[Detection of the expression of alpha3-integrin on hantavirus permissive cells].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 16(1): 40-3, 2002 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-11986744
ABSTRACT
BACKGROUND:
To express and purify human alpha3-integrin to serve as the antigen to prepare its antibody and to separate the Vero cell clones without expression of alpha3-integrin.METHODS:
The human alpha3-integrin gene was amplified by using RT-PCR, then subcloned into a pQE30 expression vector and expressed in E. coli. The gene expression was confirmed by Western blot assay. Rabbit was inoculated with purified antigen to stimulate the antibody generation. The target Vero cells were separated by negative selection using antibody plus complement mediated cytolysis. The separated cell clones were confirmed by immunofluorescence and Western blot assay.RESULTS:
The alpha3- integrin gene was cloned and expressed effetively, Western blot assay revealed that the expressed protein held good immune reactivity. High titer antibody was generated. However the expression of alpha3-integrin was not detected on Vero, VeroE6, Hep-2, 2BS and 293 cells.CONCLUSIONS:
The results of the study suggested that hantavirus has other receptors on Vero cells beside alpha 3-integrin.
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Banco de datos:
MEDLINE
Asunto principal:
Orthohantavirus
/
Integrina beta3
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Año:
2002
Tipo del documento:
Article