Determination of sperm acrosin activity for evaluation of male fertility.
Asian J Androl
; 2(3): 229-32, 2000 Sep.
Article
en En
| MEDLINE
| ID: mdl-11225983
ABSTRACT
AIM:
To investigate a simple method for assaying acrosin activity for the evaluation of male fertility.METHODS:
The acrosin activity of 7.5 x 10(6) sperm without seminal plasma and acrosin activity inhibitors was assayed using N-alpha-benzoyl-DL-arginine-p-nitroanilide (BAPNA) and detergent (Triton X-100) as substrate.RESULTS:
The acrosin activity of 60 normal fertile men (35 +/- 10 microIU/10(6) sperm ) was higher than that of 168 infertile men (16 +/- 8 microIU/10(6) sperm) (P < 0. 01). It was indicated that there was a significant positive correlation between the acrosin activity and the sperm motility (r > or = 0.6534, P < 0.01) and a significant negative correlation between the sperm malformed rate and the WBC number (r < or = -0.5426, P < 0.01). The temperature and time of incubation and the sperm concentration could influence the assay results.CONCLUSION:
Acrosin activity is an important index for the evaluation of male fertility. The approach developed by the authors is a simple method for the determination of acrosin activity.
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Banco de datos:
MEDLINE
Asunto principal:
Semen
/
Acrosina
/
Fertilidad
/
Infertilidad Masculina
Límite:
Adult
/
Humans
/
Male
Idioma:
En
Año:
2000
Tipo del documento:
Article