RESUMO
Flagellated anaerobic motile spiral bacteria were isolated from the faeces of two patients with diarrhoea. They were recovered by the microaerophilic culture method used to detect campylobacters but demanded anaerobic conditions for subculture. Electron microscopy and other investigations showed them to be closely related to Anaerobiospirillum succiniproducens first described in beagle dogs and subsequently in three humans with bacteraemia.
Assuntos
Diarreia/microbiologia , Spirillum/isolamento & purificação , Adulto , Pré-Escolar , Meios de Cultura , Fezes/microbiologia , Fermentação , Humanos , Masculino , Testes de Sensibilidade Microbiana , Microscopia Eletrônica , Spirillum/metabolismo , Spirillum/ultraestruturaRESUMO
Biomimetics is a newly emerging interdisciplinary field in materials science and engineering and biology in which lessons learned from biology form the basis for novel technological materials. It involves investigation of both structures and physical functions of biological composites of engineering interest with the goal of designing and synthesizing new and improved materials. This paper discusses microarchitectural aspects of some structural biocomposites, presents microstructural criteria for future materials design and processing, and identifies areas of future research.
Assuntos
Materiais Biocompatíveis/química , Microscopia Eletrônica , Animais , Carbonato de Cálcio/química , Cerâmica , Colágeno/ultraestrutura , Spirillum/ultraestrutura , Tendões/ultraestruturaRESUMO
A spiral shaped bacterium was seen in smears and histological sections (stained by carbolfuchsin) of gastric, ileal and caecal mucosa as well as in stool smears from mice. A significant correlation between the presence of the spiral bacterium and the occurrence of gastritis was observed but the ileal and caecal mucosa seemed unaffected. The bacterium was Gram negative and grew on BHM and Skirrow's medium, under microaerophilic conditions, at 37 degrees C. Its major biochemical characteristics included positive catalase and oxidase reactions and a rapidly positive urease test. There were 2 or 3 spiral turns per cell and a tuft of up to 12 sheathed flagella on each pointed end. Entwined, braided periplasmic fibrils covered the surface of the cell. This spiral bacterium seemed to be part of the normal intestinal flora but was associated with gastritis.
Assuntos
Mucosa Gástrica/microbiologia , Gastrite/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Mucosa Intestinal/microbiologia , Spirillum/isolamento & purificação , Animais , Ceco/microbiologia , Mucosa Gástrica/ultraestrutura , Íleo/microbiologia , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica , Spirillum/ultraestruturaRESUMO
Twenty isolates of N2-fixing spirilla were isolated from the rhizosphere of maize and sugar cane grown in Egyptian and Belgian soils. Electron microscopy distinguished two morphological groups. The first includes short and thick curved rods with an unipolar flagellum while cells of the second group are much longer with the typical appearance of spiral cells and most probably possess a bipolar tuft of flagella.
Assuntos
Spirillum/ultraestrutura , Bélgica , Egito , Flagelos/ultraestrutura , Fixação de Nitrogênio , Plantas Comestíveis/microbiologia , Microbiologia do Solo , Spirillum/metabolismo , Zea mays/microbiologiaAssuntos
Proteínas de Bactérias/análise , Spirillum/ultraestrutura , Membrana Celular/análise , Membrana Celular/fisiologia , Membrana Celular/ultraestrutura , Eletroforese em Gel de Poliacrilamida , Métodos , Peso Molecular , Oxirredução , Peptídeo Hidrolases , Peptídeos/análise , Peptidoglicano/análise , Fosfolipídeos/fisiologia , Ligação Proteica , TripsinaRESUMO
New microaerophilic sulfur-oxidizing spirilla were isolated from hydrogen sulfide sludge of wastewater treatment plants. Strains D-427 and D-430 have spiral cells that are highly motile due to bipolar flagellum bundles covered with mucous sheaths. Under a phase-contrast microscope, these bundles are visible as single polar flagella. Spheroplasts are formed in the stationary growth phase. Both strains are obligate organotrophs able to oxidize a number of reduced sulfur compounds. The oxidation of sulfide and polysulfide leads to the formation of intracellular globules of elemental sulfur; thiosulfate oxidation results in tetrathionate accumulation in the medium. The cells are unable to utilize reduced sulfur compounds in the energy metabolism; their oxidation is caused by a chemical interaction with H2O2 and O2, synthesized in the electron transport chain. Both strains are obligate microaerophiles with an optimal oxygen concentration in the gas phase of 2 and 0.8% for strains D-427 and D-430, respectively. The strains utilize a limited number of organic acids as growth substrates, mainly tricarboxylic-acid-cycle intermediates. The DNA G+C content is 38.0 mol % (T(m)) for strain D-427 and 38.9 mol % for strain D-430. Phylogenetic analysis, based on the comparison of 16S rRNA gene sequences, revealed that the new isolates of sulfur spirilla are the most closely related to Spirillum volutans, the type species of the genus (97.4% similarity). They were assigned to the genus Spirillum within the class Beta-proteobacteria as two new species, S. winogradskii sp. nov. (D-427T = DSM 12756T) and S. kriegii sp. nov. (B-430T = BKM B-2372T). The emended description of the genus Spirillum is provided.
