Insights into antimicrobial peptides from Limacus flavus Mucus

ABSTRACT

Antimicrobial peptides have been a major research subject since the rise of antimicrobial resistance as a major public health problem. These molecules are considered a potential therapeutic source of antibiotics with broad-spectrum activity against microorganisms. Two antimicrobial peptides were isolated from the mucus of the Limacus flavus slug. The mucus was obtained by thermal shock, lyophilized and extracted with acetic acid. The supernatant was prefractionated in Sep-Pak and shortly thereafter fractionated by reverse-phase high-performance liquid chromatography. The manually obtained fractions were used in antimicrobial and cytotoxic assays and finally subjected to mass spectrometry (MS/MS). Characterization was performed by bioinformatics analysis with the tool Peaks®X + and by comparison with the NCBI and UniProt-SwissProt databases. Additionally, the physicochemical parameters of the samples were evaluated with online programs. Two fractions comtained antimicrobial peptides with the ability to inhibit Micrococcus luteus A270; both samples, LFMP-001 and LFMP-002, were hydrophilic molecules consisting of fewer than 20 residues. Comparison of the SDS-PAGE and Peaks®X + data showed that both had Mw < 3 kDa. In summary, this study presents data on the isolation and characterization of antimicrobial peptides from a slug and shows their potential against gram-positive bacteria.