An improved protocol for the preparation of protoplasts from an established Arabidopsis thaliana cell suspension culture and infection with RNA of turnip yellow mosaic tymovirus: a simple and reliable method.
J Virol Methods
; 86(1): 85-94, 2000 Apr.
Article
en En
| MEDLINE
| ID: mdl-10713379
ABSTRACT
An improved method for preparation of protoplasts of Arabidopsis thaliana cells grown in suspension culture is presented. This method is fast, reliable and can be used for the production of virtually an unlimited number of protoplasts at any time. These protoplasts can be transformed efficiently with RNA from turnip yellow mosaic tymovirus (TYMV) by polyethyleneglycol-mediated transfection. The simple transfection procedure has been optimized at various steps. Replication of TYMV can be monitored routinely by detection of the coat protein in as few as 2 x 10(4) infected protoplasts.
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Banco de datos:
MEDLINE
Asunto principal:
Protoplastos
/
Transfección
/
Arabidopsis
/
Tymovirus
Idioma:
En
Año:
2000
Tipo del documento:
Article