RESUMO
The secretin receptor (SCTR) is a member of Class 2 subfamily B1 GPCRs and part of the PAC1/VPAC receptor subfamily. This receptor has long been known in mammals but has only recently been identified in other vertebrates including teleosts, from which it was previously considered to be absent. The ligand for SCTR in mammals is secretin (SCT), an important gastrointestinal peptide, which in teleosts has not yet been isolated, or the gene identified. This study revises the evolutionary model previously proposed for the secretin-GPCRs in metazoan by analysing in detail the fishes, the most successful of the extant vertebrates. All the Actinopterygii genomes analysed and the Chondrichthyes and Sarcopterygii fish possess a SCTR gene that shares conserved sequence, structure and synteny with the tetrapod homologue. Phylogenetic clustering and gene environment comparisons revealed that fish and tetrapod SCTR shared a common origin and diverged early from the PAC1/VPAC subfamily group. In teleosts SCTR duplicated as a result of the fish specific whole genome duplication but in all the teleost genomes analysed, with the exception of tilapia (Oreochromis niloticus), one of the duplicates was lost. The function of SCTR in teleosts is unknown but quantitative PCR revealed that in both sea bass (Dicentrarchus labrax) and tilapia (Oreochromis mossambicus) transcript abundance is high in the gastrointestinal tract suggesting it may intervene in similar processes to those in mammals. In contrast, no gene encoding the ligand SCT was identified in the ray-finned fishes (Actinopterygii) although it was present in the coelacanth (lobe finned fish, Sarcopterygii) and in the elephant shark (holocephalian). The genes in linkage with SCT in tetrapods and coelacanth were also identified in ray-finned fishes supporting the idea that it was lost from their genome. At present SCTR remains an orphan receptor in ray-finned fishes and it will be of interest in the future to establish why SCT was lost and which ligand substitutes for it so that full characterization of the receptor can occur.
Assuntos
Evolução Molecular , Peixes/genética , Genoma , Receptores Acoplados a Proteínas G/genética , Receptores dos Hormônios Gastrointestinais/genética , Secretina/genética , Sequência de Aminoácidos , Animais , Sequência Conservada/genética , Peixes/metabolismo , Dados de Sequência Molecular , Filogenia , Receptores Acoplados a Proteínas G/classificação , Receptores dos Hormônios Gastrointestinais/classificação , Secretina/metabolismo , Homologia de Sequência de Aminoácidos , Distribuição TecidualRESUMO
Oligomerization of G-protein-coupled receptors (GPCRs) is emerging as a mechanism for regulation and functional modification, although it has been studied most extensively for Family A receptors. Family B receptors have clear structural differences from Family A. In this paper, we have systematically evaluated GPCRs that are capable of association with the prototypic Family B secretin receptor. All of the receptor constructs were shown to traffic normally to the plasma membrane. We utilized receptor bioluminescence resonance energy transfer (BRET) to determine the presence of constitutive and ligand-dependent receptor association. Extensive intra-family and no cross-family association was observed. Of the nine Family B receptors studied, all constitutively yielded a significant BRET signal with the secretin receptor, except for the calcitonin receptor. Each of the associating hetero-oligomeric receptor pairs generated a BRET signal of similar intensity, less than that of homo-oligomeric secretin receptors. BRET signals from some receptor pairs were reduced by ligand occupation, but none were increased by this treatment. Thus, Family B GPCR oligomerization occurs, with many structurally related members associating with each other. The specific functional implications of this need to be further evaluated.
