Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis
Mem. Inst. Oswaldo Cruz
; 97(suppl.1): 115-116, Oct. 2002. ilus
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| LILACS
| ID: lil-325028
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BR1.1
ABSTRACT
Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females), Echinostoma paraensei, and Taenia saginata - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests
Texto completo:
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Banco de datos:
LILACS
Asunto principal:
Schistosoma mansoni
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Esquistosomiasis mansoni
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Proteínas del Helminto
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Western Blotting
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Electroforesis en Gel de Poliacrilamida
Límite:
Animals
Idioma:
En
Año:
2002
Tipo del documento:
Article
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Congress and conference