyme appears to be distinct from two other adenylate cyclases present in the same tissue, which are activated by dopamine and by 5-hydroxytryptamine, respectively. The data raise the possibility of a role of octopamine-sensitive adenylate cyclase in the physiology of synaptic transmission.
Assuntos
Adenilil Ciclases/biossíntese , Gânglios/enzimologia , Octopamina/farmacologia , Animais , Baratas , AMP Cíclico/análise , AMP Cíclico/metabolismo , Dopamina/farmacologia , Ativação Enzimática , Gânglios/análise , Octopamina/antagonistas & inibidores , Octopamina/fisiologia , Fentolamina/farmacologia , Propranolol/farmacologia , Serotonina/farmacologia , Transmissão SinápticaRESUMO
A cardioexcitatory substance from ganglia of the clam Macrocallista nimbosa, formerly designated peak C, is the tetrapeptide amide Phe-Met-Arg-Phe-NH2. Its structure was determined by the combined use of Edman dansyl degradation and tryptic digestion. The structure was confirmed by synthesis. This neuropeptide is active at about 10(-8)M when assayed on molluscan muscle.
Assuntos
Bivalves/análise , Coração/efeitos dos fármacos , Proteínas do Tecido Nervoso , Oligopeptídeos , Sequência de Aminoácidos , Animais , Gânglios/análise , Contração Muscular/efeitos dos fármacos , Proteínas do Tecido Nervoso/isolamento & purificação , Proteínas do Tecido Nervoso/farmacologia , Oligopeptídeos/isolamento & purificação , Oligopeptídeos/farmacologia , Relação Estrutura-AtividadeRESUMO
Individual neurons can be recognized and identified anatomically, physiologically, and biochemically in the insect central nervous system. Biochemical analyses of extracts prepared from one such identified neuron show it to be associated with a bioactive pentapeptide called proctolin. This peptide may be a neurotransmitter, and a preparation is established in which its physiological action can be studied at the cellular level.
Assuntos
Neurônios/análise , Neuropeptídeos , Neurotransmissores/análise , Oligopeptídeos/análise , Animais , Bioensaio , Baratas , Gânglios/análise , Gafanhotos , Contração Muscular/efeitos dos fármacos , Neurotransmissores/farmacologia , Oligopeptídeos/farmacologiaRESUMO
Comparable depression of synaptic transmission in the avian ciliary ganglion resulted from either section or localized colchicine treatment of the ciliary nerves. Both colchicine treatment and axotomny produced similar changes in RNA distribution in the cell bodies as well. Colchicine did not directly affect transmission, and action potential propagation along the ciliary nerves was normal. Interference with axoplasmic transport of material in both cases is postulated to signal the observed chromatolytic changes.
Assuntos
Axônios/efeitos dos fármacos , Colchicina/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Potenciais de Ação , Animais , Transporte Axonal/efeitos dos fármacos , Galinhas , Corpo Ciliar/inervação , Estimulação Elétrica , Potenciais Evocados , Gânglios/análise , Gânglios/citologia , Gânglios/cirurgia , RNA/análiseRESUMO
Spectral analysis at liquid N2 temperature of the circumesophageal ganglia of Aplysia juliana showed that carotenoids and a hemoglobin-like pigment are contained in concentrations of approx. 25 and 3 micronM, respectively, in the whole ganglia. Microspectrophotometrical measurements of Aplysia neurons indicated that the carotenoids reside on lipochondria in a concentration of approx. 38 mM. In addition to lipochondria, two types of pigmented particulate having absorption maxima at about 512 and 525 nm, respectively, were found in the neurons. The neuronal carotenoids consist of violaxanthin, beta-carotene and one minor component; among them the first occupies approx. 77% of total carotenoids. Two principal absorption maxima of the carotenoids, when existing in both ganglial homogenates and Triton X-100 extracts, show a red shift of 10 nm compared with those of free pigments in hexan. The red shift may be interpreted as due to the solvation of the carotenoids by surrounding lipids.
