Differential effects of manipulating signaling in early T cell development in intestinal intraepithelial lymphocytes and thymocytes.
Int Immunol
; 11(2): 169-77, 1999 Feb.
Article
en En
| MEDLINE
| ID: mdl-10069415
ABSTRACT
A pre-TCR-CD3 signal is required for the efficient maturation of CD4- CD8- thymocytes to the CD4+ CD8+ stage. This study addressed whether a similar signal is required for maturation of intestinal intraepithelial lymphocytes (IEL) that may develop extrathymically. We have shown previously that IEL from mice deficient for CD3- associated zeta chains include an immature population of CD3- CD8alphaalpha+ cells expressing cytoplasmic TCR beta chains but lacking detectable surface TCRalphabeta, CD16 and B220. Here we stimulated the appearance of such IEL in epsilon+/- zeta-/- mice by expression of an activated Lck transgene or in vivo treatment with anti-CD3epsilon. Anti-CD3epsilon treatment of RAG-deficient animals also yielded CD16- B220- IEL. In contrast, expression of a TCRbeta transgene in rag-1(-/-) mice did not stimulate the appearance of CD3- CD8alphaalpha+ CD16- B220- cells. Taken together these data indicate that although anti-CD3epsilon treatment and LckF505 assist in catalyzing a CD16+ B220+ --> CD16- B220- transition, these manipulations are not equivalent to a pre-TCR signal in IEL lymphocytes.
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Banco de datos:
MEDLINE
Asunto principal:
Timo
/
Linfocitos T
/
Transducción de Señal
/
Mucosa Intestinal
Límite:
Animals
Idioma:
En
Año:
1999
Tipo del documento:
Article