Assuntos
Multimerização Proteica , Receptores Acoplados a Proteínas G/química , Receptores Acoplados a Proteínas G/metabolismo , Receptores dos Hormônios Gastrointestinais/química , Receptores dos Hormônios Gastrointestinais/metabolismo , Sequência de Aminoácidos , Animais , Células COS , Chlorocebus aethiops , Transferência Ressonante de Energia de Fluorescência , Humanos , Dados de Sequência Molecular , Filogenia , Receptores Acoplados a Proteínas G/classificação , Receptores Acoplados a Proteínas G/genética , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/genética , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Secretina/metabolismo , Alinhamento de SequênciaRESUMO
Motilin, a 22-amino acid peptide synthesized in endocrine cells of intestinal mucosa, stimulates GI smooth muscle contractility. To elucidate the mode of action of motilin, we attempted to determine whether motilin receptors are localized on nerve cells or on smooth muscle cells of the GI tract. Mucosa-free tissues from rabbit antrum and duodenum were homogenized separately with a Polytron prior to differential centrifugation to obtain synaptosome or plasma membrane-enriched fractions, as determined by the distribution of [3H]saxitoxin (SAX) binding (neural membranes) and 5' nucleotidase (5'N) activity (smooth muscle plasma membranes). Motilin binding was evaluated by the displacement of [125I]motilin by motilin (1-22) on the various membrane fractions. In the antrum, motilin binding was highly correlated with SAX binding (r = 0.81, p < 0.0005), and also significantly with 5'N activity (r = 0.54, p < 0.05). In the duodenum, motilin binding correlated significantly with 5'N activity (r = 0.67, p < 0.005), but not with SAX binding (r = -0.11, NS). Receptor affinity, for the motilin antagonist MOT(1-12)[CH2NH]10-11, for motilin(1-22), and for the motilin agonist erythromycin lactobionate was significantly (p < 0.001, p < 0.001, and p < 0.05, respectively) higher in SAX-enriched fractions from the antrum than in 5'N-enriched fractions from the duodenum. Therefore, in the rabbit: 1) motilin receptors appear to be predominantly located on nerve tissues in the antrum and restricted to smooth muscle cells in the duodenum, and 2) antral receptors and duodenal receptors displayed different pharmacological characteristics, probably corresponding to two specific and heterogeneous motilin receptor subtypes.
Assuntos
Sistema Digestório/inervação , Sistema Digestório/metabolismo , Motilina/metabolismo , Músculo Liso/inervação , Músculo Liso/metabolismo , Receptores dos Hormônios Gastrointestinais/metabolismo , Receptores de Neuropeptídeos/metabolismo , Sinaptossomos/metabolismo , 5'-Nucleotidase/análise , Animais , Ligação Competitiva , Biomarcadores , Membrana Celular/metabolismo , Duodeno/metabolismo , Feminino , Cinética , Neurônios/metabolismo , Antro Pilórico/metabolismo , Coelhos , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/isolamento & purificação , Receptores de Neuropeptídeos/classificação , Receptores de Neuropeptídeos/isolamento & purificação , Análise de Regressão , Saxitoxina/metabolismoRESUMO
Neuropeptide Y (NPY) belongs to a family of structurally related neuroendocrine peptides that bind to G protein-coupled receptors. Five different receptor subtypes have recently been cloned in mammals and we have found another three receptor genes in the zebrafish, called zYa, zYb, and zYc, that appear to be distinct subtypes as deduced from their widely different sequences. To elucidate the evolutionary relationships between the mammalian and zebrafish receptors, we have used the zebrafish probes to isolate genomic clones from another teleost fish, the Atlantic cod, Gadus morhua. We present here the sequence of the cod Yb gene, whose deduced protein sequence is equally identical to the zebrafish Yb (69%) and Yc proteins (66%). The two zebrafish receptors are 76% identical to each other, suggesting that they arose by gene duplication in the zebrafish lineage after divergence from the cod lineage. The five cloned mammalian NPY-family receptors and the three cloned zebrafish NPY receptors indicate that this is the largest receptor family among all peptide receptors that belong to the superfamily of G protein-coupled receptors.
