Contributions of the ionization states of acidic residues to the stability of the coiled coil domain of matrilin-1.
FEBS Lett
; 446(1): 75-80, 1999 Mar 05.
Article
en En
| MEDLINE
| ID: mdl-10100618
ABSTRACT
The pKa values of eight glutamic acid residues in the homotrimeric coiled coil domain of chicken matrilin-1 have been determined from 2D H(CA)CO NMR spectra recorded as a function of the solution pH. The pKa values span a range between 4.0 and 4.7, close to or above those for glutamic acid residues in unstructured polypeptides. These results suggest only small favorable contributions to the stability of the coiled coil from the ionization of its acidic residues.
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Banco de datos:
MEDLINE
Asunto principal:
Conformación Proteica
/
Glicoproteínas
/
Proteínas de la Matriz Extracelular
Límite:
Animals
Idioma:
En
Año:
1999
Tipo del documento:
Article