Assuntos
Dessensibilização Imunológica , Tumor do Seio Endodérmico/terapia , Filgrastim/efeitos adversos , Fármacos Hematológicos/efeitos adversos , Neoplasias Vaginais/terapia , Neutropenia Febril Induzida por Quimioterapia/etiologia , Neutropenia Febril Induzida por Quimioterapia/prevenção & controle , Pré-Escolar , Tumor do Seio Endodérmico/complicações , Tumor do Seio Endodérmico/diagnóstico , Tumor do Seio Endodérmico/imunologia , Feminino , Filgrastim/administração & dosagem , Filgrastim/imunologia , Fármacos Hematológicos/administração & dosagem , Fármacos Hematológicos/imunologia , Humanos , Resultado do Tratamento , Neoplasias Vaginais/complicações , Neoplasias Vaginais/diagnóstico , Neoplasias Vaginais/imunologiaRESUMO
Clear cell carcinoma (CCC) of the ovary is the surface epithelial neoplasm most often confused with primitive germ cell tumors, particularly yolk sac tumor (YST) and dysgerminoma. OCT3/4 has proven to be a sensitive and relatively specific marker for the latter entity, but existing markers for YST are limited. Recent studies suggest that glypican-3 (GPC3), an oncofetal protein expressed in fetal liver and malignant tumors of hepatocytic lineage, is also expressed in germ cell tumors, particularly YST. To investigate whether GPC3 is useful in distinguishing YST from ovarian CCC, we studied the expression of GPC3 in a large series of ovarian neoplasms and compared it to the expression profiles of CK7 and alpha-fetoprotein. Tissue microarrays containing over 400 benign and malignant ovarian neoplasms, including 34 CCCs were stained with monoclonal GPC3 (clone 1G12, Biomosaics, Burlington, VT). These arrays contained a wide assortment of ovarian surface epithelial neoplasms and sex cord stromal neoplasms, as well as germ cell tumors. Full paraffin tissue sections from 32 YSTs and 10 CCCs were also assessed. All but one YST (97%), including those associated with mixed germ cell tumor were positive for GPC3, whereas all teratomas and embryonal carcinomas were negative. Both cytoplasmic and membrane staining were present in the positive cases, with no background staining. The syncytiotrophoblastic cells in the germ cell tumors and placental villi included in the arrays were also positive for GPC3. Most CCCs (83%) were completely negative for GPC3, as were 99% serous, 94% endometrioid, and 100% mucinous tumors. Five CCCs exhibited focal, moderate to strong GPC3 expression and in 2 the expression was focal and weak. All other tissues, including normal ovary were negative for GPC3. GPC3 seems to be a promising diagnostic marker for differentiating YST from ovarian CCC (P < 0.0001). Because GPC3 may be associated with alpha-fetoprotein expression, further studies are required to determine the utility of GPC3 in differentiating YST from CCC with hepatoid differentiation.