Assuntos
Peixes/genética , Peixes/metabolismo , Receptores dos Hormônios Gastrointestinais/genética , Receptores de Neuropeptídeo Y/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Primers do DNA/genética , Evolução Molecular , Duplicação Gênica , Mamíferos , Dados de Sequência Molecular , Receptores dos Hormônios Gastrointestinais/classificação , Receptores de Neuropeptídeo Y/classificação , Homologia de Sequência de Aminoácidos , Especificidade da Espécie , Peixe-Zebra/genéticaAssuntos
Proteínas de Drosophila , Proteínas de Ligação ao GTP/fisiologia , Proteínas de Membrana/classificação , Receptores de Superfície Celular/fisiologia , Receptores dos Hormônios Gastrointestinais/classificação , Sequência de Aminoácidos , Animais , Receptores Frizzled , Humanos , Proteínas de Membrana/química , Proteínas de Membrana/fisiologia , Dados de Sequência Molecular , Receptores Acoplados a Proteínas G , Receptores dos Hormônios Gastrointestinais/química , Rodopsina/classificação , Transdução de SinaisRESUMO
The pancreatic polypeptide (PP-fold) family of peptides consists of the endocrine peptides, pancreatic polypeptide (PP) and peptide YY (PYY), and the neuroneally derived peptide, neuropeptide Y (NPY). All three peptides are found in the circulation, with PP found primarily in the pancreas and PYY found principally in the gut. NPY is released into the circulation from neuroneal stores in response to stress. These peptides have broad peripheral actions on a number of organs. Not surprisingly, PYY and PP are believed to play an important role in the function of the gastrointestinal tract while NPY is a potent vasconstrictor and may have effects on the gut through the enteric nervous system. In the brain, NPY has been implicated in anxiety and depression, feeding and obesity, memory retention, neuroneal excitability, endocrine function, and metabolism. Recent advances in the molecular biology of the receptors for these peptides have resulted in the identification of at least six receptor subtypes with varying peptide pharmacology. Compared to other G-protein coupled receptor families, the PP-fold peptide receptors exhibit a relatively low level of sequence identity. Further advances in the development of selective agonists and antagonists for individual receptor subtypes will be needed to understand further their role in physiological function.
Assuntos
Polipeptídeo Pancreático/fisiologia , Receptores dos Hormônios Gastrointestinais/fisiologia , Sequência de Aminoácidos , Animais , Humanos , Dados de Sequência Molecular , Neuropeptídeo Y/fisiologia , Peptídeo YY/fisiologia , Receptores dos Hormônios Gastrointestinais/classificação , Receptores de Neuropeptídeo Y/classificação , Receptores de Neuropeptídeo Y/fisiologiaRESUMO
Neuropeptides have recently been shown to modulate the immune response. Vasoactive intestinal polypeptide (VIP) modulates lymphocyte migration to Peyer's patches and inhibits natural killer cell activity. VIP receptors have been characterized on lymphocytes and have been compared with the VIP receptor in nervous tissue and in tissues of the gastrointestinal tract. The evidence supports the existence of at least two classes of high-affinity VIP receptors as well as a low-affinity receptor. The development of tissue-specific agonists and antagonists to the VIP receptor may thus be feasible.
Assuntos
Glândulas Endócrinas/fisiologia , Sistema Imunitário/fisiologia , Fenômenos Fisiológicos do Sistema Nervoso , Receptores dos Hormônios Gastrointestinais/isolamento & purificação , Peptídeo Intestinal Vasoativo/fisiologia , Animais , Nucleotídeos de Guanina/farmacologia , Humanos , Cinética , Linfócitos/metabolismo , Peso Molecular , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/fisiologia , Receptores de Peptídeo Intestinal VasoativoRESUMO
The Drosophila mutant methuselah (mth) was identified from a screen for single gene mutations that extended average lifespan. Mth mutants have a 35% increase in average lifespan and increased resistance to several forms of stress, including heat, starvation, and oxidative damage. The protein affected by this mutation is related to G protein-coupled receptors of the secretin receptor family. Mth, like secretin receptor family members, has a large N-terminal ectodomain, which may constitute the ligand binding site. Here we report the 2.3-A resolution crystal structure of the Mth extracellular region, revealing a folding topology in which three primarily beta-structure-containing domains meet to form a shallow interdomain groove containing a solvent-exposed tryptophan that may represent a ligand binding site. The Mth structure is analyzed in relation to predicted Mth homologs and potential ligand binding features.
Assuntos
Proteínas de Drosophila , Drosophila/química , Receptores de Superfície Celular/química , Receptores Acoplados a Proteínas G , Sequência de Aminoácidos , Animais , Sítios de Ligação , Cristalografia por Raios X , Expectativa de Vida , Ligantes , Modelos Moleculares , Dados de Sequência Molecular , Filogenia , Conformação Proteica , Receptores de Superfície Celular/classificação , Receptores dos Hormônios Gastrointestinais/química , Receptores dos Hormônios Gastrointestinais/classificação , Homologia de Sequência de AminoácidosRESUMO
Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 +/- 18% increase in basal short-circuit current (Isc) and the induction of Cl- secretion. VIP-stimulated increases in Isc were abolished by the addition of each of PYY, (Pro34)-PYY, a Y1 receptor-selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in Isc. These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro34)-PYY and PYY-(3-36).