Assuntos
Aplysia/análise , Carotenoides , Neurônios/análise , Pigmentos Biológicos , Animais , Carotenoides/isolamento & purificação , Gânglios/análise , Pigmentos Biológicos/isolamento & purificação , Polietilenoglicóis , EspectrofotometriaRESUMO
Peptide histidine isoleucine amide (PHI) and vasoactive intestinal polypeptide (VIP) are fragments of the same precursor molecule, prepro-VIP, and coexistence of the two peptides is, therefore, to be expected. Nerve fibers displaying PHI and VIP immunoreactivity occurred around blood vessels and follicles in the thyroid gland of several species. Sequential staining with antibodies against PHI and VIP revealed coexistence of the two peptides in the same population of nerve cell bodies in ganglia situated along the laryngeal nerves and in intrathyroid nerve fibers. Chemical sympathectomy (6-hydroxydopamine treatment), surgical sympathectomy (removal of the superior cervical ganglia), and unilateral cervical vagotomy (removal of the nodose ganglion) failed to affect the number and distribution of PHI/VIP fibers in the thyroid gland. Taken together, the findings suggest that both the perivascular and interfollicular PHI/VIP fibers originate in laryngeal ganglia. PHI weakly stimulated basal thyroid hormone secretion in mice in vivo, but did not influence the response to TSH or VIP. PHI had no effect on calcitonin secretion in rats. Like VIP, PHI may play a physiological role in the regulation of thyroid hormone secretion.
Assuntos
Nervos Laríngeos/análise , Fibras Nervosas/análise , Peptídeos/análise , Glândula Tireoide/inervação , Hormônios Tireóideos/metabolismo , Peptídeo Intestinal Vasoativo/análise , Animais , Calcitonina/metabolismo , Gatos , Bovinos , Galinhas , Cães , Imunofluorescência , Gânglios/análise , Cobaias , Histocitoquímica , Camundongos , Peptídeo PHI , Peptídeos/farmacologia , Ratos , Ovinos , Glândula Tireoide/efeitos dos fármacos , Glândula Tireoide/metabolismo , Distribuição TecidualRESUMO
The distribution of substance P-like immunoreactivity (substance P-li) in the central nervous system of Limulus polyphemus was studied by using indirect immunocytochemical techniques. Six bilaterally symmetrical pairs of cell clusters in the circumesophageal connectives and the subesophageal mass contain substance P-li. Two of those pairs are the source of a system of efferent fibers that is involved in the expression of circadian rhythms of photosensitivity by the lateral eye. Substance P-li-containing cells were also observed scattered along the length of the circumesophageal connectives, which contain abundant stained fibers and some terminals. Substance P-li fibers leave through the ventral and dorsal nerves of the posterior circumesophageal ring. The neuropil of the subesophageal mass contains an abundance of stained terminals. Immunoreactive fibers can be seen throughout the length of the two longitudinal connectives of the ventral cord, in discrete fiber tracts in the lateral edges of the interganglionic connectives, and in the dorsal and ventral nerves of abdominal ganglia 1-4. Each of these ganglia contains three pairs of substance P-immunoreactive cell body clusters: an anterolateral, a medial longitudinal, and a medial posterior cluster. Substance P-li fibers entering through the ventral (posterior) nerves form very distinctive fascicles in each side of the ganglia, giving off fibers throughout their length. The neuropil is filled with immunoreactive terminals distributed homogeneously. The anterolateral clusters of the abdominal ganglia may be involved in cardioregulation. The six pairs of clusters in the posterior circumesophageal ring, and perhaps some of those in the abdominal ganglia, are believed to constitute a neurosecretory system, projecting to multiple targets throughout the organism. This system is postulated to modulate various sensory inputs and motor activity, and could be driven by a circadian clock, as well as by other systems responsible for integrated organismic responses.
Assuntos
Autacoides/análise , Sistema Nervoso Central/análise , Caranguejos Ferradura/análise , Peptídeos/análise , Animais , Autacoides/fisiologia , Gânglios/análise , Imunoquímica , Peptídeos e Proteínas de Sinalização Intercelular , Peptídeos/fisiologiaRESUMO
The distribution of proctolin-like immunoreactive (PLI) cells was mapped in the central nervous system of the leech Hirudo medicinalis. In segmental ganglia, PLI cells can be divided into two groups: cells that stain repeatedly in every successive ganglion, and cells that stain only in specific segmental ganglia. The number of PLI cells, therefore, ranged from eight to 20 cells per ganglion. One bilateral pair of PLI cells (cells PLI-1) was further characterized morphologically by Lucifer yellow and horseradish peroxidase cell injections. Cell PLI-1 conforms in soma position, morphology, and physiological properties with cell 101, which has been previously classified as an inhibitory motoneuron to the flattener muscles. A locust bioassay (O'Shea and Adams, Science 213:567-569, 1981) was used to detect the presence of proctolin-like bioactivity. Extracts of leech ganglia when applied to the extensor tibialis muscle of the locust leg induced a proctolin-like response similar to the responses induced by proctolin standards. This work extends the finding of proctolin-like substances to the annelid phylum.
