Role of screening agar plates for in vitro susceptibility testing of Helicobacter pylori in a routine laboratory setting.
J Clin Pathol
; 54(5): 408-11, 2001 May.
Article
en En
| MEDLINE
| ID: mdl-11328845
ABSTRACT
BACKGROUND:
Resistance of Helicobacter pylori to the more frequently used antibiotics (metronidazole and clarithromycin) reduces eradication rates even with triple treatment. Determining the antibiogram profile of H pylori can take up to 14 days and delays appropriate treatment.AIMS:
To determine the role of screening agar plates for more rapid in vitro susceptibility of H pylori to metronidazole, amoxicillin, and clarithromycin.METHODS:
Routine gastric biopsy specimens from 507 dyspeptic patients were inoculated on to 10% lysed blood agar plates containing metronidazole (8 microg/ml), clarithromycin (2 microg/ml), or amoxicillin (0.5 microg/ml). The minimum inhibitory concentration (MIC) of the 90 isolates was determined using the E test.RESULTS:
Metronidazole resistance was detected in 28 of 90 isolates by E test and nine of 98 by screening agar. The screening agar detected none of the four clarithromycin resistant isolates detected by the E test.CONCLUSIONS:
The screening agar method is not sufficiently sensitive to be used alone.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pruebas de Sensibilidad Microbiana
/
Helicobacter pylori
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Adult
/
Humans
Idioma:
En
Año:
2001
Tipo del documento:
Article