Assuntos
Colo/metabolismo , Hormônios Gastrointestinais/fisiologia , Peptídeos/fisiologia , Receptores dos Hormônios Gastrointestinais/fisiologia , Animais , Colo/inervação , Hormônios Gastrointestinais/farmacologia , Transporte de Íons , Masculino , Fragmentos de Peptídeos , Peptídeo YY , Peptídeos/farmacologia , Ratos , Ratos Sprague-Dawley , Receptores dos Hormônios Gastrointestinais/agonistas , Receptores dos Hormônios Gastrointestinais/classificação , Peptídeo Intestinal Vasoativo/farmacologiaRESUMO
In order to explore which amino acids or which blocks of amino acids in the 29 amino acid neuropeptide galanin are important for recognition of the endogenous ligand by galanin receptor subtypes present in the jejunum and in the hypothalamus, respectively, we have carried out L-Ala substitutions of individual amino acids or of blocks of amino acids in the rat galanin sequence and examined the binding of the obtained analogs to the rat hypothalamic and jejunal galanin receptor subtypes. This study reveals that the galanin sequence YLLGPH9-14 is essential for recognition of galanin by both the rat hypothalamic and jejunal galanin receptor subtypes. Substitution of the N-terminal amino acids. GWTL1-4, leads to total loss of affinity of galanin for both hypothalamic and jejunal galanin receptors. The alpha-helical C-terminal amino acid (25-29) part of galanin has no greater influence on the affinity of galanin to the hypothalamic galanin receptor subtype. L-Ala substitution of the C-terminal amino acids of galanin KHGLT25-29 shows, however, that this C-terminal motif is essential for the recognition by the jejunal galanin receptor subtype, whereas amino acids in the middle portion of galanin NSAG5-8 are of importance for binding to the hypothalamic but not to the jejunal receptor. [Ala5-8]Galanin thus has a more than 100-fold higher affinity to jejunal receptor than to the hypothalamic receptor, while [Ala25-29]galanin has a more than 100-fold higher affinity for the hypothalamic than for jejunal galanin receptor subtypes. pH dependence of the galanin binding to these receptor subtypes is also different.
Assuntos
Galanina/metabolismo , Hipotálamo/metabolismo , Jejuno/metabolismo , Receptores dos Hormônios Gastrointestinais/metabolismo , Alanina/química , Sequência de Aminoácidos , Animais , Ligação Competitiva , Galanina/análogos & derivados , Galanina/química , Concentração de Íons de Hidrogênio , Masculino , Ligação Proteica , Conformação Proteica , Ratos , Ratos Sprague-Dawley , Receptores de Galanina , Receptores dos Hormônios Gastrointestinais/classificaçãoRESUMO
Galanin is a peptide hormone which modulates a wide variety of physiological processes, including secretion, muscle contraction, cognitive function, the reproductive axis, and feeding. Two galanin receptor subtypes, GalR1 and GalR2, have been cloned; however, for GalR2 only the rat sequence has been reported in the literature. Our cloning of human GalR2 reveals its amino acid sequence to be 85% identical to rat GalR2 and 39% identical to human GalR1. Binding of [125I]galanin to the human GalR2 receptor transiently expressed in COS-7 cells was saturable (Kd = 0.24 nM +/- 0.06 nM) with a receptor density of 383 +/- 66 fmol/mg protein. Human galanin(1-30) bound with high affinity to the human GalR2 receptor, with a Ki value of 0.86 +/- 0.12 nM. With the identification of a second galanin receptor subtype, the specific functions of human galanin receptor subtypes can now begin to be addressed.