Assuntos
Antígenos de Neoplasias/análise , Biomarcadores Tumorais/análise , Carcinoma/química , Tumor do Seio Endodérmico/química , Glipicanas/análise , Neoplasias Ovarianas/química , Carcinoma/imunologia , Carcinoma/patologia , Diagnóstico Diferencial , Tumor do Seio Endodérmico/imunologia , Tumor do Seio Endodérmico/patologia , Feminino , Humanos , Imuno-Histoquímica , Queratina-7/análise , Neoplasias Ovarianas/imunologia , Neoplasias Ovarianas/patologia , Valor Preditivo dos Testes , Sensibilidade e Especificidade , Análise Serial de Tecidos , alfa-Fetoproteínas/análiseRESUMO
The clinical, morphological, and immunohistochemical findings in six cases of ovarian endometrioid tumors (five endometrioid carcinomas and one carcinosarcoma) with a yolk sac tumor (YST) component are described. The age of the patients ranged from 31 to 73 years (average, 53), and only two patients were premenopausal. Two cases were stage Ia tumors, three stage III, and one stage IV. A substantial postoperative elevation of alpha-fetoprotein (AFP) was seen in two patients and a mild increase in another two. All six patients had surgery and postoperative cisplatin-based chemotherapy regimens, four of whom died of tumor 3 to 14 months after surgery without response to treatment. Only a stage Ia patient is alive and well 1 year after surgery. The tumors were large (average, 17 cm). Benign endometrioid lesions were found in the homolateral ovary in two cases and in the contralateral ovary in another two. All cases had endometrioid ovarian carcinomas (EOC) of various types admixed with typical YST components. Immunohistochemically, EOC areas differed from YST in their positivity for OC 125, CA 19.9, and nuclear estrogen and progesterone receptors and in their negativity for AFP, which was conspicuously positive in the YST areas. The clinicopathological profile of ovarian endometrioid tumors with YST also differs from that of YST in that it occurs in the same age range as EOC, it shows coexistence of benign endometrioid lesions, and it has a poor response to chemotherapy. The histological pattern in transitional areas may be difficult to differentiate from "endometrioid-like" (enteroblastic) YST and clear cell tumors. Ovarian endometrioid tumors with YST component should be considered a variant of endometrial carcinoma. Its recognition is necessary in view of its unusually aggressive behavior and poor prognosis.
Assuntos
Carcinoma Endometrioide/patologia , Tumor do Seio Endodérmico/patologia , Neoplasias Ovarianas/patologia , Adulto , Idoso , Antígeno CA-19-9/análise , Carcinoma Endometrioide/diagnóstico , Carcinoma Endometrioide/imunologia , Tumor do Seio Endodérmico/diagnóstico , Tumor do Seio Endodérmico/imunologia , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/imunologia , Receptores de Estrogênio/análise , Receptores de Progesterona/análise , alfa-Fetoproteínas/análiseRESUMO
Expression of CD30 antigen using the monoclonal antibody Ber-H2 was investigated in 52 testicular tumors: pure seminoma (19 cases), pure embryonal carcinoma (eight cases), and mixed germ cell tumors (25 cases). None of the seminomas expressed Ber-H2, whereas seven of eight pure embryonal carcinomas contained this antigen. In mixed germ cell tumors Ber-H2 was present in none of 10 seminomatous components, none of seven yolk sac tumor components, and 20 of 24 embryonal carcinoma components. Teratomatous elements (16 cases) failed to stain with Ber-H2. Also, foci of intratubular germ cell neoplasia (ITGCN) (31 cases) failed to express Ber-H2. Ber-H2 is useful in distinguishing embryonal carcinoma from seminoma and yolk sac tumor. The failure of expression of Ber-H2 in ITGCN, as in seminoma, supports the close relationship of seminoma with ITGCN.
Assuntos
Antígeno Ki-1/análise , Neoplasias Embrionárias de Células Germinativas/imunologia , Neoplasias Testiculares/imunologia , Adolescente , Adulto , Idoso , Anticorpos Monoclonais , Carcinoma Embrionário/imunologia , Tumor do Seio Endodérmico/imunologia , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , Seminoma/imunologiaRESUMO
Renal cell carcinoma antigen is a rather specific marker for normal and neoplastic renal tissue. We investigated the expression of this antigen in 34 gonadal and extragonadal germ cell tumors, including 8 pure yolk sac carcinomas and 26 embryonal carcinomas, 15 of which were combined with teratomas, seminomas, and dysgerminomas. Renal cell carcinoma antigen was demonstrated in all 8 yolk sac tumors and 21 of 26 embryonal carcinomas (81%). In yolk sac tumors, renal cell carcinoma antigen reactivity was diffusely present throughout the tumors. In embryonal carcinomas, this marker was identified only in yolk sac components. Both intracytoplasmic and membranous staining patterns were present. No reactivity was noticed in embryonal carcinoma cells, seminoma, dysgerminoma, and other components of teratomas. The study suggests an antigenic similarity between renal tubules and yolk sac tumors. Furthermore, the renal cell carcinoma antigen may be used as an addition to the panel of immunocytochemical markers for yolk sac carcinomas.