Assuntos
Gânglios/metabolismo , Sanguessugas/metabolismo , Neuropeptídeos , Oligopeptídeos/metabolismo , Animais , Bioensaio , Imunofluorescência , Gânglios/análise , Gânglios/citologia , Oligopeptídeos/análiseRESUMO
Neuron L11 in the abdominal ganglion of Aplysia californica is thought to be both cholinergic and peptidergic. In previous studies, we isolated a cDNA clone encoding the precursor for an L11 secreted protein(s) by differentially screening an abdominal ganglion cDNA library. We now report the isolation of genomic clones encoding the L11 cDNA sequences. Analysis of these clones reveals that the gene is present in a single copy per haploid genome. RNA blotting and cDNA cloning demonstrate that the L11 gene is expressed not only in the abdominal ganglion but in the head ganglia as well. To define the positions of cells expressing this gene and to follow their processes, we raised antibodies to synthetic peptides defined by the cDNA sequence. Histochemistry revealed about 100 neurons containing immunoreactive material. These cells arborize in the neuropil and are distributed throughout the central nervous system, representing about 0.5% of the Aplysia central neurons. In addition, cells in the abdominal ganglion send processes to the mantle floor at the base of the gill via the genital and branchial nerves. Our data suggest that this network of cells expresses the single L11 peptide gene.
Assuntos
Aplysia/genética , Sistema Nervoso Central/análise , Genes , Proteínas do Tecido Nervoso/genética , Animais , Aplysia/análise , Clonagem Molecular , DNA/genética , Gânglios/análise , Técnicas Imunológicas , Proteínas do Tecido Nervoso/análise , Neurônios/análiseRESUMO
Somatostatin (SRIF) is a neuropeptide with a widespread distribution in the mammalian CNS. In the present study we have examined the distribution of immunoreactive-like SRIF (ISRIF)-containing elements in the nervous system of the cephalopod mollusk Loligo pealei, or the Woods Hole squid. ISRIF was localized by light immunocytochemistry in sections of the squid-optic lobe, circumesophageal ganglia-and in stellate ganglion. In the optic lobe, ISRIF neurons were found in the internal granule cell layer and medulla and immunoreactive fibers were seen throughout the lobe and in the optic tract but were absent from the optic nerve, i.e., the projection between the retina and optic lobe. In the supraesophageal complex, ISRIF neurons were found in all lobes, but primarily in the vertical, subvertical, and frontal. In the subesophageal ganglion, ISRIF neurons were seen mainly following unilateral pallial nerve lesions; these neurons were primarily small-to-medium sized. ISRIF fibers were seen in many of the nerves exiting from the brain and in nerves extending between the sub- and supra-esophageal ganglia. In the stellate ganglion, ISRIF was present in many neurons as well as in a plexus of fibers within the ganglion; the peptide was absent from the second-order fibers and the giant axon. The data suggest that a molecule immunologically similar to vertebrate SRIF may be a major transmitter/modulator in this invertebrate. These results provide a foundation for further studies to evaluate the role of this molecule.
Assuntos
Peptídeos/análise , Somatostatina/análise , Animais , Química Encefálica , Decapodiformes , Esôfago/inervação , Gânglios/análise , Técnicas Imunoenzimáticas , Sistema Nervoso/análise , Retina/análise , Vias Visuais/análiseRESUMO
The distribution of GABA-like immunoreactivity in the brain of the honeybee was investigated with antisera generated against GABA protein conjugates. The binding of the antisera in paraffin serial sections was studied with the peroxidase-antiperoxidase method. GABA-like immunoreactive fibers appeared in all main neuropile areas. The staining of the optic lobes showed pronounced stratification. The receptor cells of compound eyes, ocelli, and antennae were not labelled. Several prominent fiber tracts showed GABA-like immunoreactivity, whereas other tracts were devoid of staining. There are no major immunoreactive commissures linking the two brain hemispheres with the exception of small commissures that bridge short distances between the beta-lobes and the antennal lobes. Several fibers in the cervical connective were also labelled; some of those may descend from the suboesophageal ganglion to the thoracic ganglia. The dense reactivity seen in the optic and antennal neuropiles implies that GABA is more important in mediating local rather than more distant neural interactions.
