Capillary electrophoresis of DNA damage after irradiation: apoptosis and necrosis.
J Chromatogr A
; 871(1-2): 321-30, 2000 Feb 25.
Article
en En
| MEDLINE
| ID: mdl-10735312
ABSTRACT
Apoptosis is a type of cellular death but also directly regulates tumorigenesis through different gene expression. This phenomenon is often used as end-point in studies of radio- and chemosensitivity of cancer cells. Restriction DNA fragments have been separated quickly, efficiently and successfully by capillary gel electrophoresis (CGE). In this study CGE has been applied to distinguish between the discrete pattern of degraded DNA produced by apoptosis and randomized DNA breaks produced by ionizing radiation. The influence of different variables has been discussed and an example of fast separation by CGE of the apoptotic fragments produced by UV light treatment is shown.
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Banco de datos:
MEDLINE
Asunto principal:
Daño del ADN
/
ADN
/
Apoptosis
/
Electroforesis Capilar
Tipo de estudio:
Clinical_trials
Idioma:
En
Año:
2000
Tipo del documento:
Article