Thalidomide and its analogues inhibit lipopolysaccharide-mediated Iinduction of cyclooxygenase-2.
Clin Cancer Res
; 7(11): 3349-55, 2001 Nov.
Article
en En
| MEDLINE
| ID: mdl-11705847
ABSTRACT
We investigated the effect of thalidomide, a compound with immunomodulatory and antiangiogenic properties, on lipopolysaccharide (LPS)-mediated induction of cyclooxygenase-2 (Cox-2) and prostaglandin (PG) biosynthesis in murine macrophages. Thalidomide caused a dose-dependent inhibition of LPS-mediated induction of PGE(2) synthesis in RAW 264.7 cells. The induction of Cox-2 protein and mRNA by LPS was also suppressed by thalidomide. Based on the results of nuclear run-off assays and transient transfections, treatment with LPS stimulated Cox-2 transcription, an effect that was unaffected by thalidomide. Thalidomide decreased the stability of Cox-2 mRNA. A series of structural analogues of thalidomide also inhibited LPS-mediated induction of Cox-2 and PGE(2) synthesis. Taken together, these data provide new insights into the antineoplastic and anti-inflammatory properties of thalidomide.
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Banco de datos:
MEDLINE
Asunto principal:
Talidomida
/
Lipopolisacáridos
/
Prostaglandina-Endoperóxido Sintasas
/
Isoenzimas
Límite:
Animals
Idioma:
En
Año:
2001
Tipo del documento:
Article