Assuntos
Antígenos de Neoplasias/análise , Carcinoma Embrionário/imunologia , Tumor do Seio Endodérmico/imunologia , Adolescente , Adulto , Anticorpos Monoclonais , Carcinoma Embrionário/patologia , Carcinoma de Células Renais/imunologia , Carcinoma de Células Renais/patologia , Tumor do Seio Endodérmico/patologia , Feminino , Germinoma/imunologia , Germinoma/patologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Proteínas Quinases Ativadas por Mitógeno/análise , Saco Vitelino/imunologia , Saco Vitelino/patologiaRESUMO
The immunohistochemical expression of type 1 blood group antigens (type 1 BGAs) was analyzed for 30 cases of testicular germ cell tumors (TGCTs), using monoclonal antibodies against DU-PAN-2, CA19-9, Lewis(a) (Le(a)), and Lewis(b) (Le(b)). DU-PAN-2 was expressed very frequently in all of the embryonal carcinomas (ECs). CA19-9 expression was demonstrated in 53% of ECs, but the number of positive cells was generally smaller than that for DU-PAN-2. CA19-9-negative ECs tended to show a higher number of DU-PAN-2-positive cells compared to CA19-9-positive ECs, and ECs in which DU-PAN-2 was more strongly expressed showed a relatively frequent expression of CA19-9. In 36% of seminomas and 56% of yolk sac tumors (YSTs), DU-PAN-2 was weakly expressed, and the positive cells were few in number. Little or no expression of CA19-9 was demonstrated in seminomas and YSTs. Regarding Le(a) and Le(b), the expressions were found to be limited to teratomas at a frequency of 57% and 86%, respectively, with the exception of one EC positive for Lea and one YST positive for Leb. Eighty-six percent of teratomas showed expressions of DU-PAN-2 and CA19-9. DU-PAN-2 was also seen in some intratubular malignant germ cells. The antibodies used were all negative for choriocarcinomas, syncytiotrophoblastic giant cells, and normal testicular tissues. The antigen expressions were predominantly observed on the surface of tumor cells developing luminal structures. In conclusion, although CA19-9 was relatively specific for ECs, it should be emphasized that ECs were rather characteristic of extensive DU-PAN-2 expression. Particularly in CA19-9-negative ECs, a combined analysis of DU-PAN-2 and CA19-9 would be helpful in confirming the histopathologic diagnosis of TGCTs. The clinical significance of DU-PAN-2 in ECs as a tumor marker remains to be clarified. Le(a) and Le(b) expressions were thought to be related to the differentiation or maturation rather than to the malignant transformation in TGCTs.
Assuntos
Antígenos de Neoplasias/metabolismo , Biomarcadores Tumorais/metabolismo , Neoplasias Embrionárias de Células Germinativas/metabolismo , Neoplasias Testiculares/metabolismo , Adolescente , Adulto , Anticorpos Monoclonais , Antígeno CA-19-9/metabolismo , Antígeno Carcinoembrionário/metabolismo , Carcinoma Embrionário/imunologia , Carcinoma Embrionário/metabolismo , Carcinoma Embrionário/patologia , Criança , Pré-Escolar , Tumor do Seio Endodérmico/imunologia , Tumor do Seio Endodérmico/metabolismo , Tumor do Seio Endodérmico/patologia , Feminino , Humanos , Técnicas Imunoenzimáticas , Lactente , Recém-Nascido , Antígenos do Grupo Sanguíneo de Lewis/metabolismo , Masculino , Pessoa de Meia-Idade , Neoplasias Embrionárias de Células Germinativas/imunologia , Neoplasias Embrionárias de Células Germinativas/patologia , Orquiectomia , Seminoma/imunologia , Seminoma/metabolismo , Seminoma/patologia , Neoplasias Testiculares/imunologia , Neoplasias Testiculares/patologiaRESUMO
Endodermal sinus tumor is a malignant germ cell tumor which usually arises in gonads. Extragonadal endodermal sinus tumors in the head and neck are very rare. Here a gingival endodermal sinus tumor is reported. The lesion demonstrated typical microscopic features of the endodermal sinus tumors of gonads. The tumor cells showed alpha-fetoprotein immunoreactivity in immunohistochemical evaluation. The serum alpha-fetoprotein level was high. This is the first gingival case in the related literature.
