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Growth hormone regulates growth hormone receptor gene transcription in primary human thyroid cells.
Mullis, P E; O'Donovan, N; Eblé, A; Marti, U; Diem, P; Bürgi, U; Peter, H J.
  • Mullis PE; Department of Paediatrics, Division of Paediatric Endocrinology, University Children's Hospital, Inselspital, CH-3010, Bern, Switzerland. primus.mullis@insel.ch
Mol Cell Endocrinol ; 166(2): 111-9, 2000 Aug 30.
Article en En | MEDLINE | ID: mdl-10996429
ABSTRACT
In this study the regulation of GH-receptor gene (GHR/GHBP) transcription by different concentrations of GH (0, 12.5, 25, 50, 150, 500 ng/ml) with and without variable TSH concentrations (0.5, 2, 20 mU/l) in primary human thyroid cells cultured in serum-free hormonally-defined medium was studied. The incubation time was 6 h and GHR/GHBP mRNA expression was quantitatively assessed by using PCR amplification at hourly intervals. Correlating with the GH-concentrations added a constant and significant increase of GHR/GHBP gene transcription was found. After the addition of 12.5 ng/ml GH, GHR/GHBP mRNA concentration remained constant over the incubation period of 6 h but in comparison with the experiments where no GH was added there was a significant change of GHR/GHBP mRNA expression. Following the addition of 25 ng/ml GH a slight but further increase of GHR/GHBP transcription products was seen which increased even more in the experiments where higher GH concentrations were used. These data focusing on GHR/GHBP gene transcription derived from cDNA synthesis and quantitative PCR amplification were confirmed by run-on experiments. Furthermore, cycloheximide did not affect these changes supporting the notion that GH stimulates GHR/GHBP gene transcription directly. In a second set of experiments, in combination with variable TSH levels, identical GH concentrations were used and no difference in either GHR/GHBP mRNA levels or in transcription rate (run-on experiments) could be found. In conclusion, we report data showing that primary thyroid cells express functional GH-receptors in which GH has a direct and dose dependent effect on the GHR/GHBP gene transcription. Furthermore, TSH does not a have a major impact on GHR/GHBP gene regulation.
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Banco de datos: MEDLINE Asunto principal: Glándula Tiroides / Receptores de Somatotropina / Hormona de Crecimiento Humana Límite: Humans Idioma: En Año: 2000 Tipo del documento: Article
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Banco de datos: MEDLINE Asunto principal: Glándula Tiroides / Receptores de Somatotropina / Hormona de Crecimiento Humana Límite: Humans Idioma: En Año: 2000 Tipo del documento: Article