Assuntos
Esgotos/microbiologia , Spirillum/metabolismo , Spirillum/ultraestrutura , Enxofre/metabolismo , Microbiologia da Água , Genótipo , Oxigênio/farmacologia , Filogenia , Spirillum/isolamento & purificação , Sulfetos/metabolismoRESUMO
The cell surface of Aquaspirillum bengal was devoid of superficial wall structure. When cells were freeze-cleaved and etched, multiple fracture planes through the cell envelope were exposed, which was unusual for an aquaspirillum. These included both convex and concave surfaces and they are compared with those of other gram-negative bacteria.
Assuntos
Spirillum/ultraestrutura , Parede Celular/ultraestrutura , Técnica de Congelamento e RéplicaRESUMO
Electron microscopy of thin-sectioned Spirillum volutans (ATCC 19554) showed that at the insertion site of the flagellum there was a cylindrical structure with a diameter of ca. 36 nm which extended ca. 19 nm into the cytoplasm. This structure, termed a cytoplasmic flagellar base, enclosed a central rod which was continuous with the hook. There was a continuation of the flagellar base into the peptidoglycan layer, enclosing ringlike structures and the central rod. The flagellar hook and proximal part of the flagellar filament contained a central channel which was large enough to accommodate the flagellin subunit. The flagella of fixed cells may project perpendicularly from the outer membrane in a position corresponding to a trailing, swimming orientation or may bend almost parallel to the membrane in a leading orientation. Maximum bending occurred in the hook region, which may be the structure responsible for executing changes in swimming direction.
Assuntos
Spirillum/ultraestrutura , Flagelos/ultraestrutura , Microscopia Eletrônica , Microscopia Eletrônica de VarreduraRESUMO
In unipolar cells of Spirillum volutans, the flagellar rotation frequency is halved, approximately, when the flagellar bundle reorientates to rotate about the cell body and reverse the swimming direction. The viscous drag resulting from a concomitant increase in flagellar wave amplitude is probably responsible for the reduced frequency of flagellar rotation.
Assuntos
Flagelos/fisiologia , Spirillum/ultraestrutura , Movimento , Spirillum/fisiologiaRESUMO
Low temperature transmission electron microscopy can be used to study the structure of biological materials in the hydrated state. Spatial averaging techniques are necessary to overcome the radiation damage problems, and for this reason the techniques described are most applicable to crystalline objects. However, with thin, crystalline biological specimens there are no difficulties with preserving periodicity during the freezing process. Improved specimen preparation methods are described which achieve the production of very thin aqueous films with the hydrated specimen embedded in them. Defocused bright field images of frozen, hydrated protein crystals possess a surprisingly high contrast, presumably due to the difference in density of the protein and the aqueous phase. The techniques described have been used to study the crystal structure of hydrated catalase and the outermost cell wall of Spirillum serpens.
Assuntos
Congelamento , Microscopia Eletrônica , Catalase/análise , Parede Celular/ultraestrutura , Cristalografia , Spirillum/ultraestruturaRESUMO
The backing layer of the Spirillum serpens VHA cell wall, which supports and is bonded to the outer, structured protein layer, was isolated and shown to be similar in composition to the same elements of the outer membrane. It contained a lipopolysaccharide that was similar, but not identical, to that of the intact wall and the same phospholipids. The interaction of the isolated wall lipopolysaccharide with the loosely bound wall lipids provided lamellae, whose surfaces were an effective template for a lifelike reassembly of the isolated outer-layer hexagonal protein in the presence of Ca2+. Assembly did not take place on pure lipopolysaccharide, which dispersed in differing forms. A lipid-lipopolysaccharide-water interface appeared to be required as a template surface for the assembly. Lipopolysaccharide from Pseudomonas aeruginosa was able to replace that of S. serpens in the template. These observations suggest that lipid-lipopolysaccharide complexes are highly ordered, and this order is important to the nucleation and assembly of the protein array.
