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1.
Am J Med Genet A ; 179(9): 1756-1763, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31241255

RESUMO

Alpha-mannosidosis (AM) is a very rare (prevalence: 1/500000 births) autosomal recessive lysosomal storage disorder. It is characterized by multi-systemic involvement associated with progressive intellectual disability, hearing loss, skeletal anomalies, and coarse facial features. The spectrum is wide, from very severe and lethal to a milder phenotype that usually progresses slowly. AM is caused by a deficiency of lysosomal alpha-mannosidase. A diagnosis can be established by measuring the activity of lysosomal alpha-mannosidase in leucocytes and screening for abnormal urinary excretion of mannose-rich oligosaccharides. Genetic confirmation is obtained with the identification of MAN2B1 mutations. Enzyme replacement therapy (LAMZEDER ) was approved for use in Europe in August 2018. Here, we describe seven individuals from four families, diagnosed at 3-23 years of age, and who were referred to a clinical geneticist for etiologic exploration of syndromic hearing loss, associated with moderate learning disabilities. Exome sequencing had been used to establish the molecular diagnosis in five cases, including a two-sibling pair. In the remaining two patients, the diagnosis was obtained with screening of urinary oligosaccharides excretion and the association of deafness and hypotonia. These observations emphasize that the clinical diagnosis of AM can be challenging, and that it is likely an underdiagnosed rare cause of syndromic hearing loss. Exome sequencing can contribute significantly to the early diagnosis of these nonspecific mild phenotypes, with advantages for treatment and management.


Assuntos
Perda Auditiva/genética , Deficiência Intelectual/genética , alfa-Manosidase/genética , alfa-Manosidose/genética , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Perda Auditiva/sangue , Perda Auditiva/complicações , Perda Auditiva/patologia , Humanos , Deficiência Intelectual/sangue , Deficiência Intelectual/complicações , Deficiência Intelectual/patologia , Lisossomos/enzimologia , Masculino , Fenótipo , Irmãos , Sequenciamento do Exoma , Adulto Jovem , alfa-Manosidase/sangue , alfa-Manosidose/sangue , alfa-Manosidose/complicações , alfa-Manosidose/patologia
2.
Arch Neurol ; 43(2): 157-9, 1986 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-3947258

RESUMO

Three brothers with mannosidosis were assessed both biochemically by levels of enzyme activities and developmentally by serial testing of language and cognitive development. The findings indicated that while the leukocyte enzyme activity of alpha-mannosidase was exceptionally low, only mild intellectual deficits were present that did not progress during a two-year follow-up. These results do not substantiate the expected relationship between the severities of enzyme deficiency and developmental delays. Language and cognitive deficits appeared uniform with no areas of strengths or weaknesses. Deficits in development did not progress during a two-year follow-up.


Assuntos
Transtornos Cognitivos/diagnóstico , Transtornos do Desenvolvimento da Linguagem/diagnóstico , alfa-Manosidose/psicologia , Pré-Escolar , Transtornos Cognitivos/genética , Humanos , Lactente , Transtornos do Desenvolvimento da Linguagem/genética , Testes de Linguagem , Leucócitos/enzimologia , Masculino , Testes Psicológicos , alfa-Manosidose/sangue , alfa-Manosidose/enzimologia , alfa-Manosidose/genética
3.
Life Sci ; 47(7): 627-32, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-2402187

RESUMO

Slightly elevated serum dolichol levels have so far been demonstrated only in alcoholics. We now report two diseases with exceptionally high serum dolichol levels. They are autosomal, recessively inherited lysosomal storage diseases, aspartylglucosaminuria (AGU) and mannosidosis. In 16 patients with AGU the mean serum level of total dolichols (457 +/- 43 ng/ml) was more than two-fold when compared to healthy controls (170 +/- 4 ng/ml). In two patients with mannosidosis the levels were almost two-fold. The percentage distribution of the dolichol homologues with 18, 19 or 20 isoprene units did not differ between the patients and controls. The inclusion of an additional control group excluded the possible influence of mental retardation and imparied moving ability on the results. Elevated serum dolichols in patients with lysosomal storage diseases may reflect a disturbance in lysosomal function and serve as a diagnostic marker. The biochemical mechanisms leading to this phenomenon remain to be established.