Assuntos
Receptores dos Hormônios Gastrointestinais/química , Sequência de Aminoácidos , Animais , Sequência de Bases , Células COS , Clonagem Molecular , Galanina/metabolismo , Humanos , Radioisótopos do Iodo/metabolismo , Dados de Sequência Molecular , Fragmentos de Peptídeos/metabolismo , Ligação Proteica , RNA Mensageiro/análise , Receptores de Galanina , Receptores dos Hormônios Gastrointestinais/classificação , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Transfecção/genéticaRESUMO
The galanin-receptor ligand M40 [galanin-(1-12)-Pro3-(Ala-Leu)2-Ala amide] binds with high affinity to [mono[125I]iodo-Tyr26]galanin-binding sites in hippocampal, hypothalamic, and spinal cord membranes and in membranes from Rin m5F rat insulinoma cells (IC50 = 3-15 nM). Receptor autoradiographic studies show that M40 (1 microM) displaces [mono[125I]iodo-Tyr26]galanin from binding sites in the hippocampus, hypothalamus, and spinal cord. In the brain, M40 acts as a potent galanin-receptor antagonist: M40, in doses comparable to that of galanin, antagonizes the stimulatory effects of galanin on feeding, and it blocks the galaninergic inhibition of the scopolamine-induced acetylcholine release in the ventral hippocampus in vivo. In contrast, M40 completely fails to antagonize both the galanin-mediated inhibition of the glucose-induced insulin release in isolated mouse pancreatic islets and the inhibitory effects of galanin on the forskolin-stimulated accumulation of 3',5'-cAMP in Rin m5F cells; instead M40 is a weak agonist at the galanin receptors in these two systems. M40 acts as a weak antagonist of galanin in the spinal flexor reflex model. These results suggest that at least two subtypes of the galanin receptor may exist. Hypothalamic and hippocampal galanin receptors represent a putative central galanin-receptor subtype (GL-1-receptor) that is blocked by M40. The pancreatic galanin receptor may represent another subtype (GL-2-receptor) that recognizes M40, but as a weak agonist. The galanin receptors in the spinal cord occupy an intermediate position between these two putative subtypes.
Assuntos
Fragmentos de Peptídeos , Peptídeos/metabolismo , Receptores dos Hormônios Gastrointestinais/classificação , Sequência de Aminoácidos , Animais , Ligação Competitiva , Comportamento Alimentar/fisiologia , Galanina , Insulina/metabolismo , Secreção de Insulina , Ligantes , Masculino , Dados de Sequência Molecular , Peptídeos/farmacologia , Ratos , Ratos Sprague-Dawley , Receptores de Galanina , Receptores dos Hormônios Gastrointestinais/metabolismo , Taxa Secretória/efeitos dos fármacosRESUMO
SUMMARY: All G-protein-coupled receptors (GPCRs) share a common molecular architecture (with seven putative transmembrane segments) and a common signaling mechanism, in that they interact with G proteins (heterotrimeric GTPases) to regulate the synthesis of intracellular second messengers such as cyclic AMP, inositol phosphates, diacylglycerol and calcium ions. Historically, GPCRs have been classified into six families, which were thought to be unrelated; three of these are found in vertebrates. Recent work has identified several new GCPR families and suggested the possibility of a common evolutionary origin for all of them. Family B (the secretin-receptor family or 'family 2') of the GPCRs is a small but structurally and functionally diverse group of proteins that includes receptors for polypeptide hormones, molecules thought to mediate intercellular interactions at the plasma membrane and a group of Drosophila proteins that regulate stress responses and longevity. Family-B GPCRs have been found in all animal species investigated, including mammals, Caenorhabditis elegans and Drosophila melanogaster, but not in plants, fungi or prokaryotes. In this article, I describe the structures and functions of family-B GPCRs and propose a simplified nomenclature for these proteins.
Assuntos
Receptores de Superfície Celular/classificação , Receptores de Superfície Celular/genética , Sequência de Aminoácidos , Animais , Evolução Molecular , Proteínas Heterotriméricas de Ligação ao GTP/metabolismo , Humanos , Dados de Sequência Molecular , Mutação , Filogenia , Estrutura Terciária de Proteína , Receptores de Superfície Celular/química , Receptores de Superfície Celular/fisiologia , Receptores Acoplados a Proteínas G , Receptores dos Hormônios Gastrointestinais/química , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/genética , Alinhamento de Sequência , Terminologia como AssuntoRESUMO
Galanin, a 29-30-amino acid neuropeptide, is widely distributed in central and peripheral systems and mediates a variety of physiological functions. Pharmacological studies have suggested the existence of multiple receptor subtypes but only the type I (GalR1) galanin receptor has been cloned. Now we report the cloning by a combination of sib selection and rapid amplification of cDNA ends of a cDNA encoding a new galanin receptor (GalR2) from rat hypothalamus. The receptor is 372 amino acids in length and shares only 40% homology with the rat GalR1 receptor. It contains seven putative transmembrane domains with the amino and carboxyl termini being least identical to GalR1. Northern blot analyses revealed a 2-kilobase pair mRNA species distributed in several tissues, suggesting a broader functional spectrum than GalR1. 125I-Labeled human galanin binding to rat GalR2 receptor expressed in COS-1 cells was saturable (Kd = 0.59 nM) and could be displaced by galanin, several galanin fragments, and chimeric peptides. The pharmacological profiles of GalR1 and GalR2 receptors were distinguishable by galanin fragment(2-29), which bound the cloned GalR2 receptor with markedly higher affinity than the GalR1 receptor. Activation of the cloned receptor by galanin led to inhibition of forskolin-stimulated intracellular cAMP production. The cloning of this new receptor subtype should provide further insights into the mechanisms by which galanin mediates its diverse physiological functions. The identification of galanin(2-29) as a receptor-specific ligand should enhance the understanding of specificity of galanin-receptor interactions.