Assuntos
Ácido gama-Aminobutírico/análise , Animais , Abelhas , Química Encefálica , Mapeamento Encefálico , Esôfago/inervação , Gânglios/análise , Soros Imunes , Órgãos dos Sentidos/análise , Vias Visuais/análise , Ácido gama-Aminobutírico/imunologiaRESUMO
Retrograde-tracing and immunohistochemical techniques were used in combination to investigate the types of putative transmitters in pelvic neurons that project to the bladder, colon or penis of rats. In addition, populations of axon varicosities associated with these neurons were characterized. Subpopulations of neurons in colchicine-treated major pelvic ganglia and accessory ganglia of male rats contained immunoreactivity (IR) for tyrosine hydroxylase (TH), vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), or enkephalin (ENK), while types of immunoreactivity found in major groups of varicose axons were ENK, cholecystokinin (CCK), and somatostatin (SOM). Substance P (SP)-IR varicose axons were much less common. Bladder and colon neurons were similar in a number of ways. Many neurons contained NPY-IR (greater than or equal to 50%), fewer contained TH-IR (25-30%), and even fewer contained ENK-IR (5-15%) or VIP-IR (5-10%); many neurons were associated with baskets of ENK-IR varicosities (50-65%) and fewer neurons were surrounded by CCK- or SOM-IR varicosities (30-35%). Colon neurons differed from penis neurons in having a slightly larger proportion that contained ENK-IR (10-15%, compared with 1-3%). Penis neurons were markedly different from the other two groups in additional ways. More than 90% of them contained VIP-IR, whereas only 5-7% contained NPY-IR and none were immunoreactive for TH. Furthermore, although the proportion of penile neurons associated with many ENK-IR varicosities was similar to the bladder and colon neurons (45-50%), they were rarely seen close to CCK- or SOM-IR varicose axons. These studies describe similarities and differences in the histochemical properties of neurons which project to the bladder, colon, or penis and of the varicose axons associated with those neurons. This gives further insights into the possible transmitter mechanisms involved in the regulation of different pelvic functions.
Assuntos
Colo/inervação , Neurônios/análise , Pelve/inervação , Pênis/inervação , Ratos Endogâmicos/metabolismo , Bexiga Urinária/inervação , Animais , Gânglios/análise , Imuno-Histoquímica , Masculino , Vias Neurais/análise , Neuropeptídeos/análise , Especificidade de Órgãos , Ratos , Ratos Endogâmicos/anatomia & histologia , Tirosina 3-Mono-Oxigenase/análiseRESUMO
Egg-laying hormone and alpha-bag cell peptide are two neuropeptides derived from a common precursor protein in the marine mollusk Aplysia. Previous studies indicate that they are neurotransmitters that co-exist in individual bag cell neurons and most bag cell processes in the abdominal ganglion. In the present investigation we used double-label immunocytochemistry with highly specific antisera to describe their distribution elsewhere in the CNS. We found that a small cluster of cells and their fibers in the pleural ganglion that were previously described as being immunoreactive for egg-laying hormone were also immunoreactive for alpha-bag cell peptide(1-9). A previously described group of small cell bodies in the cerebral ganglion also stained for both peptides. However, the fiber arborizations located near them were immunoreactive for alpha-bag cell peptide(1-9), but not egg-laying hormone. This suggests that there is alternative processing of the precursor protein or differential transport of the peptides from the cell bodies. The specificities of the antibodies indicate that all of the neurons that stain for egg-laying hormone-like peptides in the CNS synthesize peptides derived from the egg-laying hormone/bag cell peptide precursor, rather than peptides derived from other members of the egg-laying hormone gene family. They also suggest that peptides derived from the related A or B precursor proteins are not synthesized in the CNS, or at levels too low to detect. The results are consistent with the proposal that the behavior associated with egg-laying is initiated and controlled by peptide transmitters derived from a single gene and expressed in specific neurons of the CNS.
