Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75.
Bull Exp Biol Med
; 77(7): 792-4, 1975 Jan.
Article
en En
| MEDLINE
| ID: mdl-1090313
ABSTRACT
A method of separating rosette-forming cells on columns with an immunosorbent based on sephadex G-75 is described and has been tested. The protein antigen (bovine serum albumin-BSA) is attatched by covalent bonds to the surface of the Sephadex granules oxidized by sodium periodate. Cells with receptors on their surface were tested by the rosette method. Up to 88% of the rosette-forming cells were retained on a column packed with BSA-Sephadex granules. Nonspecific retention of the cells was relatively small, about 4%.
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Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Linfocitos
/
Separación Celular
Límite:
Animals
Idioma:
En
Año:
1975
Tipo del documento:
Article