Assuntos
Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Células COS/metabolismo , Clonagem Molecular , Colforsina/farmacologia , AMP Cíclico/biossíntese , DNA Complementar/genética , Galanina/metabolismo , Galanina/farmacologia , Humanos , Hipotálamo/química , Hipotálamo/metabolismo , Radioisótopos do Iodo , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Receptores de Galanina , Receptores dos Hormônios Gastrointestinais/genética , Temperatura , TransfecçãoRESUMO
INTRODUCTION: Receptor subtypes can be distinguished by different actions of agonists on physiologic responses. In this study, we compared effects of four species variants of secretin (rat, porcine, canine, and human) on pancreatic secretion and gastrin-induced acid secretion in urethane-anesthetized rats. These secretins differ by one to three residues in position 14, 15, or 16 and were used to probe for the presence of different secretin receptor subtypes in the rat. METHODOLOGY: Pancreatic responses were measured in a two-point parallel line bioassay with porcine secretin (3 and 30 pmol/kg IV bolus) as standard. Inhibition of gastric acid secretion by each secretin (100 pmol/[kg x h]) was quantitated against a threshold dosage of gastrin-17 (200 pmol/[kg x h]), and percent inhibition of incremental acid responses was determined. RESULTS: Rat secretin was significantly more potent than other secretins for pancreatic secretion, in the order of rat > porcine > canine > human. The four secretins significantly inhibited gastrin-induced acid secretion by 37% to 49%, with no statistically significant differences among the forms. CONCLUSIONS: Stimulation of pancreatic secretion was influenced by species variations in secretin structure, but inhibition of gastric acid secretion was not. This finding suggests that secretin receptor subtypes with different recognition patterns mediate these responses.
Assuntos
Pâncreas/metabolismo , Receptores dos Hormônios Gastrointestinais/classificação , Secretina/química , Secretina/farmacologia , Sequência de Aminoácidos , Animais , Cães , Ácido Gástrico/metabolismo , Gastrinas/antagonistas & inibidores , Gastrinas/farmacologia , Humanos , Masculino , Dados de Sequência Molecular , Pâncreas/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Receptores Acoplados a Proteínas G , Receptores dos Hormônios Gastrointestinais/fisiologia , Alinhamento de Sequência , Especificidade da Espécie , SuínosRESUMO
The pancreatic polypeptide family includes neuropeptide Y (NPY), one of the most abundant neuropeptides in the mammalian nervous system, as well as peptide YY (PYY) and pancreatic polypeptide (PP). This peptide family is involved in numerous physiological processes such as memory, pain, blood pressure, appetite, anxiety, and circadian rhythm. Of the multiple Y-type receptors proposed for PP family members, only the Y1 subtype was cloned previously. We now report the isolation of a human Y2 (hhY2) receptor cDNA by expression cloning from a human hippocampal cDNA library, using a 125I-PYY binding assay. hhY2 cDNA encodes a predicted protein of 381 amino acids with low amino acid identity to the human Y1 receptor (31% overall; 41% transmembrane). 125I-PYY binding to transiently expressed hY2 receptors was saturable (pKd = 10.17) and displaceable by human PP family members in rank order: PYY (pKi = 9.47) approximately NPY (pKi = 9.27) >> PP (pKi < 6) and by peptide analogs: NPY2-36 (pKi = 8.80) approximately NPY13-36 (pKi = 8.55) approximately C2-NPY (pKi = 8.54) > NPY26-36 (pKi = 6.51) approximately [Leu31,Pro34]NPY (pKi = 6.23). Human PYY decreased [cAMP] and increased intracellular [Ca2+] in hY2-transfected 293 cells.