Assuntos
Aplysia/metabolismo , Gânglios/análise , Hormônios de Invertebrado/análise , Fragmentos de Peptídeos/análise , Animais , Gânglios/citologiaRESUMO
Serotonin-containing neurons were localized in the central nervous system of Aplysia californica by the combination of an immunohistochemical procedure for wholemounts of Aplysia ganglia and, in parallel experiments, by the direct measurement of serotonin in individual neurons with a radioenzymatic assay. Paraformaldehyde fixed, desheathed ganglia were incubated in a commercially available antiserum against a conjugate of serotonin and bovine serum albumin. The bound antibody was visualized by an indirect antibody procedure using the horseradish peroxidase catalyzed reduction of the chromogen, diaminobenzidine, and a cobalt-nickel intensification procedure. The specificity of the immunoreaction for serotonin-containing cells was demonstrated by (1) the consistent staining of previously identified serotonin-containing neurons; (2) the absence of staining in identifiable neurons which do not contain measurable serotonin; and (3) the absence of staining in ganglia treated with antiserum which had been absorbed by serotonin and the serotonin conjugate. Previously unidentified serotonin-containing neurons were localized in the cerebral and pedal ganglia by the combination of immunocytochemistry and direct assay for serotonin. Immunoreactive fibers were found surrounding many neuronal somata. In addition, serotonin assays of known cholinergic neurons that were covered by immunoreactive fibers indicated that measurable amounts of serotonin were associated with such neurons, but the concentration of serotonin was an order of magnitude lower than in neurons known to stain with the anti-serotonin serum. These studies have localized more than a hundred neurons that appear to contain serotonin in concentrations (greater than 0.5 mM) in the Aplysia central nervous system. In addition, it appears that the long-held belief that the somata of invertebrate neurons are relatively free of impinging nerve fibers may no longer be tenable. The immunoreactive fibers surrounding many cell bodies may be the source of measurable serotonin associated with neurons known to utilize or to contain transmitter substances other than serotonin. Immunocytochemical techniques applied to wholemounts of molluscan preparations facilitate identification of stained neurons for parallel physiological and chemical experiments.
Assuntos
Neurônios/análise , Serotonina/análise , Animais , Aplysia , Gânglios/análise , Gânglios/citologia , Histocitoquímica , Imunoquímica , Fibras Nervosas/análise , Distribuição TecidualRESUMO
Antibodies raised against GABA and glutamate were used to stain sections through locust thoracic ganglia for light and electron microscopy. Using a peroxidase-antiperoxidase method for light microscopy, the GABA antibody was shown to label inhibitory motor neurons thought to use GABA as their neurotransmitter, and the glutamate antibody to label excitatory motor neurons thought to use glutamate. An immunogold method was used to reveal labelled neuropilar processes in the electron microscope. Each antibody specifically labels a particular population of processes. With the GABA antibody, labelling is equally clear whether the processes concerned contain synaptic vesicles or not and is strongly contrasted against very low background levels. With the glutamate antibody, most processes show some affinity for the antibody, probably reflecting the presence of metabolic glutamate, however one population can be clearly distinguished by the presence of a much greater density of gold particles over synaptic vesicles. In the locust it appears, therefore, that the antibody can distinguish clearly between the metabolic and neurotransmitter pools of glutamate. It has been proposed that synaptic vesicles in GABAergic neurons have a different shape to those in glutamatergic neurons. This was supported by the electron microscope immunocytochemistry. Those showing GABA-like immunoreactivity contain predominantly pleomorphic agranular vesicles approximately 21 x 30 nm in diameter. Those showing glutamate-like immunoreactivity contain round agranular vesicles of about 38 nm in diameter. The GABA antibody appears to label all processes containing pleomorphic agranular vesicles. By contrast, some processes containing round agranular vesicles are not labelled by the glutamate antibody, even though the vesicles they contain are statistically identical in size to those in labelled profiles. With neither antibody was the labelling of glial cells greater than the background level.
