Polysaccharide-Specific Memory B Cells Predict Protection against Experimental Human Pneumococcal Carriage
Am. J. Respir. Crit. Care Med.
; 194(12): p. 1523-1531, 2016.
Article
| SES-SP, SESSP-IBPROD, SES-SP
| ID: but-ib14635
Biblioteca responsable:
BR78.1
Ubicación: BR78.1
ABSTRACT
Rationale We have previously demonstrated that experimental pneumococcal carriage enhances immunity and protects healthy adults against carriage reacquisition after rechallenge with a homologous strain. Objectives:
To investigate the role of naturally acquired pneumococcal protein and polysaccharide (PS)-specific immunity in protection against carriage acquisition using a heterologous challenge model.Methods:
We identified healthy volunteers that were naturally colonized with pneumococcus and, after clearance of their natural carriage episode, challenged them with a heterologous 6B strain. In another cohort of volunteers we assessed 6BPS-specific, PspA-specific, and PspC-specific IgG and IgA plasma and memory B-cell populations before and 7, 14, and 35 days after experimental pneumococcal inoculation. Measurements and MainResults:
Heterologous challenge with 6B resulted in 50% carriage among volunteers with previous natural pneumococcal carriage. Protection from carriage was associated with a high number of circulating 6BPS IgG-secreting memory B cells at baseline. There were no associations between protection from carriage and baseline levels of 6BPS IgG in serum or nasal wash, PspA-specific, or PspC-specific memory B cells or plasma cells. In volunteers who did not develop carriage, the number of circulating 6BPS memory B cells decreased and the number of 6BPS plasma cells increased postinoculation.Conclusions:
Our data indicate that naturally acquired PS-specific memory B cells, but not levels of circulating IgG at time of pneumococcal exposure, are associated with protection against carriage acquisition
Texto completo:
1
Colección SES:
Producao_cientifica
Banco de datos:
SES-SP
/
SESSP-IBPROD
Asunto principal:
Patología
/
Neumología
/
Alergia e Inmunología
Año:
2016
Tipo del documento:
Article