Assuntos
Tumor do Seio Endodérmico/patologia , Neoplasias Gengivais/patologia , Biópsia , Tumor do Seio Endodérmico/sangue , Tumor do Seio Endodérmico/imunologia , Evolução Fatal , Feminino , Neoplasias Gengivais/sangue , Neoplasias Gengivais/imunologia , Humanos , Imuno-Histoquímica , Lactente , alfa-Fetoproteínas/análiseRESUMO
No disponible
Assuntos
Humanos , Feminino , Pré-Escolar , Dessensibilização Imunológica , Tumor do Seio Endodérmico/terapia , Filgrastim/efeitos adversos , Fármacos Hematológicos/efeitos adversos , Neoplasias Vaginais/terapia , Neutropenia Febril Induzida por Quimioterapia/etiologia , Neutropenia Febril Induzida por Quimioterapia/prevenção & controle , Filgrastim/administração & dosagem , Filgrastim/imunologia , Fármacos Hematológicos/administração & dosagem , Fármacos Hematológicos/imunologia , Resultado do Tratamento , Neoplasias Vaginais/imunologia , Tumor do Seio Endodérmico/imunologiaAssuntos
Anticorpos/imunologia , Antígenos/imunologia , Tumor do Seio Endodérmico/imunologia , Tumor do Seio Endodérmico/patologia , Peroxidase do Rábano Silvestre/química , Animais , Anticorpos/química , Reações Antígeno-Anticorpo , Antígenos/química , Técnica Indireta de Fluorescência para Anticorpo , Camundongos , gama-Globulinas/química , gama-Globulinas/imunologiaRESUMO
A series of five endodermal sinus tumors was studied for their cytoskeletal and other phenotypic markers. They included 2 ovarian, 2 testicular, and 1 inguinal tumors. The cytoskeletal expression was also studied by gel electrophoresis and immunoblotting. Every tumor was diffusely and strongly immunostained for cytokeratin. By SDS-PAGE and immunoblotting, cytokeratins 8 & 18 were detected. Vimentin was focally coexpressed in 4 cases. The stroma was diffusely immunostained for vimentin. None of them expressed desmin, neurofilament, or glial filament protein. Desmoplakin was expressed only in one ovarian tumor. Alpha-fetoprotein and S-100 protein were also diffusely positive among the neoplastic cells; intracytoplasmic globules were especially strongly immunostained. These findings suggest that endodermal sinus tumors represent a group of pure malignant epithelial neoplasms, and may be regarded as primitive carcinomas.
Assuntos
Proteínas do Citoesqueleto/análise , Tumor do Seio Endodérmico/imunologia , Proteínas S100/análise , alfa-Fetoproteínas/análise , Adulto , Pré-Escolar , Desmoplaquinas , Feminino , Humanos , Imuno-Histoquímica , Imunofenotipagem , Lactente , MasculinoRESUMO
There are similarities between clear cell epithelial ovarian carcinoma and endodermal sinus tumours. Apart from the morphological and clinical characteristics there are immunohistochemical markers of value in differentiating these 2 tumours and the detection of a raised serum AFP is characteristic of endodermal sinus tumours. These 3 cases we describe show the fallibility of the classical differentiating criteria between these two tumours.
