Automated quantitation of hemoglobin-based blood substitutes in whole blood samples.
Am J Clin Pathol
; 116(6): 913-9, 2001 Dec.
Article
en En
| MEDLINE
| ID: mdl-11764082
ABSTRACT
It is necessary to develop methods for accurate monitoring of cell-free hemoglobin in circulation. Routine monitoring of circulating cell-free hemoglobin will be useful for evaluating the efficacy of blood substitute administration andfor determining the clearance rates of the blood substitute from circulation. In addition, discriminating between cell-free hemoglobin and cell-associated hemoglobin will enable accurate determination of RBC indices, mean cell hemoglobin and mean corpuscular hemoglobin concentration, in individuals receiving hemoglobin-based blood substitutes. As colorimetric methods used by hematology analyzers to quantitate the hemoglobin value of a blood sample cannot distinguish between cell-associated and cell-free hemoglobin, it is currently not feasible to quantitate the levels of hemoglobin substitutes in circulation. The advent of a technology that measures volume and hemoglobin concentration of individual RBCs provides an alternative strategy for quantitating the cell-associated hemoglobin in a blood sample. We document that the combined use of cell-based and colorimetric hemoglobin measurements provides accurate discrimination between cell-associated and cell-free hemoglobin over a wide range of hemoglobin levels. This strategy should enable rapid and accurate monitoring of the levels of cell-free hemoglobin substitutes in the circulation of recipients of these blood substitutes.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Sustitutos Sanguíneos
/
Hemoglobinas
/
Eritrocitos
/
Hemoglobinometría
Límite:
Humans
Idioma:
En
Año:
2001
Tipo del documento:
Article