[Examining interaction of phages with microorganisms by fluorometry and electro-orientation spectroscopy]. / Izuchenie vzaimodeistvii fagov i mikroorganizmov s ispol'zovaniem metodov fliuorimetrii i élektroorientatsionni spektroskopii.
Vestn Ross Akad Med Nauk
; (12): 24-9, 1999.
Article
en Ru
| MEDLINE
| ID: mdl-10709463
ABSTRACT
Bacterial sensitivity to different various phages was examined by electro-orientation spectroscopy, fluorometry, and electron microscopy. The strains of Pseudomonas aeruginosa, Staphylococcus aureus, Yersinia pestis, Mycobacterium smegmatis, and Xanthomonas campestris were used. The fluorescence intensity of a membranotropic agent in the ANS-cell-phage system was shown to depend on the interaction of a bacterial virus and a microorganism. Fluorometric data correlated with electro-orientation spectroscopic findings. An analysis of the low-frequency site makes it possible to determine phage adsorption on the bacterial surface. The changes in electro-orientation effects at high frequencies suggest that there are barrier dysfunctions in the external membranes and that there is cellular phage reproductions. Whether fluorometry and electro-orientation spectroscopy can be further used for rapid identification of microorganisms by using phages is discussed.
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Banco de datos:
MEDLINE
Asunto principal:
Pseudomonas aeruginosa
/
Staphylococcus aureus
/
Bacteriófagos
/
Yersinia pestis
/
Xanthomonas campestris
/
Mycobacterium
Tipo de estudio:
Prognostic_studies
Idioma:
Ru
Año:
1999
Tipo del documento:
Article