Assuntos
Glutamatos/análise , Gafanhotos/anatomia & histologia , Neurônios/análise , Vesículas Sinápticas/ultraestrutura , Ácido gama-Aminobutírico/análise , Animais , Especificidade de Anticorpos , Gânglios/análise , Gânglios/ultraestrutura , Glutamatos/metabolismo , Ácido Glutâmico , Gafanhotos/metabolismo , Imuno-Histoquímica , Microscopia Eletrônica , Neurônios/ultraestrutura , Ácido gama-Aminobutírico/metabolismoRESUMO
Dynorphin B(20-32) was visualized by immunohistochemistry in guinea-pig and rat perivascular nerve fibers and was measured by radioimmunoassay within the walls of feline, canine, bovine and human cephalic and systemic arteries and veins. Canine vessels contained the highest levels. When human blood vessels or trigeminal ganglia were subjected to reverse-phase high-performance liquid chromatography, dynorphin immunoreactivity exhibited a retention time identical to that of synthetic dynorphin B. No differences in dynorphin-like immunoreactivity were measurable between feline systemic arteries and veins, or between cephalic and systemic vessels. The highest amounts were present in leptomeninges devoid of large pial arteries. Relatively high levels were also measured in feline and human trigeminal ganglia and feline superior cervical and sphenopalatine ganglia, three sources of projecting perivascular axons. Levels did not diminish, however, in ipsilateral feline cephalic vessels following either unilateral trigeminal or superior cervical ganglionectomies. Hence, dynorphin-containing fibers may project from parasympathetic cell bodies or perhaps from intrinsic brain sources. Previously published reports indicate that the kappa agonist dynorphin does not modify vessel tone when added in vitro but does inhibit release of neurotransmitters from afferent and sympathetic axons via prejunctional receptors. These observations suggest a pharmacological role for dynorphin on sensory and autonomic functions of the vasculature.
Assuntos
Vasos Sanguíneos/inervação , Encéfalo/irrigação sanguínea , Dinorfinas/análise , Fibras Nervosas/análise , Idoso , Idoso de 80 Anos ou mais , Animais , Vasos Sanguíneos/análise , Gatos , Circulação Cerebrovascular , Dinorfinas/metabolismo , Feminino , Gânglios/análise , Gânglios/fisiologia , Cobaias , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Radioimunoensaio , Ratos , Ratos Endogâmicos , Nervo Trigêmeo/análise , Nervo Trigêmeo/fisiologiaRESUMO
Egg-laying hormone in Aplysia californica is synthesized and secreted by cells that seem to be homogeneous ultrastructurally and electrophysiologically. Several conventional methods have been used to demonstrate histochemical homogeneity and special staining techniques based on the known properties of the hormone show the neuroendocrine organ to be uniform in appearance. Furthermore, since stain specificity for egg-laying hormone is demonstrable using release and biochemical studies, the authors concluded that the organ consists of a population of biochemically homogeneous neurons.
Assuntos
Aplysia/citologia , Gânglios/citologia , Gânglios/análise , Hormônios de Invertebrado/análise , Peso Molecular , Coloração e RotulagemRESUMO
We studied the distribution of sugar residues in the oligosaccharide chains of complex carbohydrates in tissue sections of rat spinal cord, brainstem, and sensory ganglia using twelve lectin-horseradish peroxidase conjugates. Glycoconjugates containing terminal galactose residues were localized apparently in the Golgi apparatus in a population of predominantly small B-type neurons in spinal and trigeminal ganglia. Large A-type neurons rarely showed reactivity with galactose-binding lectins. A cells stained for glycoconjugates with N-glycosidically linked oligosaccharides and glycogen. The central and peripheral processes of the small neurons, mostly unmyelinated C fibers in sensory roots and spinal nerves, contained an abundance of glycoconjugates with terminal alpha-galactose residues. The central projections and terminals of small to medium-sized primary sensory neurons in the spinal and trigeminal ganglia were visualized in Lissauer's tract and the substantia gelatinosa in the spinal cord, and in the spinal trigeminal tract and the nucleus trigeminus in the lower medulla with lectins specific for terminal alpha-galactose residues. In addition, fibers of the solitary system and the area postrema were reactive with these lectins. The peripheral and central nervous system elements with affinity for galactopyranosyl-specific lectins correspond in distribution with neuroanatomical regions thought to be involved in the transmission and relay of somatic and visceral afferent inputs such as pain and temperature. Such specific localization of a glycosubstance to a distinct subpopulation of neurons and their peripheral and central processes suggests that the particular glycoconjugate may be of physiological significance.