Assuntos
Hipocampo/metabolismo , Receptores dos Hormônios Gastrointestinais/genética , Receptores de Neuropeptídeo Y/genética , Sequência de Aminoácidos , Animais , Linhagem Celular , Clonagem Molecular , DNA Complementar/genética , Expressão Gênica , Humanos , Técnicas In Vitro , Cinética , Dados de Sequência Molecular , Polipeptídeo Pancreático/metabolismo , Peptídeo YY , Peptídeos/metabolismo , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/metabolismo , Receptores de Neuropeptídeo Y/classificação , Receptores de Neuropeptídeo Y/metabolismo , Homologia de Sequência de AminoácidosRESUMO
The pancreatic polypeptide family includes pancreatic polypeptide (PP), neuropeptide Y (NPY), and peptide YY (PYY). Members of the PP family regulate numerous physiological processes, including appetite, gastrointestinal transit, anxiety, and blood pressure. Of the multiple Y-type receptors proposed for PP family members, only the Y1 subtype has been cloned previously. We now report the cloning of an additional Y-type receptor, designated Y4, by homology screening of a human placental genomic library with transmembrane (TM) probes derived from the rat Y1 gene. The Y4 genomic clone encodes a predicted protein of 375 amino acids that is most homologous to Y1 receptors from human, rat, and mouse (42% overall; 55% in TM). 125I-PYY binding to transiently expressed Y4 receptors was saturable (pKd = 9.89) and displaceable by human PP family derivatives: PP (pKi = 10.25) approximately PP2-36 (pKi = 10.06) > PYY (pKi = 9.06) approximately [Leu31,Pro34]NPY (pKi = 8.95) > NPY (pKi = 8.68) > PP13-36 (pKi = 7.13) > PP31-36 (pKi = 6.46) > PP31-36 free acid (pKi < 5). Human PP decreased [cAMP] and increased intracellular [Ca2+] in Y4-transfected LMTK- cells. Y4 mRNA was detected by reverse transcriptase-polymerase chain reaction in human brain, coronary artery, and ileum, suggesting potential roles for Y4 receptors in central nervous system, cardiovascular, and gastrointestinal function.
Assuntos
Receptores dos Hormônios Gastrointestinais/genética , Receptores de Neuropeptídeo Y/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Primers do DNA/genética , Feminino , Expressão Gênica , Humanos , Técnicas In Vitro , Masculino , Camundongos , Dados de Sequência Molecular , Neuropeptídeo Y/metabolismo , Polipeptídeo Pancreático/metabolismo , Peptídeo YY , Peptídeos/metabolismo , Ratos , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/metabolismo , Receptores de Neuropeptídeo Y/classificação , Receptores de Neuropeptídeo Y/metabolismo , Homologia de Sequência de AminoácidosRESUMO
Vasoactive intestinal polypeptide (VIP) is the pathophysiologic mediator of several small intestinal hypersecretion states. VIP exerts its effect by binding mucosal receptors and ultimately increasing intracellular levels of cAMP. Peptide YY (PYY), a GI hormone concentrated in the distal ileum and colon, has been demonstrated to decrease VIP-mediated secretion in the colon through a specific Y4 mucosal receptor. Characterization of PYY's effect on VIP-stimulated small intestinal secretion may provide a basis for future therapeutic interventions. We hypothesized that ion transport in the small intestine is mediated through a novel Y receptor subtype. We performed Ussing chamber ion transport studies on rabbit ileum using VIP, PYY, and other pancreatic polypeptide (PP)-fold peptides in order to specifically examine: (1) the effects of VIP and PYY on basal and VIP-stimulated short circuit current (Isc), and (2) the changes in VIP-stimulated Isc in response to NPY, PP, leucine31,proline31 neuropeptide Y fragment, ([Leu31,Pro34]NPY) and the carboxy-terminal fragment of NPY (NPY13-36). VIP increased basal Isc in a concentration-dependent manner, while PYY decreased basal Isc. Graded concentrations of PYY decreased VIP-stimulated increases in Isc. PYY added prior to VIP had no effect on VIP-stimulated increases in ISC. Inhibition of VIP-stimulated Isc increases was seen with NPY, but not with [Leu31,Pro34]NPY, PP, or NPY13-36. This distinct pattern of binding affinity characterizes a novel Y receptor subtype. Additionally, increases in Isc by VIP despite pretreatment with PYY suggests that VIP-stimulated ion transport is mediated through mechanisms other than increases in cAMP.
