Characterization of the isoenzyme profile of beta-N-acetylhexosaminidase in the urine of newborns.
Clin Chem Lab Med
; 37(7): 765-9, 1999 Jul.
Article
en En
| MEDLINE
| ID: mdl-10510735
ABSTRACT
The urinary isoenzymes of beta-N-acetylhexosaminidase (Hex) in newborn infants were characterised by chromatography, electrophoresis, thermodynamic analysis and through substrate specificity. No qualitative difference was found for the major Hex A and Hex B isoenzymes between full-term or premature newborns and adults, although in the latter group the relative proportion of Hex B is much lower (18.5 +/- 2.7% vs. 36.3 +/- 1.0%). An additional minor enzyme form was found in some premature newborns, which eluted from the DEAE-cellulose column at a higher concentration of NaCl than Hex A and, like this isoenzyme, is able to hydrolyse 4-methylumbellipheryl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6 -sulphate, which would suggest that it has alpha subunits in its molecule. These results do not confirm the hypothesis of other authors about the existence of a unique fetal Hex isoenzyme in neonatal urine which eluted before the application of the NaCl gradient, similarly to the Hex B.
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Banco de datos:
MEDLINE
Asunto principal:
Beta-N-Acetilhexosaminidasas
/
Recién Nacido
Tipo de estudio:
Qualitative_research
Límite:
Adult
/
Humans
Idioma:
En
Año:
1999
Tipo del documento:
Article