Assuntos
Acetilglucosamina/análogos & derivados , Dolicóis/sangue , Glucosamina/análogos & derivados , Erros Inatos do Metabolismo/sangue , Acetilglucosamina/urina , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , alfa-Manosidose/sangue
4.
Eur J Pediatr ; 159(9): 699-703, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-11014473

RESUMO

UNLABELLED: Patients with the autosomal recessive lysosomal storage disease alpha-mannosidosis suffer from recurrent infections. To study the mechanisms of this immunodeficiency, six patients were matched against six healthy controls and their humoral and cellular immunocompetence investigated. No differences in the number of circulating leucocytes including B-cells, levels of immunoglobulin main classes, nor IgG subclasses were observed. However, post-immunisation serum levels of specific antibodies against poliovirus, diphtheria toxin and tetanus toxin were significantly reduced. In patients, the density of the complement-binding receptor CD11b and the Fc-receptor CD16 was significantly enhanced on monocytes and polymorphonuclear neutrophils (PMN) and the number of phagocytosing PMN was significantly increased in the presence of pooled human serum. This was not observed in the presence of autologous serum, indicating altered opsonic properties. Also in normal PMN, phagocytosis was inhibited by a factor in the serum from the patients. Despite maintained oxidative burst, patient PMN demonstrated insufficient intracellular bacterial killing. CONCLUSION: Our data indicate that patients with alpha-mannosidosis have an immunodeficiency at both the humoral and cellular level.


Assuntos
Síndromes de Imunodeficiência/complicações , alfa-Manosidose/imunologia , Adolescente , Adulto , Estudos de Casos e Controles , Criança , Proteínas do Sistema Complemento/análise , Feminino , Humanos , Imunoglobulinas/sangue , Síndromes de Imunodeficiência/sangue , Leucócitos/fisiologia , Masculino , Fagocitose , Receptores de Complemento/sangue , alfa-Manosidose/sangue , alfa-Manosidose/complicações
5.
Tidsskr Nor Laegeforen ; 115(5): 594-7, 1995 Feb 20.
Artigo em Norueguês | MEDLINE | ID: mdl-7900112

RESUMO

Alpha-mannosidosis is a rare autosomal recessively inherited lysosomal storage disorder. We describe three patients with alpha-mannosidosis who were born in Tromsø between 1983 and 1987, in order to increase awareness of the disease. It is characterized by a typical facial look, with a prominent forehead, hypertelorism, small nose, flat nasal bridge and hypoplastic teeth. The patients are mentally retarded, often have dysostosis multiplex, recurrent infections and typically severe loss of hearing and delayed speech development. The disease is slowly progressive in the first decade, but shows considerable clinical variability. In most cases, the lymphocytes are vacuolized, but diagnosis depends on measurement of alpha-mannosidase activity in the lymphocytes. Prenatal diagnosis is available, based on chorionic villi sampling in the 9th to 11th week of pregnancy. No causal therapy is known, but establishment of the diagnosis is important to avoid complications, recognize hearing loss and provide speech therapy and special education. The specific diagnosis is critical for genetic counselling and prenatal diagnosis. The authors therefore outline the diagnostic strategy.


Assuntos
alfa-Manosidose/diagnóstico , Anormalidades Múltiplas/diagnóstico , Anormalidades Múltiplas/genética , Criança , Pré-Escolar , Amostra da Vilosidade Coriônica , Diagnóstico Diferencial , Face/anormalidades , Feminino , Humanos , Diagnóstico Pré-Natal , Radiografia , Coluna Vertebral/anormalidades , Coluna Vertebral/diagnóstico por imagem , alfa-Manosidose/sangue , alfa-Manosidose/diagnóstico por imagem
6.
Vet Pathol ; 26(4): 294-302, 1989 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2503918

RESUMO

The morphology and ultrastructure of circulating white blood cells from six Persian and from five Russian Blue/Siamese cats deficient in lysosomal activity of alpha-mannosidase and arylsulfatase B, respectively, were studied and compared to cells from corresponding normal and carrier cats. In cats with mannosidosis, light microscopic examination revealed vacuoles in lymphocytes and monocytes, whereas electron microscopic studies demonstrated additional vacuoles in neutrophils, eosinophils, and basophils. In cats with mucopolysaccharidosis VI (MPS VI), vacuoles containing metachromatic granules were observed in lymphocytes, neutrophils, eosinophils, and monocytes. Ultrastructural studies of these cells identified the accumulation of fibrillar material, which often was associated with lamellated membrane structures.


Assuntos
Doenças do Gato/sangue , Leucócitos/ultraestrutura , Mucopolissacaridoses/veterinária , Mucopolissacaridose VI/veterinária , alfa-Manosidose/veterinária , Animais , Basófilos/ultraestrutura , Gatos , Condro-4-Sulfatase/sangue , Eosinófilos/ultraestrutura , Leucócitos/enzimologia , Linfócitos/ultraestrutura , Lisossomos/enzimologia , Manosidases/sangue , Mucopolissacaridose VI/sangue , Neutrófilos/ultraestrutura , alfa-Manosidase , alfa-Manosidose/sangue
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