Assuntos
Proteínas de Bactérias , Parede Celular/ultraestrutura , Lipopolissacarídeos , Polissacarídeos Bacterianos , Spirillum/ultraestrutura , Microscopia Eletrônica , FosfolipídeosRESUMO
A complex and easily disrupted arrangement of macromolecules was present on the outer (lipopolysaccharide) membrane of the cell wall of Spirillum metamorphum. Separation of the arrays from the cell and spontaneous reassembly into regularly structured complexes usually occurred during preparation for electron microscopy. Freeze etchings, thin sections, and optical diffraction analysis of negatively stained fragments indicated that they consisted of two sets of a thin layer which was studied with 3-nm particles arranged in a loose (OL). The OSL consisted of a hexagonal arrangement of 8-nm disks and the OL of a thin layer which was studied with 3-nm particles arranged in a loose rectangular manner. The OSL of reassembled fragments displayed numerous broken delta-linkers between units and a center-to-center spacing of half the expected distance, which suggests that an interdigitation of two OSL arrays had occurred. The observations combined with freeze etchings and thin sections of whole cells suggested a possible reassembly mechanism. The normal surface arrangement of these layers on cells was thought to consist of the OL overlying one set of OSL which was loosely adherent to a thin amorphous backing layer.
Assuntos
Spirillum/ultraestrutura , Membrana Celular/ultraestrutura , Parede Celular/ultraestrutura , Técnica de Congelamento e Réplica , Lipopolissacarídeos , Microscopia Eletrônica , Modelos Estruturais , Polissacarídeos BacterianosRESUMO
Campylobacter pylori may not be the only organism that causes active chronic gastritis in man. We report two cases of gastric infection with a spiral organism distinct from C. pylori. The first patient is a 36-year-old female who presented with epigastric pain and abdominal colic present since childhood and who had 14 cats. Endoscopy was normal. The second patient kept two dogs. Histology of gastric mucosal biopsy specimens in both patients revealed active chronic gastritis, most severe in body mucosa. Giemsa stain revealed bacteria with four to eight spirals, 0.5 micron in diameter and 3-7 micron in length. The organisms had multiple sheathed flagella at the pole and smooth cell walls without axial filaments. The organisms resembled the gastric spirillum that has been seen in cats, dogs, and nonhuman primates. After antibacterial therapy with bismuth subsalicylate, amoxicillin, and metronidazole, the organisms disappeared in both patients and the gastritis healed. Unlike C. pylori, this new spirillum prefers to colonize gastric mucosa containing parietal cells. Whereas this type of organism is a common commensal in other mammals, it appears to be associated with and a possible cause of gastritis in humans.
Assuntos
Gastrite/microbiologia , Spirillum/ultraestrutura , Adulto , Idoso , Feminino , Gastroscopia , Humanos , Masculino , Microscopia Eletrônica , Estômago/ultraestruturaRESUMO
The structure of the regular surface layer of Aquaspirillum serpens MW5 has been investigated by electon microscopy supplemented by computer image processing and least-squares analysis. The layer has a ribbed appearance, both on the bacterium and in isolated, negatively stained fragments. However, detailed analysis indicated that the layer was composed of two hexagonal sheets having p6mm symmetry and a = 16 nm. One sheet was staggered by one half repeat along a (1,0) line of the p6nm lattice relative to the second so that, in projection, the pattern of the composite layer was a translational moiré, characterized by a series of ribs spaced 16 nm apart. The ribbed layer had cmm symmetry with a = 32 nm and b = 18.5 nm. Analysis of this pattern indicated that the two p6nm hexagonal sheets were unevenly stained, and this was confirmed by using least-squares methods to simulate the observed pattern by combining two hexagonal patterns. The general structure of the layer was consistent with a role as a selective and protective barrier on the cell surface.
Assuntos
Spirillum/ultraestrutura , Membrana Celular/ultraestrutura , Parede Celular/ultraestrutura , Computadores , Análise de Fourier , Interferometria , Microscopia Eletrônica , FotogrametriaRESUMO
A long (20-30 micrometer), wide (3-5 micrometer) microbial-mat bacterium from the Ebro Delta (Tarragona, Spain) was grown in mixed culture and videographed live. Intracellular elemental sulfur globules and unique cell termini were observed in scanning-electron-microprobe and transmission-electron micrographs. A polar organelle underlies bundles of greater than 60 flagella at each indented terminus. These Gram-negative bacteria bend, flex, and swim in a spiral fashion; they translate at speeds greater than 10 body lengths per second. The large size of the spirillum permits direct observation of cell motility in single individual bacteria. After desiccation (i.e., absence of standing water for at least 24 h), large populations developed in mat samples remoistened with sea water. Ultrastructural observations reveal abundant large sulfur globules irregularly distributed in the cytoplasm. A multilayered cell wall, pliable and elastic yet rigid, distends around the sulfur globules. Details of the wall, multiflagellated termini, and large cytoplasmic sulfur globules indicate that these fast-moving spirilla are distinctive enough to warrant a genus and species designation: Titanospirillum velox genus nov., sp. nov. The same collection techniques at a similar habitat in the United States (Plum Island, northeast Essex County, Massachusetts) also yielded large populations of the bacterium among purple phototrophic and other inhabitants of sulfurous microbial-mat muds. The months-long survival of T. velox from Spain and from the United States in closed jars filled with mud taken from both localities leads us to infer that this large spirillum has a cosmopolitan distribution.