Assuntos
Íleo/metabolismo , Peptídeos/farmacologia , Peptídeo Intestinal Vasoativo/farmacologia , Animais , Transporte Biológico/efeitos dos fármacos , Condutividade Elétrica , Hormônios Gastrointestinais/farmacologia , Íleo/efeitos dos fármacos , Íleo/fisiologia , Íons , Peptídeo YY , Coelhos , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/fisiologiaRESUMO
Equilibrium binding studies showed that butyrate-treated HT-29 cells express a high-affinity 125I-labeled peptide YY (125I-PYY) binding site with a dissociation constant of 0.32 +/- 0.12 nM (mean +/- SE, n = 4). This site was Y1 preferring because neuropeptide Y (NPY) and the Y1-selective agonist [Leu31,Pro34]NPY were equipotent to PYY at displacing 125I-PYY; PYY-(13-36) and pancreatic polypeptide were > 1,000- and 10,000-fold less potent at displacing the radioligand. PYY and [Leu31,Pro34]NPY inhibited forskolin-stimulated adenosine 3',5'-cyclic monophosphate production 63% and 48%, respectively, with a half-maximal inhibitory concentration between 0.1 and 1.0 nM. PYY and [Leu31,Pro34]NPY had no effect on release of intracellular calcium alone or on the increase in intracellular calcium concentration caused by carbachol or neurotensin. Northern blot analysis of poly(A)+ RNA from HT-29 cells demonstrated a single transcript of 2.5 kb that hybridized to a human Y1-receptor cDNA probe. Sequence analysis of a reverse transcription-polymerase chain reaction product amplified with primers based on human Y1-receptor cDNA confirmed that these cells contained mRNA encoding the human Y1 receptor. These studies show that butyrate-treated HT-29 cells constitutively express the Y1-preferring NPY/PYY receptor and Y1 mRNA and provide a new model for studies of PYY-regulated epithelial cell function and tissue-specific expression of the human Y1-receptor gene.
Assuntos
Mucosa Intestinal/metabolismo , Receptores dos Hormônios Gastrointestinais/classificação , Receptores dos Hormônios Gastrointestinais/metabolismo , Receptores de Neuropeptídeo Y/classificação , Receptores de Neuropeptídeo Y/metabolismo , Sítios de Ligação , Northern Blotting , Butiratos/farmacologia , Ácido Butírico , Cálcio/metabolismo , Linhagem Celular , AMP Cíclico/antagonistas & inibidores , Hormônios Gastrointestinais/farmacologia , Humanos , Mucosa Intestinal/citologia , Mucosa Intestinal/efeitos dos fármacos , Membranas Intracelulares/metabolismo , Peptídeo YY , Peptídeos/farmacologia , Reação em Cadeia da Polimerase , Transcrição GênicaRESUMO
Only one secretin receptor has been cloned and its properties characterized in native and transfected cells. To test the hypothesis that stimulatory and inhibitory effects of secretin are mediated by different secretin receptor subtypes, pancreatic and gastric secretory responses to secretin and secretin-Gly were determined in rats. Pancreatic fluid secretion was increased equipotently by secretin and secretin-Gly, but secretin was markedly more potent for inhibition of basal and gastrin-induced acid secretion. In Chinese hamster ovary cells stably transfected with the rat secretin receptor, secretin and secretin-Gly equipotently displaced (125)I-labeled secretin (IC(50) values 5.3 +/- 0.5 and 6.4 +/- 0.6 nM, respectively). Secretin, but not secretin-Gly, caused release of somatostatin from rat gastric mucosal D cells. Thus the equipotent actions of secretin and secretin-Gly on pancreatic secretion appear to result from equal binding and activation of the pancreatic secretin receptor. Conversely, secretin more potently inhibited gastric acid secretion in vivo, and only secretin released somatostatin from D cells in vitro. These results support the existence of a secretin receptor subtype mediating inhibition of gastric acid secretion that is distinct from the previously characterized pancreatic secretin receptor.