Characterization of boar spermadhesins by monoclonal and polyclonal antibodies and their role in binding to oocytes.
Am J Reprod Immunol
; 42(3): 187-97, 1999 Sep.
Article
en En
| MEDLINE
| ID: mdl-10517179
ABSTRACT
PROBLEM:
The role of Ala-Trp-Asn (AWN) and Ala-Gln-Asn (AQN) families of spermadhesive sperm proteins in fertilization. METHOD OF STUDY The preparation and characterization of polyclonal antibodies against AWN and AQN spermadhesins and one monoclonal antibody (MAb), designated Bo.5, against AWN spermadhesin. The use of biochemical and immunocytochemical methods for characterization of spermadhesins on the sperm membrane of boar spermatozoa and in the cryostat sections of boar reproductive organs.RESULTS:
Polyclonal anti-AWN and anti-AQN antibodies specifically reacted with AWN and AQN proteins, respectively. MAb Bo.5 detected the 17-, 16-, and 14-kDa protein members of AWN subfamily. The monoclonal, as well as the polyclonal, AWN antibodies remarkably decreased the sperm binding to the egg surface in an in vitro sperm zona pellucida binding assay.CONCLUSIONS:
Presented results demonstrate that polyclonal antibodies and MAb Bo.5 against spermadhesins specifically recognize the membrane-associated antigens and inhibit the binding of sperm to oocytes. Reduced binding of sperm to oocytes, due to the antibodies, indicates the role of these spermadhesins in sperm-egg primary binding.
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Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Interacciones Espermatozoide-Óvulo
/
Espermatozoides
/
Sitios de Unión de Anticuerpos
/
Glicoproteínas
/
Moléculas de Adhesión Celular
/
Anticuerpos Monoclonales
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Año:
1999
Tipo del documento:
Article