Assuntos
Adenocarcinoma de Células Claras/diagnóstico , Tumor do Seio Endodérmico/diagnóstico , Neoplasias Ovarianas/diagnóstico , Adenocarcinoma de Células Claras/imunologia , Adenocarcinoma de Células Claras/patologia , Adulto , Antígeno Ca-125/sangue , Diagnóstico Diferencial , Tumor do Seio Endodérmico/imunologia , Tumor do Seio Endodérmico/patologia , Feminino , Humanos , Imuno-Histoquímica , Antígenos CD15/análise , Pessoa de Meia-Idade , Neoplasias Ovarianas/imunologia , Neoplasias Ovarianas/patologia , alfa-Fetoproteínas/análiseRESUMO
A Gross virus induced rat T cell lymphoma G1-Tc1 and a Moloney virus induced mouse T cell lymphoma YAC-1 are shown to exert a strong cytotoxic activity against rat yolk sac tumours but not to various types of rat, mouse or human normal cells or tumour cell lines including carcinomas, sarcomas, lymphomas and gliomas. Both lymphomas are CD3+, CD4-, CD8- and T-cell receptor (TCR) alpha beta +. The cytotoxicity was not MHC restricted or dependent on the density of MHC class I of the target cells, and the mouse lymphoma killed the rat yolk sac tumour target. The cytotoxic action was fast and up to 80% specific killing was observed in 4-h 51Cr release assays. A rat B cell hybridoma was established from a Wistar/Furth (WF) rat immunized with the syngeneic lymphoma G1-Tc1 producing an immunoglobulin (Ig)G2c monoclonal antibody (MoAb) 1F2. This binds to the lymphomas G1-Tc1 and YAC-1 and also to a murine non-cytolytic Rauscher lymphoma RMA, but not to any other of several rat, mouse or human cell types tested. The 1F2 completely inhibited the killing of rat yolk sac tumours by the two cytolytic lymphomas, but did not interfere with the killing mediated by natural killer (NK) cells or cytolytic lymphokine-activated killer (LAK) cells. Immunochemical analysis of solubilized cell membranes of the lymphoma G1-Tc1 demonstrates that the 1F2 antibody recognizes an epitope on a retroviral gp 70 envelope protein. This indicates that a retroviral protein is involved in the lytic activity of the two lymphomas.
Assuntos
Vírus AKR da Leucemia Murina/fisiologia , Citotoxicidade Imunológica , Tumor do Seio Endodérmico/imunologia , Linfoma de Células T/imunologia , Proteínas de Membrana/fisiologia , Vírus da Leucemia Murina de Moloney/fisiologia , Proteínas de Neoplasias/fisiologia , Células-Tronco Neoplásicas/imunologia , Proteínas Oncogênicas de Retroviridae/fisiologia , Proteínas do Envelope Viral/fisiologia , Vírus AKR da Leucemia Murina/genética , Vírus AKR da Leucemia Murina/imunologia , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/farmacologia , Linhagem Celular , Citotoxicidade Imunológica/efeitos dos fármacos , Epitopos/imunologia , Humanos , Linfoma de Células T/virologia , Proteínas de Membrana/genética , Proteínas de Membrana/imunologia , Camundongos , Vírus da Leucemia Murina de Moloney/genética , Vírus da Leucemia Murina de Moloney/imunologia , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/imunologia , Ratos , Ratos Endogâmicos WF , Proteínas Oncogênicas de Retroviridae/genética , Proteínas Oncogênicas de Retroviridae/imunologia , Proteínas do Envelope Viral/genética , Proteínas do Envelope Viral/imunologiaRESUMO