Assuntos
Spirillum/isolamento & purificação , Enxofre/metabolismo , Spirillum/metabolismo , Spirillum/ultraestruturaRESUMO
The cell envelope of Sporillum sp. strain "Ordal" (possibly a variety of S. anulus) demonstrated multiple superficial wall layers which were diverse in their macromolecular arrays. Negative staining and freeze-etching techniques revealed an outer hexagonally packed layer and an inner tetragonally packed layer. However, both thin sections and freeze-etched cleavages of the wall showed that each of these regular structures rested upon a backing layer, and that there was a delicate amorphous layer overlying the outer hexagonal array. Rotary integration, optical deffraction, and reconstruction of image were used to clarify measurements of each array and to verify the validity of a diagrammatic model of the outer hexagonal system. The integrity of these layers required suitable cations (Ca2+ appeared essential) and pH (pH less than or equal to 4.6 dissociated most superficial layers). These observations aided in the development of a low-pH cationic-substitution technique, in which Na+ replaced essential Ca2+, for extraction of the layers from the cell surface. Dialysis to remove Na+ and restoration of Ca2+ initiated in vitro reassembly of the superficial layer components until regularly structured assembly products were formed.
Assuntos
Spirillum/ultraestrutura , Cálcio , Parede Celular/ultraestrutura , Técnica de Congelamento e Réplica , Concentração de Íons de Hidrogênio , Modelos Estruturais , Sódio , Spirillum/crescimento & desenvolvimentoRESUMO
Membrane protein patterns in SDS-PAGE clearly characterized Azospirillum amazonense, A. brasilense, A. lipoferum, and Herbaspirillum seropedicae. Each species showed also a typical major band with approximate molecular weights (AMWs) of 44, 39.5, 35.5, and 37.5 Kdaltons for the four species respectively. Characteristic lighter bands with AMWs of 63.5 and 19 Kdaltons were shown by A. amazonense. Similar bands of 54, 24 and 24.5 Kdaltons were typical of A. brazilense, A. lipoferum and H. seropedicae, respectively. The three Azospirillum species showed four identical proteins in common, out of the six main proteins presented in SDS-PAGE; H. seropedicae had only two major proteins with one of them common to all three Azospirillum species. The data strongly favors the maintenance of Azospirillum and Herbaspirillum as valid generic taxa as well as supports the validity of the three Azospirillum species.
Assuntos
Proteínas de Membrana/ultraestrutura , Spirillum/ultraestrutura , Azospirillum brasilense/ultraestrutura , Eletroforese em Gel de Poliacrilamida , Proteínas de Membrana/isolamento & purificaçãoRESUMO
Light and electron microscopy showed gastric spirilla in the gastric mucosa of 45 clinically normal rhesus monkeys (Macaca mulatta). In paraffin sections, gastric spirilla were best shown by silver impregnation stains. When stained by hematoxylin and eosin (HE), gastric spirilla may be mistaken for strands of mucus. In thick sections of epon-embedded tissue, gastric spirilla looked like "corkscrews" with up to 12 coils. They were 8 microns long and 0.7 micron wide, and had characteristic bipolar flagella. They were concentrated in the gastric glands at the isthmus, were less common at the neck and base, and were absent in the gastric lumen. Gastric spirilla were associated closely with parietal cells and could penetrate their cytoplasm. Gastric spirilla elicit neither changes of host cytocomponents nor an inflammatory response in the gastric mucosa.
Assuntos
Mucosa Gástrica/microbiologia , Macaca mulatta/microbiologia , Spirillum/ultraestrutura , Animais , Feminino , Flagelos/ultraestrutura , Masculino , Microscopia Eletrônica/veterináriaRESUMO
Microaerophilic spiral organisms may be isolated frequently from samples of gastric mucus taken from patients undergoing gastroscopy. The ultrastructure of these gastric campylobacter-like organisms ('Campylobacter pyloridis') shows that they have greater affinities with Spirillum than with Campylobacter.