Fluorescence imaging of signaling networks.
Trends Cell Biol
; 13(2): 101-6, 2003 Feb.
Article
en En
| MEDLINE
| ID: mdl-12559761
ABSTRACT
Receptor-triggered signaling processes exhibit complex cross-talk and feedback interactions, with many signaling proteins and second messengers acting locally within the cell. The flow of information in this input-output system can only be understood by tracking where and when local signaling activities are induced. Systematic strategies are therefore needed to measure the localization and translocation of all signaling proteins, and to develop fluorescent biosensors that can track local signaling activities in individual cells. Such a biosensor tool chest can be based on two types of green fluorescent protein constructs that either translocate or undergo fluorescence-resonance-energy transfer when local signaling occurs. Broad strategies to measure quantitative, dynamic parameters in signaling networks, together with perturbation approaches, are needed to develop comprehensive models of signaling networks*.
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Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Sistemas de Mensajero Secundario
/
Transducción de Señal
/
Receptores de Superficie Celular
/
Transporte de Proteínas
/
Células Eucariotas
Límite:
Animals
/
Humans
Idioma:
En
Año:
2003
Tipo del documento:
Article