The BLAST algorithms: practical application in molecular cloning, marker-assisted selection (MAS) and introgression of wheat.
DNA Seq
; 11(3-4): 339-47, 2000.
Article
en En
| MEDLINE
| ID: mdl-11092750
ABSTRACT
BLAST (Basic Local Alignment Search) analyses of Hordeum chilense RAPD markers have shown DNA sequence similarities with several plant genes. Biologically significant hits were scored for plastocianin (Hordeum vulgare), alpha-gliadin (Triticum aestivum), Grandel-6 retrotransposon (Zea diploperennis), retrofit (gag/pol) copia-like, transposon-like element (Oryza longistaminata), copia-like retrotransposon Hopscotch polyprotein (gag/pol) (Zea mays) and several retrotransposons/retroelements from other species (Arabidopsis thaliana, Oryza sativa, Pisum sativum and Zea mays). Statistically significant hits also included endochitinase (Brassica napius), ribulose-1,5-bisphosphate carboxylase (Euglena gracilis), piruvate, orthophosphate dikinase (Flaveria trinervia), and an ORF flanked by ARS sequences (Triticum aestivum). Interestingly, it was also found that the currently recommended BLAST algorithm (version 2.0.11; gapped) at
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Banco de datos:
MEDLINE
Asunto principal:
Hordeum
/
Triticum
/
Marcadores Genéticos
/
Bases de Datos Factuales
/
Clonación Molecular
/
Genes de Plantas
/
ADN de Plantas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2000
Tipo del documento:
Article