The pattern of cell surface antigen expression of a set of cell lines derived from human germ cell tumours and corresponding to various cell phenotypes found within these tumours was studied using immunofluorescence. Twenty-two different antibodies were used. Many of these antibodies have been noted to recognise epitopes that are either preferentially expressed by embryonal carcinoma (EC) cells, or by more differentiated cell types. Using scatter plots and rank correlations, 6 groups of antibodies were distinguished with respect to their staining patterns on the cell lines tested. Several antibodies showed a specific staining pattern in relation to the differentiation state of the cells. Two groups of antibodies included those recognising high m.w. glycoproteins (antibodies TRA-1-60, TRA-1-81, GCTM2, 3-177, K4 and K21) and the ganglioseries glycolipid antigens SSEA-3 and -4 (antibodies MC631 and MC813-70). These antibodies mostly stained EC cells but not other cell types, confirming previously published data. However, one of these groups, comprising antibodies K4 and MC631, was more exclusively associated with the EC cell phenotype than was the other group. Antibodies recognising the liver isozyme of alkaline phosphatase (TRA-2-49 and TRA-2-54) also reacted strongly with most EC cell lines, although they reacted significantly with a number of other cell lines as well, whereas antibodies to the placental isozyme tended to react only weakly with EC cells. The antibodies recognising the ganglioseries glycolipids GD2 and GD3 (VIN2PB22 and VINIS56) preferentially stained cells with neuroectodermal characteristics. Other antibodies showed a heterogeneous staining pattern for the cell lines with different phenotypes. The data obtained from the cell lines were, in general, similar to data obtained from immunohistochemical studies on tissue sections of primary germ cell tumours of the adult testis, including carcinoma in situ.
Assuntos
Antígenos de Neoplasias/análise , Antígenos de Superfície/análise , Germinoma/imunologia , Neoplasias Testiculares/imunologia , Adulto , Fosfatase Alcalina/imunologia , Anticorpos Monoclonais/classificação , Anticorpos Monoclonais/imunologia , Anticorpos Antineoplásicos/classificação , Anticorpos Antineoplásicos/imunologia , Especificidade de Anticorpos , Biomarcadores Tumorais/imunologia , Biópsia , Carcinoma in Situ/química , Carcinoma in Situ/imunologia , Carcinoma in Situ/patologia , Carcinoma Embrionário/química , Carcinoma Embrionário/imunologia , Carcinoma Embrionário/patologia , Tumor do Seio Endodérmico/química , Tumor do Seio Endodérmico/imunologia , Tumor do Seio Endodérmico/patologia , Citometria de Fluxo , Técnica Indireta de Fluorescência para Anticorpo , Expressão Gênica , Germinoma/química , Germinoma/classificação , Germinoma/patologia , Glicolipídeos/imunologia , Humanos , Imunofenotipagem , Isoenzimas/imunologia , Masculino , Glicoproteínas de Membrana/imunologia , Microscopia de Fluorescência , Proteínas de Neoplasias/imunologia , Seminoma/química , Seminoma/imunologia , Seminoma/patologia , Neoplasias Testiculares/química , Neoplasias Testiculares/classificação , Neoplasias Testiculares/patologia , Testículo/patologia , Células Tumorais CultivadasRESUMO
A series of five endodermal sinus tumors was studied for their cytoskeletal and other phenotypic markers. They included 2 ovarian, 2 testicular, and 1 inguinal tumors. The cytoskeletal expression was also studied by gel electrophoresis and immunoblotting. Every tumor was diffusely and strongly immunostained for cytokeratin. By SDS-PAGE and immunoblotting, cytokeratins 8 & 18 were detected. Vimentin was focally coexpressed in 4 cases. The stroma was diffusely immunostained for vimentin. None of them expressed desmin, neurofilament, or glial filament protein. Desmoplakin was expressed only in one ovarian tumor. Alpha-fetoprotein and S-100 protein were also diffusely positive among the neoplastic cells; intracytoplasmic globules were especially strongly immunostained. These findings suggest that endodermal sinus tumors represent a group of pure malignant epithelial neoplasms, and may be regarded as primitive carcinomas.