Identification of differentially expressed genes in Con A-activated carp (Cyprinus carpio L.) leucocytes.
Comp Biochem Physiol B Biochem Mol Biol
; 124(1): 41-50, 1999 Sep.
Article
en En
| MEDLINE
| ID: mdl-10582319
ABSTRACT
A cDNA library was constructed from the message RNA (mRNA) obtained from Con A-induced head kidney (HK) leucocytes of carp (Cyprinus carpio L.). Differential screening of the cDNA was carried out by hybridization against the total cDNA probes from normal, Con A-uninduced HK leucocytes or Con A-induced HK leucocytes of carp. The differential expression patterns of certain cDNA clones were confirmed by Southern-blot and Northern-blot analysis. Single-pass of the sequencing analysis and homology search in Genbank (EMBL) revealed those differentially expressed cDNA clones encode for cytochrome c oxidase sub-unit II and III (COII and COIII), elongation factor-1 beta (EF-1 beta), bleomycin hydrolase (BH), heat shock cognate protein 70 (HSC70) and 16S ribosomal RNA (16S rRNA).
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Banco de datos:
MEDLINE
Asunto principal:
Carpas
/
Activación de Linfocitos
/
Proteínas HSP70 de Choque Térmico
/
Concanavalina A
/
Genes
/
Leucocitos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Año:
1999
Tipo del documento:
Article