The effect of calcium on the tryptic digestion of bovine intestinal calcium-binding protein.
Biochim Biophys Acta
; 412(2): 256-61, 1975 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-1238120
ABSTRACT
The tryptic hydrolysis of bivine intestinal calcium-binding protein in the presence and absence of excess calcium has been investigated. Calcium-binding activity and immunological reactivity of the protein were not significantly affected in the presence of 1.0 mM CaCl2 following 24 h incubation at 38 degrees C with trypsin at ratios of 19 of enzyme to calcium-binding protein. Some modification of the protein did occur under these conditions, however, since analysis by analytical acrylamide gel electrophoresis indicated the formation of a more rapidly-migrating species from the slower-moving original protein band. Omission of added calcium from the incubation medium resulted in rapid and essentially complete destruction of calcium-binding activity and immunological reactivity, and the formation of peptides of low molecular weight. This provides evidence that the conformation of the calcium-binding protein in the presence of calcium differs from that in its absence.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Tripsina
/
Proteínas Portadoras
/
Calcio
Límite:
Animals
Idioma:
En
Año:
1975
Tipo del documento:
Article